Table of Content

18 October 2018, Volume 35 Issue 5 Previous Issue    Next Issue

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The research advance of IL-33′s role in cancer QI Lu, MIAO Yu-hui, XIAO Wei-hua, FANG Fang 2018, 35 (5):  1.  Abstract ( 424 )   PDF   Save Interleukin-33 (IL-33), as interleukin-1 like cytokine, plays important roles in type Ⅱ immune responses. Recent studies suggest that IL-33 involves in tumor progression through modulating immune response, but the roles and mechanism are still controversial. These diverse results are likely due to the tissue origin, stage and microenvironment of the tumor, which implicates that IL-33 acts multiple roles in a context-dependent manner. To reveal the distinct roles of IL-33 in tumor development, further studies should be performed in more refined conditions. The profound understanding of IL-33 function in tumor progression is necessary for its application in clinical diagnosis and treatment. Related Articles | Metrics

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Study on the splicing mechanism of Cne PRP8 intein with directional evolution LI Shu-meng, LI Man, MENG Qing 2018, 35 (5):  8.  Abstract ( 294 )   PDF   Save Intein-mediated protein splicing offers many promises in protein engineering. However, the mechanisms of protein splicing is still unclear, which greatly limits the general application of protein splicing in protein engineering. Cne PRP8 intein from a eukaryotic genome had been improved through directed evolution consisting of random mutagenesis followed with functional selection. This improved Cne PRP8 intein will be subjected to site-specific mutations of amino acid residues at critical locations, followed with restoration mutation, and mutational studies of extein amino acid residues at the splice junctions, in order to discover new mechanisms of the protein splicing. The results show that the splicing of eukaryotic protein intein is still related to its one site and its positive one site, but for the intein of Cne PRP8 protein, its splicing efficiency is related to its the 52th site, 68th site,114th site and 146th site amino mutations, and the mutations of its amino acids change the polarity, thus affecting the splicing of protein introns. This study not only consolidates the mechanism of protein intron splicing, but also reveals the evolutionary pathway of protein introns in eukaryotes. Related Articles | Metrics

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Effect of HaV01 Pol internal cysteines to its splicing activity LI Jia, MENG Qing 2018, 35 (5):  13.  Abstract ( 280 )   PDF   Save The purpose of this study is to obtain a Cysteine-free intein as a biological tool for labeling or modification. Four Cysteines in HaV01 Pol were mutated and replaced by site-directed mutation and over-lap PCR, and their splicing activity was detected by Western Blot. The results showed that the 112th Cysteine negatively regulates the splicing activity of HaV01 Pol but not other three Cysteines. In addition, replacement of the 112th Cysteine with Glycine and Threonine can rescue the splicing efficiency up to 50%. Surprisingly, this is the first time to report one Cysteine localized at the unconserved block which can determine the intein′s splicing activity. In the end, two Cysteine free HaV01 Pol mutants were constructed which are facilitated for HaV01 Pol′s further transformation as well as application. Related Articles | Metrics

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Cloning and expression analysis of thioredoxin-like 2 gene (DoTrxL2) in Dendrobium officinale Kimura et Migo FAN Jing, MAO Huan, LIU Chu-qi, LONG Tao, ZHOU Kang, WANG Wan-jun 2018, 35 (5):  18.  Abstract ( 233 )   PDF   Save The thioredoxin-like 2 gene (DoTrxL2) in Dendrobium officinale Kimura et Migo was cloned by RACE and nest PCR technologies. The full length of DoTrxL2 contained an open reading frame (ORF) of 639 bp encoding 213 amino acid residues, whose product contained a conserved CXXC domain. Phylogenetic analysis showed that the DoTrxL2 had the closest relationship with monocotyledons, such as Phalaenopsis equestris and Asparagus officinalis. qRT-PCR analysis showed that the expression levels of DoTrxL2 were significantly varied in different developmental stages of protocorm and different tissues, which indicated that the DoTrxL2 plays an important role in the development of protocorm and the morphogical establishment of tissues in plant. Related Articles | Metrics

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Conditioned medium from bladder cancer cell YTS-1 induces EMT-like  phenotype and altered glycan profile in normal epithelial HCV29 cells XU Xiao-qiang;GUO Jia; GUAN Feng 2018, 35 (5):  23.  Abstract ( 424 )   PDF   Save Epithelial mesenchymal transition (EMT) is a key process in cancer development. Communication between cancer cells and normal cells may facilitate cancer progression. Conditioned medium (CM) from cultured cancer cells containing secreted factors may affect behaviors of normal cells.In this study, normal bladder epithelial HCV29 cells were cultured with CM from malignant bladder cancer YTS-1 cells (Y-CM). In comparision with control cells, HCV29 treated with Y-CM showed the increased expression of mesenchymal markers fibronectin and N-cadherin enhanced cell proliferation, migration,and inhibition of cell apoptosis. Furthermore, N-glycan profiles of Y-CM treated and control HCV29 were compared by MALDI-TOF-MS and lectin microarray. The treated cells showed lower levels of high-mannose type N-glycan structures,and higher levels of complex-type, hybrid-type, fucose-type and sialic acid-type structures. Taken together, our findings indicated that CM derived from bladder cancer cells induced an EMT-like process in normal bladder epithelial cells and altered their N-glycan profile. Related Articles | Metrics

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The influence of MGAT3-catalyzed bisecting GlcNAc in stroma cells on cell adhesion and migration of hematopoietic cells ZHANG Xin, PANG Xing-chen, LI Xiang 2018, 35 (5):  28.  Abstract ( 291 )   PDF   Save Bone marrow stromal cells are one of the important components of hematopoietic microenvironment, and play a very important role in regulating hematopoiesis. In this study, we inhibited the expression of bisected N-glycan in stroma cells and observed its effect on cell adhesion and migration of hematopoietic cells in co-culture system. N-acetylglucosaminyltransferase-III (so called MGAT3)， the key enzymes in the synthesis of N-glycan was silenced in human stroma cells HS5 using genetic engineering techniques. We found the level of bisecting GlcNAc significantly decreased in HS5 after MGAT3 was inhibited. Cell adhesion and migration of hematopoietic cells KG1a and PL-21 decreased after co-cultured with HS5 that inhibitg the expression of bisecting GlcNAc. Our data indicated that bisecting GlcNAc in stroma cells had important roles in regulating hematopoietic cells adhesion and migration. Related Articles | Metrics

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Phylogenetic information analysis of mitochondrial genome sequences in Siganus (Perciformes: Siganidae) HUANG Xiao-lin, YANG Yu-kai, LIN Hei-zhao, LI Tao, YU Wei, HUANG Zhong 2018, 35 (5):  33.  Abstract ( 284 )   PDF   Save DNA complete sequences of 6 Siganus were analyzed, and the basic structure, variation site, gene selection pressure and phylogenetic evolution of mtDNA in Siganus were revealed. mtDNA in Siganus was composed of 37 genes, among which, the highest proportion of variation site was ND3 gene (22.77%), and the minimum proportion was 12S rRNA (4.01%). The mtDNA protein coding genes (13)of Ka/Ks were far less than 1, and Tajima′s D=0.123 74~0.615 14 (P>0.10)were not significantly greater than or less than 0, which showed that the stability (negative)selection of mtDNA protein coding genes in Siganus was stronger during its evolutionary process. Phylogenetic analysis showed that S. unimaculatus and S. vulpinus were firstly clustered into one group, and then in turn clustered with S. puellus and S. guttatus, finally clustered into one group with S. fuscescens and S. canaliculatus. This evolutionary order is consistent with changes in body color of Siganus. COX1, ND4 and Cytb genes are ideal molecular markers for phylogenetic evolution in Siganus. Related Articles | Metrics

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Effects of Pb2+ on the activities of five mitochondrial enzymes in mouse liver LIU Gang, DU You-wen, ZHANG Lu, LIU Xiao-ying 2018, 35 (5):  37.  Abstract ( 309 )   PDF   Save Mitochondrial enzymes play important role for cell, but the morphological characteristics, structure and function of enzymes would be changed significantly stressed by heavy metals, and also will be irreversible damaged. This study takes the liver mitochondria of mice as the subject of study under the 50mg/kg, 100mg/kg, 200mg/kg, 300mg/kg, 400mg/kg and 500mg/kg of different concentrations of Pb2+ stress to explore the five mitochondrial enzyme activities. The results showed that SDH, NADH, ATP, GST and GSH-PXG activity decreased as Pb2+ dosage increased. And all reached the bottom after 500mg/kg, which had significant differences compared with controls (P<0.01). Statistical analysis showed that SDH, NADH, ATP, GST and GSH-PX activity had a significant negative correlation with Pb2+ concentration. Related Articles | Metrics

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Identification and histopathological analysis of the pathogenic bacteria  in the yellow gill disease of Litopenaeus Vannamei CHEN Zheng-tao, ZHAO Lin-ying, SHEN Wei-liang, XU Sheng-wei, JING Zhong-wen, CHEN Cai-fang 2018, 35 (5):  41.  Abstract ( 311 )   PDF   Save  In the artificial culture of Litopenaeus vannamei, we found yellow gill disease， resulting in great loss.In order to study the major pathogens causing the yellow gill disease in Litopenaeus vannamei, PCR and 16S rDNA sequencing techniques were used to identify the pathogens isolated from diseased Litopenaeus vannamei, and the pathogenicity of major pathogens was determined by artificial infection, paraffin section was used to observe histopathological lesions in gill tissues. Finally, the drug sensitivity of pathogenic bacteria was determined by drug sensitivity test. The results showed that the yellow gill disease in Litopenaeus vannamei caused by Vibrio shilonii and Exiguobacterium together. In addition, histopathological examination showed enlarged gill filament, diffussion and erosion of tissue in gill. The antibiotic susceptibility test indicated that the strain Vibrio shilonii was sensitive to pipemidic acid, ofloxacin, flumequine, etc., but resistant to sulfadimidine and ampicillin. While, the strain Exiguobacterium was sensitive to ampicillin sodium, ofloxacin, trimethoprim, etc., but resistant to sulfadimidine and ampicillin. This obtained information will provide a theoretical basis for studying the pathogenesis and treatment of yellow gill disease in Litopenaeus vannamei. Related Articles | Metrics

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Expression and construction of RNA interference vector of MADS-box family gene AGL13 from Brassica napus XIONG Shu,SUN Hou-liang, YANG Li-shan, MA Qiang, LI Guo-li, ZHOU Da-xiang 2018, 35 (5):  45.  Abstract ( 256 )   PDF   Save In this study, the tissue expression pattern of AGL13 was analyzed by qPCR, and the RNA interference vector was constructed to explore the molecular regulation mechanism of oil synthesis in seed of Brassica napus to increase the oil content. Bioinformatics analysis showed that AGL13 protein of Brossica napus has complete and typical MADS-box conserved domain. The overall shape of protein showed typical DNA nucleic acid binding structure. The AGL13 belonged to the MADS-box family transcription factor, and genetic relationship was the closest with AGL13 protein of Arabidopsis. The qPCR study found that AGL13 expression was very high from the flowering stage to the seed 45DAP (ays seed after 45 d flowering), was highest in the fruit clamp 15DAP. The RNA interference vector was successfully constructed in the experiment. The results showed that the AGL13 gene was strongly expressed in seed development and oil accumulation, which may play an important role in the seed development and oil synthesis. The successful construction of RNA interference vector would be favourable for obtaining the transgenic B. napus, and observing the seed development and oil accumulation of transgenic B. napus. Thus determining the AGL13 gene plays an important role in the seed development and oil synthesis. Related Articles | Metrics

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Research on maize system resistance triggered by the  endophytic bacterium DZSY21 SUN Huang-bing, ZHONG Nian-xiao, KONG Ling-ru, DING Ting, MA Yuan-shan 2018, 35 (5):  49.  Abstract ( 241 )   PDF   Save Preliminary tests showed the endophytic bacterium DZSY21 isolated from Eucommia ulmoides could colonize in maize, and the resistance of maize to Bipolaris maydis was enhanced by colonization of DZSY21. In this study, the resistance-related enzymes activity and signal transduction pathways were investigated to clarify the mechanism of maize systemic resistance triggered by the endophytic bacterium DZSY21. After leaves treated with DZSY21, the activities of PPO, POD and PAL of the maize leaves increased at 3-10 days. Meanwhile, the defense gene PR-1 in salicylic acid(SA) signaling pathway and the gene LOX in jasmonic acid (JA) signaling pathway were expressed continuously at 24-60 h and 12-60 h, respectively. Accordingly, the DZSY21 was capable of inducing systemic resistance in maize plants. In summary, the application of the endophytic bacterium DZSY21 could trigger maize systemic disease resistance which plays an important role in the control of Bipolaris maydis. Related Articles | Metrics

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Cytological observation of meiotic chromosome behavior in the synthetic Brassica napus（AACC） TIAN Yan-fei, ZHU Li-li, XU Cong-cong, YANG Yan, WEI Fang, TIAN Bao-ming 2018, 35 (5):  54.  Abstract ( 266 )   PDF   Save To investigate the meiotic course of synthetic Brassica napus at a cytological level, we analyzed the chromosomal behavior of pollen mother cells of resynthesized B.napus and cultivated B.napus (AACC).The results showed that the number of chromosomes in resynthesized B.napus was 2n=38, the same as the cultivated B. napus. However, in the resynthesized B.napus the multivalents formed at diakinesis, chromosomes aligned chaotically at metaphase I, chromosome lags were frequently observed from later metaphase I to anaphaseⅡ. The cells with abnormal meiosis at metaphaseⅠ and anaphaseⅠ were 48.93% and 22.12%, respectively. In this study, chromosome behavior during meiosis analyzed at a cytological level in the synthetic B. napus would provid cytogenetic evidences for plant breeding in Brassica napus by artificial methods in future. Related Articles | Metrics

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New distribution of Saxifraga species in Qinghai Province GENGJI Zhuoma, LI Yan, JIA Liu-kun, CHEN Shi-long, GAO Qing-bo 2018, 35 (5):  58.  Abstract ( 392 )   PDF   Save Due to the complex diversity in ecology, morphology and cytology of Saxifraga, it is difficult to identify the species, which results to an incomplete collection in local floras. Thirty-two Saxifraga species were captured in Flora Qinghaiica. New distribution of six Saxifraga L. species in Qinghai Province was discovered based on field expedition and specimen identification, namely Saxifragaangustata, S. atuntsiensis, S.congestiflora, S. granulifera, S. heleonastes and S. pasumensis, which are neither reported in Flora Qinghaiica nor in Flora of China. Related Articles | Metrics

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The prediction of honeybee colony swarming based on the sub-band power ratio of colony sound LYU Zhu-qing 2018, 35 (5):  61.  Abstract ( 314 )   PDF   Save Beehive sound-based monitoring the states of a honeybee colony, including health and preswarming, is an important means for precise beekeeping. However, the present classifiers of a beehive sound are mostly developed according to the multiple features of the beehive sound using complex algorithms of machine learning. In the study, the "artificial swarming" method to get the beehive swarming was used. The beehive sounds of Apis cerana were recorded and analyzed under preswarming and non-preswarming conditions, and the power spectral density and the sub-band power ratio of the sounds were extracted. The results showed that the frequency ranges of the non-preswarming beehive sound were mainly within 0-200 Hz, while those of the preswarming beehive sound within 200-400 Hz. Based on the sub-band power ratio of the beehive sound, a machine learning classifier was developed for predicting the preswarming state of a honeybee colony with a CART decision tree algorithm, and a higher prior probability of 99.04% was achieved. This study would provide a new feature for developing the classifiers of a beehive sound. Related Articles | Metrics

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The establishment of intrafusal muscle cell calcium overload model of soleus muscle in rats YU Lei, ZHAO Xue-hong, FAN Xiao-li, CAO Jian, ZHU Juan-xia 2018, 35 (5):  65.  Abstract ( 233 )   PDF   Save The experiment was used Ca2+ import agent Ionomycin to establish intrafusal muscle cell calcium overload model of soleus muscle in rats. The confocal laser scanning microscopy was adopted to observe the effects of different concentrations of Ionomycin on resting intracelluar calcium in intrafusal fibers of rats′ soleus muscle. After administration of 2 mol/L Ionomycin, the resting intracelluar calcium in intrafusal fibers of rats′ soleus muscle increased slowly（P<0.01）, and (2.67±0.43) min later after reaching the peak value, the maximum value of MFI decreased by 50%. After administration of 5 μmol/L Ionomycin, the resting intracelluar calcium in intrafusal fibers of rats′ soleus muscle increased rapidly （P<0.01）, and (6.26±0.87) min later after reaching the peak value, the maximum value of MFI decreased by 50%. Ionomycin of 2 μmol/L can form a short-term calcium overload in intrafusal cells. Ionomycin of 5 μmol/L can establish a stable intrafusal muscle cell calcium overload model of soleus muscle in rats. This study provides valuable information for the study of rat muscle spindle physiology and the experimental study of calcium and weightlessness. Related Articles | Metrics

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MSW reduction research based on multi-factor sensitivity analysis ——a case study of Hefei in China WANG Hao,XIE Jia-feng,WANG Qian,LIU Wei, JIN Jie 2018, 35 (5):  69.  Abstract ( 289 )   PDF   Save The article analyzes the factors sensitivity of Hefei MSW(municipal solid waste) by use of the sensitive factor method. Aiming at the problem of Hefei city life garbage output grows day by day， we summarizes the factors which influence the production of urban living garbage through literature review. Based on this， we build the AHP structure diagram to analyze the MSW factors， then using multivariate regression model to determine the main factors that influence the Hefei MSW. By analyzing the size of its sensitivity， we come to the most sensitive factors of Hefei MSW. At last， we propose some countermeasures to reduce Hefei MSW. Related Articles | Metrics

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Research progress in the structure and function of rotavirus NSP5#br# LIU Yang, LIN Xiao-chen, LI Hong-jun 2018, 35 (5):  72.  Abstract ( 525 )   PDF   Save Rotaviruses (RV) are leading causes of gastrointestinal diseases in infants and young children. The RV genome consists of 11 subunits of double-stranded RNA, encoding six structural proteins (VP1, VP2, VP3, VP4, VP6, VP7) and six non-structural proteins (NSP1, NSP2, NSP3, NSP4, NSP5, NSP6), where the 11th gene fragment encodes the nonstructural protein NSP5. NSP5 protein is large, in the form of dimer which in the virus-infected cells, and is a highly phosphorylated protein, with rotavirus other non-structural protein or structural protein interaction, with RNA binding activity, in the virus replication process, mainly through a series of interactions with NSP2 to play a function. In recent years, a large number of non-structural proteins of rotavirus have been studied, mainly in its structure, expression, function and immunological properties, mthis study; the progress the structure and function of rotavirus NSP5 at home and abroad was reviewed. Related Articles | Metrics

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Microenvironment of cancer stem cell and the regulation strategy HAO Fang-fang, ZHAO Tong-jun, FENG He, WANG Jian-li, ZHAN Yong 2018, 35 (5):  75.  Abstract ( 409 )   PDF   Save Cancer stem cells (CSCs) can self-renew and differentiate into different germline cancer cells, while the specific microenvironment of CSCs is essential for the division of CSCs. The spread of cancer and the tumor recurrence are highly related to the microenvironment of CSCs, and the construction of efficient tumor targeting therapy relies on the adequate understanding about this regulation mechanisms. Therefore, this review mainly focuses on the latest findings in the field of CSCs microenvironment, especially in the research of colorectal cancer, breast cancer, leukemia and inoculated tumor. Related Articles | Metrics

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Research progress of DNA methylation sequencing technology and application in mammal DUAN Xin-yu, XIAO Heng, CHEN Shan-yuan 2018, 35 (5):  79.  Abstract ( 338 )   PDF   Save DNA methylation is an important epigenetic mark in many eukaryotes. The quantitative measurement of mammal′s genome-wide DNA methylation level has become a research hotspot by combining with the next-generation sequencing (NGS) technologies. With the advantages of high-throughput, high coverage and high resolution through the comparison with other sequencings, NGS technologies provide an important approach for accurately detecting DNA methylation sites within the whole genome. In order to provide a reference for the selection of DNA methylation sequencing technology, three methods of restriction endonuclease digestion, affinity enrichment and bisulfite conversion were summarized and compared in this paper, and the future development of sequencing technology was predicted. Related Articles | Metrics

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Effects of intestinal microbiota on lung diseases E Yuan-yuan,ZHU Li-meng,LI Wen-jun,ZHAI Shi-xiang,QIN Song 2018, 35 (5):  83.  Abstract ( 339 )   PDF   Save The human intestinal is dominated by hundreds of different species of bacteria. It is important for maintaining human health.Intestinal microbial community dysbiosis is related to obesity,diabetes and pulmonary diseases. In recent yeas, more and more studies on intestinal microbiota and pulmonary diseases are reported. Research indicates that respiratory disease is usually accompanied by a bowel syndrome, and vice versa. In addition, respiratory diseases such as allergic asthma and pneumonia seriously affect human health. Intestinal microbiota on host immune regulation and the role of metabolites are crucial for the treatment of lung disease. In this paper, we summarized approaches of intestinal microbiota as targets for prevention and treatment of pulmonary diseases, aimed at providing new ideas for the future prevention and treatment of lung diseases. Related Articles | Metrics

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Review on multiplex polymerase chain reaction for the detection of genetically modified components LU Jun,LI Gang,ZHAO Jian-ning,YANG Dian-lin,XIU Wei-ming 2018, 35 (5):  87.  Abstract ( 293 )   PDF   Save  As the planting area of the global genetically modified crops has bear increasing rapidly and the commercialized process is accelerated, people have paid more attention to the safety of genetically modified plants and derived products. The supervision of genetically modified plants and derived products has been paid more attention by many countries and areas. The accurate, efficient, and inexpensive detection methods of genetically modified components are the important technical support for supervision and labeling of genetically modified food and feed. Multiplex polymerase chain reaction (Multiple PCR) technology has captured the attention of more and more researchers and has been popularized and applicated in many kinds of industries because of its low cost, high flux, better specificity, saving the experimental samples and medicine. This paper showes the latest research progress on multiple PCR detection method combined with other technology and the application to different detect requirement of genetically modified ingredients. The paper also gives further strategies for improving Multiple PCR detection methods of genetically modified components． Related Articles | Metrics

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Functional studies of arabinogalactan proteins on root morphological formation in higher plant ZENG Wei, JIANG Ai-xi, TU Jin-hua, TANG Xing-chun 2018, 35 (5):  91.  Abstract ( 387 )   PDF   Save Arabinogalactan proteins (AGPs) are complex proteoglycans found in the entire plant kingdom, implicating that biological functions of which are involved in various aspects of cell proliferation, cell division， abiotic stress， sexual reproductive development and embryogenesis. In this review, we summarized their specific features of AGPs, and then focused on current knowledge of their functional properties, interactions between plant and environment in root tissues. Related Articles | Metrics

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Research advances in medicinal plants of Cynomorium songaricum REN Meng-yun, YANG Guang, DU Le-shan, LIU Fang, ZHANG Dun,SHEN Qi, GUAN Xiao, ZHANG Yin-dong 2018, 35 (5):  95.  Abstract ( 467 )   PDF   Save Cynomorium songaricum Rupr. is a well-known achlorophyllous holoparasite medicinal psammophyte, which is usually parasitic on the roots of Nitraria plants in the family Zygophyllaceae. Cynomorium songaricum, a kind of rare and endangered medicinal plants, was widely used as Chinese, Mongolian and Tibetan herbal remedy for problems such as renal disease, climacteric syndrome, and sexual dysfunction, runchang catharsis and lumbar weakness. In this paper, we reviewed the biological characteristics, genetic diversity, chemical constituents, pharmacological effects and artificial cultivation techniques of C.songaricum and put forward the research focus and hot spots on this basis. What we should focuses on is the pharmacological mechanism of C.songaricum bioactive components, establishing a complete evaluation system of the Herba C.songaricum quality. We need study C. songaricum germplasm resources, protection and regeneration deeply, strengthen the artificial breeding technique of C.songaricum for its protection. Related Articles | Metrics

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Application of algal functional material in agricultural production LI Qian, YANG Rui, SUN Hui, CHEN Hai-min 2018, 35 (5):  99.  Abstract ( 296 )   PDF   Save Seaweeds are important marine resource rich in polysaccarides, oligosaccharides, polyphenols, plant hormones, pigments, and many other active substances. The seaweeds have diverse biological functions like providing nutrition, keeping water moisture for plants, promoting plant growth and reproduction, and improving the resistance ability to disease and stress in plants. The yield and quality of crops also raise because of the effects of seaweeds productions. The algal functional materials have been applied in many agriculture fields as fertilization, pesticide, moisturizer, elicitor and hormone et al. In this paper, the description, preparation techniques and the application of algal functional materials in agriculture were reviewed, and the potential commercial development of applying the algal functional materials in agriculture were discussed too. Related Articles | Metrics

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An overview on biology and artificial propagation and culture in the spotted scat Scatophagus argus YANG Wei, CHEN Hua-pu, JIANG Dong-neng, DENG Si-ping, WU Tian-li, ZHU Chun-hua, LI Guang-li 2018, 35 (5):  104.  Abstract ( 443 )   PDF   Save Spotted scat (Scatophagus argus), which has the notable ability to tolerate a wide range of environmental salinities, is widely distributed around the Indo-Pacific region. S. argus with such features as high nutritional value, deliciousness, easy cultivation, low feeding cost, etc. is increasingly becoming an important farmed fish with considerable economic value in South and South-East Asia, including southeast coastal areas of China. The commercial demand for seedlings is rising sharply. In order to provide useful information and suggestion for the further development of germplasm resource and aquaculture industry of S. argus, this article reviewed and summarized the research findings and progress in basic biology, reproductive biology, molecular genetics, artificial propagation and culture. Furthermore, the future development was analyzed and discussed, and the directions of future research were proposed tentatively. Related Articles | Metrics

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Stimulating innovation thinking of graduate student and exploring reform of advanced microbiology YU Xiu-mei， GU Yun-fu， XIANG Quan-ju， ZHAO Ke， CHEN Qiang， MA Meng-gen 2018, 35 (5):  109.  Abstract ( 265 )   PDF   Save Innovation is the driving force of scientific research. Innovation thinking is the necessary requirement of the cultivation of high-quality scientific research personal. According to graduate students showing low research interest, lacking innovation thinking, low scientific research quality during the process of graduate education and major feature of Microbiology and development direction of subject, the education of "Advanced Microbiology" performs reform. The course reform of young teachers, curriculum professionalization, diverse teaching, flexible examination and so on performs to train students′ innovation thinking, which will export high-level personnel with high research quality and strong comprehensive ability for development of Chinese science and technology. Related Articles | Metrics

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The exploration and deliberation of food microbiology experiment teaching based on the cultivation of innovation capacity ZHU Ben-wei, SUN Yun, YAO Zhong 2018, 35 (5):  113.  Abstract ( 237 )   PDF   Save In order to promote the learning interest and enthusiasm of the students, the authors tried to introduce the teaching content and methods aimed at cultivating the students′ innovation capacity, and launched the exploration and reformation of the food microbiology experiment teaching by means of experiment design, experiment content and evaluating method. Additionally, the importance of cultivation of innovation capacity has been analyzed in this course system. Related Articles | Metrics

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Construction of experiment center of biotechnology & biomaterial and experimental training system WANG Zhao, WANG Jin-ke 2018, 35 (5):  116.  Abstract ( 262 )   PDF   Save This paper introduces the construction of experiment center of biotechnology and biomaterial of our University, and expatiates on the construction of experimental teaching system based on this platform from the aspects of curriculum arrangement, course content, teaching materials compose and teaching effect evaluation. These works provide an experimental teaching center with advanced teaching equipment, excellent teaching environment, complete teaching content, and excellent teaching team for biomedical engineering undergraduate and graduate students. It is more conducive to the development of biomedical engineering and biomedical engineering personnel training in the future. Related Articles | Metrics

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Reform and exploration of molecular biology experiment teaching for undergraduate LI Hui-min, QIN Xin-min, DENG Ting 2018, 35 (5):  120.  Abstract ( 235 )   PDF   Save The technique of molecular biology is an important means in molecular biology research. Therefore, molecular biology experiment teaching is as important as theoretical teaching for undergraduate in higher education. In view of the students learning initiative and enthusiasm was very low, this paper focused on reforming teaching modes, optimizing the experiment teaching content and exploring the student assessment method in molecular biology experimental teaching for undergraduate. It showed that students′ experimental skills were strengthened after practice. Meanwhile, the quality of molecular biology experiment teaching was improved to a certain extent and further fostered undergraduates’ comprehensive abilities of analyzing and solving problems. Related Articles | Metrics

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Analysis of the training of biology education graduate students in China LI Zeng-jiao, XU Min-hua, ZHANG Yue-ming, ZHENG Xiao-hui 2018, 35 (5):  123.  Abstract ( 203 )   PDF   Save In the past ten years, the number of biological education graduate students has been increasing. However, the socially recognized quality of postgraduate and the topic of dissertation closely related to the educational reform were not yet quantified. Based on bibliometrics, the scientific knowledge mapping and keywords, a statistical analysis and visualization was made. Results showed that the studies of senior high school were more; and so were the quantitative methods; hot topics were affected by the education reform and curriculum standard. This paper puts forward some suggestions that the topic of dissertation should pay more attention to the cultivation of all-round development of students and to the depth of quantitative research and combination with qualitative research, and learn to study education from the interdisciplinary point of view. Related Articles | Metrics

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Teaching exploration in “introduction to enviromental science” curriculum for non-environment majors under new engineering disciplines background: a case study of electronic information majors CHEN Chen, LI Yan-yan, HU Xue-you 2018, 35 (5):  127.  Abstract ( 269 )   PDF   Save New engineering disciplines require strengthening the ecological consciousness of engineering students."Introduction to enviromental science" as a general course for non-environment majors should be improved and explorated in combination with teaching of new engineering disciplines characteristics, following national policies and technology development, enriching teaching methods and perfecting practical teaching activities. It can take resopnsibility for cultivating students′ environmental protection and ecological consciousness to adapt to the new mode of talent training under new engineering disciplines background. Related Articles | Metrics