Abstract: Bone marrow stromal cells are one of the important components of hematopoietic microenvironment, and play a very important role in regulating hematopoiesis. In this study, we inhibited the expression of bisected N-glycan in stroma cells and observed its effect on cell adhesion and migration of hematopoietic cells in co-culture system. N-acetylglucosaminyltransferase-III (so called MGAT3)， the key enzymes in the synthesis of N-glycan was silenced in human stroma cells HS5 using genetic engineering techniques. We found the level of bisecting GlcNAc significantly decreased in HS5 after MGAT3 was inhibited. Cell adhesion and migration of hematopoietic cells KG1a and PL-21 decreased after co-cultured with HS5 that inhibitg the expression of bisecting GlcNAc. Our data indicated that bisecting GlcNAc in stroma cells had important roles in regulating hematopoietic cells adhesion and migration.

Key words: MGAT3, bisecting GlcNAc, stromal cell, hematopoietic cell HS5, cell adhesion, cell migration