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γH2AX， 53BP1 and RAD51 foci analysis for monitoring DNA double-strand breaks WANG Yi-zhao, FAN Li, WANG Hong-xia, YANG Si-lu, CUI Ya-ni, Duan Yi-jun, Duan Jing-jing, REN Lai-feng, SU Wen Journal of Biology    2020, 37 (1): 16-.   DOI: 10.3969/j.issn.2095-1736.2020.01.016 Abstract （5699）      PDF       Knowledge map    γH2AX, 53BP1 and RAD51 foci are the evaluation markers of DNA double strand breaks (DSBs). Above all, γH2AX foci analysis is the most widely used, but recent studies have found that it is not completely consistent with DSBs damage. This study systematically analyzed the dynamic changes of γH2AX, 53BP1 and RAD51 foci during DNA damage and their reasonable application in DSBs damage assessment. According to the results, γH2AX and 53BP1 foci were highly consistent after cells exposed to ionizing radiation treatment, they both could reflect accurately the dynamic changes of DSBs damages and repairs; while RAD51 foci only appeared in part of damaged cells, which was consistent with its role mainly involved in homologous recombination process. Upon treatment with Camptothecin (CPT), γH2AX presented two types of staining (bright and discrete foci and pan-nuclear phosphorylation), while 53BP1 foci only appeared in cells with bright and discrete γH2AX foci and were highly consistent with γH2AX foci. These results revealed that positive staining of γH2AX alone cannot accurately represent DSBs damage, and the extent of DSBs damage should be comprehensively analyzed by combining with staining of 53BP1. The RAD51 foci analysis only reflect the DSBs damage repair of part cells, mainly homologous recombination repair.  Related Articles | Metrics

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Effects of Different Stocking Densities and Diets on growth and survival of Eriocheir sinensis in Larval Stages YANG zhi-gang1, LIU Qi-bin1, YAO Qin-qin1, Yang Qing1, WANG Jian-yi1, CHENG Yong-xu1, YANG Xiao-zhen1, GE Yong-chun2

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Research progress on key metabolic enzymes in de novo synthesis pathway of ceramide LI Yu-hui, CHEN Cheng Journal of Biology    2020, 37 (4): 90-.   DOI: 10.3969/j.issn.2095-1736.2020.04.090 Abstract （1787）      PDF       Knowledge map    Ceramide is an important active lipid that regulates cell differentiation, proliferation, apoptosis, aging, tumor immunity and other vital life activities, and it is also a universal precursor for a series of important physiological sphingolipids such as glycosphingolipids. As the center of sphingolipid metabolism, the syntheses and degradations of ceramide in the body are strictly regulated. Its syntheses include de novo synthesis pathway, sphingomyelinase pathway and remedy pathway. Although de novo synthesis pathway contributes only 5%-10% to the synthesis of ceramide in the body, recent studies on key metabolic enzymes in this pathway have shown that they are closely related to many diseases, which also illustrates the complexity and importance of this pathway in the life of the organism. The purpose of this article is to systematically review the research progress of key metabolic enzymes in de novo synthesis, in order to provide a theoretical reference for further understanding of this pathway and the functions of key metabolic enzymes, and also lay an foundation for the development of personalized medicine for this pathway in the future. Related Articles | Metrics

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Study on adsorption property of Cr6+ by sycamore bark activated carbon WANG Lei,WEI Xin-lai,JIN Jie,LI Cong,WANG Pei-yang

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The research progress of Toll-like receptors in innate immune system#br# ZHOU Qing, HAO Lu, ZHOU Ze-qiang

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Reform of experimental teaching based on organic combination of virtual experiment and real experiment CHEN Su-yun, LIN Zhen-ting

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Commuinity dynamics of phytoplankton and related affecting factors in Shengjin Lake WU Fang-yi, WANG Lan, XU Mei, WANG Hui, ZHOU Zhong-ze

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Study on the selectivity of Carassius auratus under different environment colors and luminous intensities GONG Jian-hua, GUO Chun-yang, TIAN Zhe, XU Shan-liang

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Lilium concolor var. Pulchellum: a new distribution plant in Anhui, China FENG Jun-ru, GAN Ting-ting, FENG Shuang, YIN Hua-bao

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Bioinformatics analysis of Shigella flexneri 2a outer membrane protein A and molecular designing of recombinant epitope vaccine LIU Xiang

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Composition and diversity of small mammals community in Shilong, Jianchuan area of Yunnan Province ZHU Wan-long, ZHANG Hao, MENG Li-hua, CAI Jin-hong, WANG Zheng-kun

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The comparison between two states of Oujiang color common carp，Cyprinus carpio var. color scales XU Hui-bin, ZHANG Qian, WANG Cheng-hui, GONG Xiao-ling

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The inhibitory effect of resveratrol in hepatocellular carcinoma Smmc-7721 cells LI Lu, WANG Lu, CHEN Guang-liang, HUANG Mei-xian, SONG Rui, WEI Qiang

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The research progress in pepsin purification and enzymatic activity#br# LIU Yu, DONG Ping, LIANG Xing-guo

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Rho/ROCK signaling pathway and its active regulation mode CAO Mei-Xia1， 2， QU Chang-Qing1

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Morphological characteristics of digestive tract in Plagiognathops microlepis MU Dan-li, YUAN Si-ping, XUE Cong-shun, WU Zhong-ning, ZHU Jun-quan

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Cloning and expression stability evaluation of reference genes in Spanish Mackerel (Scomberomorus niphonius) ZHANG Jia-ling, XUE Liang-yi, SHI Jun-xing

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Molecular cloning, sequence analysis and expression analysis of ApoC-Ⅰgene from ayu (Plecoglossus altivelis) exposed to cadmium CHEN Ya-ping, DING Fei-fei, LI Chang-hong, MO Li-bin, CHEN Jiong

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Antimicrobial Effects and Mechanisms of Natural Plant Antimicrobial Solution against Staphylococcus aureus Bi Zhen-fei, Song ming-zhu, Liu Zong-ying, Zhou Yi-jie, Li di-qiang, Mao Can-quan

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Genetic diversity of Larimichthys crocea based on mitochondrial DNA control region sequences in three cultured populations SHEN jing-yi，XUE liang-yi，SHEN wei-liang，HE meng-meng