Table of Content

18 April 2025, Volume 42 Issue 2 Previous Issue   

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Enzymatic synthesis of (S)-3-hydroxytetrahydrofuran LI Runtong, LI Xu, YUAN Bo, GUO Jinggong, QU Ge, SUN Zhoutong 2025, 42 (2):  1.  doi: 10.3969/j.issn.2095-1736.2025.02.001 Abstract ( 42 )   PDF   Save (S)-3-Hydroxytetrahydrofuran is an important pharmaceutical intermediate, and its chemical synthesis meets challenges such as low stereoselectivity and high cost. Alternatively, alcohol dehydrogenases catalyze the one-step asymmetric reduction of dihydro-3(2H)-furanone to the corresponding alcohol product. In this study, after screening an alcohol dehydrogenase library, an alcohol dehydrogenase derived fromAromatoleum aromaticumEbN1 (PED) was obtained, which enabled the reduction of dihydro-3(2H)-furanone to (S)-3-hydroxytetrahydrofuran. By optimizing the reaction conditions, PED catalyzed the model reaction with a >99% conversion. The catalytic efficiency (kcat/Km) was measured at 0.40 L/（mmol·s）, with aneevalue of 90%. Additionally, PED demonstrated a good thermostability, maintaining aTmvalue of 67 ℃. Computational analysis revealed the mechanism of stereoselectivity in the reduction of substrates catalyzed by PED. The key residues such as S141, Y151 and Y154 formed a network of hydrogen-bonding interactions with the substrate, which promoted the formation of apro-(S) conformation of the substrate. The (S)-product was then formed by the migration of a hydride from the coenzyme NADH to the carbonyl carbon of the substrate. Furthermore, large-scale reactions showed that PED can completely convert the substrate concentration up to 400 mmol/L in 5 h, with an isolated yield of 74.7% and aneevalue of 90%. In this study, a novel alcohol dehydrogenase PED was identified and characterized with enzymatic performance, expanding the repertoire of enzymes available for the biocatalysis of (S)-3-hydroxytetrahydrofuran. Related Articles | Metrics

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In vitro spontaneous conformational transitions between G-quadruplexes formed by the guanine-rich DNA fragment d（G3AG3T2G3A2G3）upstream of promoter E1 in the human proto-oncogene c-fms LIU Zhiyue, JING Haitao, ZHU Ting, FU Wenqiang, ZHANG Na, HU Wenxuan 2025, 42 (2):  7.  doi: 10.3969/j.issn.2095-1736.2025.02.007 Abstract ( 39 )   PDF   Save F3g is a short DNA fragment, d(G3AG3T2G3A2G3), locating upstream of promoter E1 in the human proto-oncogenec-fms. Nuclear magnetic resonance (NMR) studies have revealed that, in near-physiological K+solutions, F3g folds into two distinct G-quadruplex structures with a spontaneous folding topology switch. Subsequent validations using a combination of techniques including NMR, CD, and PAGE have demonstrated that the kinetically favored Form 1 structure was a non-parallel two-tetrad G-quadruplex, while the thermodynamically stable Form 2 structure consisted of a stack of two parallel three-tetrad G-quadruplexes. These findings provided insights and a theoretical basis for further understanding the expression regulation of the human proto-oncogenec-fmsand the targeted treatment of related diseases. Related Articles | Metrics

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Static magnetic field combined with metformin improves glucose metabolism and gut microbiota in T2D mice CHENG Guofeng, WANG Ying, FENG Chuanlin, LIAO Zhongcai, WEI Min, FANG Yanwen, ZHANG Xin 2025, 42 (2):  15.  doi: 10.3969/j.issn.2095-1736.2025.02.015 Abstract ( 32 )   PDF   Save Metformin is a first-line treatment for type 2 diabetes (T2D) due to its excellent blood glucose-lowering effects, safety, and multiple metabolic benefits. However, significant side effects related to dosages are also observed in clinical practice. Therefore, reducing the dosage while achieving satisfactory glycemic control becomes a crucial step in optimizing clinical treatment. Recent studies have shown that static magnetic fields (SMFs) have certain ameliorative effects on diabetic mice, but the combinational effects of SMFs and hypoglycemic agents are still unknown. In this study, the effects on high-fat diet and streptozotocin-induced T2D mice by combining a downward, moderate-intensity SMF with metformin were investigated. The fasting blood glucose, glucose tolerance, GLP-1 levels, and insulin secretion were all assessed. The results revealed that although the antihyperglycemic effect of SMF combined with lower-dose metformin (150 mg/kg) was not greater than that of higher-dose metformin (250 mg/kg), it was superior to SMF or low-dose metformin alone. Furthermore, 16S rRNA gene sequencing analysis of intestinal contents revealed that the abundance ofRuminococcusandAdlercreutzia, which were positively related to diabetes, were significantly decreased by the combinational treatment of SMF and metformin. The abundance of probiotics such asLactobacillusandOscillospirawere also increased after SMF exposure. Therefore, this study showed that this type of SMF could improve the intestinal microflora of diabetic mice and enhance the hypoglycemic effect of low-dose metformin, which could potentially provide a new feasible, safe and effective combination strategy for the treatment of diabetes Related Articles | Metrics

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Phosphorylation of the mitochondrial antiviral signaling protein MAVS regulates its antiviral activity WANG Tingting, XU Changzhi, ZHU Lin, LIU Xuan 2025, 42 (2):  24.  doi: 10.3969/j.issn.2095-1736.2025.02.024 Abstract ( 34 )   PDF   Save Mitochondrial antiviral signaling protein (MAVS) is an important adaptor protein in the host innate immunity pathway in response to RNA viruses, playing important regulatory roles in innate immune and inflammatory responses. The expression and the function of MAVS are regulated by two mechanisms, namely transcription and post-translational modification, and phosphorylation and ubiquitination play important roles in regulating the transport, function and stability of MAVS. In order to further investigate the effect of MAVS phosphorylation on the levels of IFN-β and TNF-α production in cells, seven previously unreported MAVS phosphorylation sites, including pS193, pS226, pS238, pS246, pS249, pT252, and pT370, were identified by tandem mass spectrometry. Subsequently, the above phosphorylation sites were mutated into alanine (A) to construct a phosphorylation-deficient mutant. It was found that MAVS T370A mutation could significantly inhibit the transcriptional activity of IFN-β and NF-κB reporter genes downstream of the MAVS signaling pathway, and then down-regulate the expression of IFN-β and TNF-α, and inhibit the antiviral response of cells. These results suggested that T370, as an unreported phosphorylation site of MAVS, played an important role in the host innate immune signaling pathway. Further elucidation of kinases mediating phosphorylation at T370 will be of great significance for revealing the molecular mechanism of innate immune homeostasis maintenance and host antiviral activity. Related Articles | Metrics

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Screening of bacteriocin-producing lactic acid bacteria Lpe59 and mining of bacteriocin gene cluster ZHANG Zongyuan, YUE Dandan, ZHOU Liuzhu, WANG Xueyan, CHEN Denghui, PAN Mengshi, XU Hongguang, LI Guanjie, YIN Wenbing 2025, 42 (2):  33.  doi: 10.3969/j.issn.2095-1736.2025.02.033 Abstract ( 27 )   PDF   Save In order to screen a bacteriocin-producing lactic acid bacteria and explore its bacteriocin gene cluster, the pathogensEscherichia coliandStaphylococcus aureuswere used as indicator, through initial and repeated screenings using conventional methods,Lactobacillus pentosusLpe59, a strain with strong antibacterial activity and bacteriocin production was isolated. The whole-genome information of strain Lpe59 was obtainde through high-throughput sequencing, the results showed the genome size of strain Lpe59 was 3274558 bp, encoding 3047 genes, with 2434, 2203, 1614, 107 and 4 functional genes annotated in COG, GO, KEGG, CAZY and VFDB databases, respectively. Using the online software AntiSMASH 6.11, four types of secondary metabolite gene clusters were predicted in strain Lpe59, including ribosomally synthesized and post-translationally modified peptide-like (RiPP-like) compounds, type Ⅲ polyketide synthase (T3PKS) metabolites, terpene, and cyclic-lactone-autoinducer. Additionally, a gene cluster for the synthesis of class Ⅱ bacteriocins Plantaricin K and Plantaricin EF was predicted through Bagel4. In conclusion, antibacterial tests and genomic predictions suggested thatL.pentosusLpe59 was a promising lactic acid bacterium with biocontrol potential and the ability to produce bacteriocins. The research findings provided a theoretical basis for the further development and application of its bacteriocins, Plantaricin K and Plantaricin EF. Related Articles | Metrics

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The mechanism of apoptosis of gonadal cells induced by metformin combined with chemoradiotherapy and inhibition of vulva tumor in nematodes HAN Tianyi, ZHAO Xipeng, ZHAO Guoping, WANG Ruru 2025, 42 (2):  40.  doi: 10.3969/j.issn.2095-1736.2025.02.040 Abstract ( 26 )   PDF   Save To explore the mechanism of metformin combined with radiotherapy and chemotherapy-induced apoptosis inCaenorhabditis elegansgerm cells, wild-type and mutant strains with defects inegl-1, ced-3, ced-4, hus-1,andcep-1genes were selected. Fluorescence staining was used to detect the apoptosis levels of germ cells in wild-type and mutant worms after treatment with metformin combined with 50 Gy irradiation or cisplatin. qPCR was used to measure the expression levels of genes related to defects, and the development of vulval tumors inlet-60worms was observed under a microscope. 1 mmol/L metformin increased the apoptosis of germ cells induced by radiation and 100 μmol/L cisplatin in wild-type worms. Mechanistically, core apoptosis genesegl-1, ced-4, ced-3,and DNA damage response geneshus-1, cep-1mediated the apoptosis signals induced by metformin in combination with radiation and cisplatin. Additionally, metformin combined with cisplatin effectively inhibited the development of vulval tumors inlet-60worms. These results indicated that metformin increased apoptosis of germ cells induced by radiotherapy and chemotherapy, and DNA damage response pathways and core apoptosis pathways were involved in the process of metformin-regulated sensitivity to radiotherapy and chemotherapy. Related Articles | Metrics

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Application of ion beam implantation technology to improve the vitamin K2 synthesis capacity in Lactobacillus MU Jingwei, YANG Xu, WANG Hongbo, WANG Li, SUN Manman, WANG Peng 2025, 42 (2):  46.  doi: 10.3969/j.issn.2095-1736.2025.02.046 Abstract ( 24 )   PDF   Save Vitamin K2 (VK2) plays a essential role in maintaining human bone and cardiovascular health. However, due to the silence or non-expression of some genes in the VK2 synthesis pathway of lactic acid bacteria, VK2 is negligible in dairy products. In this study, using Lactobacillus fermentum (LF1) as the starting strain, the lethality and the positive and negative mutation rates were analyzed under different ion beam injection conditions. The optimal parameters were determined to be an energy of 10 keV and a dose of 75(×2.6×1013 N+/cm2). Through successive ion beam iterations coupled with adaptive laboratory evolution (ALE), the high-yield VK2 strain LFX was obtained, achieving a production rate of 0.67 mg/g. The strain’s genetic stability was confirmed through serial passage experiments. Transcriptome analysis revealed that in the VK2-related synthesis pathway of LFX, genes associated with the biosynthesis of the naphthoquinone core and the polyprenyl side chain were markedly upregulated. Moreover, pathways involved in the antioxidant defense, cofactor biosynthesis, and central carbon and nitrogen metabolism were substantially enhanced. The findings suggested that iterative ion beam implantation and ALE could synergistically optimize metabolic fluxes, enhancing VK2 biosynthesis and stability in the developed strains. Related Articles | Metrics

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Construction of homologous recombinant vectors and natural transformation of Arthrospira WANG Kang, CUI Hongli, LI Wenjun, QIN Song 2025, 42 (2):  52.  doi: 10.3969/j.issn.2095-1736.2025.02.052 Abstract ( 26 )   PDF   Save Arthrospirais a multicellular filamentous cyanobacterium with high economic value. However, genetic manipulation ofArthrospirahas been a major challenge, especially the lack of an easy-to-operate genetic transformation technology. To further develop the potential ofArthrospirafor genetic engineering applications, this study attempted to screen strains with natural transformation ability from 12 differentArthrospiraand evaluated the impact of homologous flanking sequence and insertion fragment lengths on natural transformation efficiency. The results showed that only strains S17 and S39 could be genetically modified through natural transformation. Both homologous flanking sequences and insertion DNA fragment lengths influenced transformation efficiency. It was found that increasing the homologous flanking sequences to over 1 500 bp could significantly increase the positive rate. Conversely, the length of the inserted DNA fragment was negatively correlated with the positive rate, so it was necessary to consider increasing the length of homologous flanking sequences when using longer inserted DNA fragments. In addition, this study also confirmed that the homogeneity ofArthrospirawas related to the duration of continuous screening, where after 120 days of screening, homogenous strains accounted for over 50% of the positive strains; these homogenous strains remained genetically stable for at least 6 months. It was expected that this work would provide technical support for the development ofArthrospiraas a bioreactor for producing high-value compounds. Related Articles | Metrics

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Identification of the FERONIA gene family in rubber trees and expression analysis in response to stress from Colletotrichum gloeosporioides YUE Liujun, AN Bang 2025, 42 (2):  59.  doi: 10.3969/j.issn.2095-1736.2025.02.059 Abstract ( 28 )   PDF   Save To further study the function of FERONIA (HbFER) inHevea brasiliensis, the paper used bioinformatics methods to screen and identify 31HbFERfamily members from theH. brasiliensisgenome, and conducted detailed analysis of their physicochemical properties, gene sequences, domains, and chromosome location. The results showed that these 31 HbFERs were unevenly distributed on the nine chromosomes ofH. brasiliensis, encoding proteins ranging from 453 to 900 amino acids in size. Sequence analysis revealed that all HbFER proteins contained Malectin-like domains, and most of them also contained PKc-like domains. Through intra-species and inter-species collinearity analysis, it was found that there were three pairs of collinear genes forHbFERinH. brasiliensis, and the gene collinearity relationship betweenH. brasiliensisand rice was relatively significant, indicating that FER had undergone different evolutionary processes among different groups and species, and the gene functions have differentiated. Expression profile analysis revealed that the expression levels of most HbFERs inH. brasiliensisleaves decreased to varying degrees when infected withColletotrichum gloeosporioides, while the expression levels of individual genes increased. This indicated that different genes in theHbFERfamily ofH. brasiliensismight play differentiated roles in response to pathogen infection. In summary, our results provided a certain reference for further studying the role of HbFER in disease resistance inH. brasiliensis, and laid a foundation for molecular breeding ofH. brasiliensisfor disease. Related Articles | Metrics

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Identification and characterization of XO-binding natural products from sunflower calathide YAN Haoqian, ZHANG Yang, XING Fangyu, GAO Jianping, ZHANG Tianyang, LI Xiaojing, SONG Mowei, SUN Bingbing, MA Li, ZHANG Guifeng 2025, 42 (2):  66.  doi: 10.3969/j.issn.2095-1736.2025.02.066 Abstract ( 35 )   PDF   Save Natural products with inhibition of xanthine oxidase (XO) activity were screened from sunflower calathide. XO was used as a carrier to specifically adsorb and isolate candidate compounds from sunflower calathide extracts, and they were identified by high-resolution liquid mass spectrometry (LC-MS) to study the inhibitory activity and inhibition mechanism of possible compounds on XO. Thirteen compounds were isolated from the ethanol extract of sunflower calathide, including cafestol (IC50=47.49 μg/mL,P<0.0001), which exhibited competitive and reversible inhibition against XO. Circular dichroism with molecular docking verified that cafestol could bind to XO and interact with it and reduce its activity. The binding of cafestol to the FAD region of the enzyme hindered the electron transfer during the substrate oxidation reaction, making the structure of the enzyme more dense, and the substrate was difficult to locate into the active cavity, resulting in a decrease in its catalytic activity. The quenching mechanism of cafestol against xanthine oxidase belonged to static quenching, and the main driving force was hydrophobic, and cafestol had only a single or one binding site on the XO active cavity. The results showed that cafestol was a potential ingredient that inhibited XO activity, which provided a scientific basis for the screening of therapeutic drugs such as hyperuricemia. Related Articles | Metrics

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Structural characteristics and influencing factors of zooplankton community in the Xuancheng section of the Shuiyang River ZHENG Zhixia , ZHU Xiaogang , YE Bibi, YUAN Zhen, YANG Wentao , LIU Yannian 2025, 42 (2):  75.  doi: 10.3969/j.issn.2095-1736.2025.02.075 Abstract ( 36 )   PDF   Save To elucidate the spatiotemporal distribution characteristics and water environmental influencing factors of zooplankton community structure in the Xuancheng section of the Shuiyang River, non-metric multidimensional scaling (NMDS) was applied to analyze the succession law of zooplankton communities and redundancy analysis (RDA) was used to investigate the correlation between zooplankton distribution and water environmental factors. Zooplankton surveys were conducted at 12 sampling points in the Xuancheng section of the Shuiyang River in August and November 2022, March and June 2023, respectively. The results showed that a total of 151 species of zooplankton in 4 categories were detected, mainly rotifera. The number of species was the highest in June and the lowest in March, and the number of species in the middle and lower reaches of the river was higher than that in the upper reaches. There were 16 dominant species, mainly Protozoa and Rotifera, and there were multiple indicator species of eutrophication in water bodies. There were temporal and spatial differences in zooplankton stock and diversity indexH′, JandD. The structure of zooplankton community showed certain differences and consistency in time and space. In August, zooplankton were greatly affected by NO－3-N and TN, NO－3-N, NH+4-N and DO in November, WT in March, COD, NO－3-N, pH and PO3－4-P in June. On the whole, the water body in the study area has been eutrophicated, which is α —medium pollution. Related Articles | Metrics

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Collagen degradation in the tail of Xenopus laevis during metamorphosis LU Hui, ZHANG Yang, GAO Jianping, XING Fangyu, ZHANG Tianyang, LUO Xi, ZHANG Guifeng 2025, 42 (2):  84.  doi: 10.3969/j.issn.2095-1736.2025.02.084 Abstract ( 28 )   PDF   Save Biological mass spectrometry was employed to investigate the identification of various collagen types and their degradation processes during the tail degeneration ofXenopus laevistadpole metamorphosis development. Tail samples were collected at NF 61－65. The collagen types were identified using high-performance liquid chromatography coupled with tandem mass spectrometer (HPLC-MS), and quantitatively analyzed based on marker peptides. Mass spectrometry analysis revealed the presence of collagen types Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ, and Ⅻ in the enzymatic digestion products ofXenopus laevistail samples. Collagen type Ⅰ marker peptide GVLGPQGAR was chosen as an external standard for quantification to investigate changes in its content during different growth stages. Similarly, dynamic changes in collagen content for types Ⅲ, Ⅵ and Ⅻ were also analyzed using the same method. The results demonstrated that each type of collagen exhibited a faster degradation rate during NF 61－63 stage accompanied by tail degeneration; however, in NF 64－65 stage when tail atrophy and degeneration ceased, the degradation rate slowed down significantly. Furthermore, the study investigated matrix metallo proteinase (MMPs) types present in tail samples at different stages, and preliminarily explored the relationship between dynamic changes in diameter of tail collagen fibers. Related Articles | Metrics

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Microbial mediated anaerobic ammonia oxidation coupled with different electron acceptors WANG Furong, WEI Manman 2025, 42 (2):  91.  doi: 10.3969/j.issn.2095-1736.2025.02.091 Abstract ( 42 )   PDF   Save Anaerobic ammonia oxidation coupled with different electron acceptors is a series of novel biological denitrification approaches reported in recent years, which has not only attracted much attention in the field of wastewater biological treatment, but also played a crucial role in geochemical cycles of nitrogen, sulfur, iron, and manganese. This paper focused on four anaerobic ammonia oxidation processes coupled with different electron acceptors, including nitrite-dependent anaerobic ammonia oxidation, sulfate type anaerobic ammonia oxidation (Sulfammox), anaerobic ammonium oxidation coupled to Fe(Ⅲ) reduction (Feammox) and anaerobic ammonium oxidation mediated by MnO2(Mnammox). The reaction mechanisms and reaction equations were summarized and the phylogenetic diversity and metabolic characteristics of different anaerobic ammonia oxidizing bacteria were reviewed. The research progress and functional microorganisms of anaerobic ammonia oxidation processes coupled with different electron acceptors were elucidated. Finally, the application bottlenecks and the future development were discussed. The aim was to provide theoretical support for further in-depth research, and to provide a new perspective for promoting the development of anaerobic ammonia oxidation technology with different electron acceptors and the application in the field of wastewater denitrification. Related Articles | Metrics

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Progress on hyaluronic acid in treatment of osteoarthritis HAN Wenying, RUAN Haihua 2025, 42 (2):  99.  doi: 10.3969/j.issn.2095-1736.2025.02.099 Abstract ( 36 )   PDF   Save The effects of hyaluronic acid of different molecular weights on inflammatory pathways and the cellular activities of hyaluronic acid after specific binding to different receptors were systematically described. Mechanistic studies of hyaluronic acid of different molecular weights in the treatment of osteoarthritis and its clinical studies were also reviewed, mainly analyzing the role of hyaluronic acid in the treatment of osteoarthritis in maintaining intra-articular physicochemical properties, regulating inflammatory signaling pathways and protecting articular cartilage, and pointing out the shortcomings of intra-articular injection of hyaluronic acid in the treatment of osteoarthritis. This paper introduced the anti-inflammatory, pro-inflammatory and cartilage-protective effects of hyaluronic acid from the perspective of the treatment of osteoarthritis, with a view to providing a reference for the relevant research on hyaluronic acid treatment of osteoarthritis. Related Articles | Metrics

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Rapid detection of five mouse pathogens by multicolor melting curve analysis XU Yimeng, LIU Xinyi, HU Shanshan, ZHOU Yuxun, LI Kai, XIAO Junhua 2025, 42 (2):  107.  doi: 10.3969/j.issn.2095-1736.2025.02.107 Abstract ( 16 )   PDF   Save Based on multicolor melting curve analysis, an efficient and low-cost simultaneous detection protocol of five mouse pathogens for the rapid detection of infection in experimental animals (Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, Pasteurella pneumotropica,andProteus) was established. Meanwhile, the capsicum genome was added as an internal reference to exclude false negatives and make the results more accurate and reliable. By designing the probes, the melting peaks of different pathogens appeared at different Tm values in different fluorescence channels: in the FAM fluorescence channel, the melting peak Tm values ofS. aureusandK. pneumoniaewere in the range of （53.51±0.94） ℃ and （61.23±0.34） ℃, respectively; in the ROX channel, the melting peaks ofP. aeruginosaandP. pneumotropicawere in the range of （61.70±0.66） ℃, （55.98±0.10） ℃; in CY5 channel, the melting peak Tm values ofProteusandCapsicum annuumL. were at （63.91±0.19） ℃, （57.43±0.31） ℃, respectively. The method was verified to have different detection limits for different pathogens with 102copies forS. aureus, 101copies forK. pneumoniae, 102copies forP. aeruginosa, 102copies forP. pneumotropica, and 102copies forProteus. In summary, the multicolor melting curve method established in this work was capable of rapid and effective detection of a wide range of common mouse pathogens in a single tube. Related Articles | Metrics

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Exploration on the teaching model of scenario-based experimental course for cellular and molecular basis QIAN Meirui, MIAO Jinlin, HUANG Wan, CHEN Zhinan, JIANG Jianli 2025, 42 (2):  112.  doi: 10.3969/j.issn.2095-1736.2025.02.112 Abstract ( 27 )   PDF   Save The purpose of experimental course for the integrated course of cellular and molecular basis was to improve students’ practical ability to integrate and flexibly apply the key and difficult points they have learned, and to cultivate their innovative scientific research thinking in proposing and solving problems. This study analyzed the teaching status of this course, including problems such as rigid teaching methods, redundant and poor continuity of teaching content, outdated experimental teaching content, and a single method for evaluating teaching effect. The reform practices of experimental courses was explored from three aspects: course design, experimental content, and objectives of course assessment. Based on clinical cases-oriented teaching model to cultivate innovation and entrepreneurship abilities among college students in the new era, a “scenario-based” experimental class was designed. The experimental teaching content was also reconstructed based on the training mode of cultivating scientific research thinking. The establishment of a multi-dimensional assessment method based on scientific thinking and supplemented by experimental skills improved the effectiveness and innovation of teaching design, promoted the course construction of cellular and molecular basis, and effectively cultivated students’ innovative scientific research thinking and research literacy. This study provided new ideas and practical basis for cultivating medical talents with scientific innovation spirit and practical ability. Related Articles | Metrics

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Construction of project-based teaching model and the practice of plant taxonomy from normal universities ZHANG Yu, LI Ziyi, YI Su, CHEN Yuanqi 2025, 42 (2):  118.  doi: 10.3969/j.issn.2095-1736.2025.02.118 Abstract ( 30 )   PDF   Save 在师范专业认证背景下，结合项目式教学策略，构建以“学生自主实践，教师指导促学”为理念的植物分类学课程教学模式，并开展教学实践。该课程的项目式教学分为：准备阶段，学生基于学习目标自主选择项目；实施阶段，组内成员分工完成实践报告；成果展示阶段，视频展示并组间互评。阐述该课程项目式教学模式的构建、实践过程和教学效果等，在帮助学生掌握植物分类知识和提高师范技能方面取得良好成效，为师范类生物科学专业课程改革提供参考范例和有益启示。 Related Articles | Metrics

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Curriculum reform and practice in bioinformatics according to outcome-based education concept ZHAO Fan, SONG Yongbo, WANG Miao, ZHAO Yongshan, WANG Lin, ZHANG Jinghai 2025, 42 (2):  121.  doi: 10.3969/j.issn.2095-1736.2025.02.121 Abstract ( 23 )   PDF   Save In this study, according to the concept of outcome-based education, it proposed a progressive reform strategy focusing on learning ability, practical skills, and engineering thinking at three levels: theoretical learning, technological application, and interdisciplinary application, along with implementation and evaluation methods. The aim of this strategy was to progressively cultivate students’ ability to use the bioinformatics techniques theoretically, flexibly and systematically. The reform measures proposed in this study were expected to strengthen the cultivation and output of the professionals with diverse backgrounds and industry demands, facilitating the narrowing of the gap between the student cultivation in colleges and universities and the requirements of the bio-pharmaceutical industries. Related Articles | Metrics

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Enhancing genetics experiment teaching quality through competition WEI Yuan, WANG Honggang, ZHU Yushan, LI Yanjun, LI Dengwen, ZHAO Nianxi 2025, 42 (2):  127.  doi: 10.3969/j.issn.2095-1736.2025.02.127 Abstract ( 22 )   PDF   Save The basic experimental skills competition focuses on assessing students’ proficiency in basic experimental projects and their comprehensive application of knowledge, serving as a dual evaluation of the quality of experimental teaching and students’ practical abilities. Using the “Preparation and observation of polytene chromosome of larvae ofChironomidae” project from the Life Science Competition for Tianjin College Students as a starting point, our course team integrated contestant selection and training with experimental teaching, leveraging student initiative to expand class time, reinforce fundamental and detailed training, and cultivate students’ ability to identify and solve problems at a deeper level. Questionnaires were distributed to participants to assess competition outcomes, which were then analyzed alongside results from the experimental courses. The findings indicated that competitive experimentation could enhance learning through active skill development, consolidation of foundational knowledge, and improvement in the quality of experimental teaching. Related Articles | Metrics