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Comparative genomics study of the typical bacteria Acidiphilium in biohydrometallurgy system
LI Xing, LOU Wei, LI Liangzhi, YIN Huaqun, XU Xizhong, XIA Ling, MENG Delong
2025, 42 (1):
48.
doi: 10.3969/j.issn.2095-1736.2025.01.048
Acid mine drainage (AMD)
represents a uniquely acidic ecosystem, marked by oligotrophic
conditions, a low pH environment, and elevated concentrations of heavy
metals. Within this milieu, a diverse array of acidophilic and
acid-tolerant microorganisms thrives, playing pivotal roles in the
biogeochemical cycling of carbon, nitrogen, sulfur, and various other
elements. The complex interplay between heterotrophic and autotrophic
microorganisms underscores a significant ecological niche, with theAcidiphiliumgenus, a group of facultative heterotrophic microorganisms constituting
a minor proportion (<1%) of the AMD microbial community. Notably,
these organisms often engage in symbiotic interactions with prevalent
species likeAcidibacillus, suggesting thatAcidiphiliumspecies fulfill unique and crucial ecological functions within the AMD
ecosystem. In order to investigate the ecological function ofAcidiphilium, a strain ofAcidiphiliumwas isolated from an extremely acidic environment. It was identified asAcidiphilium cryptumAcc2 after being compared with ANI by 16S rDNA. TheAcidiphilium cryptumAcc2 genome contained 1066 protein-coding genes with a total sequence
length of 4917178 bp, all of which were larger than the gene size of
other strains in the genus. It was an open genome, containing 47242
protein-coding genes, 699 protein-coding genes (1.48%) were identified
as core genes, 33457 protein-coding genes were variable genes (70.82%),
and 2705 protein-coding genes were unique genes (5.73%). The Ka/Ks
values of heavy metal resistance genes of 95.5% were all lower than 1,
which showed strong purification selection. The flow and exchange
frequency between genes in the genome of the genusAcidiphiliumwas higher than that of other species, and it had a greater positive
impact on the evolution and environmental adaptability of strains. This
research shed light on their evolutionary trajectories and gene transfer
mechanisms, offering insights into their environmental adaptation
mechanism and ecological significance in AMD environments.
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