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Anti-inflammatory effect and mechanism of Pseurata H on inflammation model of LPS-induced RAW264.7 cells
YANG Dongjuan, ZHA Guangcai, ZOU Xianghui, WU Dantong, WU Qingting
2024, 41 (2):
39.
doi: 10.3969/j.issn.2095-1736.2024.02.039
To study the
anti-inflammatory effect and mechanism of Pseurata H on peritoneal
macrophages (RAW264.7 cells) inflammation models, RAW264.7 cells were
stimulated with LPS to establish an inflammation model in vitro, and
Pseurata H with the concentrations of 10, 20, and 40 μmol/L was used to
interfere with this cell model. The cytotoxicity of Pseurata H in
RAW264.7 cells was determined by CCK-8 colorimetric assay. Griess assay
was employed to detect the content of NO in the supernatant. The release
of IL-6 and TNF-α in the supernatant was assayed using ELISA. The
protein expressions of COX-2, iNOS, p-NF-κB and the p-ERK were measured
by Western Blot. The mRNA expressions of iNOS, TNF-α, IL-6 and IL-1β
were analyzed by RT-qPCR. The results showed that Pseurata H (10, 20, 40
μmol/L) displayed no apparent cytotoxicity in RAW264.7 cells. The
release levels of NO, iNOS, TNF-α, and IL-6 were decreased by Pseurata H
(10, 20, 40 μmol/L). In addition, Pseurata H also inhibited the
expressions of iNOS, COX-2, NF-κB, p-NF-κB, ERK, and p-ERK proteins with
concentration effect. Pseurata H has a significant anti-inflammatory
effect, and its mechanism of action may be through the inhibition of
NF-κB/MAPK signaling pathway to exert anti-inflammatory effects.
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