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18 December 2024, Volume 41 Issue 6 Previous Issue   
Space microbiology and its bioengineering applications
SUN Lichao, ZHAO Pengzhuo, HU Wei, HUO Yixin
2024, 41 (6):  1.  doi: 10.3969/j.issn.2095-1736.2024.06.001
Abstract ( 149 )   PDF  
Investigating the behavior and response mechanisms of microorganisms in space environments or simulated space environments aids in understanding their adaptability and evolutionary principles in extreme conditions. In this study, the impacts of microorganisms on the space environment were analyzed, and the techniques and equipments for studying space microbiology were introduced. Molecular mechanisms underlying microbial growth and morphology, mass transfer, secondary metabolite production, immune characteristics, biofilm formation, DNA damage and stress responses under space microgravity and radiation were thoroughly reviewed. The potential of space microbiology in aerospace breeding and biomedical engineering were further prospected. This work holds significant implications for space breeding, provides scientific guidance for space missions, and advances the development of manned spaceflight and space exploration.
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Identification and analysis of differential expression genes regulated by UHRF1 in breast cancer cells #br#
GONG Chunxue, DONG Qincai, LIU Xuan, CAO Cheng
2024, 41 (6):  12.  doi: 10.3969/j.issn.2095-1736.2024.06.012
Abstract ( 153 )   PDF  
Ubiquitin-like with PHD and Ring finger domains 1 (UHRF1) is an epigenetic regulator that maintains DNA methylation modification and plays critical roles in embryonic development, tumor progress and prognosis. In this study, the differential expression genes regulated by UHRF1 was identified, and the associated cell functions, metabolic pathways, and related diseases these genes involved were investigated. Total RNA extracted from UHRF1 knockdown cells (MCF-7/shRNA-UHRF1) or scramble cells (MCF-7/shRNA-Scramble) as a control was subjected to RNA-Seq analysis, then the differential expression genes were screened by using DESeq2 software and partially validated by qRT-PCR. Gene ontology (GO), Kyoto Encylopedia of Genes and Genomes (KEGG) and Disease ontology (DO) analyses were employed to investigate the potential biological functions of these genes. The results showed that a total of 2926 genes expression were regulated by UHRF1. Among them, 1453 genes were down-regulated and 1473 genes were up-regulated by UHRF1 knockdown. GO and KEGG analysis showed that these genes were primarily enriched in cell metabolism, immune regulation, signal transduction, cardiovascular disease and tumorigenesis. DO analysis showed that they were mainly involved in cancer and neurological diseases. These results suggested that DNA methylation mediated by the epigenetic regulatory factor UHRF1 can regulate the differential expression of genes at the transcriptional level, providing data support for the participation of UHRF1 in the regulation of relevant signaling pathways.
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Inhibition of glutamine synthetase in glioma cells enhances radiotherapy-induced ferroptosis #br#
LU Yijun, ZHOU Chen, QIAN Junchao
2024, 41 (6):  20.  doi: 10.3969/j.issn.2095-1736.2024.06.020
Abstract ( 110 )   PDF  
The study aims to investigate the role of glutamine synthetase (GS) in radiotherapy-induced ferroptosis in glioma. The glioma cells GL261 were treated using Ionizing radiation (IR) and GS inhibitor (L-methionine sulfoximine, MSO). The levels of reactive oxygen species (ROS) was detected with the DCFH-DA fluorescent probe. The changes in glutathione (GSH) content were measured using a GSH assay kit. Cellular lipid peroxidation levels were investigated with the C11-BODIPY 581/591 fluorescent probe. The expression of the ferroptosis marker PTGS2 was detected by RT-qPCR. Cell proliferation rate and cell viability were assessed using CCK-8 assay. The effects of MSO and RT on growth inhibition of tumors were further investigate by establishing murine orthotopic glioma model. The results showed that inhibiting the activity of GS promoted the production of ROS and GSH depletion in cells after radiotherapy, enhanced lipid peroxidation, up-regulated the expression of PTGS2, and significantly inhibited cell proliferation and viability. Animal experiments confirmed that the combination of MSO and radiotherapy showed a stronger tumor suppression effect compared to conventional radiotherapy. In conclusion, inhibiting glutamine synthetase can significantly enhance the induction of ferroptosis in glioma cells by radiotherapy. This approach increases the sensitivity of tumor cells to radiotherapy and provides new strategies and targets for clinical treatment.
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Study on the anti-inflammatory effect and mechanism of catechin on mouse alveolar macrophages
CHEN Shuzhen, XU Qiufeng, WANG Huangbin, HUANG Shasha, HUANG Liduan, XIONG Min
2024, 41 (6):  26.  doi: 10.3969/j.issn.2095-1736.2024.06.026
Abstract ( 97 )   PDF  
The purpose of this study was to explore and compare the anti-inflammatory effects and the possible mechanisms of catechin and epicatechin in mouse alveolar macrophages(MH-S). Lipopolysaccharide(LPS)was used to stimulate MH-S cells to establish a cellular inflammation model. The effects of catechin and epicatechin on MH-S cell viability were detected by MTS and LDH assays. The contents of interleukin-1β(IL-1β), IL-6, and TNF-α in the supernatant were detected by enzyme-linked immunosorbent assay(ELISA). Real-time quantitative polymerase chain reaction(qRT-PCR) was used to detect the mRNA transcription levels ofNos2,Il-6, Tnf-α,Grp78, Ire1α, Perk, andAtf6. The protein expression of iNOS was detected by Western Blot. The results showed that 6.25-100 μg/mL of catechin and epicatechin had no significant toxicity on MH-S cells. Both catechin and epicatechin down-regulated the secretion of IL-1β and the protein expression of iNOS in LPS-induced MH-S cells in a concentration-dependent manner. Meanwhile, catechin and epicatechin reduced the mRNA transcription levels of iNOS, IL-6 and endoplasmic reticulum stress(ERS)-related protein IRE1α. The anti-inflammatory activity of catechin was stronger than that of epicatechin at low concentrations, but catechin was lower than epicatechin at high concentrations. These results indicated that catechin and epicatechin had anti-inflammatory effects by reducing the secretion of IL-1β, IL-6 and the expression of iNOS, which might be related to the inhibition of ERS.
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Antibiotic-resistant genes dissemination in the intestinal flora of Canorhabditis elegans in response to antibiotic pressure#br#
DA Yanmei, ZHANG Ying, LI Shunshun, GAO Zeping, HUANG Tao, SUN Qingye, ZHOU Guowei
2024, 41 (6):  31.  doi: 10.3969/j.issn.2095-1736.2024.06.031
Abstract ( 91 )   PDF  
Cryptobacterium hidradiiused as a model organism, qPCR was combined with plate screening methods to demonstrate that the antibiotic-resistant genes (ARGs) donor bacteriumEscherichia coliMG1655 and the acceptor bacteriumSalmonella typhimuriumwere both able to colonize stably in the intestine. The ratio of the number of antibiotic-resistantS.typhimuriumobtained via conjugation to the number of total intestinal bacteria was used to characterize the diffusion efficiency of ARGs in the intestine. The ARG diffusion efficiency increased and then decreased with increasing tetracycline concentrations (0, 0.08, 0.4, 2, 10, and 50 mmol/L) over three days of incubation, and it peaked at 0.78% and 1.96% on the second and third days in the 0.4 mmol/L tetracycline-treated group, respectively. Moreover, the relative abundance of conjugation-relevant functional genestrfA/16S rRNAandtrbB/16S rRNAas well as activities of antioxidant enzyme in intestinal tissues shared similar trends with the ARG diffusion ratios in the gut of snails. Therefore, the present study demonstrated that a brief and low concentration of antibiotics could promote the diffusion of ARGs in the animal intestine.
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Expression and fermentation optimization of recombinant sucrose isomerase in Bacillus subtilis
JIANG Chen, WU Xintong, CHEN Huiling, LIU Le, ZHANG Qian, LI Xianzhen, GUO Xiaoyu
2024, 41 (6):  39.  doi: 10.3969/j.issn.2095-1736.2024.06.039
Abstract ( 83 )   PDF  
Through promoter engineering and medium optimization strategies, sucrose isomerase with high expression level regulated by non-inducible promoter was constructed, and the effects of different promoters on sucrose isomerase expression of recombinant strain Bacillus subtilis SCK6 were investigated. Unrestricted cloning method was used to construct 6 non-inducible single promoters (PHpal Ⅰ, P43, PgsiB, PsrfA, PamyQ and PaprE) and 3 double promoters (P43-PgsiB, 2P43 and 2PgsiB) regulated sucrose isomerase recombinant strains, respectively. The results showed that the activity of sucrose isomerase under the control of 2P43 was the highest (19.08±0.09) U/mL, which was 1.37 times of that under the control of the original promoter Pgrac. The expression level of sucrose isomerase was further improved by optimizing the medium. The fermentation medium of recombinant strain regulated by 2P43 was optimized from carbon source, nitrogen source and inorganic salt by single factor method. The optimal fermentation medium formula was 25 g/L glycerol, 30 g/L yeast extract powder and 20.75 g/L soybean peptone. The activity of Pal Ⅰ enzyme was increased to (80.36±9.32) U/mL. It was 4.2 times of the original medium (19.08±0.09) U/mL. In this experiment, the expression system of double-promoter regulated Bacillus subtilis sucrose isomerase with high product activity was successfully constructed, which laid a theoretical foundation for subsequent research and production of sucrose isomerase available for food, and provided a theoretical basis for industrial production and application of Isomaltulose.
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Analysis of microbial diversity in the roots of terrestrial orchids in Yunnan Province
WU Fengjinglin, CHEN Jianxin, YANG Yalin, JI Jingjie, ZHANG Donghua, MA Huancheng, WU Jianrong
2024, 41 (6):  47.  doi: 10.3969/j.issn.2095-1736.2024.06.047
Abstract ( 71 )   PDF  
In order to investigate the distribution of microorganisms in the roots of terrestrial orchids under different growth conditions, the differences of microbial communities in the roots under different growth conditions were analyzed by metagenomic sequencing technology, and the potential functions of these microorganisms were predicted. The results showed that the abundance and diversity of microorganisms in the roots of wild orchids were higher than that of those ochids in the cultivated condition. The main groups of microorganisms in the roots of orchids in the wild condition were Proteobacteria, Actinobacteria, and Ascomycota, while those in the cultivated condition were Proteobacteria, Basidiomycota and Viruses noname. In addition, there were significant differences in the main dominant taxa of orchid mycorrhiza fungi (OMF)in the wild and in the cultivated condition. The relative abundance of arbuscular mycorrhizal fungi (AMF) was significantly higher in the cultivation than in the wild. By predicting the metabolic functions of microorganisms in the roots under two growth conditions, it was found that functional genes for carbohydrate metabolism, amino acid metabolism, energy metabolism and metabolism of cofactors and vitamins were more abundant in the wild condition. The results of this study provided a theoretical basis for the conservation, artificial mycorrhizalization seedling cultivation, and pest control of terrestrial orchids.
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Physiological responses of Melica transsilvanica to endophytic fungi infection under conditions of water stress
ZHANG Mengmeng, WANG Chuanzhe, HE Jiakun, SHI Chong
2024, 41 (6):  56.  doi: 10.3969/j.issn.2095-1736.2024.06.056
Abstract ( 72 )   PDF  
This study explored the impact of water stress on the physiological responses ofMelica transsilvanicato endophytic fungal infection. Using both endophyte-infected (E+) and non-infected (E-) plants, a controlled water experiment with soil moisture levels of 60%-65% field water capacity (FWC) was set up for the control (CK), 85%-90% FWC for the high humidity treatment (W), and 15%-20% FWC for the drought treatment (D). The significant differences were found in proline content and superoxide dismutase (SOD) activity between E+ and E- plants following 7 days of both high humidity and drought stress (P<0.05). After 14 days of stress, the significant differences were observed in chlorophyll, proline, malondialdehyde (MDA) content, SOD, peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT) activities in E+ and E- plants under the same conditions (P<0.05). After 21 days of stress, E+ plants had significantly increased MDA content and relative cell membrane permeability compared to E- plants under both conditions (P<0.05). The results suggested that endophytic fungal infection enhanced the tolerance ofMelica transsilvanicato water stress.
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Metabolomic analysis of postharvest blueberry fruits treated by Bacillus tequilensis KXF6501 fermentation
YANG Kuo, LIU Di, WU Jianrong, DENG Jia, WANG Fang
2024, 41 (6):  62.  doi: 10.3969/j.issn.2095-1736.2024.06.062
Abstract ( 73 )   PDF  
This experiment was implemented to investigate the differences of the metabolites in blueberry fruits after the application ofBacillus tequilensisfermentation. The resistance mechanism of postharvest blueberry fruits treated byB.tequilensisfermentation was explored and the theoretical references for the application ofB.tequilensisin blueberry fruits storage and preservation were provided. Blueberry fruits were treated withB.tequilensisfermentation, the lesion diameters were measured. Subsequently, non-targeted metabolomics techniques were used to analyze the metabolites of blueberry fruits treated withB.tequilensisfermentation. Then, the content of phenolic compounds and flavonoids in blueberry fruit was determined. The result showed that blueberry fruit lesion diameters were significantly reduced after treated withB.tequilensisfermentation. Based on non-targeted metabolomics techniques, 77 pathways were annotated, and a total of 27 significant differential metabolites were enriched in the 20 metabolic pathways with the highest correlation in the group after treated withB.tequilensisfermentation. Among them, six pathways with significant influence were listed as follows: biosynthesis of phenylpropanoids, flavone and flavonol biosynthesis, biosynthesis of siderophore group nonribosomal peptides, flavonoid biosynthesis, apoptosis and C5-Branched dibasic acid metabolism. And the content of phenolic compounds and flavonoids in blueberry fruit was higher than that in the control treatment, which was consistent with the results of metabolomics analysis. After the treated withB.tequilensisfermentation, secondary metabolites accumulated in blueberry fruit, which in turn increased the disease resistance of blueberry fruits.
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Cloning, prokaryotic expression and monclone antibody prepartion of IFN-γ of Acipenser baerii
TIAN Zhaohui
2024, 41 (6):  69.  doi: 10.3969/j.issn.2095-1736.2024.06.069
Abstract ( 94 )   PDF  
Using transcriptome information, the readable coding frame (ORF) 528 bp encoding 176 amino acids ofIFN-γofAcipenser baeriiwas cloned with the characteristic sequence and nuclear localization sequence of IFN-γ. According to this sequence, a prokaryotic expression vector Pet30α-IFN-γwas constructed containing 6His (histidine) at the n-terminal. Then the Pet30α-IFN-γplasmid was transformed intoEscherichia coliBL21(DE3), which was induced by IPTG (isopropyl-β-d-thiogalactoside), and the expressed recombinant protein was identified by SDS-PAGE electrophoresis and Western Blot. The recombinant protein was 22.8 ku, mainly existing in the form of inclusion bodies, and the optimal expression condition was induced at 37 ℃ with 0.75 mmol /L IPTG for 6 h. The purified recombinant protein was obtained by nickel column chromatography. The recombinant protein was immunized to mice, 8 positive cell lines were obtained by cell fusion, monoclonal antibody purified by protein G affinity chromatography, the valence of antibody was 2×105, which laid a foundation for further study of the immunological function of IFN-γ ofAcipenser baerii.
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Analysis on difference of agronomic characters and nutritional quality of mixed sowing for triticale and pea in the Qaidam Basin
MA Wenyan, CAO Dong, LIU Baolong, WANG Dongxia
2024, 41 (6):  74.  doi: 10.3969/j.issn.2095-1736.2024.06.074
Abstract ( 68 )   PDF  
This study focused on effect of gramineae-legume mixed sowing on its agronomic and quality traits for triticale and peas, and selection of the suitable planting mode for the Qaidam Basin. It has important guiding significance for alleviating the supply-demand contradiction of forage in this region. The results showed that, compared to single sown, the mixed sowing extended the growth period of triticale by 2d. Under the mixed sowing ratio of Qingsimai 2+Qingjian 1 (P2S2) was 0.7∶0.3, the tillering number per plant increased by 4.50%, 69.69% and 44.99%, compared with that of the control (P2S1), and the same mixed sowing ratio groups of Qingsimai 1+Qingjian 1(P1S2)and Qingsimai 6+Qingjian 1(P3S2), respectively. The dry grass yield of P2S2 combination increased by 17.61% compared with that of the P2S1. The crude protein, crude fat, calcium, and phosphorus content of the forage showed an increasing trend under different mixed sowing combinations of grass and beans. Using the grey relational comprehensive evaluation method, the mixed sowing of Qingsimai 2 and Qingjian 1 (0.7∶0.3) showed the excellent performance of agronomic and quality traits. This treatment was the best suitable cultivation mode for mixed sowing of triticale and peas, and there was greater potential for promoting and applying in Qaidam Basin.
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New distribution record of the Myricaria laxiflora inMinjiang River of Sichuan Province
GAO Gang, CHEN Xuyan, LI Yihang, TANG Jing
2024, 41 (6):  82.  doi: 10.3969/j.issn.2095-1736.2024.06.082
Abstract ( 50 )   PDF  
Six new distribution populations ofMyricaria laxiflora(Franch.) P. Y. Zhang & Y. J. Zhang were reported , which is a national Ⅱ-class protective plant. These populations are distributed in pebble beach of Minjiang River in Xuzhou District and Cuiping District, Yibin City, Sichuan Province. This is the first time that a naturally distributed population has been found in the Minjiang River, and these populations are also the most upstream population. By this discovery, the distribution area of the species was broadened, that is of great significance for the conservation of the species. The voucher specimen (GAO2022003) was preserved in the herbarium room of Yibin University.
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Solanum nitidibaccatum Bitter. a newly naturalized species of Solanaceae in China
SONG Wendan, MA Zhancang, PAN Chengnan, NAN Zhanyuan, YAN Ping
2024, 41 (6):  84.  doi: 10.3969/j.issn.2095-1736.2024.06.084
Abstract ( 76 )   PDF  
During the plant diversity survey in Xinjiang in 2022-2023, the author found a Solanaceae plant in Hami City, Xinjiang, which had not been reported in the literature or recorded in specimens in China, and was distinctly different from other Solanaceae plants in China. Through literature review and comparative morphological characterization, it was identified asSolanum nitidibaccatumBitter., a newly recorded plant in China. Investigation for two consecutive years found that this species has multiple wild populations in Hami City, Xinjiang and has a tendency to spread. This paper, based on the English description of the morphological characters ofS. nitidibaccatumBitter., describes its morphological characteristics in Chinese in combination with the voucher specimen, and reports its geographic distribution and biological characteristics. It aims to provide new information for the study of species diversity of Solanaceae and the monitoring and control of invasive alien plants. The voucher specimen is preserved in the Herbarium of Shihezi University (SHI).
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Effects of mercury stress on physiological characteristics of forage grass in plateau
ZHAO Mingde, WANG Jin, LI Xiaoxiao
2024, 41 (6):  87.  doi: 10.3969/j.issn.2095-1736.2024.06.087
Abstract ( 82 )   PDF  
In order to reduce the damage of heavy metals to soil, the tolerance and the enrichment effect of heavy metal Hg2+ on Elymus sibiricus and Poa crymophila under different mass concentrations were studied. In this study, pot experiments were conducted at room temperature, different concentrations of Hg2+ (HgCl2, 0, 25, 50, 75 and 100 mg/L) and Hoagland aqueous solution were used to treat plant. The results showed that under different concentrations of heavy metal stress, Poa crymophila showed strong tolerance ability, while Elymus sibiricus showed weak tolerance. In addition, the enrichment ability of heavy metals in Poa crymophila was significantly higher than that in Elymus sibiricus, and the enrichment ability of heavy metals in intercropping Poa crymophila was higher than that in monoculture Poa crymophila. There was no significant difference in enrichment ability between monoculture Poa crymophila and intercropping Elymus sibiricus, but they all showed different degrees of enrichment ability. On the whole, Poa crymophila can be used as a preferred plant for remediation of heavy metal contaminated soil, especially intercropping Poa crymophila has the most significant enrichment effect on heavy metals, and can also improve the soil ecological environment.
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Frontier research of plant stem cells: from basic theory to application
CHEN Liu , ZHANG Jixiang, CHEN Chunli
2024, 41 (6):  92.  doi: 10.3969/j.issn.2095-1736.2024.06.092
Abstract ( 128 )   PDF  
From Nov 17th to Nov 20th, 2023, the first national plant stem cell on biology conference, led by the Chinese society for cell biology, was held in Wuhan, China. The conference focused on cutting-edge research in the theory and application of plant stem cells, from the comparison of commonalities and characteristics of plant and animal stem cells, the regulation of plant stem cell activity, plant regeneration, the interaction between plant stem cells and the environment, and the new progress of single-cell sequencing technology and interdisciplinary research in plant stem cells. And plant stem cells in modern biotechnology including tissue culture, grafting, cutting and other asexual reproduction, plant genome editing and genetic transformation, crop molecular breeding, and synthetic biology of plant stem cell chassis applications and other aspects were also reported and exchanged. The convening of the conference effectively promoted the development of domestic plant stem cell theory and applied research, played an important role in cultivating reserve talents in this field, and built a platform for equal exchange and active cooperation for participants.
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Research progress on the effects of BDNF on central nervous system diseases #br# #br#
SONG Xueqing, SUN Yalun, ZHOU Lifang, WANG Songhua, MENG Wei
2024, 41 (6):  96.  doi: 10.3969/j.issn.2095-1736.2024.06.096
Abstract ( 99 )   PDF  
This article introduced the important role of brain-derived neurotrophic factor (BDNF) in central nervous system (CNS) function and related diseases, including promoting neuronal growth and survival, enhancing synaptic plasticity, participating in the regulation of neurotransmitters, promoting myelination, and neuroprotective effects, which suggested that BDNF plays an important role in the improvement and treatment of CNS diseases. By summarizing the animal experiments and preclinical studies related to BDNF, the research progress on the effects of BDNF on CNS diseases, such as spinal cord injury, traumatic brain injury, ischemic brain injury, Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, epilepsy and depression, and the molecular mechanism of BDNF in CNS diseases were summarized, which provided ideas for exploring the effects of BDNF in the treatment of related CNS diseases. In the future, the pharmacokinetics mechanism of BDNF can be explored in experimental animals, and different delivery methods of BDNF and its derivatives can be developed to treat CNS related diseases.
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Rapid non-destructive identification of heavy metal contaminated clams based on hyperspectral technology#br#
LIU Zhongyan, YANG Junjie, QIAO Muxi, LIU Yao
2024, 41 (6):  104.  doi: 10.3969/j.issn.2095-1736.2024.06.104
Abstract ( 68 )   PDF  
To explore a new method for identifying heavy metal contaminated clams, a hyperspectral spectrometer was used to collect reflectance spectra in the range of 450-900 nm of normal and heavy metal contaminated clams. The Multiple Scatter Correction(MSC) method was employed to eliminate interference factors in the spectra. Six methods, including Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA), Local Linear Embedding (LLE), Independent Component Analysis (ICA), Multidimensional Scaling (MDS), and Isometric Mapping (ISOMAP), were used to reduce the dimensionality of the data. Four classifiers, namely K-Nearest Neighbors (KNN), LogitBoost, Support Vector Machine (SVM), and GradientBoosting, were applied to classify 800 contaminated clams with heavy metals(cadmium (Cd), copper (Cu), lead (Pb) and zinc (Zn)) and normal clams. The results showed that all four classifiers performed well on the spectra reduced by LDA, with the LogitBoost classifier achieving an averageAccuracyof 99.40% and anF-measureof 97.99%, outperforming the other classifers. Furthermore, under imbalanced sample class sizes, classifying each type of heavy metal contaminated and normal clams separately further confirmed the robustness of the MSC-LDA-LogitBoost identification model. This study confirmed the feasibility of using hyperspectral technology combined with machine learning method to identify heavy metal contaminated clams.
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Exploration of “integration of medicine, industry, education and research” in the training model of master’s student in laboratory medicine
ZHOU Qiang, WANG Qin, YE Naifang, ZHANG Hao, LIU Zhou, YAO Jie
2024, 41 (6):  111.  doi: 10.3969/j.issn.2095-1736.2024.06.111
Abstract ( 78 )   PDF  
How to cultivate new generation medical laboratory talents in the new medical background with innovative spirit and practical ability has become one of the major issues urgently needed to be solved in the subject of laboratory medicine. This article analyzes the problems existing in the training process of laboratory medicine master’s students, combines the demand of society for innovative talents in laboratory medicine, and constructs a collaborative education system. Mainly from the perspective of “integration of medicine, industry, teaching and research”, the curriculum system is optimized, with modules such as “basic knowledge, professional knowledge, innovative courses, and humanities literacy” established, discussing the training mode that combines the training of medical laboratory graduate students with laboratory medicine, biomedical technology, and engineering innovation. By adopting diversified teaching modes such as traditional courses, experimental courses, field visits, student lectures, online and offline combination, etc., the students’ ability to think innovatively in biomedical technology and interdisciplinary integration, and constructing a medical-engineering integrated talent training mode guided by practical clinical scientific problems is cultivated to meet the high-quality requirements of the new era.
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Teaching design and practice of DNA barcode technology in plant taxonomy experimental course
ZHOU Yadong, YANG Guo, GUAN Bicai
2024, 41 (6):  116.  doi: 10.3969/j.issn.2095-1736.2024.06.116
Abstract ( 89 )   PDF  
Plant taxonomy experiments have always been a crucial component of university botany courses, serving as a sgnificant practical link in the pursuit of mastering plant taxonomy. In traditional teaching methods, teachers usually establish a fundamental system of plant classification for students depend on campus plants, specimen halls, parks, and nature reserves. With the rapid advancement of biotechnology, however, people have begun to utilize the genetic information carried within plant DNA to identify plant species. The emerging field of plant DNA barcoding technology has gradually become both a thriving research area and an efficient plant identification method. Therefore, incorporating this technology into plant taxonomy experiments in universities can not only enhance students’ comprehension and grasp of plant taxonomy knowledge, but also improve their molecular biology experimental skills and scientific experimental literacy. In this study, common gymnosperms found on campus were taken as a case in point to study their DNA barcodes usingITS2andrbcLsequences, to explore the phylogenetic relationships of these groups, and to provide molecular evidence for their accurate identification as well. Meanwhile, a teaching design was proposed based on plant DNA barcode technology in university plant taxonomy experiments that allows students to comprehend the characteristics and evolutionary relationships of common campus plants from both a morphological and molecular perspective.
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Innovative reform and practice of introduction to life sciences under the goal of diverse talents education
LI Junlin, WEI Ning, ZHANG Yu, FU Aigen, SHI Zhangyan, CUI Jihong, CHEN Fulin, ZHANG Yani, GUAN Feng
2024, 41 (6):  121.  doi: 10.3969/j.issn.2095-1736.2024.06.121
Abstract ( 84 )   PDF  
How to implement the goal of diversified talent cultivation in higher education has become an urgent problem. In the context of the “free change of major” situation and disciplinary system adjustment in comprehensive universities, innovative reform was carried out on the Introduction to Life Sciences, enabling it to play a positive role in implementing the goal. The results showed as follows: the personalized and diversified developmental needs of students were supported by the interdisciplinary and integration resources integrated from biological science, biotechnology, ecology, and traditional Chinese medicine; interdisciplinary and integrated teaching teams could enable freshmen to establish timely connections with professional teachers, and selectively join research teams based on their interests and aspirations; maximizing the role of leading course of Introduction to Life Sciences, freshmen could quickly understand the characteristics of various disciplines and teacher resources. Independent development was promoted by autonomous choice from interdisciplinary and integration resources, and promoted by actively participating in diverse pathways, such as scientific research training, innovation and entrepreneurship ability cultivation, and professional and social practice. Students were guided to transform from cognitive to practical innovation and entrepreneurship; the training goal of diverse talents was effectively and long-term implemented by process and diverse valuations which could be used to adjust the teaching content and mode timely. This reform and practical achievements suggest that the continuous intersection and integration of relevant disciplines can meet the personalized and diversified needs of student development under the premise of institutional and resource guarantees; fully utilizing the function of pilot courses and strengthening the connection of educational processes can effectively promote personalized and diversified independent development; a diversified evaluation system can make the education process more effective and long-lasting.
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Exploration and practice in cell biology teaching by using Arabidopsis thaliana auxin transport mutants #br#
LIU Linchuan, MAO Juan, WANG Yongfei, MA Sanmei
2024, 41 (6):  126.  doi: 10.3969/j.issn.2095-1736.2024.06.126
Abstract ( 87 )   PDF  
In our study, we investigated the use of auxin transport mutants ofArabidopsis thalianaas a model for teaching cell biology. Through discussion-based teaching methods, phenotypic observation and analysis, and subcellular localization experiments, students can gain a deep understanding of the relationship between membrane protein structure and their functions, emphasizing the significance of transmembrane transport in plant growth and development. In addition to fostering students’ interest, this course promotes a comprehensive understanding and application of knowledge in cell biology and related fields, thereby enhancing critical thinking and scientific research skills. Taken together, our teaching approach cultivates innovative individuals capable of thriving in interdisciplinary collaborations.
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