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Research status and development trends of bioleaching technology YANG Baojun, LIU Yang, LIU Hongchang, LI Yongmei, GAN Min, WANG Jun, LIAO Rui, ZHANG Ke, ZHU Zhenyu, QIU Guanzhou Journal of Biology    2024, 41 (3): 1-.   DOI: 10.3969/j.issn.2095-1736.2024.03.001 Abstract （480）      PDF       Knowledge map    The development and the utilization of low-grade mineral resources constrain global green and sustainable development. Bioleaching technology is a cutting-edge technology for achieving green and sustainable development in mineral processing and non-ferrous metal metallurgy and plays an important role inin-situremediation of heavy metal pollution sites in mines. This article reviewed the basic concepts, microbial physiological characteristics, and mechanisms of action of biotechnology, as well as the application and research status of biotechnology in copper ore extraction, refractory gold ore oxidation pretreatment, uranium ore extraction, and rare earth extraction. In the future, research should be conducted on the selection of high-efficiency leaching microorganisms, the interface mechanism of bioleaching, high-efficiency energy-saving devices, and engineering technologies, and further expanding the application fields of bioleaching technology. Related Articles | Metrics

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The antichlamydial activity of berberine in vitro XIE Wenxia, ZHU Yixin, BAO Xiaofeng Journal of Biology    2024, 41 (5): 1-.   DOI: 10.3969/j.issn.2095-1736.2024.05.001 Abstract （324）      PDF       Knowledge map    The aim of this study was to elucidate the antichlamydial activity and mechanism of the natural product berberinein vitro. Firstly, the antichlamydial activity of berberine was evaluated. The data showed that berberine inhibited the formation of inclusion body and the titer of infectious progenies in a dose-dependent manner for bothChlamydia trachomatisL2 andChlamydia muridarumMoPn. Secondly, host cell viability was measured with CCK-8 kit to evaluate the cytotoxicity of the compound. No significant host cytotoxicity was observed when berberine concentration was not higher than 8 μmol/L. Then, the effect of berberine on the chlamydial infection process was examined by the pretreatment of compound with host cells orChlamydia. Pretreatment assays showed that berberine could weakly directly act onChlamydiabut not host cells to disturb the chlamydial infection process. Finally, the effect of berberine on the chlamydial proliferation process was analyzed. Later treatment and withdrawal assay revealed that the EB→RB transformation stage at 2－12 h of chlamydial period is the mainly target for berberine. The long-term tolerance assay displayed that berberine delayed the chlamydial development cycle, but could not kill it completely. In conclusion, berberine has a broad-spectrum antichlamydial activityin vitro, and its inhibitory effect on the proliferation period was stronger than that on the infection process. Related Articles | Metrics

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Comparative study on interactions between Myxococcus xanthus/Pseudomonas aeruginosa and ionic rare earth minerals #br# WANG Mengyuan, HUANG Shiyun, LIU Hongchang, LIU Yang, LI Jingna, NIE Zhenyuan, XIA Jinlan, WANG Jun Journal of Biology    2024, 41 (3): 11-.   DOI: 10.3969/j.issn.2095-1736.2024.03.011 Abstract （251）      PDF       Knowledge map    In this paper,Myxococcus xanthusandPseudomonas aeruginosa, which are common in the rare earth mining environment, were prepared, and the solution behaviors of the two bacteria and their fermentation broth during the interaction with ion-type rare earth ore were comparatively studied. The morphology, structure, distribution of rare earth elements and functional groups of the cells and mineral phases were analyzed by means of scanning electron microscopy, transmission electron microscopy, X-ray diffraction and Fourier infrared spectroscopy. The results showed that there were some differences in the interaction processes of theM. xanthusandP. aeruginosaon rare earth minerals. In the leaching process of rare earth minerals, the direct-action system ofP. aeruginosawas better than that of the indirect-action system of the fermentation broth, while the direct-action system of theM. xanthuswas weaker than that of the indirect action system of the fermentation liquid. In addition, the presence of rare earth minerals can promote the secretion of lipid and protein components ofM. xanthusand inhibit the secretion of polysaccharide components, while forP. aeruginosa, the presence of rare earth minerals has little effect on the secretion of lipid and protein components but promotes the secretion of polysaccharide components. Related Articles | Metrics

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Frontier research of plant stem cells: from basic theory to application CHEN Liu , ZHANG Jixiang, CHEN Chunli Journal of Biology    2024, 41 (6): 92-.   DOI: 10.3969/j.issn.2095-1736.2024.06.092 Abstract （246）      PDF       Knowledge map    From Nov 17th to Nov 20th, 2023, the first national plant stem cell on biology conference, led by the Chinese society for cell biology, was held in Wuhan, China. The conference focused on cutting-edge research in the theory and application of plant stem cells, from the comparison of commonalities and characteristics of plant and animal stem cells, the regulation of plant stem cell activity, plant regeneration, the interaction between plant stem cells and the environment, and the new progress of single-cell sequencing technology and interdisciplinary research in plant stem cells. And plant stem cells in modern biotechnology including tissue culture, grafting, cutting and other asexual reproduction, plant genome editing and genetic transformation, crop molecular breeding, and synthetic biology of plant stem cell chassis applications and other aspects were also reported and exchanged. The convening of the conference effectively promoted the development of domestic plant stem cell theory and applied research, played an important role in cultivating reserve talents in this field, and built a platform for equal exchange and active cooperation for participants. Related Articles | Metrics

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Research progress on the effects of BDNF on central nervous system diseases #br# #br# SONG Xueqing, SUN Yalun, ZHOU Lifang, WANG Songhua, MENG Wei Journal of Biology    2024, 41 (6): 96-.   DOI: 10.3969/j.issn.2095-1736.2024.06.096 Abstract （246）      PDF       Knowledge map    This article introduced the important role of brain-derived neurotrophic factor (BDNF) in central nervous system (CNS) function and related diseases, including promoting neuronal growth and survival, enhancing synaptic plasticity, participating in the regulation of neurotransmitters, promoting myelination, and neuroprotective effects, which suggested that BDNF plays an important role in the improvement and treatment of CNS diseases. By summarizing the animal experiments and preclinical studies related to BDNF, the research progress on the effects of BDNF on CNS diseases, such as spinal cord injury, traumatic brain injury, ischemic brain injury, Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, epilepsy and depression, and the molecular mechanism of BDNF in CNS diseases were summarized, which provided ideas for exploring the effects of BDNF in the treatment of related CNS diseases. In the future, the pharmacokinetics mechanism of BDNF can be explored in experimental animals, and different delivery methods of BDNF and its derivatives can be developed to treat CNS related diseases. Related Articles | Metrics

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Space microbiology and its bioengineering applications SUN Lichao, ZHAO Pengzhuo, HU Wei, HUO Yixin Journal of Biology    2024, 41 (6): 1-.   DOI: 10.3969/j.issn.2095-1736.2024.06.001 Abstract （218）      PDF       Knowledge map    Investigating the behavior and response mechanisms of microorganisms in space environments or simulated space environments aids in understanding their adaptability and evolutionary principles in extreme conditions. In this study, the impacts of microorganisms on the space environment were analyzed, and the techniques and equipments for studying space microbiology were introduced. Molecular mechanisms underlying microbial growth and morphology, mass transfer, secondary metabolite production, immune characteristics, biofilm formation, DNA damage and stress responses under space microgravity and radiation were thoroughly reviewed. The potential of space microbiology in aerospace breeding and biomedical engineering were further prospected. This work holds significant implications for space breeding, provides scientific guidance for space missions, and advances the development of manned spaceflight and space exploration. Related Articles | Metrics

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Application and prospect of neutralizing antibodies in the treatment of viral infectious diseases HU Jing, DENG Shasha, WANG Meihua, LIU Mengyao, JIN Tengchuan Journal of Biology    2025, 42 (1): 1-.   DOI: 10.3969/j.issn.2095-1736.2025.01.001 Abstract （204）      PDF       Knowledge map    Infectious diseases are a kind of transmitted diseases caused by pathogenic microorganisms, which can affect human and animal health and even produce great social economic burden. Compared with vaccination, antiviral drugs and other control methods, antibody has gradually become an important strategy for the treatment of infectious diseases because of its small side effects and strong timeliness. Neutralizing antibody is a kind of antibody that can specifically bind to the surface antigen of infectious particles, thereby blocking its interaction with the host cells and preventing cells from being invaded. A variety of neutralizing antibodies against viral infection have been developed. In this paper, the research progress of four typical virus therapeutic antibodies for SARS-CoV-2, Ebola, HIV and RSV was reviewed, including representative antibodies targeting the SARS-CoV-2 S protein, ebola GP protein, HIV gp160 protein and RSV F protein. The selection of neutralizing antibody target and its future development and application were discussed. Related Articles | Metrics

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Research progress on bioleaching of yttrium from rare earths #br# YAN Linlin, WANG Hailan, XIAO Chunqiao, FANG Yun Journal of Biology    2024, 41 (3): 61-.   DOI: 10.3969/j.issn.2095-1736.2024.03.061 Abstract （200）      PDF       Knowledge map    The occurrence and distribution of rare earth yttrium (Y) in nature, the main chemical extraction and biological extraction methods were reviewed, and the relationship between rare earth yttrium and microorganisms was discussed from the aspects of rare earth yttrium bioleaching (microbial adsorption, microbial metabolite leaching) and the effect of rare earth yttrium on microorganisms. The biological extraction of rare earth yttrium has broad prospects, but it still faces many problems, such as the lack of dominant leaching strains and the unclear mechanism of mineral-microbial interaction. On this basis, this paper further put forward some suggestions, such as establishing a high-throughput microbial screening method and rapid identification of functional strains, strengthening the interaction mechanism between microorganisms and yttrium at the molecular level and the selective leaching of yttrium by microorganisms, popularizing the biological leaching of microbial metabolites, and optimizing the in-situ extraction technology, hoping to provide reference for the design and development of yttrium resources in China. Related Articles | Metrics

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Identification and analysis of differential expression genes regulated by UHRF1 in breast cancer cells #br# GONG Chunxue, DONG Qincai, LIU Xuan, CAO Cheng Journal of Biology    2024, 41 (6): 12-.   DOI: 10.3969/j.issn.2095-1736.2024.06.012 Abstract （198）      PDF       Knowledge map    Ubiquitin-like with PHD and Ring finger domains 1 (UHRF1) is an epigenetic regulator that maintains DNA methylation modification and plays critical roles in embryonic development, tumor progress and prognosis. In this study, the differential expression genes regulated by UHRF1 was identified, and the associated cell functions, metabolic pathways, and related diseases these genes involved were investigated. Total RNA extracted from UHRF1 knockdown cells (MCF-7/shRNA-UHRF1) or scramble cells (MCF-7/shRNA-Scramble) as a control was subjected to RNA-Seq analysis, then the differential expression genes were screened by using DESeq2 software and partially validated by qRT-PCR. Gene ontology (GO), Kyoto Encylopedia of Genes and Genomes (KEGG) and Disease ontology (DO) analyses were employed to investigate the potential biological functions of these genes. The results showed that a total of 2926 genes expression were regulated by UHRF1. Among them, 1453 genes were down-regulated and 1473 genes were up-regulated by UHRF1 knockdown. GO and KEGG analysis showed that these genes were primarily enriched in cell metabolism, immune regulation, signal transduction, cardiovascular disease and tumorigenesis. DO analysis showed that they were mainly involved in cancer and neurological diseases. These results suggested that DNA methylation mediated by the epigenetic regulatory factor UHRF1 can regulate the differential expression of genes at the transcriptional level, providing data support for the participation of UHRF1 in the regulation of relevant signaling pathways. Related Articles | Metrics

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AsmiR156: potential hub in drought memory molecular regulatory network of Agriophyllum squarrosum (L.) Moq. #br# FANG Tingzhou, AWUKU Ibrahim, YAN Xia, QIAN Chaoju, FAN Xingke, ZHAO Pengshu, LIAO Yuqiu, SHI Liang, MA Xiaofei Journal of Biology    2024, 41 (4): 1-.   DOI: 10.3969/j.issn.2095-1736.2024.04.001 Abstract （196）      PDF       Knowledge map    In order to detect how miRNA-mRNA interaction participates in drought response and drought memory ofA. squarrosum, the drought-sensitive ecotype AEX ofAgriophyllum squarrosumwas selected as the plant material, and transcriptome and sRNA sequencing were performed after three rounds of drought-rehydration treatments. A total of 341 and 7858A. squarrosummiRNAs (DEMIs) and mRNAs (DEMs) were identified in response to drought stress, respectively. The DEMI-DEM regulatory network showed that 24A. squarrosumDEMIs were involved in the regulation of 1423 DEMs, and these target genes were mainly involved in cell differentiation function. Two drought memory miRNAs (DMMIs), miR156 and miR6173, were identified, which regulated 11 and 6 drought memory genes (DMGs), respectively. Target genes for miR156 included three transcription factors (TFs):AsRWP-RK,AsC3H11, andAsWRKY33; the target genes for miR6173 included twoGSTs (glutathione S-transferase). In previous study,AsC3H11andAsWRKY33participated in theA. squarrosumdrought memory hierarchical regulatory network as TF molecular switches, soA. squarrosummiR156 could serve as a candidate hub for the “miRNA-transcription factor-functional gene” network. This study partially elucidated the molecular regulatory basis of drought response and drought memory inA. squarrosum, which will provide a theoretical basis for the development of stress-tolerant genetic resources in desert plants. Related Articles | Metrics

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Research progress on quorum sensing mediating bacterial-bacteriophage interactions GAO Miao, LING Baodong Journal of Biology    2024, 41 (5): 94-.   DOI: 10.3969/j.issn.2095-1736.2024.05.094 Abstract （195）      PDF       Knowledge map    The quorum sensing system, as a critically important communication mechanism among microorganisms, has a close and intricate interaction with the bacteria-bacteriophage. This paper reviewed the role of quorum sensing systems in mediating the formation of bacterial biofilms, the synthesis of various surface proteins in bacteria, the role of prokaryotic adaptive immune systems in resisting bacteriophage infections, and regulation of the switch of phage lysis-lysogeny to optimize phage survival and reproduction. By summarizing the research results on the interactions between the quorum sensing system and bacteria- bacteriophage, it was hoped this effort would provide a comprehensive exploration of the mechanisms underlying the quorum sensing system interaction between bacteria-bacteriophages，which provided the theoretical basis for phage control of the spread and treatment of drug-resistant pathogens. Related Articles | Metrics

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Isolation, identification and nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification bacterium ZH7 YANG Zongzheng, MENG Zhu, JIAO Yongjie, YAN Huijuan, ZUO Zihan, ZHANG Jing, WU Zhiguo Journal of Biology    2024, 41 (5): 34-.   DOI: 10.3969/j.issn.2095-1736.2024.05.034 Abstract （192）      PDF       Knowledge map    A heterotrophic nitrification-aerobic denitrification strain ZH7 was isolated from aquaculture pond sediment. The strain ZH7 was identified asAcinetobactersp. by morphological observations, physiological and biochemical assays and 16S rDNA sequencing. The impact of various factors (carbon source, C/N ratio, temperature, pH, shaking speed, NaCl concentration) on the strain’s growth and denitrification performance was investigated. And the heterotrophic nitrification and aerobic denitrification performance of the strain in single nitrogen source and mixed nitrogen source under the optimal conditions was also investigated. The results showed that the strain ZH7 could use sodium acetate as a carbon source, and achieved removal rates of ammonium nitrogen (NH+4-N) and nitrate nitrogen (NO－3-N) exceeding 95% under conditions of C/N of 12－16, temperature of 30 ℃, shaking speed of 180 r/min and pH value of 7－9. In mixed nitrogen source conditions (ammonium and nitrate nitrogen), the strain preferentially utilized ammonium nitrogen. The nitrogen removal process followed the Compertz model, with the maximum removal rate for ammonia nitrogen significantly higher than that for nitrate nitrogen and nitrite nitrogen, withRmof 12.61, 7.17 and 7.23 mg/(L·h), respectively. The strain ZH7 demonstrated the efficient denitrification capabilities and can be considered a potential candidate for biological denitrification. Related Articles | Metrics

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Inhibition of non-enzymatic glycosylation by exopolysaccharides of Bifidobacterium longum XIA Junying, YU Haixia, QI Shimei, ZHANG Funa, WU Jing, XIAO Weihua Journal of Biology    2024, 41 (5): 6-.   DOI: 10.3969/j.issn.2095-1736.2024.05.006 Abstract （190）      PDF       Knowledge map    The extracellular polysaccharide extract (ZJ1-EPS) ofBifidobacterium longumZJ1 strain isolated from the feces of centenarians was used to explore the effect and mechanism of ZJ1-EPS inhibition of AGEs by pyruvaldehyde (MGO)-induced bovine serum albumin (BSA) and non-enzymatic glycosylation (AGEs) in human skin cells and glucose-induced zebrafish AGEs, respectively. The results showed that ZJ1-EPS had a significant inhibitory effect on the formation of products in the middle and late stages of protein non-enzymatic glycosylation. This study revealed that the exopolysaccharide extract ofBifidobacteriumhas the effect of inhibiting the production of AGEs, suggesting its mechanism of action and application potential for skin anti-aging. Related Articles | Metrics

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Preliminary analysis of CtDXR gene copy number,salt tolerance and heat tolerance in Transgenic Nicotiana benthamiana#br# TIAN Chunyao, JI Huiyue, DING Runyue, ZHENG Qiaomu, ZHOU Jiayu, LIAO Hai Journal of Biology    2024, 41 (4): 17-.   DOI: 10.3969/j.issn.2095-1736.2024.04.017 Abstract （189）      PDF       Knowledge map    To evaluate the copy number ofCtDXRgene in transgenicNicotiana benthamianaand further analyze its tolerance to salt and heat stress, Southern Blot was applied to determine the copy number of theActingene in wild-typeN. benthamianaand the copy number of theCtDXRgene inCtDXRtransgenicN. benthamianawas detected by real-time PCR withActingene as reference gene. Then, the salt and heat tolerance ofCtDXRtransgenicN. benthamianawas analyzed. According to the PCR-dependent method, 10 of T1 transgenicN. benthamianawere randomly detected and all of which obtained positive results, indicating thatCtDXRhas been successfully transformed. TheActingene is a single-copy gene in the genome of wild-typeN. benthamiana. 80% of transgenicN.benthamianawere determined as single-copy or low-copy lines.CtDXRtransgenicN. benthamianashowed better performance, such as plant height (P<0.01), number of lateral roots (P<0.01), and fresh weight (P<0.001), than wild-typeN. benthamianaunder salt stress, whereasCtDXRtransgenicN. benthamianaexhibited better performance, such as plant height (P<0.01), number of leaves (P<0.01), and fresh weight, than wild-typeN. benthamianaunder heat stress. Taken together,CtDXRconferred salt and heat tolerance toN.benthamiana. In this study, a method to detect copy number of foreign gene in transgenicN. benthamianawas established, by which the copy number ofCtDXRgene in transgenicN. benthamianawas accomplished. Moreover,CtDXRgene was preliminarily identified to have salt and heat tolerance. Related Articles | Metrics

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Effect of the deletion and mutation of Gly33 on the antioxidant activity of hTrx-1 GUO Xijun, YANG Jikang, HUANG Junwei, WANG Tong, LIU Xinqiao, QI Xingzhu Journal of Biology    2024, 41 (4): 11-.   DOI: 10.3969/j.issn.2095-1736.2024.04.011 Abstract （185）      PDF       Knowledge map    In order to investigate whether Gly33 residue in the conserved sequence of the active site of human thioredoxin hTrx-1 is essential in the process of reducing oxidative protein, the Gly33 residue of hTrx-1 was deleted or replaced (G→A) by two synthetic mutant genes. The synthetic mutant genes were then expressed in prokaryotic cells, further purified and tested for antioxidant activity. The results showed that both the deletion of Gly33 residue and the mutation to Ala, the mutated hTrx-1 could be efficiently expressed in prokaryotic cells, indicating that both mutant hTrx-1 could form a “Trx fold” structure. Antioxidant activity testing showed that in the absence of Gly33 residues, its antioxidant capacity decreased, when Gly33 mutated to Ala, its antioxidant capacity significantly decreased to near loss. Therefore, this article speculated that the change of conserved amino acid residues in the Trx-1 active center affects the geometric configuration of the active center region, resulting in difficulty in approaching the substrate protein in space. The detection results of the content of disulfide bond sulfhydryl groups involved in the formation of disulfide bonds showed that the content of disulfide bond sulfhydryl groups formed in mutant hTrx-1 was significantly lower than that in wild-type hTrx-1. It was speculated that the ability of Cys32 and Cys35 to form disulfide bonds in mutant hTrx-1 was affected. Related Articles | Metrics

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砷黄铁矿生物氧化影响因素及控制方法 HONG Maoxin, LIU Yuling, YANG Baojun, WANG Jun Journal of Biology    2024, 41 (3): 52-.   DOI: 10.3969/j.issn.2095-1736.2024.03.052 Abstract （180）      PDF       Knowledge map    Bio-oxidation of arsenopyrite is a major contributor to the generation of arsenic-containing acid mine drainage. This paper summarized the research status of key influencing factors and source control technologies related to arsenopyrite bio-oxidation. The physical and chemical properties, as well as the chemical and biological oxidation processes, of arsenopyrite were introduced. Detailed explanations were provided regarding the impact and mechanisms of environmental factors, such as microorganisms, co-existing ions, organic matters, and associated minerals, on arsenopyrite bio-oxidation. The oxidation of arsenopyrite and the release of arsenic into the environment are predominantly driven by water, oxygen, and microorganisms. The process can be inhibited by isolating any of these components, thereby preventing the formation of acid mine drainage. Consequently, the advantages and disadvantages of present source control methods such as oxygen barriers, bactericides, co-disposal, and passivation were analyzed and compared. Besides, potential research directions in this field were also outlined, providing a theoretical foundation for evaluating and mitigating arsenic-containing acid mine drainage caused by arsenopyrite bio-oxidation. Related Articles | Metrics

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Study on the environmental DNA preservation conditions of fishes LIU Yang, YING Fang, YANG Jun, WANG Huanying, HONG Wenjie, WANG Tingzhang Journal of Biology    2024, 41 (3): 114-.   DOI: 10.3969/j.issn.2095-1736.2024.03.114 Abstract （179）      PDF       Knowledge map    The optimal preservation method and preservation time for DNA from the simulated water samples of reared fish was studied. Water samples at the same volume were pretreated with 0.45 μm filter membranes inanucleic acid-free environment. Comparison of the obtained quality of eDNA, PCR concentrations of fish 12S rRNA gene, and the amplicon sequencing results based on the available amplicon sequence variant method by combination of different preservation method and preservation time were studied. The results showed that with six preservation methods (－20 ℃ freezing, －80 ℃ freezing, ethyl alcohol at room temperature, 75% ethanol at room temperature, Longmire buffer at room temperature, and TK buffer at room temperature), the TK buffer at room temperature was the best eDNA captured methods, followed by freezing (－20 ℃, －80 ℃). And the －20 ℃, －80 ℃, and TK buffer at room temperature had good stability under the same preservation time respectively, and could stably preserve the filter membrane within two weeks to maximize the genetic information of fish in water samples. This study provided technical reference for third-party gene detection laboratories for fish eDNA preservation strategies and facilitating the development of eDNA metabarcoding technology. Related Articles | Metrics

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Exploration and practice of synthetic biology curricular system construction to promote the cultivation of top-notch innovative talents WANG Dongmei, HONG Jiong Journal of Biology    2024, 41 (5): 122-.   DOI: 10.3969/j.issn.2095-1736.2024.05.122 Abstract （178）      PDF       Knowledge map    The talent cultivation is the main core competitiveness of the “Double First-Class” construction in universities, and the construction of a high-quality curriculum system is an important support for improving the quality of talent cultivation. With the goal of cultivating top-notch innovative talents, an integrated innovative talent cultivation model of “synthetic biology course-the second classroom-competition” has been formed through more than ten years of teaching exploration and practice in our university. This curricular system consists of a high-quality teaching team from USTC and the Chinese Academy of Sciences, course content of the hot topics and lately development of synthetic biology, online and offline mixed teaching mode including virtual simulate experiment and micro lessons, practical skills training and cross-specialty cooperation in “encoding life” association, and the research training of high-level biology competitions represented by the international genetically engineered machine (iGEM) competition. All these together dramatically improved undergraduate students’ ability of self-learning, scientific research innovation, and team collaboration. Up to now, excellent results have been achieved in the international and domestic competitions such as iGEM, the International Directional Evolution Competition, and the Synthetic Biology Competition, continuously advancing towards the goal of cultivating top-notch innovative students with ideals, responsibilities, and excellence in character and learning. Related Articles | Metrics

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Effects of Lycium barbarum exosomes on proliferation inhibition and apoptosis of non-small cell lung cancer A549 cells ZHANG Liyun, WANG Zehua, MA Chunyan Journal of Biology    2024, 41 (5): 48-.   DOI: 10.3969/j.issn.2095-1736.2024.05.048 Abstract （177）      PDF       Knowledge map    In order to clarify the effect ofLycium barbarumexosomes on proliferation inhibition and apoptosis of non-small cell lung cancer cell A549, the exosomes were extracted by ultra-fast centrifugation method. The morphology of exosomes was detected by transmission electron microscopy, and the diameter distribution of exosomes was detected by particle size analysis. A549 cells were treated with the extractedL. barbarumexosomes. The effect ofL. barbarumexosomes on A549 cells proliferation was detected by CCK-8 method, EdU was used to detect the effect ofL. barbarumexosomes on A549 cells, the migration efficiency of cells was detected by cell scratch method and Transwell, the cloning ability of cells was detected by cell cloning method.The apoptosis rate of cells was detected by flow cytometry, Western Blot and qRT-PCR were used to detect the protein and gene expression of apoptosis pathway. The results showed that the extracted exosomes were saucer-like under electron microscope, and the size was 40－200 nm, which was consistent with the shape characteristics of the exosomes. Compared with control group,L. barbarumexosomes group could inhibit A549 cell proliferation in a concentration-dependent way (P<0.01). The cell migration ability and cell cloning ability in different concentrations ofL. barbarumberry exosomes decreased in a concentration-dependent manner (P<0.01), while the cell apoptosis rate increased in a concentration-dependent manner (P<0.01).L. barbarumexosomes could up-regulate the pro-apoptotic factors such as Cl-Caspase3, p53 and Bax in a concentration dependent manner, and down-regulate the proteins and genes of anti-apoptotic factors such as Bcl-2 (P<0.01). The conclusion was thatL. barbarumexosomes could inhibit the proliferation of A549 cells and promote the apoptosis of A549 cells, andL. barbarumexosomes could also induce apoptosis through mitochondrial pathway. Related Articles | Metrics

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Study and analysis of root exudates of Lavandula angustifolia at different cultivation years SHI Renzeng, DENG Xia, WANG Junzhu, LIU Wenwen, LI Guihua, GUO Zixuan, JIAO Ziwei Journal of Biology    2024, 41 (5): 61-.   DOI: 10.3969/j.issn.2095-1736.2024.05.061 Abstract （166）      PDF       Knowledge map    To explore the impact of different cultivation periods on the exudates ofLavandula angustifoliain Yili, Xinjiang, non-targeted metabolomic analysis was conducted by using liquid chromatography-mass spectrometry (LC-MS) on the rhizosphere soil samples of 1-year-old, 3-year-old, and 5-year-old lavender plants(named L1, L3, and L5), as well as soil samples without lavender cultivation (L0). The results indicated that root exudates included nine main categories: organic acids, lipids, nucleic acids, carbohydrates, vitamins and cofactors, hormones, peptides, antibiotics, and steroids. Compared to L0, L1, L3, and L5 resulted in the identification of 26, 17, and 21 significantly different exudates, respectively (P＜0.001). Additionally, L1, L3, and L5 exhibited enrichment in 13, 10, and 9 significantly different metabolic pathways (P＜0.05), respectively. Compared to L1, L3 and L5 showed 27 (P＜0.05) and 9 significantly different exudates (P＜0.001), respectively, and enrichment in 4 and 9 significantly different metabolic pathways (P＜0.05), respectively. Furthermore, when compared to L3, L5 presented 13 significantly different exudates (P＜0.05) and enrichment in 6 significantly different metabolic pathways (P＜0.05). Overall, the exudates ofLavandula angustifoliaroot system underwent significant changes as the cultivation period extended, with sterols initially decreasing and then increasing, while vitamins, peptides, and nucleotides gradually decreased. Related Articles | Metrics