Top Read Articles

Published in last 1 year |

In last 2 years |

In last 3 years |

All

Please wait a minute...


For Selected: Download Citations EndNote Reference Manager ProCite BibTeX RefWorks Toggle Thumbnails

Select

Research progress on carbon reduction potential of agriculture in China ZHOU Xinyan, ZHENG Huabao Journal of Biology    2024, 41 (2): 1-.   DOI: 10.3969/j.issn.2095-1736.2024.02.001 Abstract （309）      PDF       Knowledge map    This review provided an overview of the main characteristics and types of agricultural carbon emissions in China. It also identified potential areas for carbon reduction in agriculture, named farmland utilization, livestock and poultry breeding, agricultural waste disposal, and land use management. Reasonable agronomic management and economic policy measures can effectively reduce carbon emissions from agricultural land use. Measures such as reducing agricultural carbon emissions at the source, implementing process control, and employing end-of-pipe treatment techniques are effective in mitigating carbon emissions from livestock and poultry farming. Additionally, optimizing manure management and agricultural straw utilization can minimize carbon emissions from the disposal of agricultural waste. Furthermore, adopting an optimized structure for land resource utilization, implementing intensive land use practices, and constructing high-standard farmland are crucial measures for carbon reduction in land use management. This article serves as a valuable reference for enhancing the potential of carbon reduction in China’s agriculture. Related Articles | Metrics

Select

Research status and development trends of bioleaching technology YANG Baojun, LIU Yang, LIU Hongchang, LI Yongmei, GAN Min, WANG Jun, LIAO Rui, ZHANG Ke, ZHU Zhenyu, QIU Guanzhou Journal of Biology    2024, 41 (3): 1-.   DOI: 10.3969/j.issn.2095-1736.2024.03.001 Abstract （279）      PDF       Knowledge map    The development and the utilization of low-grade mineral resources constrain global green and sustainable development. Bioleaching technology is a cutting-edge technology for achieving green and sustainable development in mineral processing and non-ferrous metal metallurgy and plays an important role inin-situremediation of heavy metal pollution sites in mines. This article reviewed the basic concepts, microbial physiological characteristics, and mechanisms of action of biotechnology, as well as the application and research status of biotechnology in copper ore extraction, refractory gold ore oxidation pretreatment, uranium ore extraction, and rare earth extraction. In the future, research should be conducted on the selection of high-efficiency leaching microorganisms, the interface mechanism of bioleaching, high-efficiency energy-saving devices, and engineering technologies, and further expanding the application fields of bioleaching technology. Related Articles | Metrics

Select

Marine biotechnology for the “blue seed industry” QIN Song, SUN Lin, WANG Yajun, LIU Zhengyi, WANG Yinchu, ZHANG Jian Journal of Biology    2024, 41 (1): 1-.   DOI: 10.3969/j.issn.2095-1736.2024.01.001 Abstract （227）      PDF       Knowledge map    The article reviews the theoretical and practical innovations of marine biotechnology in China over the past 30 years, from the perspective of the cutting across development of China’s “blue seed industry”. It also analyses the international development trends and the urgent needs for the sustainable and healthy development of China’s mariculture industry, and concludes that regionally responsive precision breeding for global changes is the direction of marine biotechnology for “blue agriculture” in the coming decade. Related Articles | Metrics

Select

The analysis of somatic and dendritic morphology of cerebellar Purkinje cells in mice at different ages PEI Pei, TANG Zhengquan Journal of Biology    2024, 41 (1): 26-.   DOI: 10.3969/j.issn.2095-1736 Abstract （214）      PDF       Knowledge map    In order to investigate the difference in morphology of cerebellar Purkinje cells in mice at different ages, mice were divided into four age groups of five animals each corresponding to 14-day, 1-month, 2-month, and 1-year old mice. Parvalbumin selected as a unique marker for Purkinje cells, using immunochemistry, we observed and compared somatic and dendritic morphology of cerebellar Purkinje cells of these mice in four groups under a confocal microscope. The results showed that the dendritic length of cerebellar Purkinje cells in 2-month old mice was significantly higher than that in other groups (P<0.05), and the dendritic length of cerebellar Purkinje cells in 1-year old mice was significantly lower than that in other groups (P<0.05), as well as the somatic sizes of Purkinje cells of mice in 4 age groups showed a decreasing trend with age. In this study, we also analyzed the fluorescence intensity of parvalbumin in cerebellar Purkinje cells of mice in 4 age groups, which showed an overall increasing trend with age. Together, the results indicate that cell body size and dendritic length of cerebellar Purkinje are associated with age, with an opposite trend of parvalbumin expression and its development. This study suggests that parvalbumin may play an important role in regulation of cerebellar Purkinje cell development. Related Articles | Metrics

Select

Engineering of a carboxylic acid reductase-in the synthesis of pharmaceutical intermediate (S)-2-aminobutanol ZHANG Xiaohui, QIN Zongmin, LI Congcong, LU Fuping, QU Ge, SUN Zhoutong Journal of Biology    2024, 41 (1): 6-.   DOI: 10.3969/j.issn.2095-1736.2024.01.006 Abstract （213）      PDF       Knowledge map    Armed with both hydroxyl and amino groups, (S)-2-aminobutanol is an essential chiral building block used in the preparation of diverse pharmaceutical drugs, while effective biocatalysts are highly desired toward the synthesis of (S)-2-aminobutanol. The carboxylic acid reductase derived from Segniliparus rugosus (SrCAR) was used as the model enzyme in the transformation of (S)-2-aminobutyric acid to (S)-2-aminobutanol combined with a heterologous alcohol dehydrogenase. After screening the previously constructed SrCAR mutant libraries, the active site was obtained and co-evolution analysis was performed. By using the combinatorial active-site saturation test strategy, the dominant mutant XH7 (G430V/E533F/A627N) was obtained. The kcat/Kmand Tmvalues were improved 2.1 fold and 2.3 ℃ compared to that of the wild type enzyme, respectively in the transformation of N-Boc-(S)-2-aminobutyric acid to N-Boc-(S)-2-aminobutyraldehyde. Further, N-Boc-(S)-2-aminobutyraldehyde to the alcohol product can be reduced by the introduction of Pseudomonas fluorescens (PfADH). The bi-enzymatic system containing of XH7 and PfADH enabled a 99% conversion toward 20 mmol/L substrate in 5 h. After deprotection and purification, the yield of (S)-2-aminobutanol reached 60%. Molecular dynamics simulations were performed to shed light on the molecular basis for the improved activity and thermostability of the best mutant. This study expands the biocatalytic toolbox for the synthesis of (S)-2-aminobutanol, and provides guidance for the biosynthesis of similar high value-added pharmaceutical intermediates. Related Articles | Metrics

Select

Comparative study on interactions between Myxococcus xanthus/Pseudomonas aeruginosa and ionic rare earth minerals #br# WANG Mengyuan, HUANG Shiyun, LIU Hongchang, LIU Yang, LI Jingna, NIE Zhenyuan, XIA Jinlan, WANG Jun Journal of Biology    2024, 41 (3): 11-.   DOI: 10.3969/j.issn.2095-1736.2024.03.011 Abstract （197）      PDF       Knowledge map    In this paper,Myxococcus xanthusandPseudomonas aeruginosa, which are common in the rare earth mining environment, were prepared, and the solution behaviors of the two bacteria and their fermentation broth during the interaction with ion-type rare earth ore were comparatively studied. The morphology, structure, distribution of rare earth elements and functional groups of the cells and mineral phases were analyzed by means of scanning electron microscopy, transmission electron microscopy, X-ray diffraction and Fourier infrared spectroscopy. The results showed that there were some differences in the interaction processes of theM. xanthusandP. aeruginosaon rare earth minerals. In the leaching process of rare earth minerals, the direct-action system ofP. aeruginosawas better than that of the indirect-action system of the fermentation broth, while the direct-action system of theM. xanthuswas weaker than that of the indirect action system of the fermentation liquid. In addition, the presence of rare earth minerals can promote the secretion of lipid and protein components ofM. xanthusand inhibit the secretion of polysaccharide components, while forP. aeruginosa, the presence of rare earth minerals has little effect on the secretion of lipid and protein components but promotes the secretion of polysaccharide components. Related Articles | Metrics

Select

creening and heterologous expression of ω-transaminase for the preparation of S-methoxyisopropylamine ZHANG Tao, ZHOU Haisheng, XU Jiaqi, YANG Lirong Journal of Biology    2024, 41 (2): 8-.   DOI: 10.3969/j.issn.2095-1736.2024.02.008 Abstract （181）      PDF       Knowledge map    An enzyme library containing 36 ω-transaminase was constructed according to the evolutionary classification of the aminotransferase family and substrate specificity. Genes from the library were cloned into E.coli BL21 (DE3) for heterologous recombinant expression of the enzymes. The enzyme expression levels were analyzed and corresponding enzyme activity and enantiomeric selectivity were determined. Through screening, the best ω-aminotransferase was found to be the one from Bacillus megaterium (BmeTA), which exhibited a crude enzyme activity of 2.0 U/mL and a pure enzyme activity of 9.5 U/mg protein. Enzymology characterization showed that the optimal temperature of BmeTA was 35 ℃, and the optimal pH was 8.0. Based on these, the catalytic reaction process conditions were further optimized, and 450 mmol/L of S-methoxyisopropylamine was obtained after an 18 h catalytic reaction under the conditions of 20 g/L crude enzyme and 0.5 mmol/L coenzyme dosage, and 1.4 amino donor/amino acceptor ratio, reaching a 90% conversion rate of substrate. The investigation of this study laid the foundation for the industrialization of biocatalytic preparation of S-methoxyisopropylamine. Related Articles | Metrics

Select

The antichlamydial activity of berberine in vitro XIE Wenxia, ZHU Yixin, BAO Xiaofeng Journal of Biology    2024, 41 (5): 1-.   DOI: 10.3969/j.issn.2095-1736.2024.05.001 Abstract （166）      PDF       Knowledge map    The aim of this study was to elucidate the antichlamydial activity and mechanism of the natural product berberinein vitro. Firstly, the antichlamydial activity of berberine was evaluated. The data showed that berberine inhibited the formation of inclusion body and the titer of infectious progenies in a dose-dependent manner for bothChlamydia trachomatisL2 andChlamydia muridarumMoPn. Secondly, host cell viability was measured with CCK-8 kit to evaluate the cytotoxicity of the compound. No significant host cytotoxicity was observed when berberine concentration was not higher than 8 μmol/L. Then, the effect of berberine on the chlamydial infection process was examined by the pretreatment of compound with host cells orChlamydia. Pretreatment assays showed that berberine could weakly directly act onChlamydiabut not host cells to disturb the chlamydial infection process. Finally, the effect of berberine on the chlamydial proliferation process was analyzed. Later treatment and withdrawal assay revealed that the EB→RB transformation stage at 2－12 h of chlamydial period is the mainly target for berberine. The long-term tolerance assay displayed that berberine delayed the chlamydial development cycle, but could not kill it completely. In conclusion, berberine has a broad-spectrum antichlamydial activityin vitro, and its inhibitory effect on the proliferation period was stronger than that on the infection process. Related Articles | Metrics

Select

Heterologous expression, purification and enzymatic properties of dopa decarboxylase from Pseudomonas ZHOU Yaolin, SUN Dengyue, ZENG Zhixiong, LI Xia Journal of Biology    2024, 41 (1): 14-.   DOI: 10.3969/j.issn.2095-1736.2024.01.014 Abstract （164）      PDF       Knowledge map    The dopamine decarboxylase (DODC) gene sequence from Pseudomonas was PCR amplified, double digested and ligated to the vector CV6-pGEX-6P-1, and the expression vector CV6-pGEX-6P-1-DODC was successfully constructed by validation and sequencing. Escherichia coli BL21 (DE3) was transferred for recombinant expression. The OD value was 0.6-0.8, the final concentration of isopropyl β-D-1-thiogalactoside (IPTG) was 0.1 mmol/L, and cultured at 16 ℃ overnight for 12-16 h. The results showed that DODC fusion protein with high expression was obtained in E.coli BL21(DE3) by induction expression. DODC purified protein with purity above 95% was obtained by GST-affinity chromatography, 3C protease digestion and ion exchange chromatography. The enzymatic properties of DODC were studied. The optimum reaction temperature of the enzyme was 40 ℃, which was sensitive to the effect of temperature, and the enzyme activity was more than 80% at 20-30 ℃. The enzyme activity decreased substantially above 30 ℃. The optimal buffer solution was PBS buffer solution, the optimal reaction pH was 7.5, the optimal substrate was L-DOPA. The metal cation Ca2+ promoted the enzyme activity. The sequence homology analysis showed that DODC from Pseudomonas belongs to the AAT-Ⅰ superfamily, and the conserved catalytic active site of the enzyme was predicted to be Thr 241. Related Articles | Metrics

Select

Mechanism and research advances of hydrogen production by green algae #br# Journal of Biology    2024, 41 (1): 100-.   DOI: 10.3969/j.issn.2095-1736.2023.06.100 Abstract （164）      PDF       Knowledge map    This review systematically introduced three different pathways of hydrogen production by hydrogenase in green algae under anaerobic condition, including direct hydrogen production via photosystem II, indirect hydrogen production independent of photosystem II and hydrogen production by dark fermentation. The advantages and disadvantages of each pathway were analyzed. However, limited by technology and high costs, hydrogen production by green algae was still in the laboratory-scale, and will take a long time to achieve industrial production. Aiming at the efficient hydrogen production, this review described the latest outcome in this field and summarized the methods for optimizing the hydrogen production, including hydrogenase modification, reducing the oxygen content, lowering the cyclic electron transfer rate, reducing the Calvin-cycle, remodeling the light harvesting complex, pretreatment of the biomass and photo-bioreactor design. The future development of hydrogen production by green algae was also discussed. Related Articles | Metrics

Select

Structure and function of chitinase gene in crustaceans ZHANG Di, JIN Xin, CHENG Runqiang, GENG Changsheng, LI Ye Journal of Biology    2024, 41 (2): 91-.   DOI: 10.3969/j.issn.2095-1736.2024.02.091 Abstract （156）      PDF       Knowledge map    The classification of chitinases in 50 crustaceans was reviewed and it was verified that chitinases in crustaceans could be subdivided into group 1-group 7 according to the classification of amino acid homology. The structure and the function of chitinase in crustaceans were focused in this review. In terms of structure, the characterization of conserved motifs based on amino acid sequence, as well as the composition profile of several domains of group 1-group 7 chitinases were discussed. Then, the three-dimensional structure of one of chitinases from P. trituberculatus, which was considered as a symbol of the crustacean chitinase, was predicted. The functions of crustacean chitinase were further stated and it generally participated in the digestion of chitinous food, in the digestion of old shells during molting, as well as degrading the chitinous cell wall of pathogen to exert its function on immune defense. Related Articles | Metrics

Select

AsmiR156: potential hub in drought memory molecular regulatory network of Agriophyllum squarrosum (L.) Moq. #br# FANG Tingzhou, AWUKU Ibrahim, YAN Xia, QIAN Chaoju, FAN Xingke, ZHAO Pengshu, LIAO Yuqiu, SHI Liang, MA Xiaofei Journal of Biology    2024, 41 (4): 1-.   DOI: 10.3969/j.issn.2095-1736.2024.04.001 Abstract （152）      PDF       Knowledge map    In order to detect how miRNA-mRNA interaction participates in drought response and drought memory ofA. squarrosum, the drought-sensitive ecotype AEX ofAgriophyllum squarrosumwas selected as the plant material, and transcriptome and sRNA sequencing were performed after three rounds of drought-rehydration treatments. A total of 341 and 7858A. squarrosummiRNAs (DEMIs) and mRNAs (DEMs) were identified in response to drought stress, respectively. The DEMI-DEM regulatory network showed that 24A. squarrosumDEMIs were involved in the regulation of 1423 DEMs, and these target genes were mainly involved in cell differentiation function. Two drought memory miRNAs (DMMIs), miR156 and miR6173, were identified, which regulated 11 and 6 drought memory genes (DMGs), respectively. Target genes for miR156 included three transcription factors (TFs):AsRWP-RK,AsC3H11, andAsWRKY33; the target genes for miR6173 included twoGSTs (glutathione S-transferase). In previous study,AsC3H11andAsWRKY33participated in theA. squarrosumdrought memory hierarchical regulatory network as TF molecular switches, soA. squarrosummiR156 could serve as a candidate hub for the “miRNA-transcription factor-functional gene” network. This study partially elucidated the molecular regulatory basis of drought response and drought memory inA. squarrosum, which will provide a theoretical basis for the development of stress-tolerant genetic resources in desert plants. Related Articles | Metrics

Select

Identification of key enzyme genes involved in lupeol synthesis pathway in Syneilesis aconitifolia by transcriptome analysis ZHANG Jingjing, XU Jingyao, SHAN Tingyu, ZHAO Liqiang, ZHONG Xinxin, ZHANG Shuaishuai, WU Jiawen Journal of Biology    2024, 41 (1): 32-.   DOI: 10.3969/j.issn.2095-1736.2024.01.032 Abstract （147）      PDF       Knowledge map    order to analyze the lupeol biosynthetic pathway in Syneilesis aconitifolia and explore its key enzyme genes, DNBSEQ sequencing platform was used to sequence the transcriptome of leaves, stems, roots and rhizomes of Syneilesis aconitifolia, and 191 541 Unigenes were obtained after de novo assembly. KEGG metabolic pathway analysis showed that 961 Unigenes were involved in the lupeol biosynthetic pathway in Syneilesis aconitifolia, of which 395 Unigenes encoded 17 key enzymes of the biosynthetic pathway. Comparing root with other tissues, 24 shared differentially expressed genes were involved in the lupeol biosynthetic pathway, encoding farnesyl diphosphate synthase (FPPS), squalene synthase (SS), squalene epoxidase (SE) and other key enzymes. Structural analysis of the key enzymes FPPS, SS and SE, showed that they all had conserved catalytic domains and substrate binding domains. This work enriched the functional gene database of Syneilesis aconitifolia, and laid a foundation for further study of the lupeol biosynthetic pathway and the function and regulation mechanism of key enzyme genes. Related Articles | Metrics

Select

Review of the functions of exosomes in aquatic organisms CAI Chengjian, JIANG Jiachao, LUO Ping, WANG Lizhang, ZHANG Yuanyuan, GU Xiujun Journal of Biology    2024, 41 (2): 97-.   DOI: 10.3969/j.issn.2095-1736.2024.02.097 Abstract （145）      PDF       Knowledge map    Exosomes are small, membrane-bound vesicles with a diameter ranging from 30 to 150 nm. They are actively released by diverse cell types and exhibit sustained secretion under both physiological and pathological conditions of tissue cells. Exosomes can be found in various body fluids and exert their influence on recipient cells through the delivery of proteins, lipids, nucleic acids, and other bioactive molecules. These molecular cargos enable exosomes to modulate and alter the behavior of target cells, thereby playing significant roles in intercellular communication and immune regulation, among other crucial cellular processes. This article took aquatic-derived exosomes as a focal point, providing a comprehensive synthesis of their compositional characteristics and elucidating the functional diversity of exosomes that observed across different aquatic organisms. Moreover, it undertook an in-depth analysis of the environmental factors impacting the secretion of exosomes, aiming to unravel the intricate relationship between exosome release quantity and these environmental cues. This review presented novel research insights for the study of aquatic-derived exosomes in the context of environmental conservation. Related Articles | Metrics

Select

Nobel Prize cases-based teaching practice in “Molecules and cells”,a fundamental course of the speciality of biomedical engineering JIANG Hui, LI Yan Journal of Biology    2024, 41 (1): 120-.   DOI: 10.3969/j.issn.2095-1736.2023.06.120 Abstract （144）      PDF       Knowledge map    In recent years, we have explored the pilot reform of Nobel Prize cases as a main clue to guide the course of “Molecules and Cells”. We selected 15 award cases covering 11 core chapters of the course, each of which was jointly investigated by two students. The students are recommended to use the ceremony report of Nobel Prize winner as a blueprint, focusing on the research background, the highlights of discoveries/inventions, and the theoretical or applied values by the scientists. In the in-class practice, a standard unit of introduction-report-discussion-summary is constructed, using the method of panel discussion to improve teaching effectiveness. The strategy integrates our national needs, science and art, facing challenges, and innovative spirit. This plan has stimulated students’ enthusiasm for self-directed learning, cultivated students’ basic biomedical qualities and concepts through extracurricular research and in-class discussions. This reform has achieved beneficial performances under limited course time and 90% students have achieved significant results after course learning. Through teaching practice, we have further proposed improvement ideas such as method optimization, viewpoint update, ideological and political integration, and perspective enhancement, which has potential reference significance for the teaching reform of life foundation courses in biomedical majors. Related Articles | Metrics

Select

Reassembly between two distinct G-quadruplexes BI Xinmeng, FU Wenqiang, ZHANG Na, WANG Tao Journal of Biology    2024, 41 (2): 25-.   DOI: 10.3969/j.issn.2095-1736.2024.02.025 Abstract （144）      PDF       Knowledge map    Here, mainly through solution NMR experiments, we found that the mutant sequence of Thrombin Binding Aptamer (TBA-M) and human telomeric sequence htel3 self-fold into G-quadruplexes in sodium solution, respectively. Appealingly, these two well pre-folded G-quadruplexes can further reassemble into a new heteromolecular G-quadruplex complex (TBA-M/htel3) spontaneously through DNA strand displacement. The stoichiometric ratio between TBA-M and htel3 in the complex is identified as 1∶1. The last three guanosines (G14G15G16) from the 3′-end of TBA-M are involved in the formation of the G-quadruplex core of TBA-M/htel3 complex via Hoogsteen base pairing. In this paper, we reveal for the first time that two well pre-folded G-quadruplexes enable a further Hoogsteen pairing-based DNA strand displacement, in which the reaction process and molecular mechanism are also investigated primarily. Our finding expands the understanding of the interacting pattern and recognition mechanism between differently folded structures of nucleic acids. Related Articles | Metrics

Select

AITC regulates serotonin secretion and other biological functions in enterochromaffin cells WANG Siyu, YANG Yali, SI Qiqi, GUO Tailin, HUANG Xinhe Journal of Biology    2024, 41 (2): 51-.   DOI: 10.3969/j.issn.2095-1736.2024.02.051 Abstract （140）      PDF       Knowledge map    In order to investigate the effects of allyl isothiocyanate (AITC) on the synthesis of 5-HT in EC and the biological functions of EC, edible grade AITC were used to intervene with RIN-14B cells (rat pancreatic endocrine cell line), a cell model of EC. The calcium ion concentration in the cells was detected by FLUO-8 AM, the expression level of genes related to 5-HT synthesis was analyzed by qRT-PCR , the levels of 5-HT were determined by UPLC, and the transcriptome of AITC-treated RIN-14B cells was detected and analyzed by RNA-seq and bioinformatics.The results indicated that AITC caused an increase in intracellular calcium ion concentration through activation of TRPA1, while upregulated the expression ofTph1(tryptophan hydroxylase) and Ddc (5-hydroxytryptophan decarboxylase). In addition, GO and KEGG functional enrichment analysis on AITC treated RIN-14B cells showed that AITC mainly regulated glutathione metabolism and relate pathways such as antioxidant and inflammatory regulation, suggesting that AITC may regulate intestinal homeostasis by stimulating EC to promote glutathione metabolism, and also participate in inflammatory regulation of the intestine. The above results provided experimental data and research ideas for further study of the effects of AITC on EC and deeper biological functions of the intestine. Related Articles | Metrics

Select

Antiviral components of Salvia tiliifolia against tobacco mosaic virus WANG Zifu, YAN Junyi, LIU Yang, WU Jiangmei, YAN Xiaohui, HU Shijun Journal of Biology    2024, 41 (1): 68-.   DOI: 10.3969/j.issn.2095-1736.2024.01.068 Abstract （140）      PDF       Knowledge map    In order to find new plant virus inhibitors, tobacco mosaic virus (TMV) was used as the test virus. It was found that the extract of Salvia tiliifolia, an invasive plant, showed anti-TMV activity. Screening by the half-leaf method along with different treatment pattern of extract, the petroleum ether extract showed the strongest protective effect and inactivation effect at the concentration of 1 mg/mL, with inhibition rate of 40.95% and 52.85% respectively. The ethyl acetate extract showed the best curative effect with the inhibition rate of 52.85%, which was not significantly different from Ningnanmycin, a positive control. Among fractions of petroleum ether extracts, Fr7 showed the best protective effect and curative effect, with the inhibition rate of 40.51% and 45.52% respectively, and Fr6 showed potent inactive effect with the inhibition rate of 74.63%. Among fractions of ethyl acetate extracts, Fr1′ showed the strongest protective effect with an inhibition rate of 61.77%, Fr3′ displayed the best curative effect with an inhibition rate of 55.02%, and Fr4′ showed potent inactive effect with the inhibition rate of 60.22%. Five compounds were separated and identified by modern chromatographic spectrum technologies, they were tilifodiolide, eupatoric acid, ursolic acid, oleanolic acid, and 2-methyl-5,7-dioxy chromone. Related Articles | Metrics

Select

Reform and innovation of the course genetic engineering under the background of new agricultural science FANG Zhongming Journal of Biology    2024, 41 (1): 126-.   DOI: 10.3969/j.issn.2095-1736.2024.01.126 Abstract （137）      PDF       Knowledge map    The construction of the “new agricultural science” requires the reform of the links and methods of cultivating agricultural and forestry talents, and the cultivation of innovative, composite, and practical new agricultural talents. Based on this, our university has carried out course reform and innovation from three aspects: editing and publishing the textbook “genetic engineering”, and creating a teaching system of genetic engineering with production application as the main line; and reforming the course syllabus, lesson plans and courseware using the BOPPPS method for teaching design; organizing and participating in disciplinary competitions to promote teaching and learning through competition. The organic connection of the three teaching links before class, during class, and after class was achieved, the rationality of the teaching system, the feasibility of teaching design, and the effectiveness of teaching guidance were tested, the comprehensive qualities and innovative abilities of students were effectively cultivated, and the construction of the national first-class undergraduate major “agricultural science” was promoted through the “three step method” of innovation, reform, and competition mentioned above. It could provide reference for the cultivation of innovative and high-quality agricultural talents in the new era. Related Articles | Metrics

Select

Anti-respiratory syncytial virus activity evaluation via primary human airway epithelial cell culture DING Huiru, ZHAO Min, CHENG Ningning, FU Yuanhui, PENG Xianglei, YU Jiemei, ZHENG Yanpeng, HE Jinsheng Journal of Biology    2024, 41 (1): 20-.   DOI: 10.3969/j.issn.2095-1736.2024.01.020 Abstract （136）      PDF       Knowledge map    This study aims to establish the culture method of human primary airway epithelial cell (hAEC) and to investigate the anti-respiratory syncytial virus (RSV) activity and mechanism of 3-thioindole compound RSVA-4 and immunosuppressive metabolite 6-MMPR using hAEC system, which intends to construct a cell model for RSV drug screening and efficacy evaluation. The respiratory tract epithelial cells from volunteers were collected and cultured, then the morphology, activity and purity were identified. The anti-RSV activity and cytotoxicity of RSVA-4 and 6-MMPR were further verified in hAEC system. The mechanism of RSVA-4 and 6-MMPR accounting for the suppression of RSV replication on hAEC was explored by using fluorescence real-time quantitative PCR (RT-qPCR) and time-of-addition assay. The survival rate of cultured hAEC was 93.51% as determined by trypan blue staining. The half maximal inhibitory concentrations (IC50) of RSV-A-4 and 6-MMPR were （207.30±4.77） μmol/L and （3191.00±6.11） μmol/L, respectively. The half maximal cytotoxic concentration (CC50) of 6-MMPR was （95526.00±10.97） μmol/L, while no toxicity of RSVA-4 was observed on hAEC. Mechanistically, RSV-A-4 and 6-MMPr inhibited RSV replication in the genome replication/transcription phase. The hAEC culture method was successfully established, which could be used to screen and evaluate the anti-RSV drugs in vitro. RSVA-4 and 6-MMPR could effectively inhibit RSV replication at the cellular level. Altogether, the result could provide an experimental basis for the research and development of RSV drug and pathogenesis. Related Articles | Metrics