Abstract: The extracellular polysaccharide extract (ZJ1-EPS) ofBifidobacterium longumZJ1 strain isolated from the feces of centenarians was used to explore the effect and mechanism of ZJ1-EPS inhibition of AGEs by pyruvaldehyde (MGO)-induced bovine serum albumin (BSA) and non-enzymatic glycosylation (AGEs) in human skin cells and glucose-induced zebrafish AGEs, respectively. The results showed that ZJ1-EPS had a significant inhibitory effect on the formation of products in the middle and late stages of protein non-enzymatic glycosylation. This study revealed that the exopolysaccharide extract ofBifidobacteriumhas the effect of inhibiting the production of AGEs, suggesting its mechanism of action and application potential for skin anti-aging.

Key words: Bifidobacterium longum, extracellular polysaccharides, non-enzymatic glycosylation, skin cells, zebrafish