生物学杂志

Journal of Biology ›› 2019, Vol. 36 ›› Issue (5): 7-.doi: 10.3969/j.issn.2095-1736.2019.05.007

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Construction of kctd1 homozygous mutants in zebrafish via CRISPR/Cas9 system

  

  1. 1. International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306; 2. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306； 3. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
  • Online:2019-10-18 Published:2019-10-11

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Abstract: KCTD1(potassium channel tetramerization domain containing 1) is one of the members of the potassium channel tetramerization domain containing gene family. The proteins in the gene family possess conserved domains including BTB/POZ domain and K-ketra at the N-terminal, and a variable C-terminal. Mutations and improper expression of human KCTD genes are associated with a variety of diseases. We knocked out the kctd1 gene, the orthologue of human KCTD1, mutation of which caused SEN syndrome in zebrafish(Danio rerio) via CRISPR/Cas9. Two types of homozygous mutants +7 bp and-8 bp were obtained after heterozygous F1 screening and F1 in-cross. This research is of great significance for studying the function of KCTD1 gene and the pathogenesis of KCTD1.

Key words: zebrafish, KCTD1, mutants, CRISPR/Cas9

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