Abstract: This article proposed a teaching method that integrated generative artificial intelligence (AI) into project-based teaching, aiming to improve the learning efficiency and effectiveness of biology majors in Python courses. The results showed that generative AI could effectively assist students with different programming foundations to deeply understand theoretical knowledge and broaden their problem-solving thinking in practical project practice, thereby motivating them to collaborate on more challenging projects. This teaching model helped students better apply the Python language to practice and laid a solid foundation for future scientific research and career development.

Key words: Python course, biology majors, project-based learning, generative AI, learning efficiency