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砷黄铁矿生物氧化影响因素及控制方法 HONG Maoxin, LIU Yuling, YANG Baojun, WANG Jun Journal of Biology    2024, 41 (3): 52-.   DOI: 10.3969/j.issn.2095-1736.2024.03.052 Abstract （180）      PDF       Knowledge map    Bio-oxidation of arsenopyrite is a major contributor to the generation of arsenic-containing acid mine drainage. This paper summarized the research status of key influencing factors and source control technologies related to arsenopyrite bio-oxidation. The physical and chemical properties, as well as the chemical and biological oxidation processes, of arsenopyrite were introduced. Detailed explanations were provided regarding the impact and mechanisms of environmental factors, such as microorganisms, co-existing ions, organic matters, and associated minerals, on arsenopyrite bio-oxidation. The oxidation of arsenopyrite and the release of arsenic into the environment are predominantly driven by water, oxygen, and microorganisms. The process can be inhibited by isolating any of these components, thereby preventing the formation of acid mine drainage. Consequently, the advantages and disadvantages of present source control methods such as oxygen barriers, bactericides, co-disposal, and passivation were analyzed and compared. Besides, potential research directions in this field were also outlined, providing a theoretical foundation for evaluating and mitigating arsenic-containing acid mine drainage caused by arsenopyrite bio-oxidation. Related Articles | Metrics

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Utilization of proximity labeling-mass spectrometry technique to identify Ebola virus-associated host factor in Human ZHANG Xun, BAI Yu, LIU Hainan, LIU Xuan, CAO Cheng Journal of Biology    2024, 41 (3): 92-.   DOI: 10.3969/j.issn.2095-1736.2024.03.092 Abstract （143）      PDF       Knowledge map    The fusion protein of VP35 and biotin ligase TurboID was generated, and the Ebola virus minigenome system (EBOV MG) was utilized to simulate the Ebola virus lifecycle and formation of virus inclusion bodies (VIB) in cells. The introduction of exogenous biotin enabled the labeling of proteins to interact with VP35. Among all proximity labeling (PL) labeled proteins, 537 potential VP35-associated host proteins were identified by differential abundance analysis after quantitative mass spectrometry detection. Gene Ontology (GO) analysis found that many enriched proteins were involved in RNA binding related functions. Subsequently, the association of EBOV VP35 with RNA binding proteins EIF4B and ZNF598 was confirmed. Interruption of EIF4B and ZNF598 expression significantly inhibited EBOV trVLP replication. The study highlighted the effectiveness of PL-quantitative mass spectrometry in identifying virus-host interaction proteins, providing a valuable tool for investigating viral pathogenesis and identifying potential antiviral targets. Related Articles | Metrics

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Study on arsenic tolerance of moderately thermophilic bacteria Leptospirillum ferrooxidans and Acidithiobacillus caldus CHEN Yanzhen, ZHANG Guangji, GUO Jiale, YANG Chao Journal of Biology    2024, 41 (3): 34-.   DOI: 10.3969/j.issn.2095-1736.2024.03.034 Abstract （155）      PDF       Knowledge map    In this study, two moderately thermophilic bacteria, includingL. ferrooxidans(Leptospirillum ferrooxidans) andA. caldus(Acidithiobacillus caldus), used in bioleaching processes of refractory gold ores were taken as the main research objects. The effects of valence of arsenic (As(Ⅲ) and As(Ⅴ)) on the bio-oxidation of two moderately thermophilic bacteria were investigated through studied by shake flask experiment. The results were compared with two mesophilic bacteria,A. ferrooxidans(Acidithiobacillus ferrooxidans) andT. thiooxidans(Thiobacillus thiooxidans). The results showed that the tolerance to As(Ⅲ) of moderately thermophilic bacteria was significantly weaker than that of mesophilic bacteria, but both of thermophilic and mesophilic bacteria showed similar tolerance to As(Ⅴ). At the same time, the effect of As(Ⅲ) on iron-oxidizing bacteria in growth was greater than that on sulfur-oxidizing bacteria. It was found that increasing the bacteria inoculation amount and domesticating bacteria was conducive to the adaptation of moderately thermophilic bacteria to arsenic toxicity. In the actual ore pulp system, increasing the inoculation amount could make moderately thermophilic bacteria finally to adapt to the high arsenic environment and obtain better ore bioleaching effect. The highest mass concentration can reach about 1.54 g/L, and the arsenic leaching rate can be up to 70% in only 10 days. Related Articles | Metrics

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Research progress on the effects of BDNF on central nervous system diseases #br# #br# SONG Xueqing, SUN Yalun, ZHOU Lifang, WANG Songhua, MENG Wei Journal of Biology    2024, 41 (6): 96-.   DOI: 10.3969/j.issn.2095-1736.2024.06.096 Abstract （246）      PDF       Knowledge map    This article introduced the important role of brain-derived neurotrophic factor (BDNF) in central nervous system (CNS) function and related diseases, including promoting neuronal growth and survival, enhancing synaptic plasticity, participating in the regulation of neurotransmitters, promoting myelination, and neuroprotective effects, which suggested that BDNF plays an important role in the improvement and treatment of CNS diseases. By summarizing the animal experiments and preclinical studies related to BDNF, the research progress on the effects of BDNF on CNS diseases, such as spinal cord injury, traumatic brain injury, ischemic brain injury, Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, epilepsy and depression, and the molecular mechanism of BDNF in CNS diseases were summarized, which provided ideas for exploring the effects of BDNF in the treatment of related CNS diseases. In the future, the pharmacokinetics mechanism of BDNF can be explored in experimental animals, and different delivery methods of BDNF and its derivatives can be developed to treat CNS related diseases. Related Articles | Metrics

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Study on the environmental DNA preservation conditions of fishes LIU Yang, YING Fang, YANG Jun, WANG Huanying, HONG Wenjie, WANG Tingzhang Journal of Biology    2024, 41 (3): 114-.   DOI: 10.3969/j.issn.2095-1736.2024.03.114 Abstract （179）      PDF       Knowledge map    The optimal preservation method and preservation time for DNA from the simulated water samples of reared fish was studied. Water samples at the same volume were pretreated with 0.45 μm filter membranes inanucleic acid-free environment. Comparison of the obtained quality of eDNA, PCR concentrations of fish 12S rRNA gene, and the amplicon sequencing results based on the available amplicon sequence variant method by combination of different preservation method and preservation time were studied. The results showed that with six preservation methods (－20 ℃ freezing, －80 ℃ freezing, ethyl alcohol at room temperature, 75% ethanol at room temperature, Longmire buffer at room temperature, and TK buffer at room temperature), the TK buffer at room temperature was the best eDNA captured methods, followed by freezing (－20 ℃, －80 ℃). And the －20 ℃, －80 ℃, and TK buffer at room temperature had good stability under the same preservation time respectively, and could stably preserve the filter membrane within two weeks to maximize the genetic information of fish in water samples. This study provided technical reference for third-party gene detection laboratories for fish eDNA preservation strategies and facilitating the development of eDNA metabarcoding technology. Related Articles | Metrics

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#br# Research progress on the application of secondary metabolites of saline Cyanobacteria GUO Min, TAO Yujie, WANG Rong, HAN Rui, ZHU Derui Journal of Biology    2024, 41 (4): 102-.   DOI: 10.3969/j.issn.2095-1736.2024.04.102 Abstract （141）      PDF       Knowledge map    Cyanobacteria are mostly distributed in oceans, salt lakes and hypersaline environments. Due to natural selection and genetic differentiation in salt environments, Cyanobacteria have formed special survival mechanisms including in unique community structure composition, diversity distribution and intracellular secondary metabolites. Currently, the diversity, the dominant genus, the salt adaptation mechanism, the species distribution and application of Cyanobacteria need to be further explored from different salt environments. This article systematically reviewed the recent advances in the population diversity, the community structure compositions and the adaptive survival mechanisms(e.g. accumulation of compatible solutes or antioxidants, balance of intracellular ions, and/or increase of antioxidant enzyme activity) of Cyanobacteria among various salt environments. Further, the representative species of intracellular secondary metabolites (e.g. polyketones, oligopeptides/polypeptides, alkaloids, lipids, terpenes and polysaccharides), chemical structure types and main bioactive functions (e.g. antibacterial, anti-inflammatory, antitumor, antimalarial and protease inhibition) were also systematically summarized. It probably provided a reference for subsequent salina algae mining and the utilization of natural metabolites. Related Articles | Metrics

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Research status and development trends of bioleaching technology YANG Baojun, LIU Yang, LIU Hongchang, LI Yongmei, GAN Min, WANG Jun, LIAO Rui, ZHANG Ke, ZHU Zhenyu, QIU Guanzhou Journal of Biology    2024, 41 (3): 1-.   DOI: 10.3969/j.issn.2095-1736.2024.03.001 Abstract （480）      PDF       Knowledge map    The development and the utilization of low-grade mineral resources constrain global green and sustainable development. Bioleaching technology is a cutting-edge technology for achieving green and sustainable development in mineral processing and non-ferrous metal metallurgy and plays an important role inin-situremediation of heavy metal pollution sites in mines. This article reviewed the basic concepts, microbial physiological characteristics, and mechanisms of action of biotechnology, as well as the application and research status of biotechnology in copper ore extraction, refractory gold ore oxidation pretreatment, uranium ore extraction, and rare earth extraction. In the future, research should be conducted on the selection of high-efficiency leaching microorganisms, the interface mechanism of bioleaching, high-efficiency energy-saving devices, and engineering technologies, and further expanding the application fields of bioleaching technology. Related Articles | Metrics

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Research progress of florigen FT in the function of plant development WANG Yixin, ZHANG Zhongliang, XIE Linan, ZHANG Qingzhu Journal of Biology    2025, 42 (1): 98-.   DOI: 10.3969/j.issn.2095-1736.2025.01.098 Abstract （134）      PDF       Knowledge map    Plants require reproductive growth and vegetative growth to ensure the success of life cycle. It is vital that this developmental process is precisely regulated. Flowering mechanism is highly conserved in plants, the crucial part of it is the transcriptional regulation by signals from both external and internal of the geneFLOWERING LOCUS T (FT), which is known as florigen. FT protein belongs to a highly conserved protein family. Meanwhile, it is also an important factor that both vegetative growth and reproductive growth need. This article introduced the research onFT’s function and mechanism in the two stages, as well as the role of related transcription factors and their regulation network, in an effort to better understand how plants adapt to environmental changes with precision. Additionally, it provided insights for molecular genetic improvements related to plant reproductive regulation in breeding goals. Related Articles | Metrics

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Preliminary analysis of CtDXR gene copy number,salt tolerance and heat tolerance in Transgenic Nicotiana benthamiana#br# TIAN Chunyao, JI Huiyue, DING Runyue, ZHENG Qiaomu, ZHOU Jiayu, LIAO Hai Journal of Biology    2024, 41 (4): 17-.   DOI: 10.3969/j.issn.2095-1736.2024.04.017 Abstract （189）      PDF       Knowledge map    To evaluate the copy number ofCtDXRgene in transgenicNicotiana benthamianaand further analyze its tolerance to salt and heat stress, Southern Blot was applied to determine the copy number of theActingene in wild-typeN. benthamianaand the copy number of theCtDXRgene inCtDXRtransgenicN. benthamianawas detected by real-time PCR withActingene as reference gene. Then, the salt and heat tolerance ofCtDXRtransgenicN. benthamianawas analyzed. According to the PCR-dependent method, 10 of T1 transgenicN. benthamianawere randomly detected and all of which obtained positive results, indicating thatCtDXRhas been successfully transformed. TheActingene is a single-copy gene in the genome of wild-typeN. benthamiana. 80% of transgenicN.benthamianawere determined as single-copy or low-copy lines.CtDXRtransgenicN. benthamianashowed better performance, such as plant height (P<0.01), number of lateral roots (P<0.01), and fresh weight (P<0.001), than wild-typeN. benthamianaunder salt stress, whereasCtDXRtransgenicN. benthamianaexhibited better performance, such as plant height (P<0.01), number of leaves (P<0.01), and fresh weight, than wild-typeN. benthamianaunder heat stress. Taken together,CtDXRconferred salt and heat tolerance toN.benthamiana. In this study, a method to detect copy number of foreign gene in transgenicN. benthamianawas established, by which the copy number ofCtDXRgene in transgenicN. benthamianawas accomplished. Moreover,CtDXRgene was preliminarily identified to have salt and heat tolerance. Related Articles | Metrics

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Inhibition of non-enzymatic glycosylation by exopolysaccharides of Bifidobacterium longum XIA Junying, YU Haixia, QI Shimei, ZHANG Funa, WU Jing, XIAO Weihua Journal of Biology    2024, 41 (5): 6-.   DOI: 10.3969/j.issn.2095-1736.2024.05.006 Abstract （190）      PDF       Knowledge map    The extracellular polysaccharide extract (ZJ1-EPS) ofBifidobacterium longumZJ1 strain isolated from the feces of centenarians was used to explore the effect and mechanism of ZJ1-EPS inhibition of AGEs by pyruvaldehyde (MGO)-induced bovine serum albumin (BSA) and non-enzymatic glycosylation (AGEs) in human skin cells and glucose-induced zebrafish AGEs, respectively. The results showed that ZJ1-EPS had a significant inhibitory effect on the formation of products in the middle and late stages of protein non-enzymatic glycosylation. This study revealed that the exopolysaccharide extract ofBifidobacteriumhas the effect of inhibiting the production of AGEs, suggesting its mechanism of action and application potential for skin anti-aging. Related Articles | Metrics

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Practice of PBL for environmental stress on uneven-colored of tomato fruit ZHANG Xuelian, MENG Xue, LI Nan, WANG Yingxiang Journal of Biology    2024, 41 (5): 115-.   DOI: 10.3969/j.issn.2095-1736.2024.05.115 Abstract （141）      PDF       Knowledge map    The quality problem of uneven-colored of tomato fruit is taken as the breakthrough point for a teaching experiment by using the problem-based learning method. Students were guided to propose the possible environmental factors which affected fruit colour change. And then, they were encouraged to put forward a reasonable hypothesis to explain the reason of uneven-colored fruit. According to the assumption, the teacher helped students to design a comprehensive experiment, including different temperatures treatment combined with different light intensities treatment. The students were guided to confirm that high temperature inhibited the level of lycopene synthesis gene to block the lycopene accumulation, which resulting in an uneven fruit coloring, through the results of fruit phenotype observation, chlorophyll/lycopene content, fruit firmness and gene expression. Through this experiment, students could really feel the close relationship between agricultural production and scientific research, and realize the importance of theory guiding practice. Therefore, PBL combined with the agricultural production plays an important role in cultivating students’ ability of integrating multi-disciplinary knowledge, students’ scientific thinking, and innovative research ability. Related Articles | Metrics

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Microbial mediated anaerobic ammonia oxidation coupled with different electron acceptors WANG Furong, WEI Manman Journal of Biology    2025, 42 (2): 91-.   DOI: 10.3969/j.issn.2095-1736.2025.02.091 Abstract （54）      PDF       Knowledge map    Anaerobic ammonia oxidation coupled with different electron acceptors is a series of novel biological denitrification approaches reported in recent years, which has not only attracted much attention in the field of wastewater biological treatment, but also played a crucial role in geochemical cycles of nitrogen, sulfur, iron, and manganese. This paper focused on four anaerobic ammonia oxidation processes coupled with different electron acceptors, including nitrite-dependent anaerobic ammonia oxidation, sulfate type anaerobic ammonia oxidation (Sulfammox), anaerobic ammonium oxidation coupled to Fe(Ⅲ) reduction (Feammox) and anaerobic ammonium oxidation mediated by MnO2(Mnammox). The reaction mechanisms and reaction equations were summarized and the phylogenetic diversity and metabolic characteristics of different anaerobic ammonia oxidizing bacteria were reviewed. The research progress and functional microorganisms of anaerobic ammonia oxidation processes coupled with different electron acceptors were elucidated. Finally, the application bottlenecks and the future development were discussed. The aim was to provide theoretical support for further in-depth research, and to provide a new perspective for promoting the development of anaerobic ammonia oxidation technology with different electron acceptors and the application in the field of wastewater denitrification. Related Articles | Metrics

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Research progress on prophylactic monoclonal antibody drugs for rabies post exposure prophylaxis WU Meng, ZHANG Hao, LIU Xueqin, TU Changchun, LIU Yan, GE Liangpeng Journal of Biology    2024, 41 (5): 100-.   DOI: 10.3969/j.issn.2095-1736.2024.05.100 Abstract （151）      PDF       Knowledge map    The current epidemic status, pathogenesis of rabies, the neutralizing antigen sites of RABV glycoprotein and the neutralization mechanism of the monoclonal antibodies against RABV infection were briefly described. The screening techniques, recognition sites, action mechanism and neutralization effects of almost all of the monoclonal antibodies against RABV which are currently available on the market or under development worldwide were also introduced. The paper further analyzed the problems existing in the application of the above mentioned monoclonal antibodies and the disadvantage of rabies immune globulin in the current post-exposure prophylaxis of RABV infection, including the limited blood supply, neutralization titers, the quality control and potential virus infection, which implies that the more attention should be paid to the development of full human monoclonal antibodies against RABV, especially for their neutralization efficiency and breadth. Taken together, the full human monoclonal antibodies drugs targeting the phylogroup Ⅱ/Ⅲ of glycoprotein and the cocktail targeting multiple antigenic sites are potential efficient strategy to the development of post-exposure prophylaxis of RABV infection. Related Articles | Metrics

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Effects of endophytic fungi expansion of Hordeum bogdanii on soil enzyme activity and nutrients HU Mengfei, CHEN Sheng, LIU Xiaozhen, TENG Tianxin, CHEN Shuihong Journal of Biology    2024, 41 (4): 57-.   DOI: 10.3969/j.issn.2095-1736.2024.04.057 Abstract （114）      PDF       Knowledge map    UsingHordeum bogdaniiseeds with endophytic fungi (E+) and without endophytic fungi (E－) as materials, seedlings were planted in greenhouses at first and then transplanted in fields with different salinity and alkalinity. The surface soils sampled in spring, summer and autumn, and spring samples as control groups, were used to determine the activities of catalase, urease, sucrase and alkaline phosphatase, as well as the contents of organic carbon, total nitrogen, total phosphorus, total potassium, available phosphorus and available potassium. The results showed that the contents of urease, sucrase, alkaline phosphatase, organic carbon, nitrogen, and potassium were the highest under moderate salt stress. With the increase of planting time ofH. bogdanii, the contents of catalase, carbon, nitrogen, phosphorus, potassium, and available potassium had no significant change. The contents of urease, sucrase, and available phosphorus increased, and the activity of alkaline phosphatase increased under medium salt. Moreover, the soil enzyme activity and the contents of carbon, nitrogen, phosphorus, potassium, available phosphorus, and available potassium in E+H. bogdaniiwere mostly higher than those in E－ under different salt levels. This indicated that endophytic fungi infection could improve the enzyme activity and soil nutrients of near plants by regulating them, laying a certain foundation for further research on the impact of endophytic fungi expansion on soil microbial diversity inH. bogdanii. Related Articles | Metrics

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Optimization of enhanced leaching copper with Acidithiobacillus thiooxidans from waste printed circuit boards using microwave #br# LIU Qian, LI Ruyan, LI Xiang, MA Changwen, TIAN Zhen, LIU Wenwen Journal of Biology    2024, 41 (3): 40-.   DOI: 10.3969/j.issn.2095-1736.2024.03.040 Abstract （139）      PDF       Knowledge map    In order to improve the leaching copper efficiency, microwave was used to enhance the leaching system ofA. thiooxidans. Plackett-Burman and Box-Behnken methods were used to optimize the enhanced bioleaching condition. Firstly, the significant factors identified by Plackett-Burman method were microwave power, irradiation time and leaching time. Secondly, the central values of above-mentioned three factors of Box-Behnken test were identified by the steepest ascent experiment, which were microwave power of 340 W, irradiation time of 3.60 min and leaching time of 6.80 d. And then, the experimental conditions of bioleaching copper withA. thiooxidanswere optimized using Box-Behnken method. The research showed that the microwave power, leaching time, the interactions of microwave power and leaching time plus irradiation time and leaching time had the significant influence on the bioleaching copper, whereas the effect of irradiation time was not significant. The optimal experimental condition predicted by model was as follows: microwave power 336.10 W, irradiation time 3.59 min and leaching time 6.82 d, and the predicted optimal value was 75.69%. The adjusted optimal condition was microwave power 336 W, irradiation time 3.60 min and leaching time 6.80 d, the experimental value was 75.41%±0.76%, indicating a high agreement between the predicted and experimental values. Related Articles | Metrics

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Study on the effect of different proportions of pyrite on the interaction between acidophilic microorganisms and stibnite WANG Yirong, CHEN Lu, LIU Hongchang, ZHOU Yuhang, NIE Zhenyuan, CHEN Yuxin, CHEN Hao, XIA Jinlan Journal of Biology    2024, 41 (3): 21-.   DOI: 10.3969/j.issn.2095-1736.2024.03.021 Abstract （139）      PDF       Knowledge map    This study focused on the acidophilic microbial dissolution process of stibnite and investigated the effect of different proportions of pyrite on the interaction between acidophilic microorganisms and stibnite. The results showed that the Proteobacteria had the highest abundance at the phylum level, it was the main stibnite-tolerant bacterium.Acidithiobacillushad the highest level of abundance at the genus level, which was the representative dominant genus in acid mine environments. Additionally, SEM-EDS results showed that for microbial action systems with Sb2S3∶FeS21∶1 and 1∶2 , more corrosion pits and fine particles were found on the surface of mineral residues, and for the microbial action system with Sb2S3∶FeS21∶1, the interfacial reaction between stibnite and pyrite was stronger. XRD and XPS results indicated that the addition of pyrite could promote the oxidative decomposition of stibnite and sulfur oxidation by acidophilic microorganisms. Tafel polarization curves and EIS impedance results showed that when Sb2S3∶FeS2was 1∶1, the microbial system had the highest corrosion current density and the smallest charge-transfer impedance. The above results were of great significance for understanding the migration, transformation, and environmental fate of stibnite in antimony mining areas, and further promoting the development of antimony pollution control technologies. Related Articles | Metrics

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Research progress on bioleaching of yttrium from rare earths #br# YAN Linlin, WANG Hailan, XIAO Chunqiao, FANG Yun Journal of Biology    2024, 41 (3): 61-.   DOI: 10.3969/j.issn.2095-1736.2024.03.061 Abstract （200）      PDF       Knowledge map    The occurrence and distribution of rare earth yttrium (Y) in nature, the main chemical extraction and biological extraction methods were reviewed, and the relationship between rare earth yttrium and microorganisms was discussed from the aspects of rare earth yttrium bioleaching (microbial adsorption, microbial metabolite leaching) and the effect of rare earth yttrium on microorganisms. The biological extraction of rare earth yttrium has broad prospects, but it still faces many problems, such as the lack of dominant leaching strains and the unclear mechanism of mineral-microbial interaction. On this basis, this paper further put forward some suggestions, such as establishing a high-throughput microbial screening method and rapid identification of functional strains, strengthening the interaction mechanism between microorganisms and yttrium at the molecular level and the selective leaching of yttrium by microorganisms, popularizing the biological leaching of microbial metabolites, and optimizing the in-situ extraction technology, hoping to provide reference for the design and development of yttrium resources in China. Related Articles | Metrics

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An initial exploration synergistic anti-aging effects and mechanisms of acetaminophen and N-acetylcysteine JIANG Lixiang, HAN Qiong, DUAN Jiaxin, LI Han, CAO Qian, HUANG Xinhe Journal of Biology    2024, 41 (3): 77-.   DOI: 10.3969/j.issn.2095-1736.2024.03.077 Abstract （158）      PDF       Knowledge map    Drug synergy is a promising new anti-aging strategy. Construction of the aging model induced by D-galactose in HUVEC cells was aimed to explore the potential synergistic anti-aging effect of acetylsalicylic acid and acetylcysteine, and to investigate the underlying mechanisms of their synergy in anti-aging. Network pharmacology was used to screen a group of small molecules with potential anti-aging activity. D-galactose-induced HUVEC cells was employed to detect the anti-aging activity of small molecules, and Chou-Talalay model was used to screen two small molecule combinations with synergistic anti-aging effects. Further investigation of the mechanisms underlying the anti-aging activity mediated by the selected small molecule combinations was conducted using biochemical and cell biological approaches. Results showed that acetaminophen acid and acetylcysteine were a highly effective pair of small molecules with anti-aging activity, which could produce synergistic effects in mediating anti-aging. The synergistic combination significantly reduced intracellular reactive oxygen species levels, effectively relieved cell cycle arrest induced by D-galactose, and significantly reduced the transcription levels of early inflammatory factors (IL-1β, COX-2). In addition, the synergistic combination significantly reduced the protein levels of cell senescence markers (p53, p16), as well as p-p38 and nuclear p65 protein levels, indicating that the acetylsalicylic acid and N-acetylcysteine synergistic combination may inhibit cell aging via the MAPK/NF-κB pathway. These results provide a theoretical basis for the further development and utilization of acetylsalicylic acid and N-acetylcysteine as a synergistic anti-aging combination. Related Articles | Metrics

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Study on the mechanism of a biofilm reactor based on microalgal bacterial consortia for piggery wastewater treatment CHEN Hengyuan, FANG Yezi, ZHENG Huabao, WANG Min Journal of Biology    2024, 41 (4): 65-.   DOI: 10.3969/j.issn.2095-1736.2024.04.065 Abstract （157）      PDF       Knowledge map    The bio-reactor is a key process and important limiting factor during the treatment of livestock and poultry wastewater through microalgal bacterial biofilm. In this paper, a biofilm reactor was designed for microalgal bacterial consortia to treat piggery wastewater, and the influent ammonia nitrogen mass concentration was adjusted to explore its impact on nutrients removal and microalgae biomass production. Moreover, scanning electron microscopy was used to observe the formation of microalgal bacterial biofilm on the carrier. High-throughput sequencing was also used for microbial community analysis. The aim of this study was to reveal the change regularities of nutrients removal and the response mechanism of microbial community of microalgal bacterial biofilm wastewater treatment with the fluctuation of influent ammonia. The results showed that the maximum daily removal rate of ammonia nitrogen could reach 35.60 mg/（L·d） when the influent ammonia nitrogen mass concentration was 200 mg/L, and the dominant bacterial genus wasPrevotella_ 9,Clostridium_ Sensu_ Stricto_ 1,AcinetobacterandBifidobacterium. Moreover, a large number of microalgal bacterial clusters were observed on carrier illustrating the formation of microalgal bacterial film. When the influent ammonia nitrogen mass concentration increased to 300 mg/L, the daily removal rate of ammonia nitrogen significantly increased reaching a maximum of 45.70 mg/（L·d）. However, the sustained high ammonia nitrogen load made a certain impact on the microalgal bacterial biofilm, while the dominant bacterial genera turned toComamonasandChristensenellaceae_ R-7_ Group. Related Articles | Metrics

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Extraction and antibacterial activity screening of soil microbes from saline-alkali soil of northern Ningxia MA Xiaoli, DING Fanfan, WEI Jiyuan, ZHOU Lerui, HU Yunqi, NAN Zedong, JIANG Zhibo Journal of Biology    2024, 41 (4): 50-.   DOI: 10.3969/j.issn.2095-1736.2024.04.050 Abstract （142）      PDF       Knowledge map    45 single colonies with certain morphological characteristics were isolated from saline-alkali soil in northern Ningxia, and the 16S rRNA sequence of the strains were sequenced and identified by genome extraction technology. Results showed that there were 16 strains of actinomycetes, including 13 strains of Streptomyces, 3 strains of Acidophilum. The experimental results of antibacterial activity exhibited that all 16 strains of actinomycetes had antibacterial activity, among which 12 strains of secondary metabolites had significant antibacterial activity againstProteus vulgaris, 12 strains of secondary metabolites had obvious inhibitory activity againstStaphylococcus aureus, and 9 strains of secondary metabolites had certain growth inhibitory activity againstCandida albicans. The researches demonstrated that the microbial strains isolated from saline-alkali soil in northern Ningxia had strong antibacterial or bacteriostatic activity. This study not only enriched the microbial resources in special habitats, but also laid a foundation for further isolation of antibacterial lead compounds. Related Articles | Metrics