Abstract: In this study, the tissue expression pattern of AGL13 was analyzed by qPCR, and the RNA interference vector was constructed to explore the molecular regulation mechanism of oil synthesis in seed of Brassica napus to increase the oil content. Bioinformatics analysis showed that AGL13 protein of Brossica napus has complete and typical MADS-box conserved domain. The overall shape of protein showed typical DNA nucleic acid binding structure. The AGL13 belonged to the MADS-box family transcription factor, and genetic relationship was the closest with AGL13 protein of Arabidopsis. The qPCR study found that AGL13 expression was very high from the flowering stage to the seed 45DAP (ays seed after 45 d flowering), was highest in the fruit clamp 15DAP. The RNA interference vector was successfully constructed in the experiment. The results showed that the AGL13 gene was strongly expressed in seed development and oil accumulation, which may play an important role in the seed development and oil synthesis. The successful construction of RNA interference vector would be favourable for obtaining the transgenic B. napus, and observing the seed development and oil accumulation of transgenic B. napus. Thus determining the AGL13 gene plays an important role in the seed development and oil synthesis.

Key words: Brassica napus, MADS-box, AGL13, RNAi, oil synthesis