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Table of Content

    18 April 2016, Volume 33 Issue 2 Previous Issue    Next Issue
     
    General strategy and innovative practices on rehabilitation and protection of Chao lake
    GAO Bin-you, YANG Li-xin, SONG Chao
    2016, 33 (2):  1. 
    Abstract ( 264 )   PDF   Save
     The treatment to the water pollution of Chao lake is the requirement of national develop,pment strategy in China. This paper introduced the four kinds of reasons such as the longtime polluter accumulation, longtime polluted water accumulation, gradually disappeared wetlands and swamp and higher content of phosphorus in lake bottom as inner phosphorus releasing source, resulting in the water of the lake polluted. The polluters from point and non-point source pollution to the lake water mainly contained phosphorus and nitrogen elements. Research showed that the heavy polluted area located in the northwest part of the lake, while the lighter in the southwest part. The polluter accumulated during flood season, and dissipated into the Yangtze River after flood. According to the strategy of source control, the eight projects like road and bridge construction, flood control, channel and rive regulation, ecological rehabilitation, ecological agriculture, sewage interception and tourism development. To protect the project launching well, the effective working mechanism and assurance system were created and used to guide the next phase project scientifically.
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    Distribution characteristics of benthic fauna and its response to the outbreak of cyanobacteria in Chaohu Lake
    2016, 33 (2):  8. 
    Abstract ( 248 )   PDF   Save
    Adoption of the method of field investigation, the recent algae bloom in Chaohu Lake outbreak status and Chaohu Lake benthic animals the temporal and spatial distribution were analyzed. The results showed that:algal blooms in the western half of the lake outbreak frequency is far higher than the eastern half of the lake, located in the South Feihe River Chaohu Lake in the northwest part is the outbreak of cyanobacteria accumulation area, the East will continue to decrease.Benthic animals showed the eastern half of the lake is in the western half of Lake much, and clam less snails become more and more, the distribution characteristics and algal blooms caused by ecological destruction. The sampling survey results reflect the western half of Zoobenthos in the lake and bloom outbreak frequency distributions were in the opposite in 2014. The eastern half of the lake due to the bloom outbreak frequency and low and benthic fauna distribution without obvious correlation.In recent years, Chaohu Lake has increased the intensity of governance and water quality has improved, but the distribution of benthic animals did not show a significant change in the aquatic environment, we should increase the intensity of ecological environment in Chaohu Lake.
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    Study on the effect of purification of eutrophic water of Tangxi River wetland aquatic plants
    Cui li-shan, Wu Ke, Wu jing-jing, Jin Jie
    2016, 33 (2):  13. 
    Abstract ( 347 )   PDF   Save
     
    Through the static hydroponic experiment study of Tangxi River Wetland 9 common aquatic plant growth in eutrophic water and the TN, TP, CODcr removal ability. The results showed that: (1) The aquatic plants could grow in the water, biomass growth ratio is the highest Shi-changpu 54.36%, the lowest is 39.14% of Hydrilla verticillata; (2) 9 kinds of plants on the removal rate of TN is the highest Shi-changpu 80.62%, the lowest is 56.16% water hyacinth; the removal rate of TP is the highest and lowest is 78.84% reeds, Hydrilla verticillata 43.78%; the removal rate of CODcr is the highest in Huang-changpu 81.60%, the lowest is 44.76% of Hydrilla verticillata. Preliminary thought the 9 plants can choose Huang-changpu, Shi-changpu, reed combination can make the water body to achieve better purification effect.
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    Influential factors of tailings ecological environment in Wangjiaao, Chaohu
    SONG Zao-ming, LI He, CHEN Jun,GONG Chen,BAI Yu-fang,WU Ke
    2016, 33 (2):  17. 
    Abstract ( 269 )   PDF   Save
    The basic properties of Wangjiaao Sulfur-Iron tailings near Chaohu, Hefei China, were analyzed through laboratory simulation of static/dynamic leaching experiment, simulated rainfall, temperature and rainfall acidity conditions. The influential factors of Sulfur-Iron tailingst ecological environment (heavy metals and acid waste water) were assayed. The results showed that concentrations of Cu, Cr, Cd, Mn, Zn, As and Pb were 9.40, 9.59, 0.04, 78.28,9.19, 22.56, 63.68 mg/kg, respectively. Tailing was mainly present in residual and oxidable fraction, followed by acid exchangeable and reducible. The impact on the ecological environment followed the order of Zn>As>Cu >Cd>Mn>Cr>Pb. The NAG and NAPP values were H2SO4 203.84 kg·t-1 and 86.79 kg·t-1, the results indicated that the tailings had high acid forming potential. Leaching experiments results indicated that under the condition of rainfall concentration, acid rain and high temperature were advantageous to the dissolution of heavy metals and acid waste water. The contaminants in the tailings had great influence on surrounding ecological environment.
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    EUTROPHICATION CURRENT SITUATION AND RESEARCH OF THE EAST-HALF OF Lake CHAOHU
    2016, 33 (2):  21. 
    Abstract ( 311 )   PDF   Save
    In order to figure out the current eutrophic state in east-half of lake chaohu -the tendency of eutrophication has aggravates gradually in recent years. The community structure of phytoplankton and water quality in east-half of lake chaohu was investigated. 119 speices of phytoplankton belonging to 8 phyla were identified, the most abundant was bacillariophyta, with 41 species, accounting for 34.45% of the total species; followed successively was chlorophyta, with 40 species, accounting for 33.61% of the whole species; the average density was about 47.36×105 cells/L, cyanobacteria, which was the richest phyla in the lake, aproximately 40.08×105 cells/L, accounting for 84.62% of the total phytoplankton. Result shows, the peak of phytoplankton density appear in summer, and relatively low in winter. The chlorophyll a content is height, and obvious change follow temperature. The waters of the eastern half of the chaohu lake total phosphorus and total nitrogen content obviously change with the seasons, after a year of monitoring, change of TP in the range of between 0.016-0.045mg/L, the overall average of 0.059mg /L, TN change in the range of between 0.164 -1.432mg/L, the overall average of 0.803 mg/L, the COD content is not high in the eastern half of the chaohu lake, the variation in the range of between 1.26-4.33mg/L, with an average of 2.795mg/L, transparency in the 2014 range between 0.100-0.280m. According to the nutritional status of each evaluation index calculation formula parameters and related weights and nutritional status were calculated in accordance with the comprehensive trophic state index grading standards, the eastern half of the chaohu lake eutrophication evaluation, from the evaluation results, the eastern half of the chaohu lake full nutritional type reached mesotrophic, and gradually worsening.
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    Hefei Binhu lake national forest park of plant configuration research
    Wu Jing-jing, Wu Ke, Chen Jun Sun Ya-yun, Deng Chao, Jin Jie
    2016, 33 (2):  26. 
    Abstract ( 289 )   PDF   Save
    In recent years, Chaohu eutrophication is serious, many problems are difficult to solve. Artificial wetland system technology can effectively reduce nitrogen, phosphorus, heavy metals and other nutrients from the source, fundamentally solve the serious water pollution caused by the outbreak of blue-green algae in Chaohu every year. In order to study the purification effect of wetland on the water quality of Chaohu, Taking Binhu Hefei national Forest Park as an example, study of wetland location, season, plant species distribution, population function and wetland ecological functions and so on. A total of 228 species belonging to 84 families and 179 genera. On the basis of this, the existing plant configuration of the park is analyzed and discussed.
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    Community structure of rotifer in relation to environmental factors in Shengjin Lake
    Chao Wang, Lan Wang,Zhongze Zhouv
    2016, 33 (2):  30. 
    Abstract ( 311 )   PDF   Save
     
    In this study, we investigated the community structure of rotifers and environmental factors in the upper Shengjin Lake from February to July of 2014.A total of 18 genera and 32 species of rotifers were identified during the survey, and the dominant species were Brachionus angularis, Asplanchna priodonta, Polyarthra trigla, and Schizocerca diversicornis.It ranged from 0.28μg/L to 30.39μg/L .The minimum and maximum of density of rotifers recorded in winter and autumn, respectively. From the result of Pearson correlations and canonical correspondence analysis, environmental factors such as water temperature,dissolved oxygen and
    pH that had a considerable effect on the rotifer community structure in the Shengjin Lake.
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    Analysis of relationship between DNA fingerprinting by RAPD and species of spring plankton in Hengshan reservoir
    ZHANG Qing-ke1, YANG Liang-jie1, SHENG Zhang1, LIU Tao2, FAN Chun-bo2, ZHU Jun-quan1
    2016, 33 (2):  34. 
    Abstract ( 233 )   PDF   Save
     The RAPD fingerprinting based on the DNA polymorphism of the plankton communities at 12 sampling points from 4 stations in Hengshan Reservoir, Zhejiang, was analyzed and the species were identified in April 2013. The relationship between topological structure of DNA fingerprinting and species was discussed by the cluster analysis. The results showed that 87 bands with the length ranging from 120 to 1700 bp were gained from 12 screened random primers, and the polymorphic rate was 87.4%. On average, 56.2 bands were observed from each station. A maximum number of 63 bands were observed from the lower level of station 1 and a minimum of 50 bands were observed from the middle level of station 4. 64 kinds of planktons were identified from 4 stations with the maximum number of 27 in the upper level of station 4 and the minimum of 16 in the upper level of station 3. 17-25 kinds of planktons were observed from other stations. Achnanthes sp., Ankistrodesmus convolutus, Chlorella vulgaris, Phormidium tenue and Tintinnidium sp. were all detected from 12 sampling points. The plankton communities from 12 sampling points can be divided into two types by systematic cluster analysis based on the species and RAPD. This study reveals a close relationship between the DNA fingerprinting structure of the plankton community and species in Hengshan Reservoir.
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    Effect of the ion exchange chromatography ligand density on the absorption behavior of proteins
    YANG Xiao-yan1,LI Wen-lu1,KONG Ying-jun2,WANG Ming-lin1,ZHANG Gui-feng2,SU Zhi-guo2
    2016, 33 (2):  39. 
    Abstract ( 307 )   PDF   Save
     The effect of ligand density on the adsorption behavior of proteins was investigated using dual polarization interferometry (DPI).The sensor chips of DPI were modified with 3-aminopropy-l-triethoxysilane (DAPTMS). The ligand densities on the modified chips were compared using X-ray photoelectron spectroscopy.Lysozyme, myoglobin, chymotrypsin, bovine serum albumin and immunoglobulin were used as model proteins. The adsorption behaviors of five proteins, such as adsorption amount, adsorption density and spatial structure, on the modified chips were characterized using DPI. It was found that with the increase of ligand density, the adsorption amount and density of proteins at the interface increase. Meanwhile, the initial adsorption rate grows. The spatial structure of proteins, especially immunoglobulin, was closely related with ligand density. The thickness of adsorption immunoglobulin layer was much smaller than the molecular diameter. The results indicated that the ligand density on the modified chip affected the amount, density and spatial structure of proteins on the interfaces.
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    The film-forming properties of transglutaminase-catalyzed gluten
    SUN Hong-yu, CUI Li, WANG Ping, WANG Qiang, FAN Xue-rong, LI Shuai
    2016, 33 (2):  44. 
    Abstract ( 218 )   PDF   Save
     
    A novel transglutaminase-catalyzed gluten reaction system was developed to prepare gluten films. The enzyme concentration, pH value of the solution, temperature and reaction time on the gluten films' mechanical properties were discussed. Meanwhile, the effects of enzyme-catalyzed reactions on the hydrophobicity and soluble stability of the gluten films were investigated. The tensile strength of the modified gluten films was improved by 16.8 % compared with that of the control films when the system conditions were as follows: 20 U/g gluten of TGase, pH 7.0 of the solution, 40℃ of reaction temperature and 2 h of reaction time. In addition, the soluble stability and hydrophobicity of the modified gluten films were improved.
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    Enzymatic grafting of catechin onto silk fibroins with tyrosinase and its antioxidant performance
    Qi Cheng-long, Hu Yi-yuan, Wang Ping, Wang Qiang, Fan Xue-rong
    2016, 33 (2):  49. 
    Abstract ( 264 )   PDF   Save
    Enzymatic modification of silk fibroin was carried out by using tyrosinase and a natural antioxidant of catechin via covalent reaction between catechin and primary amine groups of silk fibroins. Spectrophotometry was used to evaluate the efficacy of adsorption and grafting of catechin onto the fibroin surfaces. The parameters like color depth, UPF value, antioxidant ability (DPPH method) and mechanical behavior for the catechin-treated silk fabrics were investigated, respectively. The results indicated that catechin could be enzymatically oxidized and conversed into dark quinone products. The color depth and UPF value for the treated sample were higher than that of the control, while less strengthen changes were detected before and after enzymatic treatments. Furthermore, both catechin treatment and tyrosinase/catechin treatment improved the antioxidant ability of silk fabrics against DPPH radical, more satisfactory result was achieved for the sample based on the combined treatment with tyrosinase and catechin.
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    Study on optimization of SOD extraction conditions from earthworm
    Zhao Xuelan, Zhao Yu, Song Chengjun
    2016, 33 (2):  54. 
    Abstract ( 378 )   PDF   Save
    Superoxide dismutase (SOD) has been demonstrated to be an important antioxidant, which protects the cells exposed to the toxicity of oxygen and has displayed huge application value and development prospect. The phosphate buffer was used to extract SOD, the antioxidant component of earthwarm (Eisenia foetida). Four key extraction process parameters factors selected for single-factor tests were phosphate buffer pH value,Heat-treatment temperature,Ratio of solid to liquid, and degree of saturation((NH4)2SO4). Four factors and four levels of orthogonal experiment were used to determine the optimum factors levels above. Then activity of the SOD was measured by adjacent benzene three phenolic autoxidation method. Results indicated that the optimal levels of these parameters were as follows: phosphate buffer pH value 8.0, the heat treatment temperature 300C, ratio of solid to liquid 6, and degree of saturation((NH4)2SO4) 51%~70%. Under these conditions, the activity of the SOD was up to 2895.32U/g[FW]. This study provided a technical basis for the production of large scale production of SOD from earthworm.
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    Study on antimicrobial activityofendophytic fungi from Aloeveral.var.chinesis(haw.)
    LI Ling-ling, LUO He-chun, ZHANG Xian-shu
    2016, 33 (2):  58. 
    Abstract ( 291 )   PDF   Save
     
    Eight strains of endophytic fungi were isolated from the leaf and root of Aloeveral.var.chinesis(haw.), in which, five from the leaf and three from the root. The antimicrobial activity from endophytic fungi was investigated by using the agar diffusion method and the cylinder plate method with Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Saccharomyces cerevisiae as indicator microorganisms. The results showed that two of endophytic fungi which one was isolated from the leaf and the other from root had inhibitory activities to Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and the endophytic fungi from leaf showed antimicrobial activity to Saccharomyces cerevisiae. They were classified as Aspergillus Niger by their morphological features.
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    Inductive effect of methyl jasmonate on amaranthin accumulation in Amaranthus mangostanus L. seedlings
    JIN Hao, ZHU Chang-hua, XIA Kai, GAN Li-jun
    2016, 33 (2):  61. 
    Abstract ( 287 )   PDF   Save
     
    Amaranthin is one of the important plant secondary metabolites. Light can trigger the initiation of amaranthin biosynthesis in cotyledons of Amaranthus mangostanus~20 mg/L) induced amaranthin accumulation was dose-dependent both in light and darkness. When in darkness, 20 mg/L MeJA induced 10.91–fold amaranthin contents that of controls. While in light, presence of 20 mg/L MeJA induced 2.23-fold that of cotyledons exposed to light only. When used 4-leaf stage seedlings as material, MeJA (1.25~10 mg/L) could promote amaranthin accumulation both in cotyledons and leafs, stems of Amaranthus mangostanus /Lwas the best. But high concentrations of MeJA could inhibit growth of the seedlings and leaf expansion. The following experiments indicated that MeJA could induce amaranthin acculation by promoting the activity of tyrosinase. From above, MeJA could promote amaranthin accumulation both in seedlings of Amaranthus mangostanus L., but application of MeJA had inhibitory effect on plant growth and development.L. Effect of 10 mg L. In this paper, with Amaranthus mangostanus L. as the material, the effect of MeJA (Methyl Jasmonate) on amaranthin synthesis was exploreded. MeJA ( 0.02
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    Study of the ultrasonic assisted disinfection method to save pollution tissue culture seedling
    CHEN Zong-li, ZHANG Zhi-chan, HAN Ai-qin, BAI Yong, YANG Yang, ZHANG Le-yuan
    2016, 33 (2):  66. 
    Abstract ( 259 )   PDF   Save
    To obtain Zizyphus jujube mill, Jinnan 1, tissue culture seedling contaminated with bacteria, methods were studied by using the ethanol, mercuric chloride and ultrasonic as primarily test factors together with the random block design of experiment. The results showed that optimal combined disinfection method for saving pollution tissue culture seedling was as follow samples soaked in 70% ethanol for 30 s, then in 0.1% mercuric chloride for 4 min and treated with ultrasonic(28 HZ,25℃) for15 min. Compared with the conventional ethanol-mercury chloride chemical disinfection method, pollution rate of inoculation explants used with this method was reduced 68.41%, the survival rate increased by 75.72%, the proliferation degree increased by 4.41 time. This a new method of physical and chemical disinfection showed good feasibility.
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    Research on the effect of soaking treatment with γ-aminobutyric acid on potato germination
    XIAO Hui-yuan, ZHU Chang-hua, GAN Li-jun, XIA Kai
    2016, 33 (2):  71. 
    Abstract ( 226 )   PDF   Save

    In this study, potato (Kexin No. 13) was used as the material to study the effect of soaking application of γ- aminobutyric acid (0.2 to 5.0 mmol/L) on the potato germination. The changes of amylase activity, starch The results showed that appropriate amount of γ- aminobutyric acid may improve the germination of potato and dry matter accumulation of buds by elevating the activity of starch enzyme, promoting the decomposition of starch, increasing reducing sugar content, facilitating the plumule elongation and improving the dry and fresh weight of shoot and root. γ- Aminobutyric acid at the concentration of 1.0 mmol/L showed the optimal effect on potato germination. content, reducing sugar content, bud length, fresh and dry weight of shoot and root in seed potatoes were measured and determined in the experiment.

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      Studies on energy density and nutrient accumulation of the young Eriocheir sinensis living after the last winter before the first molting in the second year
    2016, 33 (2):  76. 
    Abstract ( 236 )   PDF   Save
    Different body-size young Chinese mitten crabs (Eriocheir sinensis), living from the end of the last winter to the beginning of the first molting in the second year, were fed with three different diets, the corn, the crusion carp meat, and the formulated diet. And the energy density of the whole crab, the content of total lipid and crude protein in the hepatopancreas and muscle were dentermined. The result showed that, the energy density of the whole crab rised firstly, and then declined to the same level with that before feeding. The energy density early in the feeding of formulated diet group was significantly higher than that of the other groups (P<0.05). The energy density of big body-size crabs was higher than that of small body-size, but the difference was not significant (P>0.05). And the energy density of the big body-size crab in the group fed with corn was significantly higher than that of the others (P<0.05). The content of total lipid and crude protein in the hepatopancreas in all groups showed no significant differences (P>0.05). The content of both total lipid and crude protein in the muscle of small body-size crabs declined significantly (P<0.05), while the total lipid of big body-size crabs showed no significant differences (P>0.05), but the content of crude protein rised significantly (P<0.05).
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    Studies on the histological structure on digestive system of young Cromileptes altivelis
    OU You-jun1, LIU Jiang-hua1,2, LI Jia-er1,WU Shui-qing1,2
    2016, 33 (2):  81. 
    Abstract ( 235 )   PDF   Save

    This paper dealt with the histological structure in the digestive system of young Cromileptes altivelis so as to provide morphological basis for understanding its development and growth characteristics and improving the survival rate in seedling production. Paraffin section and HE staining were used conduced to observe the tissue slice in the digestive tract of young fish. Results showed that mucous cells and taste buds were found both in the lips and tongue, goblet cells were not found in lips, but abundant in tongue; The mucosa layer of esophagus, esophagus was short and think, covered with wrinkles and folds with numerous mucous cells and goblet cells, taste buds were not found, muscle layer of esophagus were well developed which was striated muscle; The stomach consisted of cardiac,blind and pylorus. The account of mucosa layer’s folds:
    cardiac stomach > blind stomach> pylorus stomach, the average height of mucosa layer’s folds: blind stomach> cardiac stomach> pylorus stomach. Concave-shaped structure was observed in stomach without goblet cells be found, the gastric gland were well developed; he intestine was divided into foregut, midgut and hindgut. The number and average height of mucosa layer’s folds in intestine: foregut > midgut > hindgut, he striated border was obvious in intestine with numerous goblet cells and mucous cells; he results of observation in pyloric caeca were similar with that in intestine. Lobuli hepatis were obvious. The distribution of central vein was irregular. Hepatic sinusoid was abundant. The hepatic cell cords were obvious, hepatic artery and bile duct could be observed. The pancreas was dispersing around the liver. Langerhans' islet was oval cluster, the pancreatic acinuses were abundant, and the pancreatic cells could be observed obviously. In conclusion: histological structure of digestive system in young stage of C. altivelis is typical carnivorous, and associated with function, feeding habit and environment.

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    Study on innovation model of agricultural transgenic biological technology
    WANG Yu, SHEN Wen-xing
    2016, 33 (2):  86. 
    Abstract ( 184 )   PDF   Save

    This paper focus on the two major models of original variety innovation and derivative variety innovation, and analyzes existing circumstances and the situation of agricultural transgenic biological technology innovation in China. Based on 210 questionnaires from the transgenic biological technology researchers and managerial staff members in Jiangsu province, this paper used the Logit model for empirical study on the factors that influence the choice of innovation model of innovation organization. The results showed that the property of innovation organization, the level of intellectual property protection, R & D investment, R & D costs, market access had varying effect on it. On the basis of status analysis and the result of empirical analysis , this paper further analyzed the intrinsic motivation of the innovation mode choice, and tryed to find the good policies to help solve the contradictions of the innovation incentive and market interest of the original varieties innovation.

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    Progress in research on animal embryo formation and development of miRNA regulation
    FAN Wen-tao,ZHONG Yang-sheng,CHEN Fang-yan, LIN Hui-chao,LIN Jian-rong
    2016, 33 (2):  91. 
    Abstract ( 321 )   PDF   Save
     
    MicroRNA(miRNA)is a class of endogenous non-coding small RNA molecules that are between 20 and 24 bases, it widely exists in animals,plants and other livings and binds to the target gene-specific target gene mRNA to cause degradating or inhibiting protein translation. So it has a wide range of biological functions. The formation and development of embryo takes an important position on animal reproduction, evolution. Recent studies showed that miRNA had effect on animal reproduction, embryonic development. To explain the molecular mechanism of its regulation, this paper reviewed the research of miRNA in animal embryo formation and development.
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    Advanced progress in alpha-fetoprotein and hepatocarcinoma immunity
    ZHAO Qian1, WANG Xiao-ping2, XU Bing1, LIN Huan-ping1, ZHANG Ke-pei1
    2016, 33 (2):  95. 
    Abstract ( 324 )   PDF   Save
    Hepatocarcinoma is one of the most prevalent gastroenterological cancers in the world with less effective therapy. Excepting for its diagnosis in liver cancer, AFP has become the target for liver cancer immunotherapy. Although AFP’s immunogenicity is weak and can promote the immune escapes by inhibiting the function of dendritic cells, natural killer cells and T lymphocytes, AFP has drawn more attention in liver cancer immunotherapy. By in vitro modification, the immunogenicity and immune effect of AFP could be enhanced. The important role of AFP in development, progression and immunity of liver cancer lays a better basis in hepatocarcinoma immunotherapy.
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    The Progress of Glycosylation of Cadherins
    YANG Shu-feng, DENG Guo-ying, LIU Xin, SUN Wen-chang
    2016, 33 (2):  100. 
    Abstract ( 362 )   PDF   Save
     
    Cadherin superfamily is a class of glycoproteins involved in cell-cell adhesion and cell-matrix interaction. Their regulating expression during development plays a crucial role in the morphogenesis and patterning of tissues and organs. It is known that N- -glycosylation is existed in cadherins. Besides that, the modification of O-mannosyaltion was found recently in these molecules. In this review, the recent progress of N-glycosylation and O-mannosylation of cadhesins was summarized.
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    Research Advances on the Genetic Relationship of Rose Germplasm Resources
    LI Dong-li1, GAO Shu-min1, ZHAO Hui-en2, WANG Xiao-xu1
    2016, 33 (2):  103. 
    Abstract ( 280 )   PDF   Save
    The methods of research on relationship of rose germplasm resources included morphology, palynology, cytology, biochemical analysis and DNA molecular markers. With constant innovation and improvement of DNA molecular marker, it was becoming more and more important in the study of rose relationship .The analysis of relationship can provide reference for the parent selection in breeding of distant hybridization that was an important way for rose germplasm innovation. This paper reviewed the research progress on the genetic relationship of rose germplasm resources. Therefore, it can provide basis for exploring the origin, evolution, classification, genetic diversity analysis, and marker-assisted seletion of rose germplasm resources.
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    Study on medium optimization of Suillus bovinus under tissue culture conditions
    Luan Zhu-Qing, Liang Li-Kun, Gong Xue-Qin, Liu Chuan-Lin, You Cui-Rong#, Chu Yang
    2016, 33 (2):  106. 
    Abstract ( 263 )   PDF   Save
     
     A tissue culture experiment about the effect of different factors on the growth of Suillus bovinus. The result showed that carbon source, carbon concentration, MS concentration, activated carbon concentration and other factors had a significant effect on the growth of Suillus bovinus. The optimal carbon source is glucose and sucrose, the optimal concentration of glucose is 20g/L, the optimal MS concentration is 1/4MS, the optimal activated carbon concentration is 0.5g/L, the optimal plant growth regulator is 0.4~0.6mg/L NAA. The highest daily growth of mycelia is 2.45mm.
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    A preliminary study on culturing earthworms by fermentation of food-waste and mushroom-residue
    WU Jia-yun,ZHAO Zhi-yuan,LIU Ming,WEN An-xiang
    2016, 33 (2):  110. 
    Abstract ( 360 )   PDF   Save
    Based on analysis of growth index, the reproduction rate, the protein content of the body and the utility of the earthworm casts, the breeding effects of earthworms culturing by fermentation of food-waste and mushroom-residue were evaluated in this study. Compared with groups feeding on cow dung, the efficiency of weight gain, the protein content of the body and the utility of earthworm casts were higher those in the group feeding on fermentations mixed with equal proportions of food-waste and mushroom-residue. However, the reproduction rate of earthworms was lower in the latter group than in the former. The results indicated that the fermentation of food-waste and mushroom-residue can be used as an organic fertilizer.
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    The comparison on the biology science course setting in the University of China and Britain
    XIE li-fang, LIN hong-hui
    2016, 33 (2):  113. 
    Abstract ( 270 )   PDF   Save
     
    In this paper the biological sciences were used as an example. 11 domestic universities and 3 British universities such as Oxford university, York university and Sheffield university were investigated. The differences in the biological sciences curriculum were contrasted. The existing problems in the course of our colleges were pointed out and some suggestions were put forward for improvement, in order to benefit the development of our higher education.
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    Practices of stereoscopic teaching mode on bilingual curriculum of botany
    LI Da-hui, YUAN Yi
    2016, 33 (2):  116. 
    Abstract ( 231 )   PDF   Save
    Botany is one traditional subject of biology. Bilingual teaching on botany should be able not only to provide chances for students to practice biological English, but also to activate their motivation to learn plant biology via foreign language. In order to improve the quality of bilingual teaching on botany, further promote the innovation abilities of students and their comprehensive understanding on basic theories in plant biology, a stereoscopic teaching mode, integrated with teaching concepts, teaching contents, and teaching methodology, has been applied to the curriculum of botany.
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    Practice and suggestions of taxonomy teaching of angiosperm based on research teaching
    LUO Le, XU Xiao-ying, ZHANG Shun-cang, JIANG Jin-jin, JIN Yin-gen
    2016, 33 (2):  119. 
    Abstract ( 232 )   PDF   Save
     Botany research-based teaching is advantageous to the innovation ability training of the major in biology, planting and so on. Adopting the teaching mode of “informative interpretation-substantial observation-general summary”, the integration of independent learning, cooperative learning, research learning and matched evaluation system could be designed, and the effectiveness, entirety and all personnel schoolmates of research-based teaching also was operated. “Three dimensional teaching” dominated by a teacher may help a student to construct the knowledge system, to develop the wisdom and the potential, and to enhance the innovative thinking and ability.
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    Comparative study of different teaching methods  in microbiology teaching
    SHI Hui 1, CHEN Hai-yan 1, ZHANG Jun-hong 2
    2016, 33 (2):  122. 
    Abstract ( 221 )   PDF   Save
     
     In the textbook of microbiology course, the chapters are divided into three types such as easy to understand type, difficult to understand and comprehensive application of chapter type. The indexes like the learning interest, the learning efficiency, the self-learning ability, the comprehensive ability and the classroom capacity were evaluated with Lecture Based Learning(LBL), Problem Based Learning(PBL)[1] and Discussion Based Learning(DBL) separately. Result showed that the different teaching methods were suitable for different types of chapters. The LBL teaching method can be used in easy to understand chapter of teaching; PBL teaching method can be applied in easy to understand chapter teaching; DBL attached to LBL is more suitable for the teaching of comprehensive application of chapters.
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    The Exploration and Practice of Construction and Management Model of Biochemistry Opening Laboratory
    MA Hong-fei, LI Wei, JIANG Lin-shi, MA Hui-qiang, JIANG Hu-sheng
    2016, 33 (2):  125. 
    Abstract ( 254 )   PDF   Save
    Biochemistry experimental teaching is an important link to enhance the students’ theories knowledge, improve the students’ manipulative ability and overall qualities. To enhance the overall level of experiment teaching through the open laboratory construction is the main direction of the reform experiment teaching at the present stage. The importance of biochemistry opening laboratory construction has been set forth and the idea and measures for opening laboratory construction of Liaoning Shihua University have been introduced. The effect of initial practice has also been described and the further step to improve biochemistry opening laboratory construction has been proposed.
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