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生物学杂志

Table of Content

    18 February 2016, Volume 33 Issue 1 Previous Issue    Next Issue
     
    Cytotoxicity of ZnO Nanoparticles on HEK293 cells
    CHEN Yu-ying，HAN Hong-yan
    2016, 33 (1):  1. 
    Abstract ( 413 )   PDF   Save
     
     This experiment was to explore the effects of concentration and size of ZnO Nanoparticles (ZnO NPs) on HEK293 cells and research its mechanism of toxicity. In this experiment, we used three different sizes ZnO NPs which include 15nm-sized ZnO NPs, 30 nm-sized ZnO NPs, 90nm-sized ZnO NPs and normal ZnO to treat HEK293 cells. After 48 hours, we detected the cell proliferation by MTT test, then we made a cell cytometry to investigate the rate of apoptosis. Last, we analyzed the change of ROS level by kit. There was no noticeable changes on HEK293 cells treated with 15nm-sized ZnO NPs below 2.0μg/mL concentration. While above 4.0μg/mL, the proliferation of HEK293 cells was reduced significantly. However therate of apoptosis and the level of ROS increased obviously. With the decrease of the size of ZnO NPs, the rate of apoptosis and the level of ROS increased, but the proliferation of cell gradually reduced in HEK293 cells. In this experiment, the result suggest that cytotoxicity of ZnO NPs on HEK293 cells be related to its concentration and size. Increasing of ROS induced by ZnO NPs may be one important element of cytotoxicity.
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    Moleculor Cloning and Expession and Purification of Glutaminyl-tRNA Synthetase
    2016, 33 (1):  6. 
    Abstract ( 377 )   PDF   Save
     In order to study the structure and function of Glutaminyl-tRNA Synthetase,we synthesise the Glutaminyl-tRNA Synthetase and -tRNAGlngene, then the gene was subcloned into expression vector PET-28a-His-GlnRS and PIDTpSMART,separately , expessed Glutaminyl-tRNA Synthetase protein in BL21(DE3) by IPTG induction and we determined the activity of purify Glutaminyl-tRNA Synthetase Results showed that the Glutaminyl-tRNA Synthetase protein had been successfully expressed in E.coli. The substrate glutamine do not inhibit the activity of Glutaminyl-tRNA Synthetase.
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    Sequence Analvsis and Structure Study of FASLG Protein
    Mai Xiu-ying，ZhouMan，Li Ping*,XuLiu*
    2016, 33 (1):  9. 
    Abstract ( 317 )   PDF   Save
     
    Objective: To provide theoretical basis and experimental ways for follow-up study, analysising the sequence signature, structure and receptor binding sites of FAS protein. Method: the sequence of FASLG protein was downloaded from NCBI database, which is studied by ProtParam，ProtScale，SignalP 4.1，TMHMM Server v. 2.0，ClustalX，SWISS-MODEL and AutoDock. Result: FASLG has 281 amine acids, which is alkaline, unstable hydrophilic and transmembrane protein. FASLG has no signal peptide and receptor binding cites of FAS. The 3D structure of FASLG consisted by 74 α helices，20 B sheets, 56 Extending segments and 131 coils. The FASLG protein couples with its receptor protein of FAS by 15amino acids，then they will form the FAS/FASLG signaling pathways to induce cell apoptosis. Conclusion: The sequence signature, 3D structure and receptor binding cites are obtained, which provides a new direction of studying the FAS/FASLG signaling pathway and knowing the role of miR-21 and FASLG in colon cancer cells.
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    Expression、purification and bioactivity of recombinant human keratinocyte growth factor-1
    ZHOU Zhao1，FAN Qing-lin2,SONG Li-hua2
    2016, 33 (1):  13. 
    Abstract ( 398 )   PDF   Save
     
    Objectives:To express soluble recombinant human keratinocyte growth factor-1(rhKGF-1) in Escherichia coli and determine the bioactivity of rhKGF-1 to provide the basis for developing new drugs containing rhKGF-1. Methods:The sequence encoding ΔN23 rhKGF-1（the form of KGF-1 missing 23amino acids in N terminal） was synthesized and fused with SUMO through overlap PCR to obtain 6His-sumo-rhKGF-1 fusion gene，which was the subcloned into expression plasmid pET30a.The recombinant plasmid pET30a-6His-sumo-rhKGF-1 was then transfected to E.coli BL21(DE3) for expressing the fusion protein. Firstly ,the fusion protein was purified by Ni-NTA.Then,it was digested by Sumo Protease,and purified by Ni-NTA again to obtain purified ΔN23 rhKGF1. The ΔN23 rhKGF1 was used to promote proliferation of HaCat cells,then assay its bioactivity by CCK-8 methods.Results:Recombiant expression plasmid pET30a-6His-sumo-rhKGF1 was constructed correctly,the result of sequencing was the same as the expected.The fusion protein was expressed in E.coli BL21(DE3) in soluble form.After purification and SUMO Protease digestion,purified ΔN23 rhKGF-1 had been obtained.It could promote HaCat cells proliferation,the value of EC50 was 6.11ng/mL.
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    Bioassay of Beauveria bassiana against Lissohoptrus oryzophilus infected in rice
    WANG Peng1,2 SI Huai-Jun1 WU Jia-He1,2
    2016, 33 (1):  17. 
    Abstract ( 261 )   PDF   Save
     Beauveria sp is an elite bioengineering bacterium against insect pests for protecting crops. It is important to screen the high toxicity B. bassiana against Lissohoptrus oryzophilus (an invasion insect pest) for protection of rice produce. In this study, eight B. bassiana strains were employed to assay the toxicity against L. oryzophilus through feeding rice tissues. The results showed that all strains had various potential toxicities to L. oryzophilus, mortality rates of pest infected with various B. bassiana strains showed obvious difference with 44.3-88.3% at 15 post inoculation, of which, Bb-3 exhibited the highest toxicity. Additionally, the toxic effect of B. bassiana against L. oryzophilus was positively related with spore content applied. Furthermore, the L. oryzophilus infected with Bb-3 reduced the damage to rice tissue compared to the control including number of feeding scars and length of scar. Together, our data suggest that the identification of high toxic B. bassiana strains and high dose spore application are two key factors against L. oryzophilus for protecting rice produce.
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    The seasonal variations of ammonia-oxidizing archaea in two kinds of ponds of Portunus trituberculatus
    ZHANG Dengping, WEI Meng, QIU Qiongfen*, WANG Chunlin
    2016, 33 (1):  21. 
    Abstract ( 269 )   PDF   Save
     
     The dynamic changes and diversity of ammonia-oxidizing archaeal communities in two kind of ponds (M1: pond with sanded around and netted bottom, M2: traditional soil pond) of Portunus trituberculatus-exopalaemon carinicauda polyculture in different seasons were investigated by terminal restriction fragment length polymorphism (T-RFLP) analysis based on ammonia-oxidizing amoA gene. The results showed that ClusterNitrosopumilus was dominant in both M1 and M2. The community structure of ammonia-oxidizing archaea in aquaculture water changed dramatically during the breeding time. The diversity index and the evenness index of ammonia-oxidizing archaea were the lowest in August and September in both M1 and M2, which indicated that the environment was fragile in this period. RDA analysis showed that the distribution of ammonia-oxidizing archaea in pond water was greatly influenced by ammonium nitrogen in August and September. The result of T-RFLP showed that the community of ammonia-oxidizing archaea was different in M1 and M2 during the farming time and changed differently in each pond. Compared to M1, the community succession of ammonia-oxidizing archaea over time was larger in M2, indicated the community of ammonia-oxidizing archaea was more stable in M1. As such, the improvement of pond sediment could reduce the community shift of ammonia-oxidizing archaea by season.
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    Comparative study on fatty acid composition of Porphyra haitanensis atdifferent harvest periods in different culture areas

     
    Li Wei1, Omannisa?Mamat, Xu Ji-lin *, Luo Qi-jun, Li Yan, Yan Xiao-jun
    2016, 33 (1):  27. 
    Abstract ( 268 )   PDF   Save
     
     The total lipids of different strains of Porphyra haitanensis in different culture areas were extracted using Bligh-Dyer method after harvesting at different periods. Fatty acids were analyzed quantitatively and qualitatively by gas chromatography coupled with mass spectrometry (GC-MS) after esterification. The results indicated that polyunsaturated fatty acids (PUFA) dominated the highest proportion in the fatty acids composition of Porphyra haitanensis, followed by saturated fatty acids（SFA）in which C16:0 was the main fatty acid accounting for 26%-33% of total lipids, and monounsaturated fatty acids（MUFA）were the lowest, mainly for C18:1(n-9). C20:5(n-3), or EPA, was the highest in PUFA, of which the relative percent of the total fatty acids was 32%~46%. The same strains of Porphyra haitanensis harvested in the different periods, of which the relative percent of C16:0 was decreasing gradually with the increase of harvest times, while in PUFA the percent of EPA was increasing apparently and the C20:4（n-6） was decreasing gradually. The Porphyra haitanensis harvested in the same period in different culture areas showed that PUFA content in the Zhejiang sea area was higher than in the Fujian. As for the different strains of Porphyra haitanensis in the same culture areas, PUFA especially EPA of ZheDong-1 was slightly higher than ShenFu-2 when harvested the first time. No obvious differences were seen in the other periods. This study facilitates the qualification and cultivation of Porphyra haitanensis as well as food development.
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    The expression of 5 fatty acid metabolism -related genes in different growth rapeseed stages
    Liu Kai, Xiao Gang, Zhang Zhenqian, Guan Chunyun, Chen Sheyuan, Wu Xianmeng
    2016, 33 (1):  32. 
    Abstract ( 241 )   PDF   Save
     
    In order to understand the expression of fatty acid metabolism-related genes in rapeseed materials with different oleic acid content, in this study, the leaves of 7-day seedlings, 5-6 leaf stage and overwintering period, petals and the seeds of 20-35 days after pollination as materials, the expression of 5 differential genes （Bna0691280，Bna0421530，Bna0547440，Bna0799930 and Bna0764010）which obtained by transcriptome analysis of high oleic rapeseed near-isogenic lines were studied. The results showed that: in the leaves, the expression levels in high oleic rapeseed material were higher than the expression levels in the low oleic rapeseed. As for the expression in flowers, except Bna0547440 and Bna0764010, the expression levels of the other 3 genes in high oleic rapeseed material were higher than the expression in low oleic rapeseed. While the expression in the seeds of 20-35 days after pollination, the expression levels in the high-oleic rapeseed were lower than the expression in the low-oleic rapeseed except Bna0799930. The results of this study can be used for the breeding study of molecular mechanism of high oleic rapeseed.
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    Effect of water extractives from corn stover on ethanol fermentation
    1.College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; 2. Jiangsu Key Lab of Biomass-based Green Fuel and Chemicals, Nanjing 210037, China
    2016, 33 (1):  35. 
    Abstract ( 283 )   PDF   Save
      
    Water extractives were obtained by water extraction of corn stover. The effects of water extractives or its diluted acid hydrolyzate from corn stover on xylose fermentation to ethanol by Pichia stipitis and glucose fermentation to ethanol by Saccharomyces cerevisiae were investigated. The results showed that, compared to the control, there were some negative effect on xylose fermentation to ethanol by P. stipitis when the water extractives were added to the medium. The degree of inhibition increased with the increase of water extractives, but not obvious. The sugar utilization ratio increased when the fermentation time increased to 48 h, however, the ethanol yield decreased (less than 80 %). There was little negative effect on glucose fermentation to ethanol by S. cerevisiae when the water extractives were added to the medium. When the diluted acid hydrolyzate of water extractives was added to the medium, the fermentation time of xylose to ethanol by P. stipitis extended from 24 h to 48 h, and the ethanol yield decreased while the sugar utilization ratio was the same. However, the diluted acid hydrolyzate of water extractives still had little negative effect on glucose fermentation to ethanol by S. cerevisiae. Therefore, P. stipitis was more sensitive than S. cerevisiae to the inhibitors existed in the water extractives and its diluted acid hydrolyzate. Water extraction before corn stover pretreatment could reduce the inhibitors produce; thereby improved the ethanol fermentability by yeasts. Finally, it provides reference for real commercial production of fuel ethanol.
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     Molecular phylogenetic analysis of floating Sargassum horneri associated with green tides in coastal area of Qingdao
    Chen Jun①②§, Wang Yin-chu①②§, Yu Qiurong③, Bi Yan-hui④, He Pei-min④, Liu Zhengyi①, Qin Song①*
    2016, 33 (1):  39. 
    Abstract ( 282 )   PDF   Save
     
    In the Yellow Sea, large-scale green tides have broken out consecutively for eight years from 2007 to 2014. However, a small scale of floating brown algae appeared at the same time in coastal area of Shandong Peninsula, which gave more pressures including environmental impacts and economic losses. The 5.8S rDNA-ITS spacer regions of samples collected during the green tide were sequenced and analyzed. Multiple sequence alignment and phylogenetic analysis were performed to reveal the affinitybetween a Qingdao sample and four reported Sargassum samples. Our results indicated that the genetic identity of the algae from the green tide was of 99% similarity with Sargassum horneri from the coastal area of Zhejiang province and Jiangling, Korea. Phylogenetic analysis revealed that the floating sample clustered with Sargassum horneri closely, which proved the floating brown algae in Qingdao accompanied with green tides, was Sargassum horneri.     
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    The leaf anatomical structure characteristics of four greening trees from urban in Xi’an under different air environment
    LIU Xiong-fei, SHI Hui, ZAN Li, WANG Yu-tao, WANG Hui-xia
    2016, 33 (1):  43. 
    Abstract ( 241 )   PDF   Save
     
    The leaf anatomical structure of four greening plants such as Ligustrum lucidum Ait.，Ligustrum quihoui Carr. , Prunus ceraifera Euonymus japonicas Thunb. were researched by frozen section methods. The leaves were anatomically observed, and indexes including the thickness of upper and lower cuticle, the thickness of epidermal cell, palisade tissue thickness, spongy tissue thickness and leaf thickness were measured. At the same time, the ratio of palisade tissue to spongy tissue(P/S), the ratio of palisade tissue to leaf thickness (CTR), the ratio of spongy tissue to leaf thickness(SR) were statistically analyzed. The results are as follows: (1)Leaf anatomical structure indexes among the sampling points exhibit different degrees of difference, especially the palisade tissue, the leaf thickness and the ratio of palisade tissue to leaf thickness; (2)The Correlation analysis showed that the mesophyll tissue，including palisade tissue and spongy tissue, make a significant contribution to the leaf thickness; It was also indicated that the upper cuticle had a significantly positive correlation with palisade tissue thickness, and a significantly negative correlation with spongy tissue thickness. (3)The P/S have largest coefficient variation and plasticity index among all anatomical structures, which means the P/S is more easily to make changes for environment. The coefficient variation and plasticity index of Prunus cerasifera cv. Pissardii were respectively 21.9% and 1.0218, which is the largest one in four species. It inferred that Prunus cerasifera cv. Pissardii maybe the most variable tree species of those greening plants in different environment.    cv. Pissardii.and
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    Seasonal and spatial variation of chlorophyll-a concentration in Shengjin Lake
    Lan Wang1, Chao Wang1, Xiuxia Zhao2, Zhongze Zhou1*
    2016, 33 (1):  49. 
    Abstract ( 289 )   PDF   Save
     
    Seasonal and spatial variation of chlorophyll-a concentration and its relationships with the main environmental factors in Shengjin Lake in January, April, August and November of 2013 were investigated. Results showed that the obvious
    seasonal variations were observed in chlorophyll-a concentration in Shengjin Lake. The concentration of chlorophyll-a ranged from 0.28μg/L to 30.39μg/L with an average value of 6.83± 5.60μg/L. The minimum and maximum of chlorophyll-a concentration were recorded in winter and autumn, respectively. The chlorophyll-a concentrations were relatively lower in winter and spring while much higher in summer and autumn. It also was found to have significant spatial heterogenity in chlorophyll-a concentration. The chlorophyll-a concentrations were the highest in the lower lake of the Shengjin Lake, followed by the middle and the upper lake. Pearson correlation analyses and Principal Component Analysis (PCA) revealed that the chlorophyll-a concentration in Shengjin Lake showed a significant correlation with water temperature, dissolved oxygen, and turbidity, while correlated with total phosphorus.
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    Effect of Amino Acid Composition in Collagen Peptides on the Intestinal Adsorption in Mice
    CAO Wei1,2, KONG Ying-jun2, KANG Ji-yao2, Sun Kun1,2, ZHA Sheng-hua2, ZHANG Gui-feng2*, WANG Ming-lin1*
    2016, 33 (1):  53. 
    Abstract ( 343 )   PDF   Save
     
    Effect of amino acid composition in collagen peptides on the intestinal adsorption in Mice was investigated. Collagen peptides (CPO), with mean MW 1kDa, were treated using cation and anion exchange chromatography respectively, and the eluted peptides were named as CPC and CPA respectively. The amino acid compositions in CPC and CPA were determined. The intestinal absorption of peptides by mice was determined by detecting plasma hydroxyproline (Hyp) and di/tri-peptide after intragastric infusion. The result indicated the Arg and Lys content in CPC were 12.2% and 7.4% higher than that in CPO, and the Arg and Lys content CPA were 18.3% and 26.5% less than that in CP“°C. More Arg/Lys-containing peptides were found in CPC than that in CPA by HPLC-MS analysis. The result of animal test indicated that the Hyp content in mice plasma after intragastric infusion of CPC is 1.39 times higher than that of CPA. Oligopeptides, Ser-Hyp-Gly, Pro-Hyp-Gly and Leu-Hyp, were found in mice plasma. The plasma concentration of three oligopeptides were 2.1, 1.48 and 1.51 higher after infusion of CPC compared to CPA. This research suggests that the amino acid composition is a key factor affecting peptide adsorption in intestine.
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    Seasonal variations of thermomoneutral zone and evaporative water loss were measured in Apodemus chevrieri
    ZHU Wan-Long1,# SUN Shu-Ran1,# CHEN Jin-long2 CAI Jin-hong1 ZHANG Hao1 MENG Li-Hua1 WANG Zheng-kun1,*
    2016, 33 (1):  57. 
    Abstract ( 514 )   PDF   Save
     
    In the present study, seasonal changes of thermomoneutral zone (TNZ) and evaporative water loss (EWL) were measured in Apodemus chevrieri. The results showed that TNZ was 20～27.5℃ in winter, 22.5～27.5℃ in summer, respectively. Body mass, body temperature (Tb), EWL, evaporative water loss/metabolic rate (EWL/RMR) and evaporative heat loss/total heat production (EHL/HP) were significant higher in summer than that in winter, but basic metabolic rate and metabolic water production/evaporative water loss （MWP/EWL）were lower in summer than that in winter. All of the results suggested that the shift of the lower critical temperature of TNZ is possibly associated with the rate of metabolism and ambient temperature (Ta). The upper critical temperature of TNZ may be independent of the changes in Ta. The changes of lower critical temperature of TNZ are an important strategy in adaptation to variations of Ta in A. chevrieri. Moreover, it decreased EWL and EHL/HP to cope with the cold and dry environments in winter.
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    Resolving Metazoan Phyla Divergence Times
    During Cambrian Explosion by COX I Protein molecules
    Li Kequn
    2016, 33 (1):  62. 
    Abstract ( 294 )   PDF   Save
     
    By using the equation for resolving absolute evolutionary rates of biological molecules provided by author and the classic “molecular clock” Cytochrome oxidase subunit I (COX I) protein molecules, the metazoan phyla divergence times during Cambrian explosion were obtained. These results show that Protostomia and Deuterostomia separated at 560 million years (myrs ) ago. The phyla divergence times of four major deuterstomia: Cephalochordata, Urochordata, Hemichordata and Echinodermata are 536 myrs ago, 540myrs ago, 548myrs ago and 545myrs ago respectively. These divergence times agree well with fossil records, and are obviously better than those obtained by other current molecule clock methods. Our method may provide a new way to understandorigins and evolution of creatures.
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    Molecular genetic research progress on autism spectrum disorders
    WANG Ya-liang, MA Yuan-yuan, BAI Xue, XING Wan-jin
    2016, 33 (1):  68. 
    Abstract ( 478 )   PDF   Save
     
     Autism spectrum disorders (ASDs) are a complex neurodevelopmental disorder that display restricted interests, repetitive behavior, impairments in language and communication, and an inability to engage in social interactions. The causes of the ASDs remain unknown for the majority of individuals with ASD. ASD is among the most heritable disorders. Family studies indicate that up to 25% of cases can be identified as a genetic cause. Chromosomal rearrangements as well as rare and de novo copy-number variants (CNVs) are present in ∼10–20% of individuals with ASD, compared with 1–2% in the general population and/or unaffected siblings. Evidences show the single-nucleotide polymorphisms (SNPs) seem to contribute to ASD susceptibility. These findings, coupled with genome sequencing data may unveil the genes implicated in ASDs. This paper summarizes the results of molecular genetic research on autism and mainly introduces the genetic risk factors such as CNVs, SNPs and candidate genes for autism spectrum disorders to provide relevant information.
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    General situation and Prospect of horizontal transfer of eukaryotic transposons
    ZHANG Hua-Hao，ZHANG Xiao-Gu
    2016, 33 (1):  73. 
    Abstract ( 525 )   PDF   Save
     
    Horizontal gene transfer is widespread in prokaryotes, but in multicellular eukaryotes these cases mainly involved in the transposon horizontal transfer. A large number of genome sequencing showed that transposons are an important component of eukaryotic genomes. The most important characteristic of transposon distinguishing from genes is able to mobile and amplify in the host genome, which make them more prone to horizontal transfer. Horizontal transfer is an important way which would allow the element to evade a seemingly inevitable vertical extinction in its original host lineage resulting from genetic drift, natural selection or mutational inactivation. In addition, horizontal transposon transfer in eukaryotic genomes has been a major force propelling genomic variation and biological innovation. Here，this paper would comprehensively summary the methods of identifying horizontal transposon transfer, the transfer mechanisms of transposons, the transfer bias and its contribution in the origin of new genes. Finally，we also discusses the urgency, necessity and importance of studying eukaryotic horizontal transposon transfer from the new perspective of the whole-genome wide.
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    Research progress of deoxynivalenol toxicity
    XU Wei, GENG Fangfang, FAN Mengxue, GONG Jiajie, LIN Genxiang, LI Yu, WU Jinjie, WANG Xichun*
    2016, 33 (1):  78. 
    Abstract ( 405 )   PDF   Save
    Deoxynivalenol (DON) is also called vomitoxin (VT) which is a mycotoxin produced by some Fusarium, and widely exists in corn, barley, wheat, and other agricultural product and feedstuff. DON has a wide toxic effect on human and different species of animals. DON can cause the apoptosis of various cells, and produce toxic effects on the body system, such as the neurotoxicity, reproductive toxicity, immunotoxicity, carcinogenesis, misshapen effect and mutagenesis etc. This paper reports the physical and chemical properties, contamination, toxic effects and the outlook of the DON, to provide theoretical basis for the study of mycotoxin toxicity.
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    Communication strategy of frogs in noisy environment
    Zhang Fang
    2016, 33 (1):  82. 
    Abstract ( 284 )   PDF   Save
     
    Acoustic communication play a vital role regulating the survival and reproduction in frogs. There were many environment noise including abiotic and biotic noise in their habitat, which would mask their acoustical signal and make the signal detection and recognition more difficulty. For the communication to be effective, senders must emit signals that are conspicuous and distinct from other background noise. Usually, frogs in noise habitat would use different strategies to avoid masking and evolved a certain adaptive mechanism to avoid masking by sounds in the ambience, which include long-term adaptation and short-term adaptation. The current article will focus on the strategies of acoustical communication of frogs in noise ambient and the results will benefit to the understanding for readers how frogs adapt the noisy environment and communicate effectively.
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    Microbial leaching technology and its application in the exploitation of the tailings
    Feng Guang-zhi＊ Shi Yu Shu Yu-feng
    2016, 33 (1):  92. 
    Abstract ( 335 )   PDF   Save
     
    This paper outlines the development status of microbial leaching technology, describes the status of microbial leaching technology in detail, especially its application in the exploitation of the tailings, including cultivation of excellent strains, the main effecting factors on bioleaching and leaching process. The development prospects of bioleaching technology of tailings are forecasted, screening of bacterium for tailings bioleaching, study of effecting factors on bioleaching, the exploitation of in-situ leaching technology for tailings is the key about tailings resource utilization.
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    Site-direct coupling of lysozyme on sepharose microspheres using click chemistry
    2016, 33 (1):  104. 
    Abstract ( 403 )   PDF   Save
     A method for site-direct coupling of protein on sepharose microspheres was developed using click chemistry with lysozyme as model protein. Sepharose microspheres was activated using 1,4-butanediol diglycidyl ether. Alkynyl group was introduced by reaction of the epoxy group with 4-ethynlaniline. Azido-decanoic acid, synthesized by reaction with sodium azide and 10-bromodecanoic acid, was used to modify lysozyme by EDC/NHS method. The modified lysozymes was separated using chromatographic method and three isomers with different modification sites were obtained. Three azido-decanoic-linking sites in three isomers were identified as Lys1, Lys33 and Lys96 respectively using HPLC-MS. The azido-decanoic acid-modified lysozymes with Lys96 as linking site was separated and further reacted with 4-ethynlaniline-modified sepharose microspheres. The tryptic digest of the coupled lysozyme was analyzed using HPLC-MS, and the Gln74-Lys96 was disappear, indicating that link site of lysozyme on sepharose microspheres was Lys96. Thus, the method for site-direct coupling of lysozyme on sepharose microspheres using click chemistry was confirmed.
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    Effects of Three Culture Media on the Growth of Chlorella and Its Application in Culture of Freshwater Rotifers
    Li Fei, Guo Jianlin, Zhang Aiju, Liu Shili,Wang Yuchen, Huang Xianming, Zhang Yufei
    2016, 33 (1):  109. 
    Abstract ( 747 )   PDF   Save
     
    SE, BG11 and CF(compound fertilizer) were used to culture Chlorella, to study their effects on growth of Chlorella, and the effects of five different concentration of CF were studied on the culture of freshwater rotifers. The results indicated that all the three culture media could obtain good culture in Chlorella. In 1 and 7 days culture, BG11 was the best, CF was followed, SE was the last; in 16, 20 and 24 days culture, BG11 was the best, SE was followed, CF was the last; the differences were significant. 60mg/L was the best in the five concentration of CF in culture of Chlorella and freshwater rotifers, the concentration of rotifers was 324.67±37.22ind/ml,and it was significantly different from other groups.
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    a novel operation method for Biomed Computing Platform
    2016, 33 (1):  112. 
    Abstract ( 277 )   PDF   Save
     
     In this paper, we use open source Wiki system to create a Wiki-based operation method for our Biomed computing platform . We deeply analyse the Wiki system and pointed out our design concept according to our operation practices. We design some useful modules for our regular operation work and accumulate some operation-related knowledge based on this method. In the last few years, the method solves many practice problems in our work and formed a valued best practice for this field .
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    Research and Practice on Module Reformation of Biological Engineering and Technology
    2016, 33 (1):  116. 
    Abstract ( 256 )   PDF   Save
      
    According to the actual condition of the enterprise demand of ability of biological engineering graduates, the existing problems of traditional teaching content and teaching mode were analyzed. At the same time, the reform situation of biological engineering and technology module was introduced.  The lecture about production process of biological products and the cultivation about ability of practice and comprehensive application were presented for applied colleges.
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    Educational Practice Research of Garden Technology Professional (Undergraduate) in Colleges and Universities
    Duan Yanhong1*, Wen Bo2
    2016, 33 (1):  119. 
    Abstract ( 246 )   PDF   Save
     
    With the progress and development of human society, the role of garden technology professional is more and more significant. Garden technology of the higher school has also gradually become a popular professional of modern Chinese society, but the talent cultivation far cannot satisfy the needs of social development. Garden technology specialized educational reform practice also is especially obvious and important, the organic combination of production, is an important direction for the future development of garden technology undergraduate. "On the basis of the theory, for the purpose of service, market-oriented, with skills as the core" of the new ideas of teaching, through the practice, quite productive, to improve students' ability, can improve the quality of talent.
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    Practice and experience of website online question answering of Biochemistry
    2016, 33 (1):  123. 
    Abstract ( 329 )   PDF   Save
     
     Biochemistry is an important basic course for undergraduate majors of life science. Question answering is necessary for course teaching. However, traditional way for questions answering is restricted by many factors such as time, place. With the popularization of network and the development of education technology, the website construction for professional courses has beening paid more and more attention in the universities of china. Constructed a question answering platform on which teacher-student interaction can conduct in the course website of the Biochemistry broke through these restriction factors and improved teaching effect. This paper gives a brief description of the problem Practice and experience of website online question answering of the Biochemistry.
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    Discussion on some problems in "Chinese terms in Genetics (second edition)"
    CAI Shao-jing
    2016, 33 (1):  126. 
    Abstract ( 299 )   PDF   Save
     
    The terms in “classical genetics” of" Chinese terms in Genetics (second edition) "were discussed.  The deficiencies of the definition and comment of these terms on the content, grammar and language were pointed out　and the amendments were proposed. It aims to suggest authors and publishers to pay attention to these ideas discussed in this article to improve the qualityof "Chinese terms in Genetics (third edition))" significantly.请键入文字或网站地址，或者上传文档。
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