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Effect of water extractives from corn stover on ethanol fermentation

  

  1. 1.College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; 2. Jiangsu Key Lab of Biomass-based Green Fuel and Chemicals, Nanjing 210037, China
  • Online:2016-02-18 Published:2016-02-18

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Abstract:   

Water extractives were obtained by water extraction of corn stover. The effects of water extractives or its diluted acid hydrolyzate from corn stover on xylose fermentation to ethanol by Pichia stipitis and glucose fermentation to ethanol by Saccharomyces cerevisiae were investigated. The results showed that, compared to the control, there were some negative effect on xylose fermentation to ethanol by P. stipitis when the water extractives were added to the medium. The degree of inhibition increased with the increase of water extractives, but not obvious. The sugar utilization ratio increased when the fermentation time increased to 48 h, however, the ethanol yield decreased (less than 80 %). There was little negative effect on glucose fermentation to ethanol by S. cerevisiae when the water extractives were added to the medium. When the diluted acid hydrolyzate of water extractives was added to the medium, the fermentation time of xylose to ethanol by P. stipitis extended from 24 h to 48 h, and the ethanol yield decreased while the sugar utilization ratio was the same. However, the diluted acid hydrolyzate of water extractives still had little negative effect on glucose fermentation to ethanol by S. cerevisiae. Therefore, P. stipitis was more sensitive than S. cerevisiae to the inhibitors existed in the water extractives and its diluted acid hydrolyzate. Water extraction before corn stover pretreatment could reduce the inhibitors produce; thereby improved the ethanol fermentability by yeasts. Finally, it provides reference for real commercial production of fuel ethanol.