生物学杂志

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Study on medium optimization of Suillus bovinus under tissue culture conditions

  

  1. College of Life Science, Yantai University, Yantai, Shandong
  • Online:2016-04-18 Published:2016-04-18

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Abstract:  

 A tissue culture experiment about the effect of different factors on the growth of Suillus bovinus. The result showed that carbon source, carbon concentration, MS concentration, activated carbon concentration and other factors had a significant effect on the growth of Suillus bovinus. The optimal carbon source is glucose and sucrose, the optimal concentration of glucose is 20g/L, the optimal MS concentration is 1/4MS, the optimal activated carbon concentration is 0.5g/L, the optimal plant growth regulator is 0.4~0.6mg/L NAA. The highest daily growth of mycelia is 2.45mm.

Key words: Suillus bovinus, tissue culture, carbon source,  MS, plant growth regulator