Table of Content

18 February 2018, Volume 35 Issue 1 Previous Issue    Next Issue

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Characterization of a brittle culm mutant w7bc5 in japonica rice LU He-wei,LIU Bin-mei,TAO Liang-zhi,YE Ya-feng,WU Zhen-yu,FAN Shuang,WU Yue-jin,WANG Yu 2018, 35 (1):  1.  Abstract ( 316 )   PDF   Save  A brittle culm mutant w7bc5 was obtained from japonica rice wyj7 by heavy ion irradiation. The fertility and thousand-seed weight of w7bc5 mutant reduced, while other agronomic characters changed slightly. The w7bc5 mutant showed 29.8% less breaking-resistant strength and 26.7% less cellulose content compared to wild type plant, while hemi-cellulose increased evidently, no significant difference has been found in contents of lignin and SiO2. Results from analyzing soluble sugar contents of w7bc5 and wild type showed a 12.8 mg/g difference during heading stage, which further extended to 62.9 mg/g.  Related Articles | Metrics

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Molecular cloning, sequence characterization and expression analysis of HSP90β from ayu (Plecoglossus altivelis) MO Li-bin, LI Chang-hong, CHEN Jiong 2018, 35 (1):  5.  Abstract ( 272 )   PDF   Save Heat shock proteins (HSPs) are one type of molecular chaperones, and have a wide range of biological activities. The full-length cDNA sequence of ayu HSP90β gene (PaHSP90β) was obtained from the liver tissue cDNA library of ayu (Plecoglossus altivelis). The cDNA sequence of PaHSP90β was 2726 nucleotides, containing a 2175 bp open reading frame that encoded a protein of 724 amino acids with a deduced molecular weight (MW) of 83.30 ku and a isoelectric point (pI) of 4.83. Multiple sequence alignment of complete amino acid sequences showed that PaHSP90β contained five conserved HSP90 family signatures, and a typical HATPase (histidine kinase-like ATPases) domain, which was located at positions 34 to 188. PaHSP90β shared the highest amino acid sequence identity (95.8%) with that of European whitefish (Coregonus lavaretus). Quantitative real-time PCR (qPCR) analysis showed that PaHSP90β mRNA had the highest expressional level in ayu liver, followed by the spleen. Upon Cd exposure, PaHSP90β transcripts were significantly up-regulated in liver, spleen, kidney, intestine and gill of ayu. After the salinity stress, PaHSP90β mRNA expression was significantly up-regulated in all the tissues except the intestine. In summary, PaHSP90β had a close relationship with heavy metal and salinity stress, and may be involved in physiological processes against environmental stresses in fish.     Related Articles | Metrics

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Analysis of microorganism in infant feces using HPLC-MS and MALDI-TOF MS GAO Li-juan, GAO Jian-ping, MA KAI, LI Sai-na,ZHANG Gui-feng 2018, 35 (1):  11.  Abstract ( 324 )   PDF   Save Microorganism in infant feces was analyzed using matrix-assisted laser desorption/ ionization time of flight mass spectrometry ( MALDI-TOF MS ) and high performance liquid chromatography mass spectrometry (HPLC-MS). Seventy samples from 0 to 3 years old infants were pretreated and analyzed by the two methods, and the results were compared. MALDI-TOF mass spectra indicated that ions observed increased with the infants′ age, more microorganisms were detected in 2-3 years old infant feces than that from 0-2 years old. HPLC-MS results indicated that the main microorganism in 0-1years old infant feces were Escherichia coli（E. coli）, Streptomycetaceae, yeast, monilia albican and streptococcus. The high abundance microorganism in 1-2 years old infant feces were red hair fungus bacteria, E. coli, monilia albican, yeast and and 2-3 years old infant feces were bifidobacterium, streptococcus, candida and E.coli. Related Articles | Metrics

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Characterization and expression analysis of HSP17.8 protein in Sophora flavescens Ait LIAO Yi,ZHAO De-rui,HU Xue-rui, WU Jia-wen 2018, 35 (1):  15.  Abstract ( 286 )   PDF   Save The hsp gene fragment was amplified by polymerase chain reaction (PCR) from the leaves of Sophora Flavescens Ait, and then it was successfully ligated to the prokaryotic expression vector pET22b(+) for protein expression. The expression of hsp was induced with different concentrations of IPTG at different temperature. The coding region of the hsp gene was 498 bp, encoding for a protein of 166 amino acids with an estimated molecular mass of 17.8 ku (HSP17.8). The physicochemical properties, phylogenetic tree, secondary structure and 3D model of HSP17.8 were analyzed by bioinformatics software. The results showed that HSP17.8 was a stable hydrophilic protein with multiple N-myristoylation sites, phosphorylation sites and N-glycosylation sites. The protein had the closest relationship with HSP from Lupinus angustifolius. The 3D structure of HSP17.8 folds into a helix-turn-helix motif on a five-stranded beta-sheet scaffold arranged in the order β1-α1-β2-α2-β3-α3-β4-α4-β5. The hsp17.8 was cloned from Sophorae flavescentis Ait and expressed in vitro for the first time. The study of physicochemical properties, phylogenetic relationships and structure characteristics of HSP17.8 provided a theoretical foundation for the further structural and functional researches of HSP17.8. Related Articles | Metrics

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The correlation analysis between PfKelch13 gene mutation of Plasmodium falciparum isolates and artemisinin resistance in Yunnan Province SUN Ai-ming,WANG Jian, DONG Ying, CHEN Meng-ni,XU Yan-chun, DENG Yan, MAO Xiang-hua 2018, 35 (1):  21.  Abstract ( 351 )   PDF   Save In this paper, we analyed the correlation between PfKelch13 gene mutation and artemisinin resistance and the differentiation of PfKelch13 gene among populationsin in Myanmar. The results showed that in 190 DNA sequences of kelech domain in PfKelch13 gene from Yunnan blood samples had 13 haplotypes, the expected heterozygosity (He) was 0.0449 and the nonsynonymous mutations sequences accounted for 34.7%(66/190), the F446I mutation rate was the highest with 25.3%(48/190); 19 haplotypes were obtained from 194 DNA sequences from Myanmar bloods samples, the expected heterozygosity (He) was 0.0449 and the nonsynonymous mutations sequences accounted for 58.8% (114/194), and F446I mutation rate was the highest with 43.3%(84/194); the genetic diversity of PfKelch13 gene in kelch domain of Plasmodium falciparum population in Yunnan Province was relatively high, the index of genetic differentiation of two populations was 0.0410 (Fst＝0.0410, P＜0.05), and the variation within the population and amomg the populations accounted for 95.90% and 4.10% respectively. The expected heterozygosity (He) of the OR value between artemisinin resistance and haplotypes was 0.0449 and the nonsynonymous mutations sequences accounted PfKelch13 gene kelch domain at 446 site mutation was 1.640 (95%CI：1.284~2.095) and the OR value of the 12 site mutations was 1.840 (95%CI：1.412-2.398). Therefore, the differentiation degree of Plasmodium falciparum in Yunnan and Myanmar was low in the PfKelch13 gene kelch domain, so the genetic association between the PfKelch13 gene mutation and artemisinin resistance in Myanmar is suitable for the report of Plasmodium falciparum population in Yunnan. Related Articles | Metrics

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Comparison of expression and activity of anti-AFB1 scFv in Escherichia Coli and Pichia Pastoris HU Li, WANG Xiao-hong 2018, 35 (1):  26.  Abstract ( 314 )   PDF   Save In order to improve the expression yield and biological activity of aflatoxin B1 (AFB1) single chain Fv fragment (scFv), scFv genes from hybridoma cell lines 2C10 by reverse transcription method and gene splicing technology were constructed into the expression vectors pET-30a and pPICZαA respectively to compare the results. Then the constructed expression vector pET-30a-pelB-scFv was transformed into BL21(DE3) to express protein induced by IPTG. The constructed expression vector pPICZαA-scFv was linearised and transformed into Pichia pastoris by electroporation, and the transformants were induced by methanol，and the anti-AFB1 scFv was expressed. The expression proteins were purified and determined by SDS-PAGE, western blot and ELISA.The results of SDS-PAGE showed that anti-AFB1 scFvs were highly level expressed successfully, with the expression yield of about 34 mg/L in E.coli expression system and 132 mg/L in Pichia Pastoris expression system, and the goal protein is about 29 kua. The results of western blot and ELISA demonstrated that the recombinant scFv could combine with AFB1 specifically. and the sensitivity of anti-AFB1 scFv was 40 μg/mL and 35 μg/mL respectwely. Compared with anti-AFB1 scFv expressed in E.coli BL21(DE3)， the sensitivity of scFv expressed in Pichia pastoris was a little better， but there was still some room for improvement． Related Articles | Metrics

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The correlation analysis of soil enzyme activity and AM fungi in the rhizosphere soil of different year-old Paeonia ostii LI Zhi-ya, YANG An-na, TANG Dong-mei, WANG Jian-zhong, YANG Fan 2018, 35 (1):  32.  Abstract ( 255 )   PDF   Save The method of alkaline hydrolys acid fuchsin staining was used for determining the colonization of AM fungi in the root of Paeonia ostii. The content of soil hypha, spore density in soil and the activity of soil enzyme were also determined. The results showed that all roots samples were infected by AM fungi and formed the structure of Arum-type AM. AM fungal colonization rate in Triennial Paeonia ostii is smaller than the four-year-old Paeonia ostii′s which was smaller than the Biennial Paeonia ostii′s. Hypha colonization rate, content of paeonol and spore density in different years′ root samples showed the same trend as the AM fungal colonization rate. The colonization of vesicle in Triennial Paeonia ostii was smaller than that in Biennial Paeonia ostii′s, the biggest one was four-year-old Paeonia ostii′s. The colonization of hyphal coil showed the same trend as the colonization of vesicle.The activity of acid phosphatase, urease, sucrase and polyphenol oxidase was positively correlated with the content of soil hypha and negatively correlated with the colonization of Vesicle. The activity of acid phosphatase, sucrase and polyphenol oxidase was positively correlated with the colonization of hyphae circle and spore density, but negatively correlated with the urease activity. The activity of acid phosphatase was positively correlated with the colonization of hypha and AM fungi, but negatively correlated with the urease, sucrase and polyphenol oxidase′s activity. The research reveals the symbiotic relationship of AM fungi with the different year-old root of Paeonia ostii during period of dormancy and determinates the content of cortex moutan phenol and the activity of soil enzyme. Related Articles | Metrics

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Effects of phycosphere Bacillus sp. WPySW2 on physiology of Pyropia haitanensis XIONG Yu-qin, YANG Rui, YANG Hua-tian, SUN Xiao-xiao 2018, 35 (1):  37.  Abstract ( 296 )   PDF   Save Pyropia haitanensis collected from the culture area of the Xiangshan, Zhejiang Province, was pure cultured and co-cultured with Bacillus sp. WPySW2 from Porphyra-epiphytic bacteria under different time (0, 6, 12, 24, 48 and 72 h). They grew at the normal culture temperature (20℃), and the physiology were measured, including the activities of (POD), superoxide dismutase (SOD), and also photosynthetic pigments, total protein, free proline and malondialdehyde (MDA) levels. The results showed that the content of photosynthetic pigments and protein in co-culture group was better than that in the control. Both were no significant difference in antioxidant enzyme activity because of cell membranes been not oxidized (P>0.05). The two groups produced excessive membrane lipid peroxidation and osmotic pressure regulating substance, but the control group produced more (P<0.05). It was speculated that the phycosphere epiphytic bacteria would provide nutrients for the algae in phycosphere to affect their growth status and the accumulation of nutrients. Related Articles | Metrics

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Screening of endophytes in Zizyphus jujube and the analysis of their secondary metabolites DENG Zhen-shan, WEI Ting-ting, GAO Fei, LIU Yu-zhen, SU Rui, CHEN Bang-kai, MO Da-rui 2018, 35 (1):  42.  Abstract ( 280 )   PDF   Save To fully exploit and utilize the resources of wild jujube, look for endophytes producing active substances such as flavonoids, provide germplasm resources for new drugs, isolate and purify the endophytes of the jujube stem by tissue separation method and sutural separation method, confrontation method for antibacterial activity was measured. The strains with strong antibacterial activity were B-08-12 and B-08-20, the diameter of the zone of inhibition was (31.00±0.78) mm, and the strains were fermented, extracted with organic reagents, investigated by thin layer chromatography (TLC) and also analyzed by UV spectroscopy Photometer for further detection. TLC detection showed that the fermentation product of strain B-08-20 had the same color speckles at the Rf of 0.43 as the Rf value of the jujube stem extract, and the active compounds were preliminarily identified as flavonoids or its analogues. Related Articles | Metrics

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Molecular identification of fairy shrimp from Qiubei County, Yunnan Province LI Rong, CHEN Yan-yan, HE Zhong-ting, YANG Hong-fu, LI Chun-qing,ZENG Ben-juan, CHEN Shan-yuan, XIAO Heng 2018, 35 (1):  46.  Abstract ( 319 )   PDF   Save In order to clarify the taxonomic status of fairy shrimps from Qiubei County of Yunnan Province at the molecular level, this study applied PCR amplification and sequencing methods to determine the COI gene (658 bp) and 18S rDNA gene (325 bp) sequences of 30 fairy shrimp samples. The BLAST similarity analyses based on NCBI database showed that COI gene and 18S rDNA gene sequences were highly similar to those of Streptocephalus sirindhornae, with identity values of 96% and 100%, respectively. Sequence analyses revealed that there were 15 parsimony informative sites and 22 variable sites that defined 8 haplotypes in the COI gene sequences and there was one parsimony informative site and one variable site that defined two haplotypes in 18S rDNA gene sequences. In addition, the genetic distances and phylogenetic relationships were analyzed based on the combined dataset including our sequence data and homologous COI sequences of some Anostraca families in GenBank. The results showed that: interspecific average genetic distance between fairy shrimps from Qiubei and Streptocephalus sirindhornae of family Streptocephalidae was 4.3%, being at intraspecific level; while interspecific average genetic distance between fairy shrimps from Qiubei and Thamnocephalus platyurus of family Thamnocephalidae was 21.1%, being at interspecific level. In addition, phylogenetic trees showed that fairy shrimps from Qiubei and Streptocephalus sirindhornae clustered together as a monophyletic clade, with support values of 100% and 72% on COI and 18S rDNA trees, respectively. These results mentioned-above indicated that the fairy shrimp species from Qiubei County of Yunnan Province was Streptocephalus sirindhornae of family Streptocephalidae,but was not species of family Thamnocephalidae. At the same time, it was suggested that COI gene is a more suitable marker than 18S rDNA gene for molecular identification of fairy shrimps. Related Articles | Metrics

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Research on the differences between different geographic populations of tree shrew (Tupaia belangeri ) Mc1r gene#br# FU Jia-hao, HE Yi-fan, ZHU Wan-long, GAO Wen-rong, WANG Zheng-kun 2018, 35 (1):  52.  Abstract ( 229 )   PDF   Save Melanocortin receptor 1 gene (Melanocortin Receptor 1, Mc1r) is the major candidate gene in the regulation of mammalian coat color, which plays an important role in animal′s coat color. There were 112 Tupaia belangeri collected from 10 geographical populations， and 954 bp mc1r sequence was amplified by PCR and analyzed. The results showed that the 954 bp mc1r sequence encoded 317 amino acids. The secondary structure of protein was mainly composed of α-helix containing 7 transmembrane domains. Phylogenetic tree of mc1r showed that 10 populations of tree shrews were clustered into 4 branches:CladeⅠ(HaiNan Population), CladeⅡ(DaXin Population)， CladeⅢ(PianMa Population) and CladeⅣ(Dali, Mengla, Hekou, Kunming, Leye, Xingyi and Xichang populations) .The results showed that different living environments cause the differences in tree shrews’s coat color. Related Articles | Metrics

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Study on the multiple paternity in the Yangtze vole using microsatellite markers GUO Zong-ming, WANG Yong, GUO Cong 2018, 35 (1):  56.  Abstract ( 249 )   PDF   Save A method of microsatellite marker was employed to confirm multiple paternity in the Yangtze vole(Microtus fortis calamorum) distributed in Dongting Lake. Six pairs of primers were screened for better PCR products. After PCR, gel electrophoresis and silver staining, the alleles of seven mothers and their offspring were analyzed. The results showed that two litters were sired by more than two male voles, respectively. Mating behavior of the vole was characterized with multiple paternity.  Related Articles | Metrics

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Flow cytometric analysis of fat cells in Tupaia belangeri under cold acclimation ZHU Wan-long, HOU Dong-min, SUN Shu-ran, WANG Zheng-kun 2018, 35 (1):  60.  Abstract ( 242 )   PDF   Save In the present study, it was first successful using of adipose tissue making method of flow in Tupaia belangeri, and the flow cytometry was also firstly used to compare and analyze fat cells under cold acclimation. The results showed that 5.70% of white adipose tissue (WAT) in abdominal omental and groin had single R1 group and showed positive UCP1 on 28 d of cold acclimation. New cell population R2 group appeared in WAT in the 49th day, including 69.64% the cells in R1 group of showing positive for UCP1. 95.28% of the cells in R2 had showing positive for UCP1. Cold acclimation makes UCP1 expression positive cell population in WAT. All of the results suggested that cold acclimation not only made the brown adipose tissue hyperplasia, the production of thermal activity increased, but also induced the beige fat cells appeared in WAT. Related Articles | Metrics

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The method for dissection and immunofluorescence staining of the adult Drosophila brain WANG Gua-xiu 2018, 35 (1):  64.  Abstract ( 649 )   PDF   Save Drosophila, as a model organism, has made a valuable contribution to the research in neuroscience, and because of its powerful gene, it has been widely used as models of neurodegenerative diseases. The Drosophila brain has become increasingly useful for studies of neuronal wiring and morphogenesis and the model of learning and memory. Here， we described a basic method for dissection and immunofluorescence staining of the adult Drosophila whole brain. A part of the adult drosophila brain structure can be seen after dissection under confocal microscopy, which provides an ideal drosophila brain anatomical method. Such high-resolution anatomical study of the brain is also important in characterizing the organization of neural circuits using genetic tools such as GAL4 enhancer trap lines, as Drosophila has been intensively used for studying the neural basis of behavior. Related Articles | Metrics

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Avain community diversity and seasonal dynamic in Jiaogang Lake National Wetland Park PING Lei,ZHOU Li-zhi 2018, 35 (1):  68.  Abstract ( 309 )   PDF   Save From October 2014 to September 2015, we surveyed birds using the line transect and point-count methods in Jiaogang Lake National Wetland Park in Huainan, Anhui Province, and the avain diversity and seasonal dynamics were analyzed and reasearched. A total of 96 bird species were recorded, belonging to 15 orders and 37 families, of which 41 (42.7%) were resident birds, 25(26.0%) winter migrant species, 21 (21.9%) summer migratory birds and 9(9.4%) transient birds. Avifauna mainly consisted of Palaearctic species and Oriental species,but widely distributed species were also observed. The Shannon-Wiener index was the highest(1.99) in preserve area but the lowest(1.27) in sightseeing area， and annual diversity is summer>spring>autumn>winter. And the similarity coefficient of bird communities was the lowest between preserve area and sightseeing area(0.427), but the highest between preserve area and sightseeing area(0.726). Moreover, some suggestions for the following construction and management style were provided. Related Articles | Metrics

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Two new records of Cerambycidae in Anhui, China XU Qin, LI Wen-bo, LIU Nai-yi, YIN Ruo-chun, FANG Jie 2018, 35 (1):  73.  Abstract ( 269 )   PDF   Save Two species of Oberea diversipes and Oberea fuscipennis belonging to Cerambycidae were newly found in Yaoluoping National Nature Reserve，Anhui Province in August 2015. There morphological characteristics and distribution were further described in detail. Related Articles | Metrics

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Community structure characteristics of phytoplankton in Digou coal mining subsidence lake WAN Yang,ZHOU Zhong-ze,WANG Chen-chen,LIN Zhi,WU Qi-li 2018, 35 (1):  75.  Abstract ( 247 )   PDF   Save In order to study space-time variation of phytoplankton and its relationship with water environmental factors in Digou coal mining subsidence lake, the phytoplankton community was investigated from August 2015 to June 2016. Results showed that a total of 155 species of phytoplankton were identified, which belong to 8 phyla, 10 classes, 17 orders, 32 families and 62 genera. The species number of Chlorophyta, Bacillariophyta and Cyanophyta accounted for more than 75% of phytoplankton species. During the investigation, the main dominant species were Phormidium tenu, Oscillatoria acutissima, Synedra ulna, Chroomonas acuta, etc. The variation range of the density and biomass of phytoplankton respectively was 22.68×105-290.24×105cells/L and 2.47-7.41 mg/L. The density of phytoplankton changed significantly (F=15.48, P<0.05) , and the overall trend was to rise after the first drop. Biomass exhibited a similar temporal behavior to that seen in the density. The density and biomass of Cyanobacteria were dominant in most of the time. The spatial differences of Shannon-Wiener index (H′) were obvious, and water system communication improved phytoplankton diversity in subsidence lake. Redundancy analysis (RDA) indicated that water temperature, transparency, total phosphorous and nitrate nitrogen were important factors affecting the variations of phytoplankton community in the subsidence lake, and water temperature was the key factor affecting the temporal variation of phytoplankton. Digou mining subsidence lake was eutrophic, and the eutrophic characteristion of phytoplankton community was obvious. Meanwhile, the lake had a potential risk of blue algae bloom. Related Articles | Metrics

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The vessel elements and perforation plates of secondary xylem LI Hong-fang 2018, 35 (1):  82.  Abstract ( 321 )   PDF   Save The vessel elements in the secondary xylem of two species from Proteaceae were observed by scanning electron microscope(SEM), and the difference in morphology of vessel and pits distributed of lateral wall were shown for the first time. The perforation plates of two species are simple with tail or without tail, all pits distributed in lateral wall of vessel elements in Grevillea banksii var. forsteri are alternate, while some of Banksia integrifolia are opposite-alternate, the morphology of vessel in Banksia integrifolia are primitive than Grevillea banksii var. forsteri. Related Articles | Metrics

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The structure, function and generation mechanism of clusterin DONG Qiao-mei, ZHAO Li, TENG Yong-jun 2018, 35 (1):  85.  Abstract ( 1417 )   PDF   Save Clusterin (CLU) is a multifunctional protein existing in distinct subcellular components. It was first reported in 1983 as an adhesion glycoprotein in goat semen by Blaschuk, then was found involved in numerous physiological processes and associated with many pathological states (neurodegenerative diseases, immune diseases, aging, cancer, etc.). CLU plays different roles, and even in opposing aspect, which confused researchers. To summarize results of different research groups, we will have a more clear understanding of this protein. Currently, it is considered that clusterin may encode different protein products (primarily secreted CLU (sCLU) and karyotype CLU (nCLU)), with different cellular localization and play different biological functions. In this paper, the structure, function, generating mechanism and the signal network of this protein will be reviewed. Related Articles | Metrics

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CRISPR-Cas system and its research progress in mycobacterium tuberculosis ZHAI Xiao-qian,BAO Lang 2018, 35 (1):  89.  Abstract ( 418 )   PDF   Save CRISPR-Cas (the clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) system acts as an adaptive immune system protects prokaryotes from viruses and plasmids that represented in mycobacterium tuberculosis. And there is evidence that CRISPR-Cas system modulates other processes-the genetic regulation of virulence and genome evolution. At the same time, modified CRISPR-Cas system constitutes a powerful tool for genetic engineering. CRISPR-Cas system shows unprecedented complexity and diversity. In this review, introduction the mechanism, application of genome engineering and recent progress in cas genes of type III-A CRISPR-Cas system are summarized for providing a theoretical basis for the study of mycobacterium tuberculosis. Related Articles | Metrics

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Research progress in drug addiction memory and its neural circuitry LI Ping, WANG Xiao-qin, SUN Zhen-wu, WANG Gong-wu 2018, 35 (1):  93.  Abstract ( 432 )   PDF   Save Drug addiction has produced serious impairment on human health and threatens the security and stability of the whole society. Therefore, how to prevent and cure drug addiction and to uncover the working mechanisms are always the hot spots of research at home and abroad. In recent years, the brain neural network of human and animal has been clarified in detail, especially underlying the rapid application of the optogenetics technology. Scientists have made some important progress in drug addiction related brain regions and circuits. The drug addiction, especially the psychological addiction, has been regarded as a brain disease, a pathological, solid memory, or mnemonic disease, which we call addiction memory. Therefore, it would be an efficient strategy for treatment of craving and relapse by which to disturb the addiction memory processes according to memory mechanism. Addiction memory is involved by many brain areas (such as the hippocampus, the prefrontal cortex, the ventral tagmental area, the rostromedial tegmental nucleus, and the habenula), which are interconnected by circuits and play crucial roles in normal learning and memory. This paper mainly reviews the recent research progress in treatment of addiction memory and the underlying neural circuits. Related Articles | Metrics

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The research progress of curcumin antitumor mechanism CHANG Na, WANG Qiao-xia, WANG Xiao-ping, ZHAO Qian 2018, 35 (1):  97.  Abstract ( 396 )   PDF   Save To look for a kind of natural medicines which characterized of safety, low toxicity and efficient to improve patient′s life quality has been a hotspot of research on domestic and foreign scholars. Curcumin is a monomer which extracted from traditional Chinese medicine, such as turmeric, rhizoma zedoariae, radix curcumae. A number of studies have confirmed that curcumin has the function of anti-tumor, anti-inflammatory, antioxidant and other biological activities. Curcumin has a good development prospect. The advantages of curcumin are large ranges of anticancer; a wide range of pharmacological effects; little side-effects and so on. This article summarizes the anti-tumor mechanism of curcumin and hope to provide references for the clinical application.  Related Articles | Metrics

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Diagnosis and monitoring of fatty liver in dairy cows HAO Ning, ZOU Su-ping, TAO Huan-qing, MING Peng-fei, XU Yi-zhong,NIE Xing-can, FENG Shi-bin, WANG Xi-chun, WU Jin-jie, LI Yu 2018, 35 (1):  101.  Abstract ( 349 )   PDF   Save Dairy cow fatty liver is an important and common mass disease in the process of cow feeding， which is mainly related to the metabolic balance of energy. Because of its incidence generally accompanied by body conditions, reproductive capacity and decline in milk production, large-scale dairy cattle feeding early on the diagnosis and detection of fatty liver dairy is a cost-saving necessary. This paper summarizes the causes of fatty liver from dairy cows, methods of diagnose and monitor, and the internal relationship between fatty liver in dairy cows, inorder to make a more theoretical basis for the study of fatty liver. Related Articles | Metrics

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Screening of low-yield citrinin monascus by compound mutation breeding ZHUANG Xiao-xiao,WANG Yu,LI Kuo-kuo,SHEN Xiao-rui 2018, 35 (1):  104.  Abstract ( 264 )   PDF   Save  In order to screen out low citrinin yield strain, as a starting strain, the high-yield lovastatin Monascus strain M50-2 was treated by ultraviolet, which was selected by N+ ion beam mutagenesis. The starting strain was irradiated at 30 cm under a 30 W UV lamp, and the dose was 30, 60, 90, 120 and 150 s. The contents of citrinin and lovastatin in the fermentation products were determined by high performance liquid chromatography (HPLC) with fluorescence detection (λex=331 nm, λem=500 nm) and ultraviolet detection (λ=238 nm) after the liquid fermentation of the screened mutagenized strain. The results showed that an excellent strain M120-1 was obtained by ultraviolet mutagenesis. The best strain M120-1 yielded 2.15 mg/L citrinin, which was 96.6% lower than the original strain. However, lovastatin production was 0.338 mg/mL, which was only decreased by 16.5%. To examine the genetic stability of M120-1, the strain was continuously sub-cultured to the 12th generation, showing that the strain had good genetic stability. Related Articles | Metrics

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Soluble expression recombinant human interleukin-2 in Escherichia coli LI Guan-ying, LI Hai-hong, XU Shi-xun, PAN Xiao-yu 2018, 35 (1):  107.  Abstract ( 272 )   PDF   Save This work aims to explore the soluble expression and biological activity of interleukin-2; recombinant fusion protein MBP-rhIL2 was successfully expressed into soluble form in E.coli BL21 (DE3). The purification of MBP-rhIL2 was conducted through amylose resin. The rhIL-2 was efficiently released by the cleavage of protease Factor Xa from the fusion protein. Bioactivity analysis showed the biological activity of purified rhIL-2 is 4.4 ×106 IU/mg. Related Articles | Metrics

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A bird and butterfly-attractive ecological landscape  with Woodfordia fruticosa LIN Shi-shi, WANG Xiao-ming, ZHANG Shou-zhou, WANG Ying-yong, LONG Dan-dan, SUN Yan-jun 2018, 35 (1):  111.  Abstract ( 310 )   PDF   Save In South China, a bird-attractive and butterfly-attractive ecological landscape can be created by shrub Woodfordia fruticosa in 3-5 years, the Pycnonotus jocosus, Pycnonotus sinensis, Aethopyga christinae, Zosterops japonicas, Dicaeum cruentatum, Dicaeum ignipectus, Chloropsis hardwickii and Phoenicurus auroreus will be attracted. And the butterfly such as Papilio paris, Papilio helenus, Papilio polytes, Graphium sarpedon and Delias pasithoe will be attracted, too. Suggestions for Woodfordia fruticosa planting was proposed to the whole ecological landscape creation. Related Articles | Metrics

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The application of Adobe Illustrator in drawing plant structure#br# MA San-mei， LIU Chao-yu, WANG Yong-fei 2018, 35 (1):  115.  Abstract ( 290 )   PDF   Save In the experiment of plant biology, students are usually asked for draing on the paper with pencil. The painting often is black and white. In order to draw colorful painting quickly, Adobe Illustrator is used to draw the plant structure. Colorful pictures of plant structure are more attractive than that are black and white, which can make it easy for students to understand and recognize plant structure. Related Articles | Metrics

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Exploration and practice of module teaching reform for molecular biology DUAN Rui-jun, YANG Xi, LIANG Jian, LI Chang-zhong 2018, 35 (1):  118.  Abstract ( 289 )   PDF   Save Molecular biology has become the most basic, important and frontier main course in life science, but its teaching mode still uses the traditional teaching way and is lack of innovation. This article introduces the status of the module teaching system in molecular biology and its application. The constructed ideas and reformation contents are elucidated. The course content is divided into four modules，these are " genetic information transmission, molecular biological technology, gene expression and regulation, application and development of molecular biology ". The corresponding experimental teaching is divided into "basic, comprehensive and design, innovative " experiment module. The practicing experiences and preliminary effects in the implementation process are summarized and the future of module teaching reform are also discussed as well. Related Articles | Metrics

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Reform and innovation of experimental teaching of cell biology#br# HUANG Xuan, FU Ai-gen, ZHAO Yu-wei, LI Jun-lin， YAN Xing-rong 2018, 35 (1):  121.  Abstract ( 269 )   PDF   Save Cell biology is an important basic course in undergraduate biology related course, its relevant experimental teaching content of the course has a decisive role in training high-quality comprehensive talents. In this paper, aiming at the shortage of cell biology, we improve the traditional experimental teaching, increase experimental teaching contents, introduce innovative experiment, change the teaching mode, strengthen the teaching team building, enhance the teaching conditions and other aspects of the reforms of experimental teaching, to cultivate students' innovation consciousness and basic scientific literacy. The methods and measures of undergraduate′s experimental teaching of cell biology have been improved in practice. Related Articles | Metrics

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Course design and teaching understanding of glycolysis in biochemistry SHI Mei, LI En, LIU Jun-hong, LIU Hui-jun, ZHANG Ting-ting 2018, 35 (1):  125.  Abstract ( 262 )   PDF   Save Biochemistry is one of the most important basic courses in life sciences, and glycolysis is the first metabolic pathway of dynamic biochemistry. The glycolysis is a good way to understand glycometabolism and even the whole material metabolism. Therefore, this article introduces the course design and teaching experience of "glycolysis" in detail, such as introduction of new lesson, explanation of content, imparting knowledge and educating people. It aimed to provide some new thinking for teaching of biochemistry. Related Articles | Metrics

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Practice of flipped classroom integrated with TBL teaching methodology in teaching medical biology experiments YANG Xiao-yan, LI Gui-qin, ZHAO Qian, YIN Jie, LEI Xiao-yong 2018, 35 (1):  127.  Abstract ( 221 )   PDF   Save tTo explore the influence of the flipped classroom combined with TBL (team based learning) teaching mode on the teaching of medical biology experiments, 64 preventive medicine students were selected and randomly divided into 2 groups: control group (n=32) with traditional teaching method and experimental group (n=32) with flipped classroom integrated with TBL teaching methodology. Effects of teaching were evaluated by score evaluation and questionnaire survey. The difference between average scores of comprehensive ability evaluation in the experimental group (85.4±9.3) and the control group (70.3±11.5) is remarkable significant. The results of survey showed that more than 90% of experimental group students thought flipped classroom combined with TBL teaching mode could help to improve learning interest, independent thinking and operation ability and team cooperation. Flipped classroom mode, which combines with the TBL teaching methodology could not only effectively improve the students′ ability of autonomous learning, but also significantly promote medical biology experiment teaching effect. Related Articles | Metrics