Abstract: The course of “fundamentals of plant cloning technology and applications” is a general course offered by college of life science of Zhejiang University for all undergraduates. The course covers culture-medium preparation, aseptic manipulation, induction of callus and embryoid, acclimation of tissue culture seedlings, which embodies the cross-integration of biology, forestry and horticulture. This paper explores the cross-fusion knowledge points between the curriculum and other professional fields from the aspects of curriculum teaching objectives, curriculum system construction and curriculum assessment methods. Based on the problem-oriented teaching methods and the combination of online and offline teaching methods, the role of curriculum teaching content and teaching methods in cultivating interdisciplinary students is explored. The results of three years’ teaching practice show that the students’ academic performance, interest in scientific research and experiment, and curriculum construction level have all been improved. The interdisciplinary teaching design has enhanced students’ serial cognition of interdisciplinary knowledge, expanded students’ horizons, and laid a good foundation for the cultivation of innovative talents with multi-disciplinary background.

Key words: interdiscipline; fundamentals of plant cloning technology and applications, curricula construction, teaching reform, teaching method