Table of Content

18 October 2022, Volume 39 Issue 5 Previous Issue   

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Functional comparison of phenazine synthetic proteins PhzA and PhzB KONG Deyu, WANG Zheng, NIE Chenxi, ZHANG Xuehong 2022, 39 (5):  1.  doi: 10.3969/j.issn.2095-1736.2022.05.001 Abstract ( 508 )   PDF   Save In order to explore the functional differences between PhzA/B and the effects on the synthesis of PDC and PCA, after comparing, screening and synthesizing the phzA and phzB genes from different bacteria were studied. The result showed that PDC and PCA could be detected in seven mutants, in which heterologous phzB genes replaced the endogenous phzA and phzB gene at the same time. However, PDC could not be detected in the secondary metabolites of five mutants, in which two heterologous phzA genes replaced the endogenous phzA gene and three heterologous phzB genes replaced the endogenous phzB gene, and the simultaneous presence of phzAB wound promote the synthesis of PCA. Moreover, structural analysis showed that there were some differences in some regions of PhzB proteins from different bacteria. The protein structures of PhzA and PhzB were similar, but PhzA lacked the corresponding active amino acid sites of PhzB. In conclusion, PhzA/B proteins from different bacteria had relatively high sequence similarity, but had certain differences in structure and function. They all played an important role in the synthesis of phenazine compounds. Among them, the function of PhzB protein was important to catalyze the synthesis of PDC, while PhzA protein could promote the synthesis of PCA. Related Articles | Metrics

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Effect of meiotic cohesins RAD21L and REC8 on DNA double strand break repair Wuyundalai, Meirong 2022, 39 (5):  8.  doi: 10.3969/j.issn.2095-1736.2022.05.008 Abstract ( 353 )   PDF   Save In order to reveal the molecular mechanisms in the process of homologous chromosome recombination, spermatocytes ofmouse were used to observe the localization relationship between RAD21L and REC8 and the recombination intermediates RAD51 and MSH4 by high-resolution microscope (3D-SIM), and to compare the correlation of the recombination intermediates between RAD21L and REC8. The results showed that RAD21L was more likely to express in the vicinity of recombinant intermediates than that of REC8, and even express insome parts of homologous chromosomes that had not yet been linked. Interestingly, these parts were just where recombinant intermediates existed. It is believed that RAD21L acted indirectly on the recombination of homologous chromosomes. When DSBs occurred on the chromosome, RAD21L would accumulate and fix around DSBs to ensure the successful repair of DSBs, while the repair of DSBs was a secondary function for REC8. Related Articles | Metrics

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Exploration of potential diagnostic markers for COVID-19 based on peripheral blood gene expression profile ZHANG Sijia, ZHANG Shun, CAI Ting 2022, 39 (5):  13.  doi: 10.3969/j.issn.2095-1736.2022.05.013 Abstract ( 260 )   PDF   Save The dataset GSE161731 was downloaded from the Gene Expression Omnibus(GEO) database. Variation analysis and Lasso regression were used to search for the key genes which can distinguish early COVID-19 patients. Diagnostic efficacy was evaluated by ROC curve. Biomarkers of different disease groups were confirmed by co-expression network constructed (WGCNA). KEGG enrichment analysis was applied to explain the function of gene modules and related signal pathways. A total of 509 differential genes were screened, and 17 potential diagnostic markers for early-stage COVID-19 patients were identified including MYBL2, PKMYT1, HJURP, TCN2, TTC24, ESPL1, GZMK, RPA3, ATP5F1E, CD2, ZAP70, IL15RA, NFYB, CIAO2A, PLRG1, GPATCH11 and MRPL33. KEGG results showed that these differentially expressed genes were mainly related to cell cycle, T cell immunity, and oxidative phosphorylation. The COVID-19 specific gene modules identified by WGCNA analysis were mainly involved in physiological activities such as protein synthesis and ribosomes oxidative phosphorylation. Related Articles | Metrics

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Enterovirus 71 induces has-miR-200b expression to promote viral replication HU Jingping, WANG Yan, FU Yuxuan 2022, 39 (5):  21.  doi: 10.3969/j.issn.2095-1736.2022.05.021 Abstract ( 174 )   PDF   Save The role of miR-200b in EV71 replication was investigated by high-throughput sequencing, luciferase reporter gene assay and Western Blot. The results showed that the expression of has-miR-200b increased significantly in host cells after EV71 infection by high-throughput miRNA sequencing, and specific inhibition of miR-200b expression could inhibit EV71 replication. Luciferase reporter gene assay showed that miR-200b could directly target the 3′-UTR region of IFIT5. Further study confirmed that the increased expression of miR-200b induced by EV71 infection could inhibit IFIT5 expression and thus supress the type I interferon response. It is shown that EV71 infection can induce the increase of miR-200b expression in host cells and then inhibit the expression of IFIT5, resulting in the inhibition of intracellular interferon response, which facilitates EV71 replication. Related Articles | Metrics

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Role of Schizosaccharomyces pombe SpHMT1 gene in cadmium tolerance and accumulation in Arabidopsis thaliana HUANG Jing, ZHOU Hao, LI Meilin, FU Qiang 2022, 39 (5):  26.  doi: 10.3969/j.issn.2095-1736.2022.05.026 Abstract ( 162 )   PDF   Save To study the function of Schizosaccharomyces pombe heavy metal tolerance factor 1 (SpHMT1) gene in Cd tolerance in Arabidopsis thaliana, the SpHMT1 gene encoding the PCs-Cd transporter in Arabidopsis was overexpressed. It was found that SpHMT1 enhanced the tolerance and accumulation of Cd, Cu, As and Zn, and failed to confer Cd2+ tolerance and accumulation when GSH and PC synthesis was blocked by L-buthioninesulfoximine (BSO). SpHMT1 was localized in tonoplast in Arabidopsis, and the energy-dispersive X-ray analysis showed that S was more present in vacuoles in transgenic plants, suggesting that the function of SpHMT1 in Arabidopsis depended on phytochelatins. On the other hand, constitutive expression of SpHMT1 delayed root to shoot transport of the metals, suggesting that roots can serve as a sink, with further reduction of metal content in shoots. Related Articles | Metrics

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GhVTC2 gene promotes elongation of tobacco BY2 suspension cells and Arabidopsis main roots DONG Xiangyu, CHEN Lihua, TU Zehang, CAO Aiping, WANG Fei, LI Hongbin 2022, 39 (5):  32.  doi: 10.3969/j.issn.2095-1736.2022.05.032 Abstract ( 347 )   PDF   Save In order to study the function of VTC2 gene in cotton fiber development and cell elongation, a GhVTC2 gene was identified from fast elongating fibers of cotton. Expression analysis showed that GhVTC2 accumulated significantly during the rapid elongation period of cotton fibers, suggesting its important role in cell growth and development. The plant overexpression vector 35S:GhVTC2 was constructed and transformed into tobacco BY2 suspension cells and Arabidopsis thaliana; compared with that of the non-transformed Arabidopsis plants and tobacco cells, the cell length was increased significantly in transgenic tobacco cells, and the elongation of main roots in transgenic Arabidopsis was significantly promoted. The contents of AsA in transgenic tobacco cells and Arabidopsis root were increased. The promoter of GhVTC2 gene was obtained by cloning, and sequence analysis showed that the promoter contained multiple ethylene responsive elements ERE. The 5′ deletion vectors of different promoter sequences with or without the ERE element were constructed and transformed into tobacco leaves treated by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC). The results showed that ACC significantly promoted the activity of ERE-containing promoters. An effective reference is provided for studying the function and regulatory mechanism of GhVTC2 gene in cell elongation and development. Related Articles | Metrics

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Differential analysis of gene expression profile of Serratia sp. producing red pigment LI Jingyu, ZHANG Xiaochong, LI Yannan, YUAN Cunxia, LIU Jianli, JIN Duo, MA Zhishan 2022, 39 (5):  38.  doi: 10.3969/j.issn.2095-1736.2022.05.038 Abstract ( 192 )   PDF   Save Transcriptome sequencing was used to deeply understand the gene expression profile of a strain of Serratia producing red pigment at different growth stages (pigment and non-pigment producing) which was isolated and purified in the laboratory. A total of 2512 differentially expressed genes were obtained from the gene expression profiles of different growth stages, of which 1317 were significantly up-regulated. Pathway enrichment analysis showed that there was a significant enrichment trend in quorum sensing function. In the pigment production stage, the AI-2 kinase lsrk was highly expressed, and the LuxR family transcription regulator sdiA was also highly expressed. And 1195 genes were significantly down regulated, and pathway enrichment analysis showed that there were significant enrichment trends in ribosome, flagellum assembly, TCA cycle, peptidoglycan biosynthesis, aminoacyl tRNA biosynthesis, prokaryotic carbon fixation, cationic antimicrobial peptide resistance, bacterial chemotaxis, oxidative phosphorylation, alanine, aspartate and glutamate metabolism, and bacterial secretion system. Related Articles | Metrics

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Inhibitory effect and molecular mechanism of medicinal plants formula MZL-1 against MRSA LI Dong, XIANG Kun, CHANG Miao, ZHONG Jialing, HE Jiameng, MAO Canquan 2022, 39 (5):  45.  doi: 10.3969/j.issn.2095-1736.2022.05.045 Abstract ( 194 )   PDF   Save Methicillin-resistant Staphylococcus aureus (MRSA) was used to study the anti-MRSA effect and preliminary molecular mechanism of the medicinal plant formula MZL-1 developed in the laboratory. The antibacterial effect of MZL-1 was confirmed by the double dilution method, and the relative conductivity, alkaline phosphatase activity change and scanning electron microscope observation were detected to analyze the effect of MZL-1 treatment on the destruction and integrity of the cell wall and membrane of MRSA. Flow cytometry was utilized to determine the effect of MZL-1 on MRSA for cell apoptosis. Network pharmacology was applied to explore the possible molecular mechanism of the anti-microbial effects of MZL-1. The result showed that the MIC of MZL-1 to MRSA was 7.5%. The increase in the activities of relative electric conductivity and alkaline phosphatase as well as the significant changes in morphology and damages observed by scanning electron microscopy indicated that MZL-1 obviously disrupted the integrity of MRSA cells and hence caused the overflow of the cell contents. Flow cytometry analysis found that a large number of cell apoptosis occurred in the early stage. Network pharmacology discovered 29 compounds and 23 target genes for MZL-1 in anti-microbial infection, and RELA may be the core gene. Gene Ontology(GO) enrichment analysis showed that target genes were mainly involved in the cell's response to small molecules, cytokines and transcription factors. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis found that the pathways involved mainly focused on inflammation, apoptosis, immune function, and bacterial and viral infections. Based on the current research, the formula MZL-1 might be potentially developed to be a plant-derived drug against MRSA and other drug-resistant pathogens and microbial infections. Related Articles | Metrics

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Biological activities of polysaccharides from Lachnum in different simulated digestive stages #br# #br# ZHANG Xinmiao, HE Yaling, YE Ming 2022, 39 (5):  52.  doi: 10.3969/j.issn.2095-1736.2022.05.052 Abstract ( 165 )   PDF   Save In order to explore the changes of components and biological activities of polysaccharides from Lachnum during the in vitro simulated digestion (saliva, gastric and intestinal). A novel extracellular polysaccharide (LEP-2a) was isolated and purified from Lachnum YM40. The digestive characteristics of LEP-2a, including the changes of molecular weight (Mw), chemical composition and micromorphology were evaluated by using in vitro simulated digestion model. The antioxidant activities of LEP-2a in different simulated digestive stages and its effect on lipid digestion were also measured. The results showed that LEP-2a was a heteroglycan with the Mw of 50.3 ku, which could not be digested by saliva, but the molecular weight decreased to 47.25 ku and 42.36 ku after in vitro simulated gastric and intestinal digestion, respectively. The reducing sugar content increased significantly and the atomic force microscopy showed that the height and branching degree of LEP-2a decreased significantly after gastrointestinal digestion. The results indicated that LEP-2a was degraded gradually mainly in gastrointestinal digestive stage. Besides, LEP-2a had stronger antioxidant activities after gastrointestinal digestion and could inhibit the decomposition of lipids effectively. These results provided a basis for further investigation of the in vivo activities of Lachnum polysaccharides. Related Articles | Metrics

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Effect of endophytes Bacillus polymyxa on degradation of pesticide residues in different parts of ginseng and accumulation of#br# ginsenoside monomers GAO Yugang, GAO Fei, TIAN Ying, WANG Xi, ZANG Pu 2022, 39 (5):  58.  doi: 10.3969/j.issn.2095-1736.2022.05.058 Abstract ( 139 )   PDF   Save In order to explore the effect of Bacillus polymyxa on the pesticide residues and ginsenoside content in different parts of ginseng, the pesticide residues and ginsenoside content in different parts of ginseng were determined by gas chromatography and high performance liquid chromatography before and after treatment with Bacillus polymyxa. The results showed that the degradation rates of Bacillus polymyxa on the five parts of pesticide residues in descending order were 87.25% of rhizome, 69.87% of fibrous roots, 69.02% of branch roots, 54.28% of xylem, and 32.69% of phloem. The degradation rate was only 7.58%-13.33% (P<0.05). The cumulative effects of Bacillus polymyxa on the content of the 9 ginsenosides in each part of ginseng in descending order were 21.34% fibrous root, 12.00% rhizome, 9.03% branch root, 8.60% phloem, 8.18% xylem (P<0.05). The results showed that the pesticide residues and ginsenoside content in different parts of ginseng had different distributions. Bacillus polymyxa had a significant effect on the degradation rate of pesticide residues in five parts and the content of 9 ginsenosides (P<0.05). Related Articles | Metrics

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Biodegradation of tylosin by intracellular crude enzyme solution of Klebsiella oxytoca ZHANG Tao, ZHANG Jin, SHI Yanke, WU Ronghao, FANG Xiaobo, ZHENG Huabao 2022, 39 (5):  65.  doi: 10.3969/j.issn.2095-1736.2022.05.065 Abstract ( 182 )   PDF   Save Intracellular crude enzyme solution of Klebsiella oxytoca (TYL-T1) was used as a starting material. The degradation characteristics of tylosin by TYL-T1 intracellular crude enzyme solution were studied. When the degradation system contains 25 mg/L of tylosin, the optimal degradation conditions were pH 7.0, 45 ℃, and 2.5 mg intracellular crude enzyme, resulting in 94.33% degradation rate within six hours. The addition of heavy metal ions such as Co2+, Fe2+, and Mn2+, respectively, promoted tylosin degradation. The process of tylosin degradation conformed to the first-order kinetic reaction model. The degradation rate constant was 0.714 9 h-1, the half-life was 1.0 h, and the maximum reaction rate was 22.58 mg/(L·h). The degradation products did not inhibit the growth of indicator bacteria, indicating that intracellular crude enzyme solution could eliminate the ecological toxicity of tylosin. Therefore, the intracellular crude enzyme solution of Klebsiella oxytoca might become an alternative tool to degrade tylosin. Related Articles | Metrics

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Inhibitory effect and mechanism of CS-MOF fiber on Microcystis aeruginosa #br# #br# DONG Zezhang, XUE Shuxin, CAO Jingguo 2022, 39 (5):  72.  doi: 10.3969/j.issn.2095-1736.2022.05.072 Abstract ( 183 )   PDF   Save In order to solve the problem of algae removal in water treatment plant, chitosan fiber and metal organic framework (MOF) were coupled to prepare a composite material (CS-MOF) with algae inhibition function. The effects of CS-MOF fiber on the growth, cell structure, photosynthesis, antioxidant enzyme activity and microcystin of Microcystis aeruginosa were studied. The results showed that CS-MOF fiber could effectively inhibit the growth of Microcystis aeruginosa, and the inhibition rate increased with the increase of dosage. When the dosage was 0.6 g/L, the inhibition rate of Microcystis aeruginosa could reach 85.6% on the 12th day. Scanning electron microscope showed that the algal cells in the treatment group were seriously damaged and the cell morphology changed significantly. During the experiment, the photosynthetic system of the treatment group was destroyed. The contents of chlorophyll a, carotenoid and phycobiliprotein in the algae decreased, and the inhibition rate of phycobiliprotein was significantly higher than that of chlorophyll a and carotenoid. CS-MOF fiber can destroy the antioxidant enzyme system of algae cells, and make algae cells suffer more serious damage by inhibiting SOD activity. In addition, CS-MOF fiber can effectively reduce the content of total algal toxin by inhibiting algal growth and adsorbing algal toxin. Related Articles | Metrics

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Accumlation of heavy metal between Lepisanthes rubiginosa and Sauropus androgynus in lead-zinc mining area #br# #br# CHEN Lin, YANG Xiaobo, YANG Ning, TIAN Lujia, LI Long, LIANG Caiqun 2022, 39 (5):  72.  doi: 10.3969/j.issn.2095-1736.2022.05.077 Abstract ( 99 )   PDF   Save In order to study the heavy metal enrichment capacity of two large biomass woody plants, Lepisanthes rubiginosa and Sauropus androgynus, they are planted in Changhua lead-zinc mine. The results showed as follows: the biomass of L. rubiginosa was 2.198 /hm2, and that of S. androgynus was 9.273 t/hm2. The contents of Cd, Pb, Cu and Zn in leaves of L. rubiginosa were higher than those in roots and stems. Under the same planting time, a total of Cd 6.64 g, Pb 22.76 g, Cu 12.72 g and Zn 542.23 g could be extracted from each hectare of soil by L. rubiginosa. S. androgynus could extracte Cd 306.28 g,Pb 92.82 g,Cu 63.02 g and Zn 12795.64 g Zn from each hectare of soil. Except that the enrichment factors of Pb and Cu in the stems and leaves of S. androgynus were lower than that of L. rubiginosa, the other enrichment coefficients were higher than that of L. rubiginosa. They can effectively adsorb these two heavy metals and transfer to their aboveground parts. Related Articles | Metrics

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Low nitrogen-induced ion balance and nitrate distributionin Populus euphratica Oliv. ZHANG Lin, SUN Yufang, GAO Yongfeng, YAO Yinan 2022, 39 (5):  82.  doi: 10.3969/j.issn.2095-1736.2022.05.082 Abstract ( 154 )   PDF   Save The potted control experiment method was used to analyze Populus euphratica Oliv. strategies to tolerate low nitrogen. The results showed that: Populus euphratica Oliv. plants have high carbon-nitrogen ratio to maintain a high nitrogen utilization rate; under low nitrogen stress, Populus euphratica Oliv. nitrates preferentially meet the shoots; roots and shoots have opposite nitrate assimilation rate-limiting enzymes (nitrate reductase). The activity change promoted the NO-3 transportation of the shoots and the transcription level of the NRT1.5C/NRT1.8B protein, which was responsible for the long-distance nitrate transportation of the xylem, further accelerated the NO-3 transportation of the shoots. At the same time, it was responsible for the transportation of nitrate from old leaves to young leaves. The increasing transcription level of the protein PeNRT1.7 accelerated the plant nitrogen nutrient turnover. Result showed that the root monovalent anions NO-3 and Cl- content greatly decreased under low nitrogen, and the K+ content of NO-3 long-distance transportation was reduced, resulting in a compensatory increase in the divalent ions SO2-4, Ca2+, and Mg2+, thereby ensured the plant ion balance. Therefore, Populus euphratica Oliv. could effectively adapt to moderate nitrogen stress through reasonable ion allocation and carbon and nitrogen balance strategies. Related Articles | Metrics

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Research progress of protein N-glycosylation modification in prokaryotes #br# #br# TAN Yahong, GAO Lijuan, SONG Wenxia, LU Xuemei 2022, 39 (5):  87.  doi: 10.3969/j.issn.2095-1736.2022.05.087 Abstract ( 685 )   PDF   Save N-glycosylation of proteins is the major post-translational modification occurring in nature and has important biological effects. It has been found that protein N-glycosylation is not restricted to eukaryotes, but also exists in prokaryotes. The research progress for prokaryotes protein N-glycosylation and the effects of glycosylation on the physiological function of organisms were summarized. The structure of N-glycosyltransferase and its recognition characteristics for substrates were also analyzed, and the application of the prokaryotic N-glycosylation system in glycosylation engineering was discussed, with a view to providing solutions for the design of new vaccines and disease treatments. Related Articles | Metrics

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Review on bacterial quorum sensing in marine phycosphere QIAO Zhen, LI Jialin, QIN Song 2022, 39 (5):  93.  doi: 10.3969/j.issn.2095-1736.2022.05.093 Abstract ( 200 )   PDF   Save Focused on the quorum sensing in the marine phycosphere, the signaling molecules types and structures were summarized, the biological functions governed by quorum sensing were explained, and their applications including biofouling prevention and algal bloom controlling were discussed. The future research should be focused on the improvement of the separation or identification techniques and the application of multi-omics analysis to further explore the mechanism and application potential of quorum sensing, and understand the complex relationship between algae and bacteria better. Related Articles | Metrics

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Neuroprotective activity and mechanism of the plant extracts LIAO Yang, YAN Rongling, HUAN Guowen, YIN Yeshi, LIU Xiaowen 2022, 39 (5):  98.  doi: 10.3969/j.issn.2095-1736.2022.05.098 Abstract ( 254 )   PDF   Save The research trends and existing achievements in the neuroprotective field of domestic plant extracts were summarized from these aspects such as the causes of neuronal injury. The active substance components, the typical manifestations, the underlying mechanism, the research trend and application prospect of domestic plant extracts were prospected in order to provide some reference for the further researches. Related Articles | Metrics

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Research progress on role of actin in virus infection LI Jie, ZHAO Shuling 2022, 39 (5):  104.  doi: 10.3969/j.issn.2095-1736.2022.05.104 Abstract ( 194 )   PDF   Save The research progress on the functions of actin in virus entry, replication, release and spread between the cells was summarized, which focused on the specific process and function of virus recruitment and induction of actin formation in different stages of virus infection as well as the molecular mechanism of virus regulating actin rearrangement. Although much is known about the involvement of actin in virus entry and release, there is still less research on its involvement in virus replication and assembly. The molecular mechanism and signal pathway of virus regulating actin need to be further studied in order to clarify the molecular mechanism of virus infection and develop new antiviral strategies. Related Articles | Metrics

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Optimization of cambial meristematic cells culture system of Taxus chinensis by response surface methodology #br# #br# XU Lingxia, WANG Jingyi, JIANG Jihong, CAO Xiaoying 2022, 39 (5):  108.  doi: 10.3969/j.issn.2095-1736.2022.05.108 Abstract ( 214 )   PDF   Save Taxus cells culture is one of the effective ways to produce paclitaxel, and the growth rate and induction of paclitaxel production of cambial meristematic cells are better than that of the ordinary cells. The effects of different mass concentrations of GA3, citric acid, ascorbic acid and activated carbon on the growth and browning rate of Taxus chinensis cambial meristematic cells were studied by response surface methodology (RSM) on the basis of single factor experiment. Response surface methodology analysis showed that the optimal mass concentration of GA3 as 0.5 mg/L, citric acid as 150 mg/L, ascorbic acid as 100 mg/L and activated carbon as 100 mg/L in the cambial meristematic cells basal medium could promote the proliferation and inhibit the browning of Taxus chinensis cambial meristematic cells. This study laid the foundation for Taxus chinensis cambial meristematic cells culture to produce paclitaxel. Related Articles | Metrics

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The path of ideological and political construction of courses in synthetic biology ZHU Hao, XIN Fengxue, ZHANG Wenming 2022, 39 (5):  116.  doi: 10.3969/j.issn.2095-1736.2022.05.116 Abstract ( 211 )   PDF   Save As a new interdisciplinary subject, synthetic biology is widely regarded as a subversive science and technology to change the future. Synthetic biology has important practical significance in the ideological and political construction of curriculum.Teaching students the expertise of synthetic biology requires combining synthetic biology with the fundamentals of Marxism to establish and guide the core values of Chinese socialism in the knowledge system of the curriculum. Scientific thinking and engineering ethics education is emphasized to improve students’ ability to identify, analyze and solve problems in the field of synthetic biology.Cultivating students’ self-confidence to climb the scientific peak and the responsibility to shoulder the mission of the times.On the practice path of the synthetic biology curriculum, it is necessary to further improve the teaching system, innovate the ideological and political form of the curriculum, and expand the height, depth, breadth and temperature of the ideological and political curriculum, making the synthetic biology curriculum and the ideological and political curriculum in the same direction, forming a synergistic effect, and realizing the “three complete education”. Related Articles | Metrics

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Exploration on the mode of ideological and political education in biochemistry curriculum from the perspective of scientific and research education NIE Huan, HE Jie, HUANG Xuemei, HAN Zhengbin, TIAN Weiming, LU Min, LIANG Lin, LI Yu 2022, 39 (5):  121.  doi: 10.3969/j.issn.2095-1736.2022.05.121 Abstract ( 221 )   PDF   Save Taking biochemistry as a case, this paper discusses the ideological and political education mode guided by “scientific research and education” and integrating “Curriculum” with “Ideological and political education”. By using the rapid development of scientific research in the field of biochemistry and taking scientific problems as the traction, we cultivating students’ comprehensive abilities such as independent learning, scientific thinking and daring to innovate. This mode tries to integrate ideological and political education into discipline development and professional knowledge. It improves students’ ideological and moral and scientific literacy and realizes the same frequency resonance of knowledge transfer and value guidance. And finally, it improves the effectiveness of ideological and political education. Related Articles | Metrics

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Reformation and practice on curriculum ideological and political education led by scientific research in immunology WANG Rui, DONG Lei, LYU Fang, ZHOU Lianjing, YAN Tianyi, LI Yujuan 2022, 39 (5):  125.  doi: 10.3969/j.issn.2095-1736.2022.05.125 Abstract ( 202 )   PDF   Save Taking the immunology as an example, the infiltrating curriculum ideological and political reforming practice led by scientific research was introduced. The curriculum ideological and political practice aiming to “cultivating scientific spirit and enhancing scientific research ability” was carried out in improving the teaching content and methods comprehensively. For example, the teachers practiced the scientific spirit of unremitting struggle, exploration, and innovation, by self-disciplined and progressive working, by patient and encouraging instruction to students, and by accessible, positive, and optimistic explaining to profound knowledge. Curriculum ideological and political elements combining with scientific research closely, such as the immune mechanism of anti-Corona Virus 2019 infection and other cutting edge of research advances, were used to cultivate dialectical thinking and strengthen innovation training. By completing the group work in form of research reports aiming to propose innovative ideas for major medical problems, the students’ scientific research abilities including analyzing thoroughly and deeply, questioning boldly, and teamworking was enhanced. Also, the students’s cientific spirit like being rigorousness and truth seeking, pursuing challenge and innovation were cultivated. The feedbacks from teaching peers and students showed that the multiform-curriculum ideological and political education of immunology focusing on scientific research played a positive role in guiding students’ world outlook and values from multiple perspectives, and achieved excellent teaching results. Related Articles | Metrics

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Exploration and practice of teaching reform of veterinary parasitology #br# #br# LIU Jing, LIU Qun 2022, 39 (5):  128.  doi: 10.3969/j.issn.2095-1736.2022.05.128 Abstract ( 206 )   PDF   Save Veterinary parasitology is a compulsory course for animal medicine majors. In recent years, with the change of farming patterns, the emergence of new diseases and the updating of teaching methods, the teaching target and social ecology are also changing. In order to adapt to these changes and development, the teaching syllabus were revised which adhered to the ideological and political education into the curriculum construction, with MOOC, virtual simulation teaching, herbarium of veterinary medicine and other teaching resources. Combin with the advantages of online and offline teaching, students’ main learning role was stimulated by introduction of the latest scientific research achievements and typical cases in the professional field to meet the needs of students to develop diversity. The feedback from the students showed that those reform methods increased their interest in learning veterinary parasitology, helped to create active classroom atmosphere and the teaching efficiency and quality. Related Articles | Metrics