Journal of Biology ›› 2022, Vol. 39 ›› Issue (5): 21-.doi: 10.3969/j.issn.2095-1736.2022.05.021

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Enterovirus 71 induces has-miR-200b expression to promote viral replication

  

  1. 1. Institutes of Biology and Medical Sciences, Soochow University,
    Suzhou 215123, China; 2. Department of Stomatology, Affiliated Suzhou Hospital of Nanjing Medical University,
    Suzhou Municipal Hospital, Suzhou 215001, China
  • Online:2022-10-18 Published:2022-10-18

Abstract: The role of miR-200b in EV71 replication was investigated by high-throughput sequencing, luciferase reporter gene assay and Western Blot. The results showed that the expression of has-miR-200b increased significantly in host cells after EV71 infection by high-throughput miRNA sequencing, and specific inhibition of miR-200b expression could inhibit EV71 replication. Luciferase reporter gene assay showed that miR-200b could directly target the 3′-UTR region of IFIT5. Further study confirmed that the increased expression of miR-200b induced by EV71 infection could inhibit IFIT5 expression and thus supress the type I interferon response. It is shown that EV71 infection can induce the increase of miR-200b expression in host cells and then inhibit the expression of IFIT5, resulting in the inhibition of intracellular interferon response, which facilitates EV71 replication.

Key words: EV71;hand-foot-and-mouth disease;miR-200b;IFIT5, interferon response;virus replication

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