Abstract: Based on the provincial bioengineering virtual simulation laboratory (VSL), we took molecular cloning and in vitro expression experiment of functional genes as an example to carry out the genetic engineering experiment teaching. An assessment and evaluation method combining virtual experiment with real experiment was constructed and practiced based on the principles of combining process with result and teacher evaluation with online and offline self-evaluation. The advantages of virtuality and reality combination in the gene engineering experiment teaching were assessed by comparing the students’ virtual operation and test score and investigating their satisfaction with this teaching mode. It was mainly embodied in the following four aspects: expanding the time and space of experimental learning and improving students’ learning effect; realizing the sharing of virtual and real resources and cultivating students’ information literacy; student-centered teaching and strengthening the ability of independent learning; diversified evaluation to achieve the purpose of promoting leaning by evaluation.

Key words: virtual simulation, innovation and exploration, classroom teaching mode, new training mode