Abstract: There are many problems in traditional experimental teaching of botany, such as difficult preservation of glass slides specimens, difficult observation of plant dynamic development, requirement of a large number of microscopes for the microstructure learning and difficult to master the operating process of paraffin section. In order to solve these problems, we have researched and constructed a botanical virtual slicing system independently. The system includes digital full-scale glass specimens scanned by the automatic microscope, 3D virtual simulation resources of paraffin technology, and auxiliary teaching resources such as video, animation, test questions database. This system can be used to realize the functions of online observation learning, autonomous learning, experimental report submission, virtual examination and online communication under the access of different types of terminal devices. Through the implementation of the experimental teaching mode of "combination of virtual reality and reality”, physical experimental teaching resources and virtual simulation experimental teaching resources have been organically combined. As a result, this new teaching mode not only enriches the content of experimental teaching, effectively expands the time and space of experimental teaching, but also sufficiently stimulates interest in learning，improves the effect of experimental teaching, and achieves the whole process coverage for experimental examination.

Key words: botany, virtual simulation, digital slides, experimental teaching