Table of Content

18 December 2016, Volume 33 Issue 6 Previous Issue    Next Issue

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Construction of chimeric single-chain antibody efficient soluble expression in Escherichia coli and optimization of fermentation conditions LIU Meng1,2,3, SUN Yang1,2,3, YANG Yan-kun1,2,3, BAI Zhong-hu1,2,3 2016, 33 (6):  1.  Abstract ( 314 )   PDF   Save Total RNA was extracted from the hybridoma cells which was prepared through human serum albumin immunized mice and reverse transcribed to cDNA by specific primers. Antibody fragments of VH and VL were amplified by RT-PCR and spliced with CH and CL genes of humanized Fab to construct a human-mouse chimeric single-chain antibody fragment (scFv). The scFv was cloned into pBY vector and transformed into E. coli BL21-trxB(DE3) cells for expression. Through the optimization of fermentation conditions, the soluble expression yield of scFv reached 1.23 g/L and 2.49 g/L in flask and 5 L fermenter respectively.  Related Articles | Metrics

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Analysis of N-linked glycans in tongue cancer GUO Hui1, ZHANG Jian3, YANG Gang-long2, HUANG Ru-jin2, GUO Jia2 2016, 33 (6):  7.  Abstract ( 307 )   PDF   Save In order to identify the N-glycans which are closely related with tongue cancer and provide the evidence to help the early diagnosis, prevention and treatment, the N-glycome in the serum of the healthy volunteers and patients with tongue cancer was compared. The human serum samples was collected and the N-glycans of the whole glycoprotein using N-glycan-FASP-T was isolated, and then identified and was their structures determined by MALDI-TOF/TOF tandem mass spectrometry. After that, the relationship between N-glycan abundance and the tongue cancer was analyzed by the method of ROC curve. Compared to healthy volunteers, in the serum of the patients with tongue cancer, the level of hybrid type, three-antenna and bisecting N-glycans are increased, while the two antennas and four antennas are N-glycans reduced. In addition, there are more terminal Neu5Gc. The statistical analysis results showed that 10 N-glycan structures were significantly upregulated in the course of tongue cancer (AUC>0.50, and AUC to 0.75, which m/z=1256.510/1418.573). Related Articles | Metrics

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Bioinformatics analysis and transcription difference of carotenoid isomerase (CRTISO) gene in Dunaliella viridis ZHOU Li-ya, GONG Yi-fu,ZHU Shuai-qi, WANG He-yu 2016, 33 (6):  15.  Abstract ( 341 )   PDF   Save Carotenoid isomerase (CRTISO) is a key enzyme in the pathway of carotenoid, which can catalyze the structure of carotenoids from cis-to trans-. Full length of crtiso in Dunadiella viridis was acquired by RNA-sequencing, then, the sequence was analyzed by several bioinformatics softwares. Transcriptional levels of D. viridis treated by different concentration of acetylsalicylic acid (ASA) and arachidonic acid (AA) were checked by real-time quantitative PCR. The full cDNA length of crtiso was 1176 bp. It contained a 795 bp open reading frame (ORF), encoding 264 amino acids. Relative molecular mass was 29 522.6. Theoretical isoelectric point of the protein was 5.86. It was predicted that the CRTISO in D. viridis was properly targeted to the cytoplasmic matrix. Compared with amino acid sequences in NCBI database, it was found that the amino acid sequence of CRTISO shared the highest homology with Chlorella variabilis (78%). RT-qPCR showed that the transcription level of crtiso reached the maximum when the concentration of AA got 62.5 mg/L and ASA reached 50 mg/L. The result of carotenoid content showed that the carotenoid content reached the maximum when the concentration of AA got 312.5 mg/L and ASA reached 100 mg/L. There was certain correlation between the expression of crtiso gene and the content of carotenoids. Related Articles | Metrics

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Analysis of gene sequence polymorphism in the sequences of Cyt b and D-loop in Nycticebus pygmaeus ZHU Wan-long1, CAI Jin-hong1, CHENG Jin-long2, WANG Zheng-kun1 2016, 33 (6):  20.  Abstract ( 233 )   PDF   Save Cyt b and D-loop of mitochondrial DNA in Nycticebus pygmaeus (sample=7) in Daweishan Nature Reserve from Yunnan province were PCR amplification in the present study, and the sequences of Cyt b and D-loop were tested, nucleotide compositions and polymorphism were analyzed. The results showed that in the gene of Cyt b, the proportion of four nucleotides(A, T, C and G) were 29.3%, 28.2%, 29.8% and 12.7%, respectively. Content of A+T was 57.5%, and content of G+C was 42.5%. In Cyt b gene sequences (1078 bp in length ), there were 4 variable sites, π value was 0.00106, only 3 haplotypes appeared in 7 samples. Genetic distance was 0.000-0.003 in N. pygmaeus. In the gene of D-loop, the proportions of four nucleotides(A, T, C and G) were 31.8%, 29.1%, 25.0% and 14.1%, respectively. Content of A+T was 60.9%, and content of G+C was 39.1%. In D-loop gene sequences (381 bp in length), there were 4 variable sites, π value was 0.00300, only 4 haplotypes appeared. Genetic distance was 0.000-0.003 in N. pygmaeus. All of the results suggested that litter differences existed between individuals within species, and had low levels of polymorphism in groups. Compared with other species, through the analysis of genetic diversity, N. pygmaeus showed lower hereditary and population numbers, genetic drift occured larger. Therefore, it was eager to protect the N. pygmaeus on the level of genetic diversity. Related Articles | Metrics

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Effect of cold stress on PI3K/AKT/GSK-3β signal pathway in fishes WANG Jing-feng1,2, HU Peng1,2, NIU Hong-bo1,2, CHEN Liang-biao1,2 2016, 33 (6):  24.  Abstract ( 398 )   PDF   Save To investigate the PI3K/AKT/GSK-3β signaling pathway in fish cold regulatory mechanism, three fish species, including tilapia (Oreochromis niloticus), zebrafish (Danio rerio) and grass carp (Ctenopharyngodon-idellus), were exposed to the cold temperature (8℃). They were reared at a common temperature (28℃) but had different cold tolerance (grass carp > zebrafish > tilapia).The expression of protein in the PI3K/AKT/GSK-3β pathway were examined. Results showed that the swimming performance decreased in turn: grass carp, zebrafish and tilapia. In tilapia, the expression of p-PTEN was decreased at cold temperature, whereas the expression of AKT, p-AKT(Ser 473) and p-GSK-3β(Ser 9) was increased at 8℃. Both in zebrafish and in grass carp, the expression of AKT, p-AKT(Ser 473) was repressed at 8℃, whereas the expression of p-GSK-3β(Ser 9) was induced at 8℃. Although the expression of p-GSK-3β(Ser 9) was commonly induced at cold temperature, and the increased abundance varied in the three fish species. The induction of p-GSK-3β(Ser 9) at 8℃ in tilapia was more significantly than that in other two fishes. PI3K/AKT/GSK-3β signaling pathway is involved in cold response and whose regulation vary in the fish species, which may contribute to the various cold tolerance of fishes. Related Articles | Metrics

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Research on immunogenicity of the epitope peptide of TonB-dependent receptor 1 of Riemerella anatipestifer LUO Ya-li,HUANG Chun,LI Pei,KONG Qing-ke 2016, 33 (6):  29.  Abstract ( 256 )   PDF   Save TonB-dependent receptor 1(TbdR1) was recently discovered to be an immunogenic antigen, which showed cross-reaction among R. anatipestifer serotypes 1, 2, and 10. Through bioinformatics analysis. Some of the epitope peptide of TonB-dependent receptor 1 of Riemerella anatipestifer were chosen and researched about their immunogenicity. The results showed that ducks can be protected to some extent by epitope peptide establishing a foundation of the research of the development of the subunit vaccine.      Related Articles | Metrics

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Construction and biological characterization of a rmlD mutant of Salmonella typhimurium HUANG Chun, LUO Ya-li, LI Pei, LIU Qing 2016, 33 (6):  33.  Abstract ( 305 )   PDF   Save To study the influence of rmlD deletion on phenotype and virulence of Salmonella typhimurium, S. typhimurium ΔrmlD mutant of S100 ΔrmlD was constructed by suicide plasmid-mediated homologous recombination. The biological characteristics of the ΔrmlD mutant showed that no difference was observed for the growth curve, sensitivity to polymyxin B and DOC of rmlD strain and the wild-type strain S100. However, the ability of adhesion and invasion was significantly increased compared to that of the wild-type strain. In addition, the rmlD mutant strain showed approximately 2000-fold decrease in virulence. In conclusion, deletion of rmlD gene had no effect on the growth and the sensitivity to polymyxin B or DOC, but significantly decreased the virulence. These data indicated that rmlD gene deletion could be an efficient way to reduce the virulence of Salmonella typhimurium for vaccine development.  Related Articles | Metrics

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The bacterial communities constitution and distribution in sediment of seagrass in Xincun Bay, South China Sea JIANG Yu-feng1,2,LING Juan1,DONG Jun-de1,3,ZHANG Yan-ying1,3 2016, 33 (6):  38.  Abstract ( 261 )   PDF   Save In order to compare the bacterial communities constitution and distribution in three sites of seagrass sediment in Xincun Bay, South China Sea, Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) analysis compounded with cloning and sequencing bands in DGGE profile was used. The results diverse bands of DGGE profile showed the most similarity of bacterial communities was found between the second and the third site in spring with an coefficient of 81.5%, whereas the least was 29.3% between the first and the third site in autumn. Shannon diversity indexes of the bacteria in spring and winter were higher than other seasons, which were of 3.03-3.07 and 3.23-3.25, respectively. Further, based on the 16S rDNA-V3 fragments from 42 individual DGGE bands and clone library sequence, the results showed most of the sequences belonged to Proteobactetia and Firmicutes. The most abundance Proteobactetia was Gammaproteobacteria with a percentage of 65.80%. Cyanobacteria were widely distributed in all samples. In addition, there were differences in bacterial communities among different seasons and sites. Psychrobacter and Pseudoalteromonas were advantage groups in the second and the third site during autumn, with a percentage of 31.61% and 19.41%, respectively. Related Articles | Metrics

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Research on effecting factors for reproduction and survivability of pollinating fig wasps in fig-fig wasps mutualism ZHANG Yuan1， DENG Guo-bin1， LI Zong-bo2 2016, 33 (6):  43.  Abstract ( 282 )   PDF   Save The interaction between pollinating fig wasps (Agaonidae) and their host fig trees (Ficus) is a striking example of an obligate pollination mutualism. Agaonidae resource is the key factor which hinges fig-fig wasps reproduction. To test the influences of seasonal change, length of female phase and foundress age on the wasps offspring numbers, abortion ratio and body length of wasps offspring, we performed manually-controlling experiments on two important Ficus species distributed in Xishuangbanna, Ficus semicordata and Ficus altissima. The experimental results revealed that seasonal change had no significant influence on wasps offspring numbers and abortion ratio, while it had significant impact on offspring body size. Furthermore, prominent negative influences can be found either along with the increase in age of foundress, or with the extension of female phase of figs, such as lower offspring numbers, higher abortive percentage and shorter body length of offspring. Related Articles | Metrics

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Effects of high carbonate alkalinity water and high ammonia water on oxygen consumption and ammonia excretion in Leuciscus waleckii Dybowski HE Qiang1,2, CHANG Yu-mei1, SU Bao-feng1, SUN Bo1, YAN Hao1,2, LIANG Li-qun1 2016, 33 (6):  48.  Abstract ( 217 )   PDF   Save Using hydrostatic water in sealed chamber for measurement, this study compared the change of oxygen consumption rate and ammonia excretion rate of Leuciscus waleckii Dybowski (72.62±3.70)g exposed in high carbonate alkalinity water (CA 50 mmol/L) and high ammonia water (NH4CO3 1.5 mmol/L) for 4 time points (2 h, 4 h, 6 h and 8 h) measurement. Oxygen consumption rate was significantly inhibited in carbonate alkalinity water and high ammonia water when compared that in fresh water (P<0.05). No significant difference of the oxygen consumption rate between carbonate alkalinity and ammonia water treated fish was detected for the last three tested time points, 4, 6, and 8 except for the first testing time point. Results of ammonia excretion rate showed that compared with those in fresh water, the ammonia excretion rate of fish exposed in carbonate alkalinity water and ammonia water was significantly inhibited (P<0.05). In ammonia water, the ammonia excretion rate of L. waleckii Dybowski for all 4 time points decreased, which had an extremely significant difference compared with fresh water and carbonate alkalinity water. Energy metabolism (O∶ N) study found that there had no significant difference in oxygen-nitrogen ratio in fish between fresh water and carbonate alkalinity water. However, compared with fish in fresh water and carbonate alkalinity water, fish in ammonia water had an extremely significant difference in oxygen-nitrogen ratio.  Related Articles | Metrics

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Evaluation of nutritional quality and proximate composition in the muscle of Pseudaspius leptocephalus in Ussuri River WU Shan-fu1,2, ZHANG Ying1, MA Bo1, ZHANG Yong-quan1, XU Wei1, SUN Da-jiang1 2016, 33 (6):  52.  Abstract ( 276 )   PDF   Save The nutritional quality and the proximate composition including the amino acid and fatty acid composition in muscle of wild P. leptocephalus in the Ussuri River were determined by routine methods. The results showed that the contents of moisture, crude protein, crude fat and ash in fresh muscle of P. leptocephalus were 77.98%, 19.05%, 1.66% and 1.20%, respectively. Eighteen kinds of amino acids were found in muscle of P. leptocephalus including 8 kinds of essential amino acids (EAA). In wet muscle, the content of total amino acids (TAA) was 19%, the content of EAA was 7.79%, 2 kinds of half essential amino acids (HEAA) were 1.63%, 8 kinds of nonessential amino acids (NEAA) were 9.57%; it was clear that the content of different amino acids was stable and the essential amino acid composition met the Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO) standards. Four kinds of delicious amino acids (DAA) accounted for 6.99% in wet muscle. The contents of EPA and DHA in wet muscle were 4.20% and 8.04%, respectively. According to nutrition evaluation in amino acids score (AAS) and chemical score (CS), the limited amino acid of P. leptocephalus was Val and Try, and the essential amino acids index (EAAI) of P. leptocephalus was 90.05. The results indicated that the contents of amino acids and high-unsaturated fatty acids in muscle of P. leptocephalus were higher, and the composition of amino acids was reasonable, thus, P. leptocephalus is a high quality freshwater fish with high nutritive value and health value, which has good prospects for exploitation and utilization.  Related Articles | Metrics

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Community structure and seasonal variation of macrozoobenthos in collapse lakes of Huaibei mining areas LI Xiao-ming, Ding Jian-hua 2016, 33 (6):  57.  Abstract ( 215 )   PDF   Save Seasonal variations in community structure of macrozoobenthos were investigated in collapsed lakes of Huaibei mining areas Liuqiao Lake and Linhuan Lake in September (autumn) and December (winter) of 2013, and in March (spring) and June (summer) of 2014. A total of 32 species belonging to 6 classes and 3 phylum were collected. The average densities were 210.956 ind/m2 in Liuqiao Lake and 181.65 ind/m2 in Linhuan Lake. The average biomass of macrozoobenthos were 60.55 g/m2 in Liuqiao Lake and 40.17 g/m2 in Linhuan Lake, respectively. The seasonal changes of Shannon－Weiner index (H′) and Margalef index (D) were same. The two indexes were all lowest in December, and higher in June and September. The seasonal change of Margalef index was more obvious (F(3, 16) > 3.95, P<0.05). The analyses of SIMPER and One-Way ANOSIM indicated that community structure of dominant species of macrozoobenthos was obviously different between Liuqiao Lake and Linhuan Lake. Related Articles | Metrics

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The community succession and structure characteristics of phytoplankton in Yangshan port LI Xiao-lin1,2, XUE Jun-zeng1,2, WU Hui-xian1,2 2016, 33 (6):  62.  Abstract ( 237 )   PDF   Save Diatom was the dominant taxa of phytoplankton with a total phytoplankton density of 66%-83% during the study period (2011-2013). Skeletonema costatum was the dominant species in diatom taxa (Y≥0.1), and the following dominant species was Paralia sulcata. The other major dominant species were nearshore protosalt species， including Cocconeis placentula, Coscinodiscus marginato-lineatus, C. granii, Nitzschia pungens and Thalassionema nitzschioides. There were more dominant species in winter, whose species number were greater than 10, whereas those were less in summer and autumn with a species number of 2-6. The seasonal variations of total nitrogen and total phosphorus concentrations were significant with a higher level in summer and lower level in winter. Analysis of variance indicated the difference of phytoplankton density between three years was significant (P＜0.05, F(2, 69)=6.22). The monthly average density of phytoplankton change each year without stable rule was related to the tidal changes in harbor. Pearson correlation analysis showed the diatom density had significant positive correlation with suspended solids and total phosphorus (P<0.01), thus phytoplankton density was affected by phosphorus greatly. Since the 1980s the density of phytoplankton increased significantly and the increasing rate of phytoplankton density was up to 103, 102 and 10 in 1980s, 1990s and after 2000s, respectively. The number of dominant species in dinoflagellates was increased and the species composition was also changed while the size of dominant species in diatom was minimized gradually. Compared to the historical data, the increment of phytoplankton density and the change of community structure were closely associated with eutrophication, imbalance of nutrient structure and phosphorus deficiency in Yangshan port. Related Articles | Metrics

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Study on community of Lindera obtusiloba in Yuntai Mountain of Jiangsu Province MENG Yan, LAI Yu, YANG Wen-jia, WANG Qing-ya 2016, 33 (6):  68.  Abstract ( 249 )   PDF   Save The aim of this study is to imestigate the community structure of Lindera obtusiloba completely for the first time, and the result showed that the community was of a clear vertical structure, including 300 species, belonging to 213 genera and 89 families in total. It was composed of arbor layer, shrub layer and herb layer, in which the importance value of Fagaceae, Lindera obtusiloba and Oplismenus undulatifolius was the largest, respectiuely. The rank distribution of Raunkiaer frequency was A>B>C>D>E. Among all layers of the community, Margalef richiness indexes were herb layer＞shrub layer>arbor layer, Simpson and Shannon-Wiener indexes were herb layer>arbor layer＞shrub layer, and Pielou evenness indexes were arbor layer>herb layer＞shrub layer. The floristic composition shows a transition from tropic to sub-tropic and temperate. Related Articles | Metrics

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Characteristics of starch grains in chloroplast of three alpine halophytes near the Qinghai Lake ZHOU Yong-hui, LIU Yu-ping, ZHANG Xiao-yu, LYU Ting, XU Rui, SU Xu 2016, 33 (6):  73.  Abstract ( 313 )   PDF   Save The ultrastructure of chloroplast in the mesophyll cells of three typical alpine halophytes including Potentilla anserina, Polygonum sibiricum and Chenopodium glaucum grown at low temperature and in salt marsh near the Qinghai Lake was studied by using the transmission electron microscope. The result showed that the subcellular structure did not differ between the three halophytes under non-stress condition, but there were a lot of starch grains in the chloroplast of three alpine halophytes, which were usually round or oval in shape arranged along the longitudinal axis of chloroplasts, and the whole starch grains were low in electron-density. Meanwhile, the thylakoid of chloroplasts swelled differently, and some of them even became into cisterna. It suggested that the variations of starch grains in the chloroplast of three alpine halophytes stated above were the result of the long-time stress under the adverse ecological factors such as low temperature, low air pressure, strong radiation, salt marsh, etc., and were an adaptation to the environment. Related Articles | Metrics

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Methane production and sulfurous gas release characteristics during anaerobic fermentation in blue-green algae systems HU Yue, CAO Jing, LI Yu-cheng, DOU Yue-qin, WANG Zhen-zhong 2016, 33 (6):  78.  Abstract ( 268 )   PDF   Save Anaerobic fermentation is a kind of effective resource utilizations of algae. However, the carbon-nitrogen ratio (C/N) of algae is low and the methane need to be purified, which are the difficulties for practicality of algae fermentation. Methane production from different blue algae systems and sulfurous gas concentrations were investigated in lab scale. The results showed that the total methane production from system with algae/straw/seed sludge ratio of 8/2/1 was the highest, with productive rate of 176 mL/g(VS). There were different sulfurous gases within the biogas. H2S concentration was the highest, with the maximum value of 14016.12 μL/L during 50 days of monitoring. H2S value was positively correlated with CH4S, C2H6S, C2H6S2 and C2H6S3, which indicated the higher H2S concentration, the higher CH4S, C2H6S, C2H6S2 and C2H6S3 concentrations. Related Articles | Metrics

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The biology function of ST6Gal-I in tumor ZHAO Yu-jie, WANG Shu-jing 2016, 33 (6):  82.  Abstract ( 391 )   PDF   Save Modification of sialic acid is an important form of glycosylation, which is correlated with the occurrence and development of carcinoma closely. Under the influence of saliva acyltransferase, sialic acid is transferred to galactose which is at the end of glycoprotein or glycolipid through glyosidic bond. It is reported that high expression of sialic acid on cell surface plays a vital role on oncogenesis and clinical prognostic. Nowadays, more and more researchers pay attention to the molecular research about antigen of sialic acid relating to the target of carcinoma formation. Studies have shown that in the process of carcinogenesis, the acyltransferase expression and activity are corresponding to the change of sugar chain structure in saliva acid. The role of ST6Gal-I in the development of tumor and the change of its expression and activity during oncogenesis were discussed in this review. Related Articles | Metrics

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Research progress in microbial community in biological treatment of dairy industry waste water KANG Xiao-hu, ZENG Xiao-ying, LI Shi-weng 2016, 33 (6):  86.  Abstract ( 243 )   PDF   Save Dairy industry waste water is characterized by high organic matter content, high ranges of the pollutants concentration and easily biodegraded. Hence, biological treatment process is a key technology used in the treatment of dairy industry waste water. While the pollutants are degraded by microorganisms developed in the waste water, it is the most important factor affecting efficiency of the treatment. In this article, the recent advancements on microbial diversity in the process of aerobic, anaerobic and the current status of bioaugmentation were discussed with the purpose of the process selection, operating parameters optimization. Related Articles | Metrics

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Research progress of pharmacologic actions of tanshinoneⅡA LI Xiao-xiao1, WU Xue-ling1, JIA Shi-liang1, DAI Xue-ling1,2, SUN Ya-xuan1 2016, 33 (6):  91.  Abstract ( 530 )   PDF   Save TanshinoneⅡA is the fat soluble diterpene quinone constituent separated and extracted from Chinese herbal medicine Salvia miltiorrhiza. Pharmacological studies showed that it plays an active role in the treatment of cardiovascular diseases, neuronal protection and the protection of ischemia reperfusion. Because tanshinoneⅡA has various biological activities, such as anti-inflammatory, antioxidant, anti-tumor, these actions could enlarge its range of applications. In this review, the pharmacological actions and the mechanism of tanshinoneⅡA were summarized to provide references for the further development and utilization of Salvia miltiorrhiza and tanshinoneⅡA, and to make the most of the medicinal value of Salvia miltiorrhiza and tanshinoneⅡA. Related Articles | Metrics

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Advance in alginate lyases LUO Dan-dan, XUE Yong-chang 2016, 33 (6):  95.  Abstract ( 419 )   PDF   Save Alginate lyases can catalyze the degradation of alginate, a complex copolymer of L-guluronate and its C5 epimer D-mannuronate, with β-elimination mechanism and yield alginate oligosaccharides. Alginate oligosaccharide has many kinds of biological activities, such as promoting growth, enhancing resistance in plant and bacteriostasis, so that they have attracted more and more people′s attention. In this paper some research progress in the classification, structure, function, enzymatic properties of alginate lyase and the application of alginate oligosaccharides were reviewed. It will provide a theoretical basis for its application in industrial production. Related Articles | Metrics

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Enhanced endo-β-1,3-glucanase produciton through morphology control of Trichoderma harzianum WANG Jian1, ZHENG Zhi-yong 1, ZHU Li2, ZHAN Xiao-bei 1 2016, 33 (6):  99.  Abstract ( 287 )   PDF   Save There is a close relationship between morphology of Trichoderma harzianum and endo-β-1,3-glucanase production. The culture conditions for good morphology of Trichoderma harzianumin was optimized by single factor experiment, and the result showed as follow: 100 mL medium in 500 mL Erlenmeyer flask, 5% (V/V) of inoculum volume. The initial pH of the medium was 6.5 and shaking speed was 180 r/min, 0.3%(m/V) Tween-80 or 0.3%(m/V) CMC-Na was added into the medium as dispersing agents. The mycelia pellet diameter was about (0.42±0.09) mm and the activity of endo-β-1,3-glucan was (111.9±7.5) U/mL. In the further orthogonal optimization, the activity of endo-β-1,3-glucan reached (135.2±8.3) U/mL, and the influence order of culture conditions to mycelia pellet diameter was inoculum volume >CMC-Na> Tween-80. Related Articles | Metrics

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Monoclonal antibodies production by hybridoma cell culture based on mixed medium model HUANG Ya-jie1,2, ZHU Guang-kai2, LIU Shan2, ZHAO Yong-qiang2, XIE Bo2, LIU Dao-jun1 2016, 33 (6):  104.  Abstract ( 399 )   PDF   Save In this study, hybridoma cell was adapted to the mixed medium containing less fetal bovine serum through suspension domestication, and the antibody concentration increased largely. The components of mixed medium were RPM1640∶ SFM4CHO∶ OptiCHO(4.5∶ 4.5∶ 1) with 2% FBS and the fed-batch fermentation process was carried out, in which the initial inoculation concentration was 3×105 cells/mL, and 3% (initial volume) chemical-defined feed medium was added every other day during cultivation. The antibody concentration increased to 168 mg/L from 84 mg/L. The hybridoma cells were further cultured in a 5 L stirred-bioreactor, and the products were purified by protein A affinity chromatography and the purified antibody concentration was 240 mg/L. Related Articles | Metrics

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The reform and practice for general education on modern biotechnology and human life GUO Xin-hong, HE Xiao-xiao, ZHOU Ling, ZHAN Tian-ming 2016, 33 (6):  110.  Abstract ( 309 )   PDF   Save Modern biotechnology and human life" is one of the core courses in our University. Its aim is to break the bulwark of the subjects and improve the biological quality of non-biological undergraduates. Based on general education, we explored its teaching reforms from different aspects, including teaching objectives, teaching contents, teaching methods and evaluation methods in view of its actual teaching conditions. The goal of stimulating students′ enthusiasm for learning and improving the quality of teaching was achieved by the curriculum reform. A valuable role in general education practice is expected.  Related Articles | Metrics

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Exploration and practice of clinical case-driven teaching pattern in medical cell biology LI Xiang 1,GUAN Feng 2 2016, 33 (6):  113.  Abstract ( 234 )   PDF   Save Based on the fundamental of medical cell biology, the teaching model of students-central and clinical case-driven teaching pattern was adapted. This model focused on students instead of teachers and on comprehensive quality training instead of accomplishing teaching tasks. It provids certain references for fundamental biology courses in medical schools. Related Articles | Metrics

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Exploration and practice of the reform of general biology curriculum QIU Le-quan, WU Tao, ZHAO Man, ZHONG Li, TANG Xiao-ling 2016, 33 (6):  116.  Abstract ( 320 )   PDF   Save General biology is one of the academic fundamental courses for undergraduate students in biological major. According to the characteristics of general biology course, combined with the requirements of personnel training in engineering colleges, this paper discussed the teaching reform in teaching contents, teaching methods, network assistant teaching and assessment method. Related Articles | Metrics

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The characteristics of the evolutional biology curriculum in institutions of higher learning and teaching ideas DUAN Yan-hong,WANG Xuan-nian 2016, 33 (6):  120.  Abstract ( 258 )   PDF   Save Evolutional biology course has the very big changes in recent years. This study introduced the characteristics of evolutional biology course and teaching ideas based on the summarization of biology education after many years, so as to provide certain ideas and thinking for helping younger Chinese workers in this aspect of the reform and research. Related Articles | Metrics

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The application and exploration of seminar combine with CBL teaching model in physiology teaching LI Min-yan 2016, 33 (6):  124.  Abstract ( 235 )   PDF   Save To explore the teaching methods of improving the teaching quality of medical physiology courses in higher vocational colleges, a suitable learning method for medical students was studied by introducing the typical cases of seminar + CBL method, and the effect was researched by stochastic comparison on the students of laboratory medicine of 2017 class and 2018 class on campus, and tracking the feedback and the final grade analyzed with SPSS17.0. Compared seminar+CBL with traditional teaching methods, there was a very significant difference in student′s grade with t test P﹤0.01. Analysis of the questionnaire showed that seminar+CBL teaching method is better to fit the job requirements and to improve students′ active learning ability.  Related Articles | Metrics

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Teaching reformation and exploration on identification of forest insect ZHAN Qing-bin 2016, 33 (6):  127.  Abstract ( 241 )   PDF   Save In order to improve students′ ability to identify rare insect and combat crime quickly for protecting forest resource and system, the curriculum for identification of forest insect was studied and reformed. We clarified the aim of the course and reformed curriculum content, teaching approach and assessment criteria. Related Articles | Metrics