Abstract: Biochemical analysis technology explored on “the integration of theory and practice, and three-dimensional teaching model incorporating teaching, learning, doing, thinking and using” based on the student-centered concept. Theoretical teaching focused on optimizing the design of teaching content and selecting teaching methods to help students understand difficult points in the course more easily with the help of abundant online teaching resources. During practical teaching, zero-based experimental operations and teaching blank space design has been used in the first class to strengthen the foundation of experiments, to enhance operational skills, to inspire investigative spirit, and to promote the return of the student’s dominance in learning. By expanding the second classroom, we extended the knowledge from the first classroom and elevated students’ abilities, cultivated their advanced abilities such as exploration and creativity, and achieved deep learning. Practice showed that the three-dimensional teaching model promoted the interconnection and integration of the first and second classrooms in terms of form and content, and effectively created a new way to curriculum and teaching reform. It has significantly improved students’ ability to independently conduct scientific experiments and laid a solid foundation for their future independent scientific research.

Key words: biochemical analysis technology, integration of theory and practice, three-dimensional teaching mode, practical operating ability cultivation