Abstract: The comprehensive experiment of fermentation technology is the core course for cultivating students’ innovative practice ability in the undergraduate major of biotechnology. With the progress of the discipline and the development of the industry, the original teaching system has problems such as limited content setting, lack of platform support, rigid organizational model, single assessment standard and imperfect experimental system, which affect the cultivation of engineering practice and innovation ability of professional talents. In response to solve those problems, the research and practice of the online and offline hybrid teaching system of the “five-integration” fermentation process comprehensive experiment of bioengineering undergraduates has been carried out, and good results have been achieved.

Key words: biotechnology, comprehensive experiment of fermentation technology, five fusion, hybrid teaching system, teaching practice