Journal of Biology ›› 2023, Vol. 40 ›› Issue (1): 123-.doi: 10.3969/j.issn.2095-1736.2023.01.123

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Design and exploration of a genetic experiment based on transposable mutation

LI Nan1, LI Yunfeng3, GUO Haibin1, LI Yajuan1, XU Ke1, LIU Ziqiang2   

  1. 1. Experimental Basis and Practical Training Center, South China Agricultural University, Guangzhou 510642,
    China; 2. College of Agriculture, South China Agricultural University, Guangzhou 510642, China; 3. College of
    Agronomy and Biotechnology, Southwest University 400716, China
  • Online:2023-02-18 Published:2023-02-21

Abstract: Transposable insertion mutantmf1shows more than two florets in one spikelet. Taking this mutant material as the starting point, a comprehensive genetic experiment was designed by combining the experimental contents of observation of rice young panicle differentiation and development, examination of spikelet morphological structure, genetic analysis and PCR detection. Through the integration of online virtual simulation experiment and offline practical learning, students can more intuitively understand the process of rice young panicle differentiation and development, comprehend the concept and application of transposable factors, clarify the relationship between wild type and mutant and between phenotype and genotype, and learn techniques and methods of plant microsection, PCR detection and variance analysis. The implementation of the experiment has important practical significance for cultivating students’ comprehensive application ability of multi-disciplinary knowledge and scientific research ability.

Key words: experimental teaching, comprehensive experiment, genetics, scientific research, virtual simulation experiment, transposable element

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