Abstract: Research feeding teaching (RFT), based on its advantages in many courses teaching, was applied in teaching of biological separation engineering， combining with experimental modes of comprehensive experimental teaching (CET) and open experimental teaching (OET), and then the teaching effects were analyzed through the test of theoretical knowledge and questionnaire of student investigation. The results showed that the experimental teaching mode of combining with RFT, CET and OET could effectively arouse students’ interesting to learn and communicate with others when compared to the models of OET and OET+CET, respectively. It could be beneficial for students to master the theory knowledge and improve the comprehensive application ability including: innovation, experimental operation, scientific paper writing and etc., and it could be worthy for popularization and application in the teaching of biological separation engineering and similar courses.

Key words: biological separation engineering, research feeding, comprehensive experimental, open experimental, teaching effect