Abstract: This study aimed to establish a technical system for tissue culture and rapid propagation for the forest-derived medicinal plantPicrasma quassioides, thereby providing technical references for the clonal propagation of superiorP. quassioidesindividuals and the development of new cultivars. By utilizing the sprouting shoots of eliteP. quassioidesplants as explants, this research investigated lateral bud proliferation technology and pinpointed the optimal conditions for adventitious bud induction, multiplication, rooting, and transplantation. Consequently, an efficient rapid propagation system forP. quassioideswas successfully developed. The findings indicated that the most effective medium for initial bud induction was A1 \[MS medium supplemented with Hyponex No.1 at 750 mg/L, Ca(NO3)2at 460 mg/L, and KH2PO4at 70 mg/L\]+6-BA at 0.4 mg/L+NAA at 0.5 mg/L+KT at 0.5 mg/L, achieving an induction rate of 97.71%. For subculture and proliferation, the optimal medium was A1+6-BA at 0.3 mg/L+NAA at 0.2 mg/L+IAA at 0.5 mg/L+KT at 0.3 mg/L, with a proliferation coefficient of 6.87. Regarding rooting, the best-performing medium was 1/2A1+IAA at 0.5 mg/L+IBA at 0.8 mg/L+activated carbon at 0.05 g/L, resulting in a rooting rate of 98.29% and an average of 5 roots per plant. Following acclimatization, the plants were transplanted into a substrate with yellow clay, vermiculite, and peat soil in a volume ratio of 3∶4∶3, achieving a transplantation survival rate of 97.41%.

Key words: Picrasma quassioides, tissue culture, rapid propagation, proliferation coefficient