Journal of Biology ›› 2026, Vol. 43 ›› Issue (1): 11-.doi: 10.3969/j.issn.2095-1736.2026.01.011

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A preliminary investigation of the methanogenic capability and mechanisms of the hyperthermophilic archaeon Geoglobus sp. DY224T3A5

CHEN Hongxin, LIU Delin, WANG Wanpeng   

  1. Key Laboratory of Marine Biological Genetic Resources, Third Institute of Oceanography,
    Ministry of Natural Resources, Xiamen 361000, China
  • Online:2026-02-18 Published:2026-02-27

Abstract: 以南海大西洋中脊深海硫化物区分离的超嗜热古菌Geoglobussp. DY224T3A5为对象,整合分子生物学与经典微生物学方法,系统探究其在不同浓度长链烷烃(C16~C32)底物下的产甲烷特性。实验数据表明,该菌株在中长链培养体系中可生成甲烷,全基因组测序揭示其环状染色体编码2177个功能基因,但缺乏产甲烷关键酶甲基辅酶M还原酶(MCR)的基因。通过基因组比较与代谢通路分析,提出“微型产甲烷”假说:在伍德—永达尔途径中,一氧化碳脱氢酶(CODH)可能介导N5-甲基四氢甲烷蝶呤(CH3-H4MPT)的甲基转移反应,通过脱甲基化作用生成微量甲烷。首次揭示Archaeoglobi纲古菌通过非典型途径参与烷烃耦联产甲烷的分子证据,不仅拓展了极端环境微生物代谢多样性的理论边界,更为深海油气藏生物气化技术及甲烷通量模型的优化提供了新范例。

Key words: geoglobus, methanogenic archaea, alkanes, 16S rRNA, metabolic pathway

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