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Screening, identification, and soil remediation of a 1 ,2-dichloroethane degrading bacterium YN5
YANG Zongzheng, WANG Lang, TANG Li’na, XIN Zonghan , WU Yi , WU Zhiguo
2026, 43 (1):
26.
doi: 10.3969/j.issn.2095-1736.2026.01.026
In this paper, a
bacterium YN5 capable of degrading 1,2-DCA was screened from soil
contaminated with 1,2-dichloroethane (1,2-DCA) in a chemical plant.
Through morphological observation, physiological and biochemical tests,
and 16S rRNA sequence analysis, the strain was preliminarily identified
asAncylobactersp.. The growth and degradation characteristics of strain YN5 were
investigated by single factor experiment, and the strain was applied to
the remediation of contaminated soil. The experimental results showed
that strain YN5 could degrade 1,2-DCA effectively under the conditions
of pH 7－10, NaCl mass concentration 1－10 g/L, and temperature 20－40 ℃.
Under the optimal condition (temperature 30 ℃, pH 9, NaCl mass
concentration 1 g/L), YN5 could completely degrade 200 mg/L of 1,2-DCA
within 16 h. Under laboratory simulation conditions, the 2-day removal
rate of YN5 on 130 mg/kg 1,2-DCA contaminated soil reached 77.10%. It
was found that soil moisture content and ambient temperature
significantly affected the remediation effect of strain YN5. In
addition, the soil enzyme activity after YN5 restoration was
significantly improved, with the urease activity showing the most
obvious improvement, increasing by 130.83%. Strain YN5 had the ability
to efficiently degrade 1,2-DCA efficiently, showing potential
application prospects in the remediation of 1,2-DCA contaminated soil.
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