Abstract: Comparative teaching method presenting advantage and superiority in many courses teaching was applied in experiment teaching of biological separation engineering, and the teaching effects were evaluated on the questionnaire of student investigation. The results showed that the comparative teaching method could effectively arouse students’ interests, and promote the understanding and mastery of experimental principles and operation methods in experimental projects. It played an important role in improving students’ hands-on ability, exercising experimental operation skills, and cultivating innovative thinking abilities through the comparative learning of biomaterial selection, active substance extraction and isolation, and antioxidant capacity determination, which fully exhibited the superiority of this teaching mode in the experiment teaching of biological separation engineering. Therefore, the generalization of comparative teaching method could provide references for the experimental teaching design and reform of biological separation engineering and similar courses.

Key words: biological separation engineering, comparative teaching method, experiment teaching, exploration practice, teaching effect