Abstract:

Peroxidase isozymes of pea (Pisum sativum L.) from arious geographical origins were analyzed using native polyacrylamide gel electrophoresis. Based on the zymography characteristics of cultivars tested，similarity coefficient and phylogenetic relationships among pea (Pisum sativum L.) were obtained. The results showed that there were differences in band number, the enzyme content, enzyme activity of peroxidase isoenzymes from 23 pea cultivars. A total of 161 peroxidase bands and 14 types were observed and two major enzymes were presented. There were significant differences in band number among the cultivars, with the largest number being 8 and smallest 5, and Rf value ranging from 0.13 to 0.83.The genetic similarity of 23 pea cultivars was between 0.33-0.97. The analysis of zymography bands cluster showed that 23 pea cultivars could be divided into five groups. As the result, a certain correlation was observed between the classification result, the geographic location and source of the cultivars. Specifically,No.3 and No.17 were nearest to each other as the case of No.8 and No.16; No.12 and No.13, and No.6 was farthest to all the other cultivars.

Key words: Pisum sativum L., polyacrylamide gel electrophoresis, genetic relationship, cluster analysis, mobility