Abstract: As a core curriculum in life science，medicine，agronomy，computer science and some related fields，bioinformatics possess the interdisciplinary and frontier characteristics which brings some troubles to undergraduate teaching，for example，textbooks can not cover the latest important research results，and the depth and breadth of teaching content selection are difficult to grasp. In recent years，the growing concern has been paid to the construction of mobile microlearning resources and the integration of microlearning and traditional classroom teaching，and good results has been achieved. In this study，we conducted a survey research on the microlearning needs of the students who completed the course learning，and constructed a mobile microlearning resource of bioinformatics according to the theory of mobile microlearning，then used in the actual classroom teaching. We chose two basic chapters in bioinformatics teaching，and adopted two kinds of blended learning modes，i.e. station rotation and individual rotation models to explore a blended learning approach of bioinformatics supported by mobile microlearning resource. Our study provided a reference to the teachers of bioinformatics and related disciplines in colleges and universities.

Key words: bioinformatics, interdiscipline, blended learning, mobile microlearning