Abstract: With the continuous expansion of the scale of professional master’s degree students，ensuring the quality of professional master’s cultivation has become a major issue faced by high⁃level personnel cultivation in colleges and universities. The purpose of cultivating postgraduates with professional degree of subject teaching（biology）is to improve the quality of reserve force of biology teachers in middle school，so that they can master modern education theory，have strong research ability and have rich teaching practice. In order to improve the quality of the cultivation of postgraduates in subject teaching（biology），this paper analyzes the existing problems and their causes in the cultivation process of the two⁃year discipline teaching（biology）master，and puts forward some pertinent guiding measures and strategies，which has strong practical significance.

Key words: subject teaching（biology）, professional degree, cultivation quality, reason, countermeasure