Abstract: The background and the significance of the research on extracellular electron transfer (EET) in electricigens were introduced. The roles of various molecules such as cytochrome c, pili proteins and electron mediators involved in the EET process were summarized, and the relevant works on the use of network methods and omics methods to study the EET process of electricigens in recent years were reviewed. The applications of network analysis methods in the identification of key electron transfer molecules, mining of electron transfer modules and inference of electron transfer pathways were summarized from several aspects, such as protein networks, regulatory networks and integrated networks; the researches on the use of omics methods to identify electron transfer genes and their functional analysis were summarized from several aspects, such as genome, transcriptome, proteome, multi-omics and meta-omics; and the integration of biological networks and omics data to study the coordinated utilization of biomolecules, identification of key genes and gene clusters in the EET process of electricigens were also introduced. The current problems were discussed, and the future research directions of integrating multiple biological networks and multi-omics data to carry out EET research on electricigens were prospected.

Key words: bioinformatics, biological networks, omics methods, extracellular electron transfer, electricigens