Table of Content

18 February 2024, Volume 41 Issue 1 Previous Issue   

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Marine biotechnology for the “blue seed industry” QIN Song, SUN Lin, WANG Yajun, LIU Zhengyi, WANG Yinchu, ZHANG Jian 2024, 41 (1):  1.  doi: 10.3969/j.issn.2095-1736.2024.01.001 Abstract ( 137 )   PDF   Save The article reviews the theoretical and practical innovations of marine biotechnology in China over the past 30 years, from the perspective of the cutting across development of China’s “blue seed industry”. It also analyses the international development trends and the urgent needs for the sustainable and healthy development of China’s mariculture industry, and concludes that regionally responsive precision breeding for global changes is the direction of marine biotechnology for “blue agriculture” in the coming decade. Related Articles | Metrics

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Engineering of a carboxylic acid reductase-in the synthesis of pharmaceutical intermediate (S)-2-aminobutanol ZHANG Xiaohui, QIN Zongmin, LI Congcong, LU Fuping, QU Ge, SUN Zhoutong 2024, 41 (1):  6.  doi: 10.3969/j.issn.2095-1736.2024.01.006 Abstract ( 96 )   PDF   Save Armed with both hydroxyl and amino groups, (S)-2-aminobutanol is an essential chiral building block used in the preparation of diverse pharmaceutical drugs, while effective biocatalysts are highly desired toward the synthesis of (S)-2-aminobutanol. The carboxylic acid reductase derived from Segniliparus rugosus (SrCAR) was used as the model enzyme in the transformation of (S)-2-aminobutyric acid to (S)-2-aminobutanol combined with a heterologous alcohol dehydrogenase. After screening the previously constructed SrCAR mutant libraries, the active site was obtained and co-evolution analysis was performed. By using the combinatorial active-site saturation test strategy, the dominant mutant XH7 (G430V/E533F/A627N) was obtained. The kcat/Kmand Tmvalues were improved 2.1 fold and 2.3 ℃ compared to that of the wild type enzyme, respectively in the transformation of N-Boc-(S)-2-aminobutyric acid to N-Boc-(S)-2-aminobutyraldehyde. Further, N-Boc-(S)-2-aminobutyraldehyde to the alcohol product can be reduced by the introduction of Pseudomonas fluorescens (PfADH). The bi-enzymatic system containing of XH7 and PfADH enabled a 99% conversion toward 20 mmol/L substrate in 5 h. After deprotection and purification, the yield of (S)-2-aminobutanol reached 60%. Molecular dynamics simulations were performed to shed light on the molecular basis for the improved activity and thermostability of the best mutant. This study expands the biocatalytic toolbox for the synthesis of (S)-2-aminobutanol, and provides guidance for the biosynthesis of similar high value-added pharmaceutical intermediates. Related Articles | Metrics

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Heterologous expression, purification and enzymatic properties of dopa decarboxylase from Pseudomonas ZHOU Yaolin, SUN Dengyue, ZENG Zhixiong, LI Xia 2024, 41 (1):  14.  doi: 10.3969/j.issn.2095-1736.2024.01.014 Abstract ( 75 )   PDF   Save The dopamine decarboxylase (DODC) gene sequence from Pseudomonas was PCR amplified, double digested and ligated to the vector CV6-pGEX-6P-1, and the expression vector CV6-pGEX-6P-1-DODC was successfully constructed by validation and sequencing. Escherichia coli BL21 (DE3) was transferred for recombinant expression. The OD value was 0.6-0.8, the final concentration of isopropyl β-D-1-thiogalactoside (IPTG) was 0.1 mmol/L, and cultured at 16 ℃ overnight for 12-16 h. The results showed that DODC fusion protein with high expression was obtained in E.coli BL21(DE3) by induction expression. DODC purified protein with purity above 95% was obtained by GST-affinity chromatography, 3C protease digestion and ion exchange chromatography. The enzymatic properties of DODC were studied. The optimum reaction temperature of the enzyme was 40 ℃, which was sensitive to the effect of temperature, and the enzyme activity was more than 80% at 20-30 ℃. The enzyme activity decreased substantially above 30 ℃. The optimal buffer solution was PBS buffer solution, the optimal reaction pH was 7.5, the optimal substrate was L-DOPA. The metal cation Ca2+ promoted the enzyme activity. The sequence homology analysis showed that DODC from Pseudomonas belongs to the AAT-Ⅰ superfamily, and the conserved catalytic active site of the enzyme was predicted to be Thr 241. Related Articles | Metrics

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Anti-respiratory syncytial virus activity evaluation via primary human airway epithelial cell culture DING Huiru, ZHAO Min, CHENG Ningning, FU Yuanhui, PENG Xianglei, YU Jiemei, ZHENG Yanpeng, HE Jinsheng 2024, 41 (1):  20.  doi: 10.3969/j.issn.2095-1736.2024.01.020 Abstract ( 43 )   PDF   Save This study aims to establish the culture method of human primary airway epithelial cell (hAEC) and to investigate the anti-respiratory syncytial virus (RSV) activity and mechanism of 3-thioindole compound RSVA-4 and immunosuppressive metabolite 6-MMPR using hAEC system, which intends to construct a cell model for RSV drug screening and efficacy evaluation. The respiratory tract epithelial cells from volunteers were collected and cultured, then the morphology, activity and purity were identified. The anti-RSV activity and cytotoxicity of RSVA-4 and 6-MMPR were further verified in hAEC system. The mechanism of RSVA-4 and 6-MMPR accounting for the suppression of RSV replication on hAEC was explored by using fluorescence real-time quantitative PCR (RT-qPCR) and time-of-addition assay. The survival rate of cultured hAEC was 93.51% as determined by trypan blue staining. The half maximal inhibitory concentrations (IC50) of RSV-A-4 and 6-MMPR were （207.30±4.77） μmol/L and （3191.00±6.11） μmol/L, respectively. The half maximal cytotoxic concentration (CC50) of 6-MMPR was （95526.00±10.97） μmol/L, while no toxicity of RSVA-4 was observed on hAEC. Mechanistically, RSV-A-4 and 6-MMPr inhibited RSV replication in the genome replication/transcription phase. The hAEC culture method was successfully established, which could be used to screen and evaluate the anti-RSV drugs in vitro. RSVA-4 and 6-MMPR could effectively inhibit RSV replication at the cellular level. Altogether, the result could provide an experimental basis for the research and development of RSV drug and pathogenesis. Related Articles | Metrics

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The analysis of somatic and dendritic morphology of cerebellar Purkinje cells in mice at different ages PEI Pei, TANG Zhengquan 2024, 41 (1):  26.  doi: 10.3969/j.issn.2095-1736 Abstract ( 72 )   PDF   Save In order to investigate the difference in morphology of cerebellar Purkinje cells in mice at different ages, mice were divided into four age groups of five animals each corresponding to 14-day, 1-month, 2-month, and 1-year old mice. Parvalbumin selected as a unique marker for Purkinje cells, using immunochemistry, we observed and compared somatic and dendritic morphology of cerebellar Purkinje cells of these mice in four groups under a confocal microscope. The results showed that the dendritic length of cerebellar Purkinje cells in 2-month old mice was significantly higher than that in other groups (P<0.05), and the dendritic length of cerebellar Purkinje cells in 1-year old mice was significantly lower than that in other groups (P<0.05), as well as the somatic sizes of Purkinje cells of mice in 4 age groups showed a decreasing trend with age. In this study, we also analyzed the fluorescence intensity of parvalbumin in cerebellar Purkinje cells of mice in 4 age groups, which showed an overall increasing trend with age. Together, the results indicate that cell body size and dendritic length of cerebellar Purkinje are associated with age, with an opposite trend of parvalbumin expression and its development. This study suggests that parvalbumin may play an important role in regulation of cerebellar Purkinje cell development. Related Articles | Metrics

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Identification of key enzyme genes involved in lupeol synthesis pathway in Syneilesis aconitifolia by transcriptome analysis ZHANG Jingjing, XU Jingyao, SHAN Tingyu, ZHAO Liqiang, ZHONG Xinxin, ZHANG Shuaishuai, WU Jiawen 2024, 41 (1):  32.  doi: 10.3969/j.issn.2095-1736.2024.01.032 Abstract ( 62 )   PDF   Save order to analyze the lupeol biosynthetic pathway in Syneilesis aconitifolia and explore its key enzyme genes, DNBSEQ sequencing platform was used to sequence the transcriptome of leaves, stems, roots and rhizomes of Syneilesis aconitifolia, and 191 541 Unigenes were obtained after de novo assembly. KEGG metabolic pathway analysis showed that 961 Unigenes were involved in the lupeol biosynthetic pathway in Syneilesis aconitifolia, of which 395 Unigenes encoded 17 key enzymes of the biosynthetic pathway. Comparing root with other tissues, 24 shared differentially expressed genes were involved in the lupeol biosynthetic pathway, encoding farnesyl diphosphate synthase (FPPS), squalene synthase (SS), squalene epoxidase (SE) and other key enzymes. Structural analysis of the key enzymes FPPS, SS and SE, showed that they all had conserved catalytic domains and substrate binding domains. This work enriched the functional gene database of Syneilesis aconitifolia, and laid a foundation for further study of the lupeol biosynthetic pathway and the function and regulation mechanism of key enzyme genes. Related Articles | Metrics

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The anti-inflammatory, antioxidant activity test and GC-MS analysis of oil extracted from Vespa affinis CHANG Yaping, LIU Chaohe, XIAO Huai, ZHANG Chenggui, YANG Yinhe, YANG Dasong 2024, 41 (1):  41.  doi: 10.3969/j.issn.2095-1736.2024.01.041 Abstract ( 43 )   PDF   Save The oil was extracted from Vespa affinis firstly and divided into three parts based on polarity differences in this work. Its antioxidant activity was then tested by using the DPPH method, while its anti-inflammatory activity was tested in vitro using a mouse macrophage inflammation model induced by LPS. In order to clarify the chemical composition responsible for the activity, a GC-MS analysis of oil extracts were further performed. The results indicated that the antioxidant and anti-inflammatory active components were mainly concentrated in the large polar part. The oil from V. affinis was rich in unsaturated fatty acids, which are high quality nutrients. As the distinguishing and main components in large polar part, eugenol and linolenic acid are key compounds for the antioxidant and anti-inflammatory activity of oil from V.affinis. The completion of this research not only enriched the understanding about the chemical constituent and biological activity of wasp oil, but also laid the foundation for its further development. Related Articles | Metrics

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Study on the protective mechanism of lavender essential oil against ultraviolet damage of skin barrier LIU Fei, WANG Qian, HAN Tingting, YANG Suzhen, WANG Wencui, GUO Fengjiao 2024, 41 (1):  46.  doi: 10.3969/j.issn.2095-1736.2024.01.046 Abstract ( 49 )   PDF   Save This study investigated the protective effects and mechanisms of lavender essential oil (LEO) on ultraviolet light induced damage of skin barrier. The components of LEO were identified and analyzed by gas chromatography-mass spectrometry (GC-MS). 3D epidermal skin models were used to establish skin barrier damaged model by ultraviolet B (UVB) irritation at a dose of 600 mJ/cm2. The non-medication without UVB exposure group, non-medication with UVB exposure group, vitamin E/ dexamethasone treatment with UVB exposure group and LEO treatment with UVB exposure group were set up parallelly. The effects of different treatment on oxidative stress, inflammation, barrier structure-related proteins and CD44 receptor were investigated by hematoxylin-eosin staining, enzyme-linked immunosorbent assay, immunohistochemistry and immunofluorescence methods, etc. The results showed that a total of 51 chemical components of LEO were identified by GC-MS, and the main components were linalyl acetate, linalool, lavandulyl acetate, etc. UVB could cause damage to skin barrier exhibiting increasing ROS level and expression of inflammatory factors and decreasing SOD enzyme activity and expression of barrier-related proteins and CD44 receptors. LEO had a certain protective effect on UVB-induced skin barrier damage mainly by inhibiting UVB-induced increase in the expressions of IL-1α, TNF-α and PGE2 and ROS production (P<0.05) and by increasing the expression of four barrier-related proteins and CD44 receptor, and SOD enzyme activity (P<0.01) in the skin barrier damaged models. These results indicated that LEO had a great application prospect in reducing UVB damage of skin barrier, which provided basis for its application in skin care products development. Related Articles | Metrics

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The mitochondrial genome and phylogenetic analysis of Narosa fulgens (Leech, 1889) LIN Xingyu, SONG Nan 2024, 41 (1):  54.  doi: 10.3969/j.issn.2095-1736.2024.01.054 Abstract ( 42 )   PDF   Save To explore the mitochondrial genome of Narosa fulgens(Leech, 1889) and the phylogeny of Limacodidae,we newly sequenced the complete mitochondrial genome of N. fulgens(Lepidoptera, Limacodidae), by using the next-generation sequencing method. In phylogenetic analyses, we selected 16 exemplars of Zygaenoidea as ingroups. Each onespecies of Cossidae and Thyrididae was used as outgroup. We reconstructed the phylogenetic relationships using Maximum likelihood and Bayesian inference methods. The sequenced genome is a circular molecule of 15296 bp in length (GenBank accession No. OP919326) which possesses 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and putative control regions as well. For most of the protein-coding genes, the star codons were ATN except forcox1using CGA as star codon. Most of the protein-coding genes terminated with the stop codon TAA, whilecox2andnad4employed the incomplete T as the stop codon.All tRNA genes can be folded into atypical cloverleaf structure, with the except of thetrnS1-AGN. ThetrnS1lacks the DHU arm.The lengths of the rrnL and rrnS genes were 1335 bp and 732 bp, respectively, with the A + T contents of 85.24% and 84.70%. Both phylogenetic inference methods showed largely identical tree topological structures: the families Limacodidae and Zygaenidae were respectively monophyletic, Narosa fulgens is sister to Narosa nigrisigna. Related Articles | Metrics

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The serum metabolome analysis during multiple ovulation in sheep #br# FENG Rui, XU Quanzhong, WANG Chunwei, WANG Lequn, FENG Shuang, ZHANG Hailong, ZHANG Li, ZHANG Liguo, SU Xiaohu 2024, 41 (1):  61.  doi: 10.3969/j.issn.2095-1736.2024.01.061 Abstract ( 36 )   PDF   Save The object of this study is to investigate the changes of serum metabolites during multiple ovulation in sheep and look for metabolites related to follicular maturation. In this study, the untargeted metabolomics analysis was processed through LC-MS/MS to analyze the metabolites in the serum of donor sheep after simultaneous oestrus, before FSH injection (1st) and before artificial insemination (AI) (2nd). The metabolomics analysis showed that 1 158 metabolites (721 in positive iron mode and 437 in negative iron mode) were detected and 617 were annotated. The diferential metabolites were screened based on the following criteria: VIP≥1; FC>1.2 or＜0.83; P≤0.05. A total of 295 metabolites were differentially expressed between 2nd and 1st (186 up-regulated and 109 down-regulated). KEGG pathway enrichment showed that 88 functional pathways were enriched in IE groups, including steroid hormone biosynthesis, arachidonic acid metabolism and fatty acid biosynthesis. This study showed that high content of deoxycorticosterone, phosphatidylcholine and arachidonic acid in serum would be helpful for follicular developing in multiple ovulation. The results would help to explain the relation between blood material and follicular development and provide a theoretical basis for the nutrition regulation and donor selection in multiple ovulation. Related Articles | Metrics

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Antiviral components of Salvia tiliifolia against tobacco mosaic virus WANG Zifu, YAN Junyi, LIU Yang, WU Jiangmei, YAN Xiaohui, HU Shijun 2024, 41 (1):  68.  doi: 10.3969/j.issn.2095-1736.2024.01.068 Abstract ( 51 )   PDF   Save In order to find new plant virus inhibitors, tobacco mosaic virus (TMV) was used as the test virus. It was found that the extract of Salvia tiliifolia, an invasive plant, showed anti-TMV activity. Screening by the half-leaf method along with different treatment pattern of extract, the petroleum ether extract showed the strongest protective effect and inactivation effect at the concentration of 1 mg/mL, with inhibition rate of 40.95% and 52.85% respectively. The ethyl acetate extract showed the best curative effect with the inhibition rate of 52.85%, which was not significantly different from Ningnanmycin, a positive control. Among fractions of petroleum ether extracts, Fr7 showed the best protective effect and curative effect, with the inhibition rate of 40.51% and 45.52% respectively, and Fr6 showed potent inactive effect with the inhibition rate of 74.63%. Among fractions of ethyl acetate extracts, Fr1′ showed the strongest protective effect with an inhibition rate of 61.77%, Fr3′ displayed the best curative effect with an inhibition rate of 55.02%, and Fr4′ showed potent inactive effect with the inhibition rate of 60.22%. Five compounds were separated and identified by modern chromatographic spectrum technologies, they were tilifodiolide, eupatoric acid, ursolic acid, oleanolic acid, and 2-methyl-5,7-dioxy chromone. Related Articles | Metrics

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Effects of carbon ion beam irradiation on the growth, physiological characteristics of potato tissue culture plantlets 2024, 41 (1):  73.  doi: 10.3969/j.issn.2095-1736.2024.01.073 Abstract ( 38 )   PDF   Save To explore the effect of high-energy carbon ion beam on the stem section of potato tissue culture seedlings, this study took the stem section of potato variety “Xindaping” as the research material, four high-energy carbon ion beams with radiation dose gradient were selected. The plant lethal rate, rooting rate, lateral bud formation rate, morphological variation rate and leaf physiological and biochemical indicators were measured after irradiated. The semi-lethal radiation dose of the stem section of “Xindaping” tissue culture seedlings was determined. The results showed that there was a positive correlation between irradiation dose and lethal rate. The phenotypic changes of regenerated plants in stem segments of tissue culture seedlings were mainly as follows: asymmetric leaves, irregular leaf margins, variant elongation of stems, clustered leaves and adhesion of leaves. With the increase of irradiation dose, the activities of superoxide dismutase, peroxidase and catalase increased first and then decreased. The content of malondialdehyde increased first, then decreased, and last increased again. The semi-lethal irradiation dose of “Xindaping” was calculated by linear regression equation to be 20.37 Gy. Related Articles | Metrics

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A newly recorded species of Balanophoraceae from Fujian Province LIN Guican, QIU Qi, AN Chang 2024, 41 (1):  79.  doi: 10.3969/j.issn.2095-1736.2024.01.079 Abstract ( 47 )   PDF   Save In October 2022, Balanophora polyandra, a new record species of Balanophora in Fujian Province, was found in Huafeng Town, Hua’an County, Fujian Province. The voucher specimens are deposited in the Herbarium of College of Life Sciences, Fujian Normal University. The morphology of this species is similar to that of three species of Balanophoraceae native to Fujian, but its medicinal stamens are nearly disc-shaped, with umbilicus-like protrusions in the center, short anthers, and the drug chamber is divided into 20-60 small chambers. The main morphological characteristics of the plant and its folk medicinal value were described in detail, and the habitat photos of the original plant were provided, which further supplemented the background information of vascular plants in Fujian Province and enriched the species diversity of Fujian Province. Related Articles | Metrics

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Spatial characteristics and driving factors of nitrogen and phosphorus in surface sediments of Xingyun Lake based on the machine learning KNN method XIONG Jing, YIN Pengfei, JIA Yuxin, YIN Jiqing, ZHANG Wenxiang 2024, 41 (1):  82.  doi: 10.3969/j.issn.2095-1736.2024.01.082 Abstract ( 44 )   PDF   Save Based on the experimental determination of nitrogen and phosphorus contents in 23 surface sediments of Xingyun Lake, combined with the nutrient data of the lake in different periods and the methods of machine learning K-Nearest Neighbor ( KNN ) and Inverse Distance Weight ( IDW ), Ordinary Kriging ( OK ) and Kernel Smoothing ( KS ) methods, the spatial distribution characteristics of nitrogen and phosphorus contents in surface sediments and the prediction accuracy of each interpolation model were analyzed. The influencing factors of the continuous increase of nitrogen and phosphorus concentrations in Xingyun Lake were studied, and the advantages of the machine learning KNN algorithm in the prediction of nitrogen and phosphorus contents in lake surface sediments were discussed. The results showed that the TN content in the surface sediments of Xingyun Lake ranged from 0.56% to 0.86%, with an average of 0.71%, and the TP content was between 0.57% and 0.91%, with an average of 0.78%.The spatial distribution of nitrogen and phosphorus predicted by the four algorithm models had a certain spatial similarity, but even under the conditions of different periods, the spatial interpolation prediction error of the KNN algorithm was the smallest, and the fitting accuracy was higher than that of the traditional interpolation model. It was found that the spatial prediction accuracy of the KNN model was higher when the concentration of nitrogen and phosphorus was lower.The results showed that the concentrations of nitrogen and phosphorus in the surface sediments of Xingyun Lake showed an overall upward trend, and the differences in different periods and spaces were mainly affected by land use types, agricultural non-point sources and natural factors of the lake.The research results will provide some reference for the spatial prediction of nutrients in surface sediments of low-latitude plateau lakes and the ecological protection of lakes. Related Articles | Metrics

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Screening of antimicrobial and anti-quorum sensing activities of marine actinomycetes isolated from mangrove sediment in Hainan MO Jie, QIAN Shenghui, QIAN Jiaxing, MIAO Li 2024, 41 (1):  88.  doi: 10.3969/j.issn.2095-1736.2024.01.088 Abstract ( 38 )   PDF   Save The antibacterial and anti-quorum sensing activities of 84 actinomycetes isolated from mangrove sediment against the indicator bacteria, Chromobactierium violaceum, Escherichia coli and Staphylococcus aureus, were tested by disc diffusion assay. The highly active strains were selected for secondary activity screen and chemical diversity analysis by thin layer chromatography. The results showed that strain HN2-56 had strong anti-quorum sensing activity and weak antibacterial activity, and its metabolites were abundant. The secondary metabolites of this strain were preliminary isolated and analyzed by GC-MS. 13 possible compounds were identified and many of them had certain biological activity. The activity verification results showed that compounds 4 (diethyl toluamide) and 12 (curcumenol) had anti-quorum sensing activity. Through 16S rDNA sequence analysis, morphology observation and phylogenetic analysis, the strain HN2-56 was identified as Streptomyces chumphonesis. This strain has certain application potential in anti-quorum sensing and has the value of further study. Related Articles | Metrics

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Isolation, identification and degradation characteristics of a chloramphenicol-degrading bacterium, Pseudoxanthomonas mexicana CC18 CHEN Tian, ZHOU Jia, WANG Yiping, GENG Ningyu, QU Jianhang, ZHAO Shuai, GU Xie 2024, 41 (1):  94.  doi: 10.3969/j.issn.2095-1736.2024.01.094 Abstract ( 40 )   PDF   Save In order to explore the microbial strain resources for chloramphenicol degradation, a chloramphenicol-degrading strain CC18 was isolated and screened from the activated sludge in the biological contact oxidation reactor treating chloramphenicol wastewater. The CC18 was identified as Pseudoxanthomonas mexicana based on its morphological, physiological and biochemical characteristics, and 16S rRNA gene sequence analysis. The optimal conditions for degradation chloramphenicol by strain CC18 were 4% inoculum, pH 7, and at incubation temperature 28 ℃. The degradation efficiency of 20 mg/L chloramphenicol after 24 h was 22.9%. The response surface results further suggested that the degradation efficiency of 20 mg/L chloramphenicol by strain CC18 was 30.6% after 24 h when the inoculum was 3.6%, pH was 7.6, and the temperature was 30 ℃. The ability of Pseudoxanthomonas mexicana to degrade chloramphenicol was found for the first time in this study, which not only enriched the chloramphenicol degrading bacterial resource, but also provided a theoretical basis for the microbial enhancement treatment of chloramphenicol contamination in the environment. Related Articles | Metrics

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Mechanism and research advances of hydrogen production by green algae #br# 2024, 41 (1):  100.  doi: 10.3969/j.issn.2095-1736.2023.06.100 Abstract ( 61 )   PDF   Save This review systematically introduced three different pathways of hydrogen production by hydrogenase in green algae under anaerobic condition, including direct hydrogen production via photosystem II, indirect hydrogen production independent of photosystem II and hydrogen production by dark fermentation. The advantages and disadvantages of each pathway were analyzed. However, limited by technology and high costs, hydrogen production by green algae was still in the laboratory-scale, and will take a long time to achieve industrial production. Aiming at the efficient hydrogen production, this review described the latest outcome in this field and summarized the methods for optimizing the hydrogen production, including hydrogenase modification, reducing the oxygen content, lowering the cyclic electron transfer rate, reducing the Calvin-cycle, remodeling the light harvesting complex, pretreatment of the biomass and photo-bioreactor design. The future development of hydrogen production by green algae was also discussed. Related Articles | Metrics

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Research progress on extracellular electron transfer in electricigens based on network and omics methods #br# DING Dewu, ZHANG Kun, HE Xiaoqing, XIE Jianming 2024, 41 (1):  107.  doi: 10.3969/j.issn.2095-1736.2024.01.107 Abstract ( 39 )   PDF   Save The background and the significance of the research on extracellular electron transfer (EET) in electricigens were introduced. The roles of various molecules such as cytochrome c, pili proteins and electron mediators involved in the EET process were summarized, and the relevant works on the use of network methods and omics methods to study the EET process of electricigens in recent years were reviewed. The applications of network analysis methods in the identification of key electron transfer molecules, mining of electron transfer modules and inference of electron transfer pathways were summarized from several aspects, such as protein networks, regulatory networks and integrated networks; the researches on the use of omics methods to identify electron transfer genes and their functional analysis were summarized from several aspects, such as genome, transcriptome, proteome, multi-omics and meta-omics; and the integration of biological networks and omics data to study the coordinated utilization of biomolecules, identification of key genes and gene clusters in the EET process of electricigens were also introduced. The current problems were discussed, and the future research directions of integrating multiple biological networks and multi-omics data to carry out EET research on electricigens were prospected. Related Articles | Metrics

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Study on immobilization of sucrase by nano-flower CUI Shiqi, JIANG Xiaodong, WANG Shijie, WANG Hongying, QIAN Siriguleng 2024, 41 (1):  113.  doi: 10.3969/j.issn.2095-1736.2024.01.113 Abstract ( 54 )   PDF   Save Sucrase (Sucrase, EC 3.2.1.26) has high economic value in industry, and can be used to decompose sucrose to produce fructose and glucose. Nowadays, there are problems such as single sucrase fructose production method, not easy to maintain vitality and difficult to reuse. The nano-flower immobilized enzyme of Mn3(PO4)2/sucrase \[hereinafter referred to as Sucrase@Mn3(PO4)2\] was prepared by co-precipitation method, and the preparation conditions were optimized. The morphology and structure of the immobilized enzyme were characterized and the kinetic characteristics of the immobilized enzyme were analyzed. The results showed that the nano-flower particles of Sucrase@Mn3(PO4)2were uniform in size, dispersed, daisy-shaped and blooming. The internal crystal structure of nano-flower immobilized enzyme was complete. The immobilized enzyme had obvious EDS characteristic peaks of Mn2+, PO3-4and enzyme protein, and had obvious FT-IR absorption characteristics of enzyme protein and phosphoric acid. It showed good temperature stability and acid-base stability, and maintained its original activity of 40.38% after repeated use for 7 times and 40% when stored at 4 ℃ for 28 days. Related Articles | Metrics

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Nobel Prize cases-based teaching practice in “Molecules and cells”,a fundamental course of the speciality of biomedical engineering JIANG Hui, LI Yan 2024, 41 (1):  120.  doi: 10.3969/j.issn.2095-1736.2023.06.120 Abstract ( 65 )   PDF   Save In recent years, we have explored the pilot reform of Nobel Prize cases as a main clue to guide the course of “Molecules and Cells”. We selected 15 award cases covering 11 core chapters of the course, each of which was jointly investigated by two students. The students are recommended to use the ceremony report of Nobel Prize winner as a blueprint, focusing on the research background, the highlights of discoveries/inventions, and the theoretical or applied values by the scientists. In the in-class practice, a standard unit of introduction-report-discussion-summary is constructed, using the method of panel discussion to improve teaching effectiveness. The strategy integrates our national needs, science and art, facing challenges, and innovative spirit. This plan has stimulated students’ enthusiasm for self-directed learning, cultivated students’ basic biomedical qualities and concepts through extracurricular research and in-class discussions. This reform has achieved beneficial performances under limited course time and 90% students have achieved significant results after course learning. Through teaching practice, we have further proposed improvement ideas such as method optimization, viewpoint update, ideological and political integration, and perspective enhancement, which has potential reference significance for the teaching reform of life foundation courses in biomedical majors. Related Articles | Metrics

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A new method to extract DNA from Drosophila melanogaster suitable for experimental teaching #br# WEI Yuan, WANG Honggang, ZHU Yushan, LI Yanjun 2024, 41 (1):  123.  doi: 10.3969/j.issn.2095-1736.2024.01.123 Abstract ( 69 )   PDF   Save The common animal tissue DNA extraction method has been modified in the genetics course at Nankai University. This novel method of extracting DNA from Drosophila melanogaster can be applied in scientific research as well as in experimental teaching scenarios. The method comprises three steps which are namely alkali cracking, acid neutralization, and centrifugation. The method is characteristically simple, quick, stable, and cost-effective. The concentration and purity of the extracted DNA enable subsequent PCR amplification and are also conducive to experimental teaching. This method has simplified the process of extracting DNA from Drosophila melanogaster and has laid a foundation for the improvement of experimental projects related to Drosophila melanogaster gene expression analysis. In addition, it has helped to improve the experimental teaching effect and curriculum design. Related Articles | Metrics

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Reform and innovation of the course genetic engineering under the background of new agricultural science FANG Zhongming 2024, 41 (1):  126.  doi: 10.3969/j.issn.2095-1736.2024.01.126 Abstract ( 70 )   PDF   Save The construction of the “new agricultural science” requires the reform of the links and methods of cultivating agricultural and forestry talents, and the cultivation of innovative, composite, and practical new agricultural talents. Based on this, our university has carried out course reform and innovation from three aspects: editing and publishing the textbook “genetic engineering”, and creating a teaching system of genetic engineering with production application as the main line; and reforming the course syllabus, lesson plans and courseware using the BOPPPS method for teaching design; organizing and participating in disciplinary competitions to promote teaching and learning through competition. The organic connection of the three teaching links before class, during class, and after class was achieved, the rationality of the teaching system, the feasibility of teaching design, and the effectiveness of teaching guidance were tested, the comprehensive qualities and innovative abilities of students were effectively cultivated, and the construction of the national first-class undergraduate major “agricultural science” was promoted through the “three step method” of innovation, reform, and competition mentioned above. It could provide reference for the cultivation of innovative and high-quality agricultural talents in the new era. Related Articles | Metrics