Abstract: C2H2 zinc finger protein, as one of the largest transcription factor family in plants, plays an important role in plant growth and stress. In this study, the zinc finger protein TaZFP33 gene in the spring wheat variety Fielder（Triticum aestivum L. cv. Fielder） was cloned. The TaZFP33 gene coding region was 510 bp in length and encoded 169 amino acids. Bioinformatics analysis showed that the molecular weight of TaZFP33 protein was 17.5 ku, and the isoelectric point was 6.43, which belonged to the Q-type zinc finger protein and was an unstable hydrophobic protein. Evolutionary analysis and promoter analysis indicated that the gene may respond to ABA-induced stress regulation. Transient expression analysis of transformed tobacco showed that TaZFP33 was localized on the nucleus. Real-time fluorescence quantitative analysis of tissue-specific expression showed that TaZFP33 gene was expressed in stems, leaves, roots and embryos, and the highest expression level was in embryos. After seedlings were treated with different concentrations of ABA, PEG6000 and NaCl, the results showed that TaZFP33 gene responded to above-mentioned stresses. This research laid a foundation for further exploration on the biological function of the TaZFP33 gene, and also provided candidate genes for the study of stress resistance in Triticum aestivum.

Key words: Triticum aestivum, TaZFP33, gene cloning, bioinformatics, subcellular localization, expression analysis