Abstract: In order to make up for the disadvantages of traditional microbiology experiment assessment, verification and comprehensive experiments were no longer set up alone, just one large comprehensive experiment was designed with the condition of limited teaching hours, which was disintegrated into eight small experimental items with verification, synthesis and design. Under the “3+1” experimental teaching mode, each small experimental item was quantitatively scored according to the four steps of experimental preparation, intelligent guidance, experimental operation and summary innovation, the final experiment examination was no longer arranged, and the experimental scores of students were comprehensively determined according to the scores of each step of each small experiment item done in the “3+1” teaching mode. The degrees of the students’ learning and understanding for each small experimental item could be judged based on the analysis of each highscore segment score of each small experimental item. Compared with the traditional microbiology experiment teaching examining method, the examination of microbiology experiment under the mode of “3+1” teaching reform paid more attention to the whole process of each small experimental item to make a quantification, the examination standard was clear, the examination content was comprehensive as well as the offline assessment of the examination mode was combined with online, which reduced the probability of score teachers assess randomly; the probability of speculative score of students and effectively promoted the students to participate in the whole teaching of the experiment, and their practical ability and ability of innovative summary were both improved. It also enabled teachers to acquainte objectively with the effect of experimental teaching and learning of students, and find out the problems existing in experimental teaching exactly and improve them.

Key words: “3+1” teaching mode, microbiology experiment, experiment examining of per item, quantitative assessment of the whole process