Abstract: Pharmaceutical microbiology is an important applied branch of microbiology and it provides broad knowledge and understanding with regards to principles, techniques and processes involved in pharmaceutical microbiological experiments.Integrated project-based learning (PBL) methodology into the preparation before class, classroom explanation, experiment process and experiment evaluation of pharmaceutical microbiology laboratory course, a new experimental and practical teaching reform was conducted. This PBL experiment guided students to experience an entire inquiry-based learning process by consistently conducting research projects, through which students would gain an in-depth knowledge of the fundamentals of pharmaceutical microbial experiment skills.The PBL experiment helped student’s gain thorough understanding and reveal profound insights into pharmaceutical microbiology. It essentially provided an ample emphasis on the vivid coverage of the procedures involved in isolation of antagonistic microorganism and improved student’s interest of study, the operational skills and scientific innovative abilities and also enhanced the quality of pharmaceutical microbiology education and teaching.

Key words: harmaceutical microbiology, project-based learning methodology, research project；experiments, teaching reform