Abstract: To explore the cytological function of extracellular vesicles fromLactobacillus plantarum, extracellular vesicles fromL. plantarum（LPEV） suspension were extracted by differential centrifugation, the vesicles morphologic features were observed with a transmission electron microscope; the concentration was detected by nanoparticle tracking analysis （NTA）; and the vesicles cytological function was verified by cell proliferation, cell scratch, Transwell, qRT-PCR and western blot. The results showed that the extracellular vesicles with the concentration about 2.3×1010 grain number/mL isolated fromL. plantarumwere indeed vesicle-like under electron microscope; the results of cell proliferation, cell scratch and Transwell showed that the vesicles could promote cell proliferation and enhance cell activity; qRT-PCR and western blot results showed that the vesicles had up-regulation effects on genes and proteins related to skin regeneration. The comprehensive experimental results confirmed that the vesicles had certain potential in regenerative medicine, which laid experimental foundation for further investigation of specific regulatory mechanisms.

Key words: Lactobacillus plantarum, extracellular vesicles, extraction, identification, cytothesis