Abstract: In this study, a strain of rubber anthracnose isolated and preserved in our laboratory was identified for antagonistic agents to provide a theoretical basis for the control of rubber anthracnose. The strain was identified by a combination of molecular biology and morphology, its biological characteristics were studied, and the inhibition effect of biological and chemical agents on the strain was compared. The results showed that this strain was identified as Colletotrichum fructicola and was named HD-1. The optimum medium, carbon, and nitrogen sources for growth were potato dextrose agar medium (PDA), sorbitol, and beef extract, with pH of 8.0. Through testing and comparison, it was found that Prochloraz, Difenoconazole, and Bacillus velezensis HN-2 n-butanol extracts had an obvious inhibitory effect on strain HD-1. Only the mycelium treated with HN-2 n-butanol extract was found to be expanded by microscopic observation. This experiment result would provide the theoretical basis and technical support for the prevention and control of rubber tree anthracnose in the field.

Key words: anthracnose, identification, Colletotrichum fructicola, biological characteristics, EC₅0