Abstract: To research environment-friendly extraction method, ultra-high pressure method was used to destroy bone structure in nature environment and to accelerate the release of bovine protein. The effects of time, pressure and pH on the extraction rate of protein from bovine bone meal were studied by single factor and response surface optimization experiment. The optimal conditions for extraction of bovine bone protein under ultra-high pressure were obtained. The regression model of response surface experiment was significant and the error was small. The model was suitable for theoretical interpretation of this experiment. The results showed that the protein extraction rate reached 35.62% when time was 54 min, pressure was 189 MPa and pH was 6.7. When the number of extraction was increased to three times, the extraction rate reached 54.2%. And it could reach 59.3% when hydrolyzed with enzyme. It was established an ultra high pressure the environment-friendly bovine bone protein extraction method.

Key words: bovine bone protein, ultra-high pressure extraction, response surface optimization, environment-friendly extraction, process optimization