Abstract: Recombinant spider silk, as a kind of biological material with good performance, owns a great biocompatibility, meanwhile, there is great potential application value in the field of biomedical. Spider silk has potential application value in blood vessels, nerve conduit and drug carriers, but still lacks research in the tissue engineering. In this study, spidroin PySp was expressed in E.coli as the material. By investigating the electrospinning conditions, fiber membrane was obtained at a suitable spinning voltage and other conditions. SEM displayed that fiber membrane is uniform. The ATR-FTIR graph showed that the secondary structure of the electrospun fibers cross-linked by 75% ethanol is mainly β-folded. The contact angle revealed that the fiber membranes are hydrophilic. This study has provided a type of good fiber material for the application of spider silk protein in tissue engineering and laid a foundation for the further application of spider silk.

Key words: spidroin, electrospinning, fiber membrane, performance characterization