Journal of Biology ›› 2023, Vol. 40 ›› Issue (1): 69-.doi: 10.3969/j.issn.2095-1736.2023.01.069

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Genetic diversity analysis of Corbicula fluminea in the Tai Lake based on mitochondrial COI and Cytb gene sequences

LI Daming1, LIU Yang1,2, LIU Yanshan1, TANG Shengkai1, GU Xiankun1, YIN Jiawen1,JIANG Qicheng1, ZHANG Tongqing1   

  1. 1. Freshwater Fisheries Research Institute of Jiangsu Province, Key Laboratory of Fisheries Resources and
    Environment in Inland Water, Nanjing 210017, China;
    2. College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China
  • Online:2023-02-18 Published:2023-02-21

Abstract: The mitochondrial COI and Cytb partial gene sequences were used as molecular markers to analyze and evaluate the genetic diversity of Corbicula fluminea in the Tai Lake. The 614 bp-COI and 621 bp-Cytb gene sequences were obtained from 40 individuals of C. fluminea by PCR amplification and sequencing. Meanwhile, the content of A+T was much higher than that of G+C in both COI and Cytb sequences indicated that there was obvious bias for base composition. 19 variable sites were detected and 14 haplotypes were defined in COI sequences, the average haplotype diversity and nucleotide diversity were 0.851±0.04 and 0.00745±0.00081, respectively, the average number of nucleotide differences was 4.572, and the genetic distance among haplotypes ranged from 0.002 to 0.020. 19 variable sites were detected and 17 haplotypes were defined in Cytb sequences, the average haplotype diversity and nucleotide diversity were 0.896±0.028 and 0.00510±0.00051, respectively, the average number of nucleotide differences was 3.167, and the genetic distance among haplotypes ranged from 0.002 to 0.013. Therefore, the genetic diversity of C. fluminea showed high haplotype diversity and high nucleotide diversity pattern. The Tajima’s D and Fu’s Fs values of C. fluminea were negative but no significantly different, and mismatch distribution analysis showed a multiple type, indicating that C. flumineain the Tai Lake had remained relatively stable and had not experienced population expansion in the history. The present results provided scientific information for conservation and sustainable utilization of C. fluminea germplasm resources in Tai Lake.

Key words: Corbicula fluminea, genetic diversity, COI gene, Cytb gene, population history

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