Abstract: From October 2019 to October 2021, 2551 insect pollinators were collected in the typical forest-agriculture ecotone of Yaoluoping Nature Reserve. 92 species of pollinators were identified by morphology and DNA Barcoding, belonging to 73 genera, 34 families and 4 orders, among which Apis cerana, Bombus trifasciatus, Eristalis cerealis and Pieris canidia were the dominant species. The results of species diversity analysis showed that transect I dominated by farmland had higher species diversity than transects II and Ⅲ dominated by forests, because a variety of crops can provide better habitat and food source for pollinators, while transect Ⅲ, which was near a tourist area severely disturbed by human, had significantly lower diversity. In addition, 19(Bio1-Bio19) environment factors were downloaded from WorldClim database to analyze the correlation between diversity (including species and phylogenetic diversity) and environmental factors. There was a linear correlation between 14 environmental factors (Bio1, Bio4-13, Bio16, Bio18-19) and diversity index (including phylogenetic diversity and species richness). The results of redundancy analysis supported that temperature factors had a more significant effect on phylogenetic diversity and richness. Temperature could be the key ecological factors influencing the diversity of pollinators in the reserve.

Key words: pollinator, species richness, DNA barcoding, phylogenetic diversity, environmental factor