Table of Content

18 August 2023, Volume 40 Issue 4 Previous Issue    Next Issue

Select

Effects of yellow mealworm (Tenebrio molitor) meal replacing soybean meal on the hepatic and intestinal health of Nile tilapia (Oreochromis niloticus) and mechanism exploration#br# ZHANG Le, SUN Qi, LI Weijie, WU Hongxia, QIAO Fang, DU Zhenyu, CHEN Liqiao, ZHANG Meiling 2023, 40 (4):  1.  doi: 10.3969/j.issn.2095-1736.2023.04.001 Abstract ( 414 )   PDF   Save To evaluate the effects of yellow mealworm meal (YM) as a partial replacement for soybean meal (SBM) on the hepatic and intestinal health of fish, four groups of isonitrogenous (320 g/kg crude protein) and isoenergetic (16.8 MJ/kg) experimental diets were formulated using YM to replace 0, 15%, 30%, and 45% SBM in an SBM-based diet for Nile tilapia (Oreochromis niloticus). A ten-week feeding experiment was conducted and the parameters related to the hepatic and intestinal health were analyzed. The results indicated that YM could replace 15% SBM in tilapia feed without negative effects on the hepatic and intestinal health. However, when the replacement ratio was up to 30%, decreased intestinal muscular thickness, shorter intestinal villi, lower intestinal transepithelial electrical resistance and higher mRNA expression level of tight junction protein-related genes were observed. When the replacement ratio was up to 45%, the intestinal injury was further exacerbated, and hepatic injury occurred. Hepatic injury was manifested by a significant increase in serum alanine transaminase activity, blurred hepatocyte boundaries, lipid droplet accumulation, and a significant increase in total lipids, triglycerides, non-esterified free fatty acids, and glycogen content. Transcriptome analysis revealed that genes related to glycogen synthesis and apoptosis were significantly enhanced in liver in YM45. Further analysis revealed thatklf9, an oxidative stress-related gene, was significantly upregulated in both liver and intestine, suggesting that oxidative stress occurred in YM45 group. Related Articles | Metrics

Select

Function analysis of leucine 94 in light-driven proton pump archaerhodopsin-4 LIU Dongxue, ZHAO Xin 2023, 40 (4):  11.  doi: 10.3969/j.issn.2095-1736.2023.04.011 Abstract ( 206 )   PDF   Save Site-directed mutation technique was used to mutate L93/L94 (leucine 93/leucine 94) in bR/aR4 (bacteriorhodopsin/archearhodopsin-4) to alanine and phenylalanine with different side chains, and threonine with different polarities, respectively. The corresponding bR, BI-aR4 (bacterioruberin-included), and BE-aR4 (bacterioruberin-excluded) mutants were obtained. UV-visible absorption spectroscopy, flash photolysis spectroscopy, and pH titration measurements were used to study the effects of L94 on the retinal, photocycle, proton pumping, and the relation between L94 and bacterioruberin. The results showed that L94 regulated the conformation and dynamic equilibrium of the retinal and the helical distribution of the proton channel through the steric hindrance in aR4 to maintain the retinal cis-trans isomerization and re-isomerization and promote proton release. In addition, L94 seemed to be modulated by bacterioruberin. The L94 mutants perturb helixes more strongly in BE-aR4, resulting in changing the hydrogen bond network of the proton donor and proton release cluster. Related Articles | Metrics

Select

Enhancement of phenazine-1-carboxylic acid production for Pseudomonas chlororaphis GP72-ND3ΔphzO by fermentation optimization LIU Zhenzhen, WANG Wei, HUANG Xianqing, ZHANG Xuehong 2023, 40 (4):  20.  doi: 10.3969/j.issn.2095-1736.2023.04.020 Abstract ( 228 )   PDF   Save The fermentation conditions of this strain were optimized and scaled up in a 5 L bioreactor. First, through single factor experiment, orthogonal experiment, steepest climbing experiment and central composite design experiment at the shake flask level, the optimal medium composition was obtained as 49.55 g/L glycerol, 37.34 g/L soy peptone, 1.5 g/L KCl, 0.75 g/L MgSO4and 0.225 g/L K2HPO4, and the maximum production of phenazine-1-carboxylic acid (PCA) after 60 h fermentation was （6.01±0.17） g/L, which was 2.6 times as that before optimization. Then, the fermentation pH was optimized in 1 L bioreactors. When the pH was maintained at 6.8 during the culture process, the PCA production was the highest at （7.16±0.04） g/L, which was 1.2 times as that before optimization. Finally, under the optimal medium and pH conditions, the strain was scaled up cultured in 5 L bioreactors, and the maximum PCA production after 60 h of fermentation was (6.84±0.53) g/L. This study provided support for improving the production and industrial application of PCA. Related Articles | Metrics

Select

Antibacterial activity of tap zinc phthalocyanine photosensitizer against Escherichia coli YAN Shufeng, LIAO Xiaoyun, SHI Qi, WU Hanqin, HUANG Xiaochen 2023, 40 (4):  27.  doi: 10.3969/j.issn.2095-1736.2023.04.027 Abstract ( 197 )   PDF   Save To study the antibacterial activity and mechanism of tap (2,4,6-tris (Dimethylaminomethyl) phenol) zinc phthalocyanine photosensitizer against Escherichia coli (E.coli, ATCC 25922), the growth curve of E.coli was determined by UV, and the antibacterial activity of TAP zinc phthalocyanine photosensitizer on E.coli was studied by agar plate method. The metabolic activity was measured via the CCK-8 assay. The mode of action of tap zinc phthalocyanine photosensitizer was analyzed by ROS probe method (Quantifying ROS levels using DCFH-DA probe). Results showed that the logarithmic growth period of E.coli was about 4-10 h after inoculation. TAP zinc phthalocyanine photosensitizer had significant photodynamic antibacterial activity against E.coli, and its antibacterial effect was directly proportional to the concentration of photosensitizer. At the same time, it was proved that TAP zinc phthalocyanine photosensitizer played an antibacterial role by producing hydroxyl free radical singlet oxygen. This research proved that TAP zinc phthalocyanine photosensitizer had strong photodynamic antibacterial effect on E.coli, which provided evidence for the development-series of antibacterial products based on TAP zinc phthalocyanine photosensitize for antibacterial purpose. Related Articles | Metrics

Select

Inhibition and preliminary mechanisms of a FoxM1-targeting polypeptide 9R-P49 on fibroblast L929 cells CHANG Miao, XIANG Kun, HE Jiameng, LIANG Anping, HUA Xinyi, LIU Xinrong, JIANG Yuhong, MAO Canquan 2023, 40 (4):  31.  doi: 10.3969/j.issn.2095-1736.2023.04.031 Abstract ( 212 )   PDF   Save To explore the regulatory effect of 9R-P49 peptide on FoxM1 and its preliminary mechanism on fibroblast L929 cells, CCK-8 was used to detect cell inhibition rate, AO/EB double staining and flow cytometry to detect cell apoptosis, transwell assay to detect cell migration and cell plate clone to detect cell proliferation. The potential binding sites of the P49 peptide to FoxM1-DNA binding domain（FoxM1-DBD） were predicted using PyMOL molecular docking software. Mouse fibroblast L929 cells were treated with 0, 30.0 and 60.0 μg/mL 9R-P49 peptide and the expression of FoxM1 protein was detected by Western Blot. Finally, the differential expressed genes and related pathways were analyzed by RNA transcriptome. The results showed that 9R-P49 could inhibit FoxM1 protein expression in L929 cells. At the same time, 9R-P49 could inhibit cell proliferation and promote cell apoptosis of L929 cells. P49 peptide binds potentially to the Arg 297, Ser 290 and Asp 293 sites on FoxM1-DBD. The GO classification of differentially expressed genes in the transcriptome after drug treatment was mainly enriched in cellular process, single-organism process, biological regulation, and metabolic process. The KEGG classification was mainly enriched in the immune system, endocrine system, signal transduction, and metabolism pathways. In conclusion, 9R-P49 can inhibit the proliferation of L929 cells and promote apoptosis by regulating the expression of FoxM1 in L929 cells. Related Articles | Metrics

Select

Identification of SARS-CoV-2 based on RNA barcoding JIANG Shuai, YOU Changqiao, ZHANG Hongming, QIN Hong, GUO Xinhong 2023, 40 (4):  37.  doi: 10.3969/j.issn.2095-1736.2023.04.037 Abstract ( 184 )   PDF   Save It aimed to design a technology for the faster and more accurate identification of SARS-CoV-2 through RNA barcode segments. Sequences of Beta-CoV genera and 7 HCoVs were screened to construct the sequences database based on the NCBI online database. The genetic diversity analysis, genetic distance analysis and phylogenetic tree construction of the sequences database were performed on bioinformatics methods, so as to examine the accuracy and stability of barcode segments in identifying SARS-CoV-2 in different cases. Finally, the fragments clipped from the SNP sites on sequences were scored by using the Blast on NCBI to obtain the barcode segments and visual barcodes with the optimal identification effect. Above results showed that the RNA barcode segments could precisely identify SARS-CoV-2 from all strains within the sequences database, and the two barcode segments (located in the coding regions of ORF1ab gene and S gene respectively) screened with Blast Total scores and P value tests also had good stability for identifying SARS-CoV-2. RNA barcoding contributed to explore the relationship between the whole genome sequence polymorphism of SARS-CoV-2 and the species-specific genetic markers and to provide new idea for identification technologies of SARS-CoV-2. Related Articles | Metrics

Select

The antioxidant capacity of natural astaxanthin against H2 O2 analyzed by fluorescence spectrometry ZHENG Xinxin , HUANG Qing 2023, 40 (4):  42.  doi: 10.3969/j.issn.2095-1736.2023.04.042 Abstract ( 273 )   PDF   Save In order to explore the antioxidant capacity of astaxanthin to H2O2, a new method for determining the oxidation capacity of astaxanthin to remove H2O2was established by detecting and analyzing the changes and effects of antioxidants on the fluorescence spectrum of biphenyl dimers under the catalytic conditions of horseradish peroxidase (HRP) using homovanillic acid (HVA) to produce a strong fluorescent signal. And the antioxidant effect of astaxanthin against H2O2was compared with that of β-carotene, vitamin C and α-tocopherols. The results showed that the H2O2clearance capacity of astaxanthin was significantly higher than that of β-carotene, vitamin C and α-tocopherol, which was about 1.4 times that of β-carotene, 8 times that of vitamin C, and 732 times that of α-tocopherol. The antioxidation capacity of natural astaxanthin against H2O2was analyzed by fluorescence spectrometry, which provides new evidence for the excellent antioxidant function of astaxanthin, and is also conducive to people’s understanding and exploration of the antioxidant function of astaxanthin. Related Articles | Metrics

Select

Phylogenetic relationship of Mesostigmata based on the mitochondrial 16S rRNA gene sequence YANG Huijuan, CHEN Ting, DONG Wenge 2023, 40 (4):  48.  doi: 10.3969/j.issn.2095-1736.2023.04.048 Abstract ( 221 )   PDF   Save To understand the complete sequence information of mitochondrial 16S rRNA gene and the phylogenetic relationships of Mesostigmata species, the mitochondrial genome of Parasitus fimetorum was sequenced by PCR and combined with the sequence information of most taxa of Mesostigmata in the NCBI database. A comparative analysis of mitochondrial 16S rRNA genes of Mesostigmata species was performed using bioinformatics software to further explore the phylogeny and relation among Mesostigmata species. The results showed that the AT content of the mitochondrial 16S rRNA genes of Mesostigmata species was much higher than the GC content; the genetic distance ranged from 0.111 to 0.359, with an average genetic distance of 0.291. The base conversion to inversion ratio and sequence saturation analysis showed that the mitochondrial 16S rRNA genes of Mesostigmata species had a high evolutionary potential suitable for phylogenetic analysis. The phylogenetic trees constructed using ML and BI methods showed that Parasitidae formed sister branches with the Ologamasidae, which differed from the traditional taxonomic results. Most species of the same family always preferentially clustered together, indicating that the mitochondrial 16S rRNA gene was used to construct phylogenetic relationships of Mesostigmata with stable and reliable characteristics. The above results provided a foundation for the subsequent study of the phylogenetic relationships of Mesostigmata species. Related Articles | Metrics

Select

Complete mitochondrial genome sequence and phylogenetic analysis of Rana kukunoris ZHANG Xuze, DONG Bao, HA Jinqiang, ZHAO Ying, WEI Shengnan, WEI Yingting 2023, 40 (4):  54.  doi: 10.3969/j.issn.2095-1736.2023.04.054 Abstract ( 241 )   PDF   Save The high-throughput sequencing of illumina platform was used to sequence the whole mitochondrial genome of Rana kukunoris. Then, the mitochondrial genome was assembled and annotated. The total length of the sequence was 21913 bp, which mainly included four parts: tRNA gene (22), rRNA gene (2), CDS (13) and D-loop (1). ExceptNAD6in the light chain, other protein coding genes were on the same chain. Of the coding genes, three overlapping genes were found:COX1/trnS2,ATP6/ATP8andNAD4L/NAD4. Mitochondrial genome of Rana kukunoris had AT preference. Leu and Ser were the most frequently used amino acids in protein coding genes, and all tRNA had the classic clover structure. KaKs analysis showed that the coding genes of mitochondrial genome were not significantly positively selected in Rana kukunoris. The phylogenetic tree constructed based on the whole mitochondrial genome showed that Rana kukunoris was closely related to Rana chensinensis. It provided the genetic information of Rana kukunoris mitochondrial genome and aid future investigation of phylogenetics, speciation and evolution of Rana genus. Related Articles | Metrics

Select

Morphology and growth characteristics of otoliths of two species of Sciaenidae in the marine ranching area of Haizhou Bay ZHANG Shuo, ZHANG Xiao, GAO Shike, SUN Wen 2023, 40 (4):  62.  doi: 10.3969/j.issn.2095-1736.2023.04.062 Abstract ( 219 )   PDF   Save The morphological parameters, growth characteristics of otoliths of Collichthys lucidus and Larimichthys polyactis and their relationship with fish growth were studied. The left sagittal otolith was selected as the research object, and the growth relation of each parameter of sagittal otolith was fitted by linear, logarithmic, power and exponential functions. The results showed the long and short diameters of the sagittal otoliths of two species increased with the increase of body length and weight, but the ratio of the long diameter to the short diameter was basically stable. There were significant power function correlations not only between the long and short diameters of sagittal otoliths and body length of C. lucidus, but also between the long and short diameters and body weight. There was a significant linear correlation between the long diameter and short diameter of sagittal otolith of small yellow croaker and body length and weight. For the growth characteristics of sagittal otolith, there were obvious differences between the two kinds of fish. The ratio of short diameter to long diameter of sagittal otolith of C. lucidus was stable at 0.75, while that of L. polyactis was 0.6. There was a significant power function correlation between the length and diameter of the sagittal otolith and the otolith weight of C. lucidus, while that of L. polyactis was a linear function correlation. The random forest results showed that the contribution rates of otolith morphological parameters to body length and weight of the two species were different, but the difference was not significant. Related Articles | Metrics

Select

Diversity of pollinators and their relationships with environmental factors in typical forest-agriculture ecotone of Yaoluoping Nature Reserve YAO Chenchen, HOU Yinxu, GUO Dongxu, WAN Xia 2023, 40 (4):  70.  doi: 10.3969/j.issn.2095-1736.2023.04.070 Abstract ( 202 )   PDF   Save From October 2019 to October 2021, 2551 insect pollinators were collected in the typical forest-agriculture ecotone of Yaoluoping Nature Reserve. 92 species of pollinators were identified by morphology and DNA Barcoding, belonging to 73 genera, 34 families and 4 orders, among which Apis cerana, Bombus trifasciatus, Eristalis cerealis and Pieris canidia were the dominant species. The results of species diversity analysis showed that transect I dominated by farmland had higher species diversity than transects II and Ⅲ dominated by forests, because a variety of crops can provide better habitat and food source for pollinators, while transect Ⅲ, which was near a tourist area severely disturbed by human, had significantly lower diversity. In addition, 19(Bio1-Bio19) environment factors were downloaded from WorldClim database to analyze the correlation between diversity (including species and phylogenetic diversity) and environmental factors. There was a linear correlation between 14 environmental factors (Bio1, Bio4-13, Bio16, Bio18-19) and diversity index (including phylogenetic diversity and species richness). The results of redundancy analysis supported that temperature factors had a more significant effect on phylogenetic diversity and richness. Temperature could be the key ecological factors influencing the diversity of pollinators in the reserve. Related Articles | Metrics

Select

Study on selection of special varieties in cold and humid areas in potato staple food research and industry development in China LI Yutao, TANG Dejing, ZHU Yongyong, XIE Kuizhong 2023, 40 (4):  76.  doi: 10.3969/j.issn.2095-1736.2023.04.076 Abstract ( 167 )   PDF   Save In order to select special potato varieties suitable for planting in cold and humid areas and promote the further implementation of potato staple food research and industry development in China, a total of 25 varieties were introduced and collected to carry out variety comparison test in cold and humid areas in China. The ecological adaptability (agronomic characters), processing quality indexes (dry matter, reducing sugar and starch content) and processing related enzyme activities (PPO and POD activities) were compared. By means of membership function, cluster analysis and correlation comprehensive analysis, 5 varieties suitable for local planting were screened out, which were Longshu 15, Longshu 14, Qingshu 9, Longshu 10 and Longshu 7. The main indexes of the recommended varieties were yield with 27941.83-31143.77 kg/hm2, 74.13%-76.51% water content, 0.08%-0.20% reducing sugar content, 17.59%-23.58% starch content, 6.44-10.82 U PPO activity. It was recommended that these varieties could be popularized and planted in cold and humid areas. Related Articles | Metrics

Select

Comprehensive evaluation of the effect of different disinfectants on the surface disinfection of quinoa seeds and their germination ZHU Lili, ZHANG Yemeng, LI Wancai, ZHAO Yali, CHEN Zhiguo 2023, 40 (4):  83.  doi: 10.3969/j.issn.2095-1736.2023.04.083 Abstract ( 249 )   PDF   Save To clarify the effect of seed surface disinfectants on quinoa, using Qingli No.11 and Qingli No.12 quinoa grains as experimental materials, five commonly used disinfectants (KMnO4, H2O2, NaClO , HgCl2and 75% alcohol) were selected to prepare different concentrations (0.1%, 0.5%, 1%, 1.5%, 2 %, 2.5% and 3%) and set different disinfection time (1, 5, 10, 15, 20, 25 and 30 min). L49 (41×72) orthogonal experiment was used to investigate the seed germination, seedling growth, embryo rupture rate, abnormal germination rate and moldy rate. The disinfection effect of different disinfectants treatment methods on quinoa seeds was comprehensively analyzed. The results showed that the types of disinfectants had obvious effects on the disinfection effect of quinoa seeds. Among them, KMnO4and NaClO had better disinfection effects. The quinoa seeds treated with HgCl2had the least pollution rate, but it had a greater toxic effect on seeds and seedlings, causing most seeds to break and germinate abnormally, and should not be used. H2O2treatment has less damage to the seeds, but the pollution rate is high, and the ideal disinfection effect cannot be achieved. According to the screening results of membership function method, it was concluded that 2.5% KMnO4treatment for 15 min was the best disinfection method for quinoa seeds. Related Articles | Metrics

Select

Two new record plants in Anhui Province#br# FU Qingxiang, QU Lili, ZHU Wanhui, CHEN Zijie, HE Yuting, ZHAI Wei, XIANG Xiaoyan, WU Ganlin 2023, 40 (4):  90.  doi: 10.3969/j.issn.2095-1736.2023.04.090 Abstract ( 191 )   PDF   Save This paper reported two new records of plant distribution in Anhui Province, namely Cyperus odoratus and Tripterospermum discoideum. The morphological characteristics of the two plants were described in detail. The discovery of these new distributions enriches the plant species resources in Anhui Province and was of great significance to the study of plant species diversity and richness in Anhui Province. The certificate specimens were deposited in the herbarium of the college of life sciences, Anqing normal university. Related Articles | Metrics

Select

Application of atmospheric and room temperature plasma mutagenesis in microbial and edible fungi mutation breeding LU Huan, SHEN Ling, SHANG Xiaodong, LIU Jianyu, WANG Ruijuan, YANG Hui 2023, 40 (4):  92.  doi: 10.3969/j.issn.2095-1736.2023.04.092 Abstract ( 555 )   PDF   Save The concept, mutagenesis mechanism and factors affecting the mutagenesis effect of atmospheric and room temperature plasma (ARTP) mutagenesis were mainly described. The application of ARTP mutagenesis technology in improving microbial strains such as bacteria, actinomycetes and yeast and improving their biosynthesis ability, as well as in breeding new strains of edible fungi, was introduced in this paper with the main contents of mutagenesis breeding of microorganisms and edible fungi as the main contents. The advantages and challenges of ARTP mutagenesis technology in the breeding of microorganisms and edible fungi were analyzed. It also showed that the research focuses in the future were to improve the technical level of rapid screening of target strains combined with high-throughput sequencing, transcriptome and proteomics, to deeply explore the genetic law of microorganisms and edible fungi mutated by ARTP and the mechanism of regulating the synthesis of bioactive substances. It was hoped that the result would provide new ideas for the research of edible fungi and microbial breeding through the establishment and application of new technologies, and then promote the germplasm innovation and industrial sustainable development of microorganisms and edible fungi. Related Articles | Metrics

Select

The role of Dof transcription factors in crop stress response and agronomic trait improvement #br# WANG Zemin, JIN Xin, ZHANG Feiyan, SI Huaijun 2023, 40 (4):  98.  doi: 10.3969/j.issn.2095-1736.2023.04.098 Abstract ( 291 )   PDF   Save The molecular mechanisms of DNA-binding with one finger (Dof) transcription factors in crop response to stress such as extreme temperature, drought and salt stress, and their important roles in agronomic trait improvement were analyzed. Combined with the effects of different abiotic stresses on plant growth and development, the signal sensing mode and signal transduction pathway of Dof transcription factors in crop response to stress were introduced. The regulation mechanism of Dof transcription factors and the action mode of stress tolerance and important secondary metabolites (RFOs, etc.) during the regulation of gene expression were analyzed. Moreover, from the perspective of multi-signal network regulation mechanism and the demand of global climate change for sustainable and high-quality crop production, the prospects and directions of further research and utilization of Dof transcription factors in crop bio-breeding were prospected. Related Articles | Metrics

Select

Development of rAAV5 anion exchange chromatography process XU Lin, LIANG Huanhuan, LI Hongying, HAN Xu, DAI Liubing, MA Chao, ZHU Tao 2023, 40 (4):  107.  doi: 10.3969/j.issn.2095-1736.2023.04.107 Abstract ( 272 )   PDF   Save A purification method was established for effectively increasing the proportion between vector capsids of rAAV5-a viral product containing the therapeutic DNA sequence and empty capsids, viral vectors lacking the therapeutic gene. For the rAAV5 samples obtained from affinity chromatography, several anion exchange chromatography and related gradient elution methods were tested, in which CIMmultus QA (CIMQ) can effectively remove empty capsids from vector capsids. For the CIMQ purification, the buffer matrix and the collection of rAAV5 fractions were optimized. Subsequently, qPCR was used to quantify the recovery rate of the virus genome (Vg) content, and high-performance liquid chromatography (HPLC) or size exclusion chromatography-multi-angle static light scattering (SEC-MALS) was used to determine the vector capsids proportion of rAAV5 samples. In the CIMQ purification process, the proportion of vector capsids in the collected fraction (at UV absorbance UV260/UV280 greater than 1) eluted with Tris-Na2HPO4buffer at pH 8.0 reached 80.3%. A simple and convenient method using anion exchange chromatography was successfully established, which can effectively eliminate empty capsids of rAAV5 from vector capsids so that vector capsids proportion can meet the requirements of clinical application of gene therapy. Related Articles | Metrics

Select

Establishment and identification of TRPM2 gene knockout mice WANG Cunlian, LI Longfei, ZHANG Ruihua, XU Mingju, XU Tong 2023, 40 (4):  114.  doi: 10.3969/j.issn.2095-1736.2023.04.114 Abstract ( 253 )   PDF   Save The aim of this study was to obtain the transient receptor potential M2 (TRPM2) gene knockout heterozygous mouse model by using CRISPR/cas9 technology. Referring to intron 2 and intron 24 sequences of Trpm2-203 in Ensembl database, sgRNA targets were selected and synthesized in vitro. Cas9 mRNA was obtained by in vitro transcription. Cas9 mRNA and sgRNA were microinjected into the fertilized eggs of C57BL/6J mice and transplanted into the fallopian tube of pseudopregnant female mice to obtain F0mice. Positive F0generation mice were mated with wild-type C57BL/6J mice to obtain F1generation. Three positive F0generation mice were obtained by sequencing PCR products. Four strains were obtained by mating between positive F0generation mice and wild-type C57BL/6J mice with a total of 27 positive F1generation mice. Verified by real time fluorescent quantitative PCR and Western Blot, it was shown that the TRPM2 knockout heterozygous mice (TRPM2+/-) were successfully constructed in this study, which provides a good animal model for the further study of the biological function of TRPM2 gene and its expression products. Related Articles | Metrics

Select

Teaching exploration for general education on Biomedical sensors based on OBE theory GUO Jin 2023, 40 (4):  119.  doi: 10.3969/j.issn.2095-1736.2023.04.119 Abstract ( 260 )   PDF   Save Since most of the students who choose the course have different majors and have no biomedical background knowledge, the teaching reforms were explored based on OBE theory from four aspects including teaching objectives, contents, modes and evaluation methods in view of the actual teaching situations of general education course on biomedical sensors. The expected learning outcomes of this course was defined clearly in terms of knowledge and ability, and the teaching contents with the application cases of the commonly used medical equipment in daily life was created. Also, diversified teaching modes, including multimedia, class discussions, product showcase, laboratory visit, combined online and offline teaching were adopted to build a multi-level assessment system. This reform aimed to break through the discipline barriers, improve students’ interest in learning this course, stimulate the students' subjective initiative in knowledge exploration and expand their horizons and knowledge, thereby improving the teaching quality. Related Articles | Metrics

Select

Construction and practice of consolidated progressive experimental teaching of animal-cell primary culture#br# PAN Qian, LI Haoqi, LIU Jinyi, HU Boqi, YANG Huikun, CHEN Wang 2023, 40 (4):  123.  doi: 10.3969/j.issn.2095-1736.2023.04.123 Abstract ( 245 )   PDF   Save The experiment of animal-cell primary culture for undergraduates was reformed in order to solve the problems of students’ low success rate and insufficient training of abilities in traditional experimental teaching. Accordingly, a consolidated progressive experimental teaching mode was constructed and practiced. Aseptic technique and primary culture by enzymatic disaggregation, these important and widely applicated technologies, were practiced a lot. Primary culture by explantation and immunocytochemistry was phased to deepen the experiment teaching. Beyond that, students carried out the autonomous protocol designs, which were increasingly difficult. Extensibility, depth, comprehensiveness and design, therefore, were increased progressively with experiments. Students’ experiment results and survey results about the teaching effect showed that this consolidated progressive experimental teaching mode effectively enhanced their success rate. Their abilities, including practice, cooperation, analysis/solution of problems, exploration and innovation, were strengthened. It effectively solved the above issues in traditional experimental teaching and correspondingly improved the quality of experimental teaching. Related Articles | Metrics

Select

Teaching design of the experiment “screening for anti-inflammatory agents by detection of pyroptosis based on a high content screening model” #br# RUAN Banfeng, CHEN Liuzeng, WU Ke, ZHANG Min 2023, 40 (4):  127.  doi: 10.3969/j.issn.2095-1736.2023.04.127 Abstract ( 261 )   PDF   Save The teaching design of the experiment “screening for anti-inflammatory agents by detection of pyroptosis based on a high content screening model” was mainly introduced for the national college student innovation and entrepreneurship training program. Based on the theory of NLRP3 inflammasome activation leading to cell pyroptosis, the high content screening model was used to count the pyroptosis cells to determine the anti-inflammatory activity of the tested compound. The experimental results indicated that when NLRP3 inflammasomes were activated, cells underwent pyroptosis. PI could stain red fluorescence on pyroptosis cells, while the Hoechst 33342 could stain blue fluorescence on all cells. High content screening was used to count the cells with different fluorescence, the ratio was then used to determine the anti-inflammatory activity of the tested compound. The experiment combined the PI/Hoechst 33342 staining with high content screening, which could solve the problems of insufficient teaching funds, poor experimental conditions and so on. The experiment was convenient with low cost, the experimental results were intuitive and students could deeply, systematically and comprehensively understand the characteristics of the principle of pyroptosis. Thus, students who participated in the related innovation and entrepreneurship project of college students could further understand the basic process of new drug research and development while mastered the basic experimental skills of cell biology, effectively cultivated students' awareness of innovation and entrepreneurship and scientific literacy. Related Articles | Metrics