Journal of Biology ›› 2023, Vol. 40 ›› Issue (4): 83-.doi: 10.3969/j.issn.2095-1736.2023.04.083

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Comprehensive evaluation of the effect of different disinfectants on the surface disinfection of quinoa seeds and their germination

ZHU Lili1,2, ZHANG Yemeng1,2, LI Wancai5, ZHAO Yali6, CHEN Zhiguo1,3,4   

  1. 1. Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810008, China; 2. Chinese
    Academy of Sciences University, Beijing 100049, China; 3. Key Laboratory of Plateau Biological Adaptation and
    Evolution, Chinese Academy of Sciences, Xining 810008, China; 4. Key Laboratory of Crop Molecular Breeding of
    Qinghai Province, Xining 810008, China; 5. Huzhu County Seed Station, Haidong 810500, China; 6. Ledu District
    Seed Station, Haidong 810700, China
  • Online:2023-08-18 Published:2023-08-10

Abstract: To clarify the effect of seed surface disinfectants on quinoa, using Qingli No.11 and Qingli No.12 quinoa grains as experimental materials, five commonly used disinfectants (KMnO4, H2O2, NaClO , HgCl2and 75% alcohol) were selected to prepare different concentrations (0.1%, 0.5%, 1%, 1.5%, 2 %, 2.5% and 3%) and set different disinfection time (1, 5, 10, 15, 20, 25 and 30 min). L49 (41×72) orthogonal experiment was used to investigate the seed germination, seedling growth, embryo rupture rate, abnormal germination rate and moldy rate. The disinfection effect of different disinfectants treatment methods on quinoa seeds was comprehensively analyzed. The results showed that the types of disinfectants had obvious effects on the disinfection effect of quinoa seeds. Among them, KMnO4and NaClO had better disinfection effects. The quinoa seeds treated with HgCl2had the least pollution rate, but it had a greater toxic effect on seeds and seedlings, causing most seeds to break and germinate abnormally, and should not be used. H2O2treatment has less damage to the seeds, but the pollution rate is high, and the ideal disinfection effect cannot be achieved. According to the screening results of membership function method, it was concluded that 2.5% KMnO4treatment for 15 min was the best disinfection method for quinoa seeds.

Key words: quinoa seed, disinfection, germination, principal component analysis, membership function method

CLC Number: