生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (2): 17-.doi: 10.3969/j.issn.2095-1736.2026.02.017

• 研究报告 • 上一篇    下一篇

竹节参果实八氢番茄红素合酶基因（PjPSY2）克隆与功能验证

金　睿1,2， 唐婷婷1,2， 黄系伦1,2， 梁　娥2， 张　来2   

  1. 1. 贵州师范大学 生命科学学院， 贵阳 550025；
    2. 安顺学院 贵州省山地特色高效农业创新中心， 安顺 561000
  • 出版日期:2026-04-18 发布日期:2026-04-23
  • 通讯作者: 张来，博士，教授，研究方向为竹节参次生代谢产物调控等，E-mail：975575681@qq.com
  • 作者简介:金睿，硕士研究生，研究方向为竹节参皂苷合成调控，E-mail：1657943467@qq.com
  • 基金资助:
    国家自然科学基金资助项目（31660252）； 贵州省科技厅科技资助项目\[黔科合基础-ZK（2023）重点002\]； 安顺学院研究生科研创新计划项目（asxyyjscx202410）

oning and functional analysis of the phytoene synthase （PjPSY2）gene from the fruits of Panax japonicus

JIN Rui1,2, TANG Tingting1,2, HUANG Xilun1,2, LIANG E2, ZHANG Lai2   

  1. 1. School of Life Sciences, Guizhou Normal University, Guiyang 550025, China; 2. Innovation Center for
    Efficient Agricultural of Guizhou Mountain Characteristics, Anshun University, Anshun 561000, China
  • Online:2026-04-18 Published:2026-04-23

摘要： 克隆了竹节参果实中的八氢番茄红素合酶基因（PSY，PjPSY2），对其进行生物信息学分析和功能验证。结果表明：PjPSY2基因的开放阅读框为945 bp，编码蛋白质具有典型的八氢番茄红素合酶结构特征，并与其他已知植物PSY基因具有较高的同源性；以野生型拟南芥为受体进行遗传转化，成功筛选出阳性拟南芥株系，其幼苗茎叶呈紫红色；RT-PCR分析鉴定表明，PjPSY2基因已整合到野生型拟南芥的基因组中并在转录水平上进行了表达，表达量显著高于野生型拟南芥；测定了5种类胡萝卜素组成成分及成分含量，初步证实了PjPSY2基因通过调控类胡萝卜素生物合成影响竹节参果实呈色。研究为深入了解竹节参果实中八氢番茄红素合酶基因（PjPSY2）的分子特性和功能奠定了基础，研究结果以期为竹节参遗传改良和育种提供新的科学依据。

关键词: 竹节参, 八氢番茄红素合酶, 生物信息学, 果实呈色, 类胡萝卜素合成

Abstract: In this study, the phytoene synthase gene （PSY,PjPSY2） from the fruits of thePanax japonicusand conducted bioinformatics analysis and functional validation were cloned. The results showed that the open reading frame of thePjPSY2gene was 945 bp, encoding a protein with typical phytoene synthase structural characteristics, and exhibiting high homology with other known plantPSYgenes. RT-PCR analysis showed that thePjPSY2gene had been integrated into the genome of wild-typeArabidopsis thalianaand was expressed at the transcriptional level at significantly higher levels than in wild-type plants. Analysis of five carotenoid components and their contents preliminarily confirmed that thePjPSY2gene affects fruit coloration inP. japonicusby regulating the biosynthesis of carotenoids. This study provides a foundation for a deeper understanding of the molecular characteristics and functions ofPjPSY2gene inP. japonicusfruits, and the results offer a scientific basis for the genetic improvement and breeding ofP. japonicus.

Key words: Panax japonicus, phytoene synthase, bioinformatics, fruit colour, carotenoid synthesis

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