生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (2): 10-.doi: 10.3969/j.issn.2095-1736.2026.02.010

• 研究报告 • 上一篇    下一篇

罂粟O-甲基转移酶基因的密码子偏好性分析

桑璐茜1,2， 张　薇1,2， 吴嫣然2， 李　翠3， 吴　波2， 周嘉裕1， 廖　海1   

  1. 1. 西南交通大学 生命科学与工程学院， 成都 610031；
    2. 农业农村部成都沼气科学研究所， 成都 610041；
    3. 广西药用资源保护与遗传改良重点实验室 广西壮族自治区药用植物园， 南宁 530023
  • 出版日期:2026-04-18 发布日期:2026-04-23
  • 通讯作者: 周嘉裕，博士，副教授，研究方向为药用植物学和分子生物学，E-mail：spinezhou@home.swjtu.edu.cn；吴波，博士，副研究员，研究方向为微生物合成生物学与代谢工程，E-mail：wubo@caas.cn；周嘉裕和吴波为共同通信作者
  • 作者简介:桑璐茜，硕士研究生，研究方向为生物与医药，E-mail：sangluxi8671@163.com
  • 基金资助:
    广西壮族自治区药用植物园/广西药用资源保护与遗传改良重点实验室开放课题（KL2023KF04）； 广西重点研发计划项目（桂科AB23026092）

Codon bias of O-methyltransferase genes in Papaver somniferum L.

SANG Luxi1,2, ZHANG Wei1,2, WU Yanran2, LI Cui3, WU Bo2, ZHOU Jiayu1, LIAO Hai1   

  1. 1. College of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China; 2. Biogas Institute
    of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China; 3. Guangxi Key Laboratory of Medicinal
    Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China
  • Online:2026-04-18 Published:2026-04-23

摘要： 借助Codon W与EMBOSS等工具分析68条罂粟O-甲基转移酶（O-methyltransferase，OMT）基因的相对密码子使用度（RSCU）、有效密码子数（ENc）、密码子适应指数（CAI）、碱基组成等相关参数，再结合中性绘图、ENc-plot和PR2-plot分析，确定影响密码子偏好性的因素。结果显示，罂粟OMT基因的ENc平均值为48.94，CAI平均值为0.22，表明其密码子偏好性较弱。罂粟OMT基因密码子的GC含量平均值为0.40，呈现GC1s＞GC2s＞GC3s的分布趋势，表明罂粟OMT基因整体偏好A/U碱基，尤其是第3位碱基；且GC3s与ENc值存在正相关，显示GC3s会显著影响罂粟OMT基因的密码子偏好性强弱与表达水平。中性绘图、ENc-plot和PR2-plot分析显示，自然选择是影响罂粟OMT基因密码子偏好性的主要因素。罂粟OMT基因有17个优势密码子，它们均以A/U结尾，其中AGA为最优密码子。研究结果为罂粟OMT基因的密码子优化及罂粟生物碱的基因工程合成提供理论依据。

关键词: 密码子偏好性, O-甲基转移酶, 罂粟, 最优密码子

Abstract: In this study, Codon W and EMBOSS tools were adopted to determine relevant parameters, including relative synonymous codon usage (RSCU), effective number of codons (ENc), codon adaptation index (CAI), and base composition, of 68Papaver somniferumL. O-methyltransferase (PsOMT) genes. Furthermore, neutrality plot, ENc-plot, and PR2-plot were used to identify the predominant factor shaping codon usage patterns. The results showed weak codon usage bias, and the low expression level of PsOMT genes, with the average ENc values of 48.94 and average CAI values of 0.22. The average GC content of 0.40 exhibited a descending pattern of GC1s>GC2s>GC3s with an uneven distribution, suggesting preferential usage of Adenine (A) / Uracil (U)-ending codons, especially at the terminal codon. The results of neutrality plot, ENc-plot, and PR2-plot indicated that natural selection functioned as the predominant force shaping codon usage patterns. Among PsOMT genes, there were 17 optimal codons with A/U as the terminal codon, of which AGA was the most frequent. These findings provide insights for codon optimization of PsOMT genes, thereby facilitating the enhanced synthesis ofP. somniferumL. alkaloid through genetic engineering.

Key words: codon usage bias, O-methyltransferase;Papaver somniferumL., optimal codon

中图分类号: