关键词: 莲, 脂氧合酶, 基因家族鉴定, 生物信息学, 表达分析

Abstract: In this study, theLOXgene family inNelumbo nuciferawas identified and analyzed, and its expression patterns in various tissues were quantified using RT-qPCR. The results revealed that the identified 9LOXgene family members in theN. nuciferagenome belong to the 9-LOX and 13-LOX subfamilies. Subcellular localization predicted that the 9 proteins were primarily located in the cytoplasm or chloroplast. Secondary and tertiary structure predictions of the proteins showed that LOX proteins were predominantly composed of α-helices and random coils, with most being acidic proteins. Gene structure and conserved motif analysis revealed divergence among evolutionary branches but conservation within subfamilies. The promoter regions of theNnLOXgenes were rich in stress response, light response, and hormone response elements. Expression pattern analysis revealed thatNnLOX1exhibited a higher expression level in the stamens,NnLOX2was specifically expressed in the lotus seeds,NnLOX4showed significant expression in the petals, andNnLOX8andNnLOX9were primarily expressed in the lotus rhizomes. These findings suggested that the expression of lotusLOXgenes was tissue-specific, which provided a theoretical basis for further clarifying the function of theN. nucifera LOXgene and its role in plant physiological processes.

Key words: Nelumbo nucifera, lipoxygenase, gene family identification, bioinformatics, expression analysis