生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (2): 59-.doi: 10.3969/j.issn.2095-1736.2025.02.059

• 研究报告 • 上一篇    下一篇

橡胶树HbFER基因家族成员鉴定及其响应炭疽菌胁迫的表达分析

岳柳君， 安　邦   

  1. 海南大学 热带农林学院， 海口 570228
  • 出版日期:2025-04-18 发布日期:2025-04-14
  • 通讯作者: 安邦，博士，教授，研究方向为植物与病原菌互作，E-mail:anbang@hainu.edu.cn
  • 作者简介:岳柳君，硕士研究生，研究方向为植物与病原菌互作，E-mail:patris@foxmail.com
  • 基金资助:
    国家自然科学基金项目(32160594， 32260042)

Identification of the FERONIA gene family in rubber trees and expression analysis in response to stress from Colletotrichum gloeosporioides

YUE Liujun, AN Bang   

  1. School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
  • Online:2025-04-18 Published:2025-04-14

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摘要： 为深入研究橡胶树中FERONIA(HbFER)的功能，运用生物信息学方法从橡胶树基因组中筛选鉴定出31个HbFER家族成员，并对其理化性质、基序、结构域以及染色体定位进行详细分析。结果显示，这31个HbFER不均匀地分布在橡胶树的9条染色体上，编码蛋白大小为453至900 aa。序列分析显示，所有HbFER蛋白均包含Malectin-like结构域，大多数还含有PKc-like结构域。通过种内和种间共线性分析，发现在橡胶树中的HbFER存在3组共线性基因对，并且橡胶树与水稻之间的基因共线性关系较为显著，表明FER在不同类群和物种间经历了不同的进化历程，导致基因功能出现分化。表达谱分析发现，在受到胶孢炭疽菌侵染时，橡胶树叶片中大部分HbFER的表达量有不同程度下降，而个别基因表达量上升，表明橡胶树HbFER家族不同基因在应对病原菌侵染时可能发挥着差异化作用。综上所述，结果为进一步研究HbFER在橡胶树抗病中的作用提供一定的参考，也为橡胶树抗病分子育种奠定基础。

关键词: 橡胶树, 类受体激酶, FERONIA, 表达模式, 生物信息学

Abstract: To further study the function of FERONIA (HbFER) inHevea brasiliensis, the paper used bioinformatics methods to screen and identify 31HbFERfamily members from theH. brasiliensisgenome, and conducted detailed analysis of their physicochemical properties, gene sequences, domains, and chromosome location. The results showed that these 31 HbFERs were unevenly distributed on the nine chromosomes ofH. brasiliensis, encoding proteins ranging from 453 to 900 amino acids in size. Sequence analysis revealed that all HbFER proteins contained Malectin-like domains, and most of them also contained PKc-like domains. Through intra-species and inter-species collinearity analysis, it was found that there were three pairs of collinear genes forHbFERinH. brasiliensis, and the gene collinearity relationship betweenH. brasiliensisand rice was relatively significant, indicating that FER had undergone different evolutionary processes among different groups and species, and the gene functions have differentiated. Expression profile analysis revealed that the expression levels of most HbFERs inH. brasiliensisleaves decreased to varying degrees when infected withColletotrichum gloeosporioides, while the expression levels of individual genes increased. This indicated that different genes in theHbFERfamily ofH. brasiliensismight play differentiated roles in response to pathogen infection. In summary, our results provided a certain reference for further studying the role of HbFER in disease resistance inH. brasiliensis, and laid a foundation for molecular breeding ofH. brasiliensisfor disease.

Key words: Hevea brasiliensis, receptor-like kinase, FERONIA, expression patterns, bioinformatics

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